Hey Tony,
We are using VMS antibodies (2 are manufactured by CellMarque) on our Ultras.
We've had to go with the Optiview detection to get satisfactory results. Let
me know if you'd like our protocols.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
600
Does anyone have a preference over Paraplast and Paraplast plus for mouse
tissues?
Thanks!!
Mike
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Hello histonetters,
Our lab is thinking about introducing plastics but we are not sure what we need
or how to set it up. Basically everything on how to set up a plastics lab. I
don't know anything about it but would love to learn. Can someone please help
us!
Dear All:
We use a Tupperware container, with a nice air/liquid-tight lid. But any
container like that will work. Our trick is to buy plastic ceiling tile
material from the hardware store. (Used in ceiling panels.) It comes in big
sheets (5 feet by 3 feet?) and is pretty cheap (~$12/sheet).
My lab is considering getting our xylene and formalin from a different company
so all of our reagents are purchase through only one company. I know we cannot
just start using these new reagents without doing some kind of validation with
our processor and stainer, but after thorough searching on
Sarah,
Wow, brings back memories of the good ol' days when we used all kinds of stuff
to make our own trays. In one lab I worked at we had a small Tupperware
container for each antibody and layed paper towels and wood rods on the bottom
to hold the slides the surface. We'd wet the paper
We are going to be running the p16 antibody from Biocare (RUO) on skin
specimens. What type of tissue should I run for a positive control? The data
sheet says normal testis..
Laurie Colbert
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Ted Pella has some options. We use the Immunostain Moisture Chambers
#21049, but there are others.
http://www.tedpella.com/glasswar_html/slidedsh.htm#21049
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We are using Biocare's ab with squamous cell ca as the control of choice. I
believe you can also use cervical dysplasia or tonsil.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org
-Original
We use bioassay dishes with 10mL pipets glued to them. The slides sit on
two pipets and you can get two rows of slides in. Cutting the pipets' ends
off is the hardest part.
I take that back, finding a glue that will hold the pipets has been a
problem lately, as rubber cement isn't made like they
We use a nice CIN3 on a cervical cone.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attachments, is for the sole use of the
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Hi...We're looking for a Histotechnologist for a 3-4 month temporary (possible
full-time) position at our very busy dermatology lab in Delray Beach, FL. THIS
IS NOT AN AGENCY. Please contact me below or send your resume if interested.
This would be a 8a-5p, full time position from
Hi
I'm looking for COX/SDH combo staining procedures for muscle enzymes we
currently do these two enzyme procedure separately and we our looking into
combining them or any idea as to what sites I may find the information.
Thanks
Helene
deg...@upstate.edu
For
Manual IHC I suggest best the Sequenza Slide Rack from Thermo Scientific.
It makes your life easier and you can save money, because you only need 100 ul
of any reactive per slide.
Here is the link to the web page.
So, I am working on some IHC stuff. I need control for mouse tissues. While I
have just about every normal tissue you could ask for, several of these
antibodies say the positive control is lung cancer, or breast cancer, or
prostate cancer. While I have all of these in human I don't have them
Hi All,
Has anyone tried to section cells cut from a trans well dish?
We have cells that are cultured and attach to a trans well membrane and our
pathologist wants to look at them on the membrane but the membrane is a thin
plastic film...see the problem.
We fixed the cells in
Jamie
We have sectioned these before and we used charged slides and did not have a
problem with section adherence. I would fix longer than 5 minutes. We would
use biopsy punches to remove the membrane from the well we would process the
disc whole between biopsy pads and bisect prior to
I was able to locate the vacancy survey that the ASCP published, but
cannot find the wage survey. Does anyone have information? It was
supposed to be published in the November 2012 issue of LabMedicine.
Thanks,
Jennifer MacDonald
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Here is our technique:
Cytochrome Oxidase - SDH
Use
1. Defects of cytochrome oxidase activity
2. Demonstration of mitochondria
Underlying Principle
Combining the COX with the SDH can demonstrate COX negative fibres. These may
be SDH positive and may be ragged red fibres. 3 of the 13
Easier way to do this stain...since you do these two stains separately incubate
the slide in a the COX solution for 1 hour rinse in distilled water and
incubate in SDH solution for 1 hr. rinse 5min in physiological saline, 10min in
10% formalin saline, rinse in 15% ethanol then mount with
I guess every other year.
This is 2010
http://labmed.ascpjournals.org/content/42/3/141.full.pdf+html
The last couple of years vacancy wage from ASCP site
http://www.ascp.org/functional-nav/career-center
Joelle Weaver MAOM, HTL (ASCP) QIHC
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