[Histonet] RE: Recommendations for decal solution
5% Formic Acid Thanks Lee Ann -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Friday, October 22, 2010 4:06 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Recommendations for decal solution Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Great position for an HT in Nashville, TN
Great opportunity for a Histotechnician in a brand new laboratory! Bellmeade Dermatology in Nashville, TN is looking for a certified HT or HTL to run their newly constructed laboratory. Bellmeade Dermatology has been in the dermatology business for 18 years with 3 physicians and 2 Nurse Practitioners' . Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part time position that offers a competitive salary and the flexible hours allows you to put your own personal stamp on the laboratory . Interested applicants should contact Meredith Hale phone 214-596-2219 or through email mh...@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219 cell: 469-648-8253 fax: 972-929-9966 mh...@carisls.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Wear gloves when handling / cutting paraffin blocks.
We wear gloves we have to sterile section blocks for DNA/RNA extraction. Keeps the slides free from contamination if we are placing sections on slides, otherwise we are sticking them in a vial. All equipment associated with the sterile sectioning is cleaned between each block (block holder, blade holder, forceps etc) and gloves are changed. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahs...@brain.net.pk Sent: Saturday, October 23, 2010 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wear gloves when handling / cutting paraffin blocks. Dear All, I am looking some kind of references out there that addresses that it is not a requirement to wear gloves when handling / cutting paraffin blocks. Any help would be greatly appreciated Thank in advance, Muhammad Tahseen Senior Supervisor Histology SKMCHRC Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraffin block mailers
Hi everyone, Does anyone know of a source for packaging exclusive to mailing paraffin blocks? Vendors please feel free to reply as well. Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: paraffin block mailers
Hi Jeanine, At the NSH vendors I got a sample of a neat clear plastic holder from Source Medical Products, www.sourcemp.com, 1866-735-9965, holds 4 maybe 8 blocks for mailing, depending upon the thickness. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCZVED) Sent: Monday, October 25, 2010 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin block mailers Hi everyone, Does anyone know of a source for packaging exclusive to mailing paraffin blocks? Vendors please feel free to reply as well. Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave protocols
Hi Histonetters! I am back with some questions on protocols for microwave processing. We have the Milestone processor. For any of you out there who use the Milestone, do you find the pre-programmed processing runs reasonable for your use or have you modified them? If modified, would you be willing to share your modifications? Next thought, do you process your breast needle core biopsies/lumpectomies/mastectomies etc in the microwave? I understand that they would have to properly fix in formalin for the minimum of 6 hours, but do you then process conventionally or with the MW? Does anyone have any thoughts as to whether or not the microwave would compromise the Her2/ER/PR results? Does anyone have any other pitfalls/thoughts/concerns that I need to keep in mind when using the microwave processors? THANKS for your help! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temporary Scientific Associate, Histology, spring 2011
GNF is currently seeking a temporary Scientific Associate to join the Histology group, for the beginning of March 2011 through the end of July 2011. Perform necropsies, tissue processing, slide sectioning, routine staining, special staining, immunohistochemical staining and complex procedures necessary in preparing specimens of animal tissue in a research environment. Qualifications: Associate's or B.S. degree in a biological science or completion of a NAACLS accredited School of Histotechnology is required. American Society of Clinical Pathologist (ASCP) certification as a HT or HTL is required. Applicants must demonstrate the potential ability to perform the essential functions of the job as outlined in the position description. The employee must have 2-5 years of experience in the Histology lab performing animal techniques, a wide variety of manual histochemical and enzymatic staining, automated and manual immunohistochemistry, and automated and manual in-situ hybridization. Essential Functions: 1. Identifies significant tissue elements microscopically to determine quality of staining. 2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and frozen sections. 3. Performs Microtomy on rotary microtome, cryostat, and be able to use a sliding microtome. 4. Prepares dyes and solutions in order to perform routine, special, and complex procedures. 5. Have experience in histochemical stains and enzymatic histochemical stains. Be able to trouble shoot and correct problems with histochemical stains. Have experience in Immunohistochemical staining and other advanced histological procedures. 6. Maintains lab work area by performing preventative maintenance on instruments and equipment and keeping the work area clean and orderly. 7. Operate Slide scanning Instrumentation. Interested candidates please send a copy of your CV with a summary of research experience, and three references. Job Code: JW10-013 Contact:E-mail: j...@gnf.orgmailto:j...@gnf.org James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel858-332-4647 Fax 858-812-1915mailto:858-812-1915jwat...@gnf.org jwat...@gnf.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Saponin Technique
From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histo...@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Saponin Technique
