[Histonet] Nitroblue Tetrazolium Chloride (NBTC)
Laurie - Tru Sigma/Aldrich. I used to get mine from them. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Type II-A Alpha amylase derived from Bacillis species
I was wondering if anyone is using this enzyme. If so, where do you buy it? Is it extremely expensive? Could you share your procedure for preparation? Janet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alpha amylase
Sorry, I was referring to glycogen digestion and PAS staining. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Amputation transport
Does anyone have a transport/ carrier for transporting amputations.? We have specimens funneled into one site for grossing, and need to transport amputations AK and BK. Thanks for any help! This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Position opening up in Pensacola
Hi Histonetters! We have someone retiring soon and will have a position for a histotech coming up. We do about 12,000 cases a year( 50,000 or so blocks). We have a Benchmark XT IHC, Dako special stainer and we are in the middle of getting a Vantage tracking system. We are like family here, close and care about each other. We work to tend to our patients and we also laugh together ( usually at me, but that's ok, I can hold my own :-) If any of you are interested reply, call or send me a resume. Thanks. Have a great week! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 - All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Amputation transport
Try mopec -Original Message- From: Fleming, Jackie M jackie.flem...@allina.com Sent: Wednesday, October 13, 2010 7:59 AM To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Amputation transport Does anyone have a transport/ carrier for transporting amputations.? We have specimens funneled into one site for grossing, and need to transport amputations AK and BK. Thanks for any help! This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Amputation transport
Good Morning to All, We have an off site hospital and the courier has a large deep plastic storage bin, like you get at Walmart, that he brings the amputations in. It is a blue bin (of course, not clear). It works very well. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fleming, Jackie M Sent: Wednesday, October 13, 2010 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amputation transport Does anyone have a transport/ carrier for transporting amputations.? We have specimens funneled into one site for grossing, and need to transport amputations AK and BK. Thanks for any help! This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.soud...@wchsys.orgmailto:ronda.soud...@wchsys.org * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please
Re: [Histonet] Optimal processing for prostate needle biopsies
How long are you pre-processing the prostate biopsies before processing on the XPress120? We use the XPress50 we fix the biopsies in Hollandes for 2 hours, then wash for 20 minutes, pre-processing solution for 10 minutes then process. We use Harris Hematoxylin in our HE and morphology is good. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Gupta, Nilesh ngup...@hfhs.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:23 PM Subject: [Histonet] Optimal processing for prostate needle biopsies I have a few questions regrading processing of prostate needle biopsies. 1. What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3. What is the best HE stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which HE is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital == CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
Phyllis: The time required in NBF is not 8 hours only, that is exactly what the regulations are trying to avoid, i.e. incomplete fixation. René J. --- On Wed, 10/13/10, Phyllis Thaxton dch...@yahoo.com wrote: From: Phyllis Thaxton dch...@yahoo.com Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Kuhnla, Melissa melissa.kuh...@chsli.org, Mahoney,Janice A janice.maho...@alegent.org, Mike Pence mpe...@grhs.net, Joyce Cline joyce.cl...@wchsys.org, histonet@lists.utsouthwestern.edu Date: Wednesday, October 13, 2010, 10:32 AM We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.soud...@wchsys.orgmailto:ronda.soud...@wchsys.org * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] FW: Joint Commission seeks NSH Member Response
Hey Histonetters. How about all of us getting involved in providing some input to the Joint Commission on their accreditation standards with regard to Histopathology. Apparently, there has not been a great response from the histology community to this request. This is our opportunity to impact the way we are looked at by this accreditation agency. Thanks, Steve From: Carrie Diamond [mailto:car...@nsh.org] Sent: Tuesday, October 12, 2010 9:28 PM To: Feher, Stephen Subject: Joint Commission seeks NSH Member Response Dear Stephen: The Joint Commission is requesting your response to the new standards' revisions, especially in the area of histopathology. The Joint Commission Laboratory Accreditation Program recently revised the existing laboratory accreditation standards and developed new standards in several specialty areas. These changes were made in response to feedback from customer surveys conducted in the fall of 2009, as well as an expert panel review in collaboration with the ASCP. More information on these revisions and the field review response process, please visit: http://www.jointcommission.org/Standards/FieldReviews/ http://www.jointcommission.org/Standards/FieldReviews/ The changes in these standards will directly impact the day to day activities for Joint Commission accredited laboratories. Participation in this survey gives you the opportunity to have an influence on your accreditation standards and elements of performance. The survey was opened on Sept. 8, 2010 and will be available until October 20, 2010. At this time, the Joint Commission has received very few responses for the histopathology section, so we are asking for your help to spread the word and to gather responses for the survey. Thank you, Carrie Diamond, Executive Director On behalf of the Board of Directors ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
