John Garratt notes: >>Be aware that validation of IHC should be performed
if you are changing your processing protocol by adding a dye to the
reagents or to a pre-processed tissue. Be cautious!<<
I don't think safranin O would be a problem, but a good idea nonetheless!
On Mon, Jan 14, 2019 at 6:4
Be aware that validation of IHC should be performed if you are changing your
processing protocol by adding a dye to the reagents or to a pre-processed
tissue. Be cautious!
John
On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet
wrote:
> Gareth Davis asked about dyes to use
Gareth Davis asked about dyes to use to mark small GI biopsy specimens to
make sure they're recovered during embedding.
I've had good results marking small specimens with the solution of safranin
O that's used in the microbiologists' Gram stain. Go to the micro lab and
ask for a small amount of it
a Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 10 January 2019 9:15 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Opinion on dye on biopsies
So, I work in a small GI lab, and I put Eosin in my first formalin on my
processor. My biopsies are very small and
So, I work in a small GI lab, and I put Eosin in my first formalin on my
processor. My biopsies are very small and this helps, somewhat, to see the
specimens for embedding and cutting. But, unfortunately, the esophagus
tissues do not absorb the eosin much. Anyway, the hospital lab I work,
part-t
