Ion Exchange Decal containers will change your experience forever.
-Original Message-
From: Gudrun Lang via Histonet
Sent: Friday, January 26, 2024 3:32 AM
To: 'Chakib Boussahmain'
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Bone samples
Hi,
In my opinion
Hi,
In my opinion the hardness of the decalcified blocks is often rather due to the
paraffin-processing than the residual calcium. Especially when the tissue is
decalcified really long. The hardness comes from the dehydration and "cooking"
of collagen fibers. So additional decal will not help
Embed the bones diagonally in your molds if you're able (size depending) as
this will allow for the greatest amount of paraffin support. Trim them very
slowly, and if need be, place the blocks into the same "slow decal" solution
for additional amount of time sufficient to enable better
Dear Orla,
Post fixation, we have stored our bone specimens in 1x PBS while having them
sent out for MicroCT analysis. We have also stored them in 1x PBS at 4°C post
fixation when necessary until further processing without having adverse affects
on our staining. However, we chose