Have you validated this processing? Leaving the tissue in 70% alcohol for 48 hours is not standard, and thus requires all of the hassles associated with validation. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Wednesday, October 13, 2010 7:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.soud...@wchsys.orgmailto:ronda.soud...@wchsys.org * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for
RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% From: Phyllis Thaxton [mailto:dch...@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.soud...@wchsys.orgmailto:ronda.soud...@wchsys.org * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health
[Histonet] Formalin Fixation - HER2 testing
Hopefully, ASCO and CAP will relax their fixation guidelines for HER2 testing in the near future to reflect that prolonged fixation in formalin has little, if any, affect on HER2 protein overexpression (my personal experience). Another article (see below) just appeared in the October issue of the American Journal of Clinical Pathology that supports this observation. Please keep in mind that making sure that the tissue doesn't dry out, minimizing the cold ischemic time to under 1 hour, and submitting thin (2-3 mm) sections for histological processing are probably more important than time in formalin. Ibarra JA and Rogers LW: Fixation time does not affect expression of HER2/neu. Am J Clin Pathol 2010;134:594-596. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Nitroblue Tetrazolium Chloride (NBTC)
Just Google: nitroblue tetraqzonium chloride. Lots of sources. Cheers, Fred Monson -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, October 12, 2010 6:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nitroblue Tetrazolium Chloride (NBTC) Does anyone know where I can get this dye? It is to be used by a research doc - he plans on putting the tissue in this dye and then into 10% formalin. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than normal processing time in 70% (ie. more than 1 hr). -Original Message- From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% From: Phyllis Thaxton [mailto:dch...@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.soud...@wchsys.orgmailto:ronda.soud...@wchsys.org * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if
RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, October 13, 2010 7:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% From: Phyllis Thaxton [mailto:dch...@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect
[Histonet] relaxation of her2 standards
Don't hold your breath. I have had multiple conversations with at least one of the primary individuals who set the standards and i have not detected ANY willingness to modify the standards and that includes a hard and fast insistence on good validation for ANYTHING that differs from standard processing. If you are not in compliance you are asking for a world of hurt (which may extend to the patient). Of course, your other comments about the tissue management are right on. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Type II-A Alpha amylase derived from Bacillis species
Sigma-Aldrich has it.j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jankeep...@gmail.com Sent: Wednesday, October 13, 2010 09:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Type II-A Alpha amylase derived from Bacillis species I was wondering if anyone is using this enzyme. If so, where do you buy it? Is it extremely expensive? Could you share your procedure for preparation? Janet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bladder Diverticuli
If TUR is 88307 and resection is 88309, what CPT code do you use for bladder diverticuli? Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
Rene, 8 hours is what is on the processor. We keep a log book on all breast tissue. The OR nurse documents on the label; the time of excision (placed in 10% NBF) . The gross assistant documents the time of dissection, Then the time the tissues are placed on the processor. Our fixation times for breast average about 12 to 14 hours. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Rene J Buesa rjbu...@yahoo.com To: MelissaKuhnla melissa.kuh...@chsli.org; Janice AMahoney janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu; Phyllis Thaxton dch...@yahoo.com Sent: Wed, October 13, 2010 9:39:21 AM Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Phyllis: The time required in NBF is not 8 hours only, that is exactly what the regulations are trying to avoid, i.e. incomplete fixation. René J. --- On Wed, 10/13/10, Phyllis Thaxton dch...@yahoo.com wrote: From: Phyllis Thaxton dch...@yahoo.com Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR To: Kuhnla, Melissa melissa.kuh...@chsli.org, Mahoney,Janice A janice.maho...@alegent.org, Mike Pence mpe...@grhs.net, Joyce Cline joyce.cl...@wchsys.org, histonet@lists.utsouthwestern.edu Date: Wednesday, October 13, 2010, 10:32 AM We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.soud...@wchsys.orgmailto:ronda.soud...@wchsys.org * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of
