[Histonet] autofluorescence
Any information on current methods to counter autoflourescence in formalin fixed paraffin processed tissues would be much appreciated. Many thanks Richard Edwards Leicester University U.K ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
I would just like to thank everyone for their response back on IHC certification. What a great resource for our field (Histology). Again thank you everyone. Debora Probst Columbus Regional, Columbus Ga. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eosin
Once upon a time I heard that eosin fluoresces is this true? = ; What color does it show up? Could this be used as a sort of back gr= ound stain so you can tell exactly how many cells are in the field? Sarah Goebel, B.A., HT (ASCP) Histotechnician = = XBiotech USA Inc. 8201 East Riversid= e Dr. Bldg 4 Suite 100 Austin, Texas= 78744 (512)386-2907 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Block release
Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block release
Hello, Our institution does release blocks, the requirement is that a patient consent is signed and we have an MTA (Material Transfer Agreement) For the study and appropriate IRB's in place for the study. It is a lot to set up, but after this is done for one study any following studies are easier to handle. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block release
Good Morning to All, We, as often as possible, try not to release blocks for studies or legal cases. Most studies will let you submit 10-20 unstained recut slides on Plus slides. On legal cases, we always give recuts if there is enough tissue. When slides will not suffice, we will send the block and keep a record of the block being sent. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin
Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if you observe a solution with transmitted light. René J. --- On Mon, 10/18/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote: From: sgoe...@xbiotech.com sgoe...@xbiotech.com Subject: [Histonet] Eosin To: histonet@lists.utsouthwestern.edu Date: Monday, October 18, 2010, 10:53 AM Once upon a time I heard that eosin fluoresces is this true? = ; What color does it show up? Could this be used as a sort of back gr= ound stain so you can tell exactly how many cells are in the field? Sarah Goebel, B.A., HT (ASCP) Histotechnician = = XBiotech USA Inc. 8201 East Riversid= e Dr. Bldg 4 Suite 100 Austin, Texas= 78744 (512)386-2907 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] clinical trial blocks
The fundamental philosophy that we have is that we do not want to stand in the way of the patient receiving any kind of treatment. That being said, we also have the policy that whether or not we own the material (patients are actually suppose to sign an admission consent that says they give up any ownership rights), we are clearly the legal custodians of the material, and as such are responsible for insuring that no irreparable distruction occurs. So, we prefer to not send out blocks unless the clilnical trial says there are no alternatives. We contact them and ask if we can send recuts from which they can obtain the information they desire. We also review what we have. If we have only one section, we cut a recut for the file before sending out any blocks. If we have only one block, with not much in it, we again discuss the issue with the trial coordinator. We do insist on return of the block, and assuming that there is an outside review of the material, we insist on a copy of the report. We also have the patient sign a release informing them that they need to understand that this process may exhaust any tissue that would be useful for some other new treatment which may come up down the line. Since we have to pull this material for review and usually make recuts, we bill the clinical trial. Years back, they never had any budget allowances for this, and it was always a hassle. More recently, we get a lot less BS. They know they have to pay, and they do. Unfortunately, nothing is free any more. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Autofluorescence
Richard, we had trouble with autofluorescence in brain tissue of the elderly. We have found that 1% Sudan Black B in 70% EtOH for 2 minutes before the secondary antibody works very well. Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 jabiederm...@uams.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, October 18, 2010 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 83, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Re: Histonet Digest, Vol 83, Issue 20 (Weems, Joyce) 2. RE: AFB (Weems, Joyce) 3. RE: Re: Histonet Digest, Vol 83, Issue 20 (histot...@imagesbyhopper.com) 4. Re: AFB (Lee Peggy Wenk) 5. autofluorescence (Edwards, Richard E.) 6. RE: IHC FILD (Stephanie Rivera) 7. (no subject) (Debora Probst) 8. Eosin (sgoe...@xbiotech.com) 9. Block release (Bernice Frederick) 10. RE: Block release (Helen Fedor) 11. RE: Block release (wanda.sm...@hcahealthcare.com) 12. Re: Eosin (Rene J Buesa) 13. clinical trial blocks (Tench, Bill) -- Message: 1 Date: Sun, 17 Oct 2010 18:46:54 -0400 From: Weems, Joyce jwe...@sjha.org Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 To: histot...@imagesbyhopper.com histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 92ad9b20a6c38c4587a9febe3a30e16403a6810...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=us-ascii I posted it when I found out about it last October. Sorry to everyone that didn't see it! j -Original Message- From: histot...