It's been a while but I think that we used to add a little diluted glacial acetic acid to our cytology specimens to lyse the red cells. Not sure on the dilution, but maybe someone else knows. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [valerie.han...@parrishmed.com] Sent: Monday, October 25, 2010 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Saponin Technique From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histo...@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Saponin Technique
I don't know about the procedure you have but ours only uses a 1% solution of saponin, a 3% solution of calcium gluconate, and a balanced salt solution. We get the balanced salt solution from our pharmacy. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, October 25, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Saponin Technique From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histo...@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Saponin
Lots of blood can be a problem in cyto specimens, especially smears. If you are making smears from fresh specimens in particular you may elute the obscuring hemoglobin by dipping the smear directly in acid alcohol (i think 5% hcl-95% etoh). We do this frequently for CT guided FNA's (particularly of the liver and thyroid, which tend to be bloody). We also do this post facto on those smears even when they have already been stained with H and E. The red cell stroma disappears into the background). Just lift the coverslip and back up to 95%, use acid alcohol ( i think it is sold on the commercial market as differentiating agent), and then start staining process all over. With fresh specimens you can see the hemoglobin elute from the surface while you dip the slide. We typically go back into regular 95% Etoh just to get rid of the acid background (effects the blueing down the line). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A Good Human Brain Control
Hi Histonetters, I was wondering if any one could tell me where can I find a human brain amyloid control. I just need some suggestions on where to order them ( control slides) from. Please feel free to list any suggestions. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fwd: [Histonet] FW: Saponin Technique
-- Forwarded message -- From: Brandi Higgins brandihigg...@gmail.com Date: Mon, Oct 25, 2010 at 12:08 PM Subject: Re: [Histonet] FW: Saponin Technique To: Hannen, Valerie valerie.han...@parrishmed.com We use Carnoy's to lyse the red blood cells. We get thyroid and lymph node FNA's regularly and we always process the slides through the Carnoy's. The solution we use is 120 ml 95% alcohol, 60 ml chloroform, and 20 ml glacial acetic acid. This 200 ml solution is good for the regular sized container for a full rack of slides, but if you only have a few slides and not a whole rack you can use 12 ml, 6 ml, 2ml (or any ratio you want obviously) so as not to waste solution. We place slides in Carnoy's for 5 minutes. The literature says 3-5 minutes, and not to exceed 15 minutes and you may require more time in the hematoxlin following this procedure, although we have not found that to be necessary. Also please note that the solution must be prepared fresh before use. We already had glacal acetic acid on hand when we started using Carnoy's so we just had to order chloroform. Brandi Higgins, BS, H(ASCP) On Mon, Oct 25, 2010 at 11:39 AM, Hannen, Valerie valerie.han...@parrishmed.com wrote: From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histo...@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Saponin
Why use Saponin when you can get a commercially prepared solution such as Lysing 1000, which was specifically designed to remove rbc's from Cytology specimens. Lysing 1000 not only lyses the rbc's but gives you exceptionally preserved cellular material which is amiable with IHC staining. Lysing 1000 is available from Obiter Research, LLC (www.obires.com) and only costs $25 per gallon. We have used it on all of our non-Gyn and FNA specimens for over 12 years now. We use it instead of 95% alcohol for our FNA smears. We prepare a smear, air dry one for a diff quik stain and immediately immerse the other on in a Coplin jar of Lysing 1000. It removes the rbc's while immediately fixing the cells present on the slide. I recommend using plus slides so the cells don't fall off. We then rinse the needle in a container of Lysing 1000 which lyses the rbc's and leaves you with a very cellular specimen for a cell block. We have valildated it's use with IHC staining using our Ventana Benchmarks and seem to get consistently excellent staining results on our cell blocks. I highly recommend it over Carnoys and Saponin. Let me know if you have any questions. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Monday, October 25, 2010 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Saponin Lots of blood can be a problem in cyto specimens, especially smears. If you are making smears from fresh specimens in particular you may elute the obscuring hemoglobin by dipping the smear directly in acid alcohol (i think 5% hcl-95% etoh). We do this frequently for CT guided FNA's (particularly of the liver and thyroid, which tend to be bloody). We also do this post facto on those smears even when they have already been stained with H and E. The red cell stroma disappears into the background). Just lift the coverslip and back up to 95%, use acid alcohol ( i think it is sold on the commercial market as differentiating agent), and then start staining process all over. With fresh specimens you can see the hemoglobin elute from the surface while you dip the slide. We typically go back into regular 95% Etoh just to get rid of the acid background (effects the blueing down the line). Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] A Good Human Brain Control
American Matertech Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] A Good Human Brain Control From: Candice Smoots candice_cami...@yahoo.com Date: Mon, October 25, 2010 9:08 am To: histonet@lists.utsouthwestern.edu Hi Histonetters, I was wondering if any one could tell me where can I find a human brain amyloid control. I just need some suggestions on where to order them ( control slides) from. Please feel free to list any suggestions. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks everyone!