[Histonet] Hepatitis C Immuno staining in FFPE Liver
Hi Histonet, I have been trying to work up HCV antibody without much success. Anyone out there successfully doing this that would like to share their protocol with me? I have many cases that have been diagnosed with HCV infection, so the control tissue is not an issue. We are using the Dako Flex Detection. Thank you in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC OOps wrong secondary
Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Amputation transport
If you go to a general store like Fred Meyers, Target...you can get the plastic containers that hold wrapping paper rolls that are opaque and put appropriate stickers on them for less than $20.00 I have been using 3 of them for years now. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 1-503-251-6266 ex. 10246 booze...@ah.org Liz Chlipala l...@premierlab.com 10/13/2010 7:10 AM Try mopec -Original Message- From: Fleming, Jackie M jackie.flem...@allina.com Sent: Wednesday, October 13, 2010 7:59 AM To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Amputation transport Does anyone have a transport/ carrier for transporting amputations.? We have specimens funneled into one site for grossing, and need to transport amputations AK and BK. Thanks for any help! This message contains information that is confidential and may be privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail and delete the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC OOps wrong secondary
Yes René J. --- On Wed, 10/13/10, Kimberly Tuttle ktut...@umm.edu wrote: From: Kimberly Tuttle ktut...@umm.edu Subject: [Histonet] IHC OOps wrong secondary To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, October 13, 2010, 12:07 PM Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
All, I've been watching this thread for a couple of days now and would like all to know that had they attended workshop 17, provided they were in Seattle, it would have been most insightful regarding all these guidelines. It was titled Quality Biospecimens for Personalized Molecular Healthacare It addressed fixation as well as cold ischemia time. Dr. Robb also commented that as a governor for CAP,there will be CAP guidelines for tissue banking and procurement at some point in time. I only have the handout but James Robb, the speaker, would have is e-mail listed in the program we received in Seattle (it's at home, not here at work) I can look it up or if someone has it handy, please chime in for those interested. Unofortunately, this was an underpopulated workshop, but very informative. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Wednesday, October 13, 2010 10:05 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR OK I usually do not like to chime in on this, but here I go. How can a true validation of a specific target be obtained if the wiggle room is 6 to 48 hr, or 8 to 72 hr. Where is the precision and accuracy on the results for this testing if you are going to be varying process for the weekend vs weekday? This is the flaw in the guidelines in my perspective, when this much time is allowed it is like anything else. We are going to go the path of least resistance to change, instead of what is right. I know that as techs we always want the best, but are pushed to produce next day. Techs for years have been saying more fixation is needed on tissue. Well enough of that. What we do is we hold at 12 hours of fixation for all specimens no matter what? We document ischemic cold time through our LIS, to include time placed in formalin, and time of first cut. We feel that all specimens need the same fixation times. This is imperative to standardize the process, but once again we also have our processors set up in such a way that they come off at different times and our production of H an E is in sync with this. It might sound like a lot, but we get most of our work done around 96 to 97 % of cases within 24 hours or less using conventional processing techniques. With the future relying more and more on, patient centered care, through personalized medicine, we need to really look on how we can do the optimal requirements, not do the minimal requirements to reach our goals. Jesus Ellin -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, October 13, 2010 7:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% From: Phyllis Thaxton [mailto:dch...@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE:
[Histonet] pathology reports
When you have results from an outside lab, i.e. flow results, bone marrows, ect. How do you integrate this into your path report? It is a verbatim copy inside the report in the same format as the outside lab? Or can it be a copy with the results in a different format? A discussion has occurred within in or transcription department over this matter. We do attach the outside report to the hard copy report in our paper file. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking Grossing/Histo Tech