@imagesbyhopper.com [mailto:histot...@imagesbyhopper.com] Sent: Saturday, October 16, 2010 08:17 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Joyce, THANK YOU for the link! :o) Now I wonder, if this is over a year old, is the the first time it's been seen on Histonet, or did I just miss this important update? This is really going to help the widget count in my lab! My paths frequently want to test more than one block on their tumor cases and now I can charge for them! :o) Michelle -Original Message- From: Weems, Joyce [mailto:jwe...@sjha.org] Sent: Friday, October 15, 2010 10:45 AM To: histot...@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 http://www.flpath.org/rli2.asp Here is a link that will help.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, October 15, 2010 09:59 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Histonet Digest, Vol 83, Issue 20 Can you please provide the specific CMS update number? The website doesn't seem to be too user friendly ... thanks! On Oct 14, 2010, at 12:22 PM, Weems, Joyce jwe...@sjha.org wrote: CMS/NCCI Update Dated October 1, 2009 8. The unit of service for special stains (CPT codes 88312-88313) and immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If it is medically reasonable and necessary to perform the same stain on more than one specimen or more than one block of tissue from the same specimen, additional units of service may be reported for the additional specimen(s) or block(s). Physicians should not report more than one unit of service for a stain performed on a single tissue block. For example it is common practice to cut multiple levels from a tissue block and stain each level with the same stain. The multiple levels from the same block of tissue stained with the same stain should not be reported as additional units of service. Only one unit of service should be reported for the stain on multiple levels from the single tissue block. Additionally, controls performed with special stains should not be reported as separate units of service for the stain. -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Thursday, October 14,
[Histonet] Un related to actual histology
Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Un related to actual histology
I dressed up as Abby from CSI last year. Black wig, bright red lipstick, and a lab coat is all you need =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Un related to actual histology From: Ann Bennett ann.bennett...@yahoo.com Date: Mon, October 18, 2010 2:24 pm To: histonet@lists.utsouthwestern.edu Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Un related to actual histology
Actually it's NCIS... =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: RE: [Histonet] Un related to actual histology From: sgoe...@xbiotech.com Date: Mon, October 18, 2010 2:28 pm To: Ann Bennett ann.bennett...@yahoo.com Cc: histonet@lists.utsouthwestern.edu I dressed up as Abby from CSI last year. Black wig, bright red lipstick, and a lab coat is all you need =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Un related to actual histology From: Ann Bennett ann.bennett...@yahoo.com Date: Mon, October 18, 2010 2:24 pm To: histonet@lists.utsouthwestern.edu Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Un related to actual histology
I don't know if you're a fan of Mystery Science Theater 3000, but you could be Dr Clayton Forrester! Or you could be Dexter or House. Dexter would be fun. Better yet, be Frankenstein, the doctor. That'll confuse people who aren't aware the monster had no name. Or even, FrankenSTEEN. Stab a scapel in your thigh for full effect. Also bring a recording of horses neighing so you can say Frau Bleuker! Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Mon, Oct 18, 2010 at 5:24 PM, Ann Bennett ann.bennett...@yahoo.comwrote: Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Un related to actual histology
Ah---A woman after my own heart. I always thought of going as Bunson Honeydew or (especially) Beaker from the muppet show. I work in a clinic setting and have always thought of coming in as the nurse/ Daryl Hannah character from Kill Bill would be interesting, but I don't want to give the patients heart attacks or have all the nurses get offended and get me fired. I would also of course bring a tape recorder to play her theme as well as wearing the obligatory katana. Or even, FrankenSTEEN. Stab a scapel in your thigh for full effect. Also bring a recording of horses neighing so you can say Frau Bleuker! Emily -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Un related to actual histology
They have some really neat Dexter costumes on Showtime's website! I'm so upset that I just discovered Dexter!! I'm addicted :o) I like the Abby costume idea too. From: Emily Sours talulahg...@gmail.com To: Ann Bennett ann.bennett...@yahoo.com; histonet@lists.utsouthwestern.edu Sent: Mon, October 18, 2010 6:37:03 PM Subject: Re: [Histonet] Un related to actual histology I don't know if you're a fan of Mystery Science Theater 3000, but you could be Dr Clayton Forrester! Or you could be Dexter or House. Dexter would be fun. Better yet, be Frankenstein, the doctor. That'll confuse people who aren't aware the monster had no name. Or even, FrankenSTEEN. Stab a scapel in your thigh for full effect. Also bring a recording of horses neighing so you can say Frau Bleuker! Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Mon, Oct 18, 2010 at 5:24 PM, Ann Bennett ann.bennett...@yahoo.comwrote: Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