Thank you to everyone who provided input on preferences for HE stainers last week. You were all very helpful! Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Director of Clinical Lab needed can you help?
Hi Histonetters! I hope everyone is having a great day. I just got a new position that I want to tell you about . The position is Clinical Lab Director. The position is with a hospital system and you would have the lab managers from the 5 affiliated labs reporting to you. The position is in AR. Please let me know if you would be interested or know of someone who might be interested. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pouring Chemicals in Stainer
I was curious about any tricks out there for avoiding spills when setting up the stainer. We will be receiving a new stainer soon which will be taller and larger than our current one. I'm just worried I will spill chemical into the surrounding dishes. Would using wash bottles work or are there any other ideas out there? V.Avalos ADS, INC Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene Substitute
Hi all! I'm looking for recommendations for a xylene substitute for our processing and staining. We have two VIP's and a Leica stainer. We use Permount for coverslipping (manually with glass). Does anyone use a xylene substitute that you would recommend for this combination? Thank you! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene Substitute
Sheila, You might want to talk to the Leica rep. My guy was here a couple of weeks ago and they have a xylene sub and a mouting media that plays nicely with it. I don't recall the name of the product, but it might be worth talking to him about it. Good Luck! Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, October 25, 2010 3:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute Hi all! I'm looking for recommendations for a xylene substitute for our processing and staining. We have two VIP's and a Leica stainer. We use Permount for coverslipping (manually with glass). Does anyone use a xylene substitute that you would recommend for this combination? Thank you! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3214 - Release Date: 10/24/10 18:34:00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rubber cutting mats with adhesive backs
Why did they quit making them and what are others using as a replacement? Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene Substitute
We use Clear-Rite 3 (Richard Allan Scientific 6901) from Fisher Scientific in our VIP processor and for staining. I am not familiar with Permount, but we use Richard-Allan Mounting Medium (4111; also available from Fisher Scientific) that is toluene based - works great in combination with Clear-Rite 3. Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Monday, October 25, 2010 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute Hi all! I'm looking for recommendations for a xylene substitute for our processing and staining. We have two VIP's and a Leica stainer. We use Permount for coverslipping (manually with glass). Does anyone use a xylene substitute that you would recommend for this combination? Thank you! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Saponin Technique
The following has been found useful in my hands: Removal of Excess Blood Haemolysing haemorrhagic specimens cause fewer problems in identifying individual cells. The removal of red blood cells can be achieved using Ficoll-Hypaque gradient separation or using a lysis solution such as isotonic ammonium chloride (Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479.): SOLUTIONS: 1. Lysis solution Ammonium Chloride4.5g Potassium carbonate 0.5g EDTA 0.0186g Distilled water 500mls 2. Hanks medium METHOD: 1. Centrifuge bloody fluid. 2. Remove supernatant and add equal volume of lysis solution. 3. Resuspend and incubate for 5 minutes at 4oC. 4. Centrifuge, if blood still remains, then repeat from step 2. 5. Resuspend pellet in Hanks. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Tuesday, 26 October 2010 2:51 AM To: Hannen, Valerie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Saponin Technique It's been a while but I think that we used to add a little diluted glacial acetic acid to our cytology specimens to lyse the red cells. Not sure on the dilution, but maybe someone else knows. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [valerie.han...@parrishmed.com] Sent: Monday, October 25, 2010 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Saponin Technique From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histo...@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