We are seeking a qualified candidate for the position of Grossing/Lead Histo Tech in our Chattanooga location. We are a state of art laboratory using the Ventana Vantage system to maintain specimen integrity; we also have Ventana special stainers and the Benchmark Ultra and XTs for our Immunohistochemistry stains. We offer a friendly working environment along with an excellent benefit package. You may obtain more information about this position at CareerBuilders.com. We also have an opening for a cytotechnologist at our Pathology office on the Erlanger Health System campus in Chattanooga, TN. Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlas...@pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Urinary bladder diverticula
Joyce Weems in Atlanta asks: If TUR is 88307 and resection is 88309, what CPT code do you use for bladder diverticuli? A transurethral resection of the prostate (TURP) is coded 88305 no matter how many blocks. A TUR of the bladder (presumably a TURBT, that is, a TUR of a bladder tumor) is 88307. A cystectomy specimen for cancer (resection) is 88309. There are no specific instructions for a diverticulum of the urinary bladder. Diverticula of the GI tract are however coded 88305, and I would code a urinary tract diverticulum (bladder or urethra) as 88305 also. The singular is diverticulum, the plural is diverticula. There is no such word as *diverticuli. If you don't have a copy of the anatomic pathology CPT codes, 2010 edition, I can send you a PDF of a scan of those pages. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 83, Issue 19
We are not open on weekends and worked out a deal with our core l= ab that is open 24/7. When we have a breast on the processer we willrun a Sat. program that ends at 21:00. They added a task on their dai= ly checklist to come up and take the tissues off at that time. They j= ust sit them on the counter and they cool - just like they were being embed= ded. When we come in on Monday we just sit them in the embedding cent= er for 15 minutes and they warm up enough to start embedding. No harm= to tissues and no one has to come in. Cathy= Crumpton HT(ASCP), Histology Lead Tuality Community Hospital Hillsbo= ro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
Fixation prior to the processor will not exce4ed 12 hrs. That is why we program the processor for 36...not to exceed 48. From: Mike Pence [mailto:mpe...@grhs.net] Sent: Wednesday, October 13, 2010 11:04 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than normal processing time in 70% (ie. more than 1 hr). -Original Message- From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% From: Phyllis Thaxton [mailto:dch...@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941
RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR
If our cases are needle cores they are almost immediately put into formalin from the patient. That time is recorded by the nurse or the clinician that took the specimen. The larger breast samples are received fresh from the OR and are immediately grossed either by a PA or resident. Those samples are examined, sliced (to expose the surface area of the specimen) and placed into formalin. That is when the fixation time is recorded for the larger specimens. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bill B. [bill...@mindspring.com] Sent: Wednesday, October 13, 2010 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately. Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: Fixation prior to the processor will not exce4ed 12 hrs. That is why we program the processor for 36...not to exceed 48. From: Mike Pence [mailto:mpe...@grhs.net] Sent: Wednesday, October 13, 2010 11:04 AM To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR How much time is the tissue in formalin prior to going in the processor? Your total time in formalin can not exceed 48 hr. And you will still need to validate your process if you hold your tissue longer than normal processing time in 70% (ie. more than 1 hr). -Original Message- From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org] Sent: Wednesday, October 13, 2010 9:45 AM To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR For the weekend, we have our processor set for 36 hours in formalin and then a hold in 70%. This allows for complete fixation and cuts down on prolonged time in 70% From: Phyllis Thaxton [mailto:dch...@yahoo.com] Sent: Wednesday, October 13, 2010 10:32 AM To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We run a weekend (Friday til Monday AM) breast run where the tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in order to complete processing on Monday morning. So far no problems. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: Kuhnla, Melissa melissa.kuh...@chsli.org To: Mahoney,Janice A janice.maho...@alegent.org; Mike Pence mpe...@grhs.net; Joyce Cline joyce.cl...@wchsys.org; histonet@lists.utsouthwestern.edu Sent: Tue, October 12, 2010 12:02:32 PM Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I disagree. Prolonged formalin fixation (over 48 hrs), diminishes signals -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 12:05 PM To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -Original Message-
[Histonet] FW: How to remove Hematoxilin
Hi histoworld, I would like to repeat my staining on the slides already coverslipped but need to remove hematoxilin first. How to remove hematoxilin? Is it need to repeat Antigen retrieval? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] I agree with Jose, etal.
