Are you allowing them to come up to the temperature of the cryostat before
cutting?
-Original Message-
From: Gerard Spoelstra via Histonet
Sent: Tuesday, September 26, 2023 3:45 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Stored frozen fatty muscle tissue
EXTERNAL
Hi Histonetters
I’ve been trying to cut frozen fatty muscle tissue from 2018/2019 stored at
-80C. I managed to cut some sections from the same tissue on fresh frozen
tissue in 2018. I suspect that storage may contribute to the difficulty to cut
this. I have yet touse dry ice to further lower