I'm trying to think this through. Like Will said, if it can be shown that
you can charge immunos per block... I thought we could only charge IHC per
specimen these days. Wouldn't each stage be a different specimen? I would
think billing per block of the same stage would be over charging. Why
Hiro,
You are correct, alveolar macrophages do express less F4/80 than other tissue
macrophages. I refer you to, for example:
Zhang X, Goncalves R, Mosser DM. The isolation and characterization of murine
macrophages. Curr Protoc Immunol. 2008 Nov;Chapter 14:Unit 14.1. PubMed PMID:
19016445;
The terminology is confusing stage and per layer are not the same thing to me,
depends on how you think about it . Seems that Moh's and yes I have done this,
can be done different. You have the Dr Mohs way of getting the what we call
Donut specimen, which gets inked, nicked, embedded flat then
I am using MGMT , clone MT23.2 from Invitrogen and having problems. Does
anyone have a protocol for this antibody that is working and yielding
consistent positive results?
Thanks!
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
Good Morning all, Happy Wednesday!
I have a question and I am not finding much on my google search...First
does anyone have any papers written or studies done on the use of Eosin
to stain small biopsies or the use of eosin on the tissue processor in
the last alcohol?? I have read snippets on
We use eosin in our last 100% alcohol on the processor. It is a great help for
our person embedding to be able to see and orient the small biopsies. We have
been doing this for years and have never had any problem with the processor or
any subsequent staining that has been performed. We also
Good Morning,
I would appreciate opinions and pricing on formalin substitute from anyone who
is using it. We will be supplying our OB Dept. with this since they have
trouble handling regular formalin...
Thanks,
Gale
Gale Limron CT,HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
We also add liquid eosin (~75mL) to our dirtiest 100% to help aide the sight
of the small biopsies for embedding. You are correct though, that eosin does
fluoresce.
I understand why you would be concerned about FISH, so wanted to share this. I
have heard from fellow techs in the field that
We have had great success using Prefer from Anatech. We have used it for
outside locations with bone marrow aspirations. It is formaldelyde-free, and
comes in either pre-filled containers or by larger containers. Our pathologists
were happy with the nuclear detail.and when your docs are
We use safranin (used in Microbiology as a counterstain) on our small
biopsies. We apply a small drop during grossing. It does not affect staining of
HE, IHC or ISH. We like this because it is an intense red that doesn't leach
out in the alcohols of processor.
Cindi Robinson HT(ASCP)
Mercy
Allied Search Partners is currently looking for a qualified applicant for a
Histotechnologist or Histotechnician willing to work the third shift within
a Fort Myers, FL laboratory.
Position: Histotechnologist or Histotechnician
Schedule: Monday-Friday, Overnight hours/Third Shift hours.
Here is a paper I found:
Capillary Electrophoresis Artifact Due to Eosin
Journal of Molecular Diagnostics, Vo. 7, No. 1, February 2005
Our molecular people are studying this issue to see how big of a problem it is.
Tim Morken
Supervisor, Electron Microscopy
Department of Pathology
UC San
I have nothing more to add regarding this subject but would like to address
concerns expressed here that touch on fairness, frequency, cost and the
abilities of techs and surgeons.
This was a very rare incident involving scar tissue and tumor. Our Mohs lab
does not do immunos, our
Hello,
I am looking for a recruiter to work with me in getting back into the field of
histology. I have been out a long time but I know I can re-learn. I have an
A.A. in histotechnology and I am HT certified.
I need a recruiter that won't laugh at me (it happened in the past) and that
will
Call James Elliott at Ampian staffing. One of the best in the business. Knows
histology in and out.
(877) 229-6996
Will
Sent from my iPhone
On Jun 20, 2012, at 12:15 PM, Paula araniqks...@yahoo.com wrote:
Hello,
I am looking for a recruiter to work with me in getting back into the field
Let me get this straight. You are an HT (ASCP) living in the North
Carolina Research Triangle and you can't find a job? Hmmm, UNC Health Care
in Chapel Hill, Duke Medicine in Durham, and Rex Healthcare in Raleigh are
all looking for histotechs RIGHT NOW. And this was from a 5 second Google
We also use safranin on all our small biopsies and also our breast biopsies
with no adverse effects to any of our IHC or ISH.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cp...@x-celllab.com
-Original Message-
From:
Recruiters can be cruel. I have had them laugh at me as well, but not for the
same reasons I guess. My opinion is that the markets I have worked in, they are
glad to get someone with any background, and certainly you have shown your
willingness to do what it takes to re-enter. I think that you
Carol Freeman asks about using eosin and other dyes to mark small
specimens for better recovery during embedding.
I've found safranin O to be the best of these. Use the Gram stain
counterstain used in microbiology. You mark the specimens directly, on
those blue biopsy pads. It doesn't dissolve
Why would an elastic stain work on a positive control, but the patient did not
stain?? The patient tissue is from a temporal artery which should show some
staining due to internal control??
Thanks ahead for any input on this puzzle!
Dorothy Webb, HT (ASCP)
Hi Gale - For more than seventeen years we used a product called HistoChoice
from Amresco http://www.amresco-inc.com/ . It's worth checking out.
Dave Kemler
Histology Consultant
From: Gale Limron ga...@unionhospital.org
To: histonet@lists.utsouthwestern.edu
Carol,
Its nice to hear this isn't a regular thing. In reading your original
question, it sounded like you were excited to be charging five times for
those immunos and you were ready to argue for it. Apparently that was not
the case, you wanted more information and thoughts on the subject.
I
22 matches
Mail list logo