Thanks for your response, Jose, I agree with your points. I see these guidelines as the first attempt to standardize something that has numerous variables. How can we expect to have the manufacturers of these markers offer (or be required by the FDA) consistent results when our end of this process is all over the board? An ER, PR Her2 that we both buy from the same manufacturer should work the exact same way in my lab as it does in yours, IF we both process in the exact same way. I doubt very much if histology and IHC testing will ever be as exact as a clinical chemistry assay for example, but it's a start. I forsee the time gap in formalin shortening as labs get used to this first step. This would mean more validation, but since we should be doing validation each year, then this may not be such a giant task. Becky Orr CLA,HT(ASCP)QIHC Technical Specialist Anatomic Pathology NorthShore University HealthSystem 847-570-2771 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Training labs in the San Francisco Bay Area?
To anyone in the San Francisco Bay area, generally northern half: Is anyone interested in training a neophyte for histotechnology? A person in San Francisco contacted me about learning histology. He came and observed in our lab and is very interested but needs a lab to train in. He is looking at online histology programs in the meantime. He does not have a degree in biology/chemistry (it is in literature) but he did work summers in a lab during high school and college and actually did limited histology at that time. If you can help him out contact me directly. No need to post back to Histonet. Thanks! Tim Morken Supervisor, Histology, IPOX UC San Francisco Medical Center Box 1656 1600 Divisidero St, B217 San Francisco, CA 94115 USA 415.514.6042 (office) 415.885.7409 Fax tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC OOps wrong secondary
Thanks to everyone who responded. I removed the coverslip, and ran it back to water and re applied the secondary, dab and counterstain. It worked like a charm. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. Kimberly Tuttle ktut...@umm.edu 10/13/2010 12:07 pm Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2010 Focus on IHC one day event comes to NJ
The date is set, the topics are in place, and the money is just right, all we needis you to attend. When: Friday, November 5, 2010 Where: Somerset Medical Center 110 Rehill Ave Somerville, NJ 08876 *2010 Focus on IHC* presented by NJSH and sponsored by BioCare Medical, LLC $10.00(members) $40.00 (Non-members) gets you: - 6 CEU's - Breakfast, Lunch and Snacks - Great learning experience Hope to see you there, Pedro Louro President (New Jersey Society for Histotechnology) Co-chair Membership Committee ___ *Meeting Schedule * 7:30-8:30AM Registration and Continental Breakfast * 8:30-12:00 *AM Session with Coffee Break * Seminars * Mouse Models and IHC; Linda Dean Antibodies 2010; Fatima Natar * OR ** Wet Workshop * Multiplex Staining in the Anatomic Laboratory; Tara Kennedy * 12:00-1:00 *Lunch (wraps, salad, soup, dessert) * 1:00-4:30 *PM Session with Coffee Break * Seminars * CAP Regulations; Terry Murphy Validation in the IHC Laboratory; George Hoernig * OR ** Wet Workshop * Rapid In Situ Hybridization; Will Chappell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
