I'm interested in learning what labs you are using for c-kit mutation for
Melanoma. I'm told this testing is different for c-kit in other organ systems
and that labs performing c-kit may not be equipped to work up the melanoma
variant
Thank you for any light you can shed on this.
Vinnie
Dear all,
I had two antibodies made (in rabbit) for the same peptide.
It was the first time I did this : the firm designed the immunization
peptide, injected and bleed the rabbits and purified the antibody.
I have tested it on paraffin sections: all background, no staining where it
should
HistonettersI am looking for the person who wrote History of
Histology. I believe the author's name was either Mitchell or Michell?
I found it on the web once, but have lost my copy? Anyone out there
remember this article? CB
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Histonet mailing
Dear Histonet Members,
I hope you are doing well. I am a Recruiter at a highly successful and well
respected Healthcare recruiting firm. I help place Lab Professionals in
permanent positions across the country and I wanted to see if you are
interested in exploring other career
The Medical University of South Carolina now offers a distance education (DE)
option for HTL training. We currently have two DE students enrolled. The format
of the program is designed for current employees in a histotechnology
laboratory. The focus of the education is on the theory of
I have done this (or tried to do this) as well and almost ended up bald
from ripping out my hair!! My first suggestion would be to try to get
down to a 1/25 or 1/50 dilution. If the background starts to get too
high then try blocking the Fc region. You can buy commercially
available Fc
Dr. Corless and staff at Oregon Health University do this testing. We send ours
there for testing. Info at this link..
http://www.ohsu.edu/pathology/TransResLabAbout.php
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 -
Greetings,
We are wanting to switch to a Masson's Trichrome method in our lab. Is
anyone aware of a good source for a commercially prepared staining kit?
William (Bill) O'Donnell, HT (ASCP) QIHC
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847
I use to use the kit from Poly Scientific #k037. Stopped when we
automated.
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
Sent: Tuesday, January 04, 2011 8:50 AM
To:
To all histotechs in the Orlando, FL area,
I have eight gallons of Surgipath I.B.F. tissue fixative that I need to get rid
off or give to a laboratory that could use it. The expiration date is 03/24/11.
If you are interested please contact me offline.
Thank you1
Damaris E.
Hi all.
For those of you using the VIP 6, how and when do you change or rotate
reagents? I'm particularly interested in those who count cassettes as a means
of
initiating reagent changes. We have mostly small biopsy specimens but do use
sponges frequently. I'm looking into reducing our reagent
Thophil Mitchell Prudden A Manuel of Normal Histology published in 1881.
Vikki
On Tue, Jan 4, 2011 at 9:41 AM, Geoff McAuliffe mcaul...@umdnj.edu wrote:
Brian Bracegirdle wrote a good book on the history of histotechnology.
Geoff
Barone, Carol wrote:
HistonettersI am looking for
Sorry typo Theophil is the correct spellingneed more tea!
On Tue, Jan 4, 2011 at 10:34 AM, Victoria Baker bakevicto...@gmail.comwrote:
Thophil Mitchell Prudden A Manuel of Normal Histology published in 1881.
Vikki
On Tue, Jan 4, 2011 at 9:41 AM, Geoff McAuliffe
Hello Histonet,
One of the pathologists here recently pointed out some strange staining
occurring on both K9 and Fel tissue using the CD3 stain. There appeared
to be very strong staining of the intestinal epithelium, all along the
villi. The staining was comparable in strength to that of the
What are the prerequisites to get in and what kind of degree if any, is
obtained upon completion?
Sent from my Verizon Wireless Phone
- Reply message -
From: Geils, Karen Brinker brink...@musc.edu
Date: Tue, Jan 4, 2011 9:36 am
Subject: [Histonet] HTL Distance Education Program
To:
Hi Histonetters!!
I want to wish you a very Promising, Prosperous and Terrific New Year in
2011.
I recognize that in this current economy every little bit helps I
really appreciate the leads that I get from you the subscribers to my
histology bulletin and would like to give back to you so I am
Hello,
I know this is probably a long shot, but I'm trying to set up a stainer and
don't have a user manual. It was bought at auction by my company, so who knows
how old it is! It is a Microm DS 50, made by Zeiss (now Thermo Richard Allen).
I've been trying to get my hands on a manual (having
Has anyone successfully lobbied their institution for an automated coverslipper
for safety reasons?
Still coverslipping manually-stained IHC, neuro autopsy and special stains,
sometimes hundreds per day. There has to be a better way. Under budget
constraints. That's why I'm wondering if
Yes we actually used the exposure to xylene to justify the purchase of a
coverslipper in our lab. Ventilation in our lab is very poor to non-existent
and we were successful in our push for the coverslipper. I also had an
employee who was having difficulties with the fumes, etc. It has made a
I should add to my earlier post this morning:
-we do use an automated coverslipper for our routine HPS staining, which is
several hundred slides daily as well
-we do have a fumehood over the main manual coverslipping area
-we have had airborne solvent levels measured, but my concern relates to
Sorry, no help here. We have lab assistants that manually coverslip and
excellent ventilation in our newer facility. It is the position of the director
that a lab assistant is cheaper than an automated instrument! Go figure!
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
If you're worried about hands being exposed to toluene, the argument would be
to use gloves. You may find your TAT to be longer, in which case you could cite
productivity as a reason for automation.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We haven't manually coverslipped here in almost 5 years. The cost of
the coverslipper as well as the consumables is still cheaper that a lab
assistant (at a full pro-rated wage including pension benefits).
We've also been able to extend it's usefulness to other departments in
our laboratory who
Hello histonetters ~
Is there any consensus regarding how often to change processor reagents? I
am using the TBS ATP1 tissue processor and the tissue size ranges from
biopsy to 1.5cm^3.
--
Katherine Leonard
Research Associate
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Barbara,
I see your antibody was purified by the manufacturer already so I don't think
it would be useful to purify it again. It sounds as though it's not a good
antibody yet. How did the company test it, by elisa? Since your western and IP
shows no specific staining, I'm not all that
Just for further clarification, the MUSC HTL program culminates in a
post-baccalaureate certificate. The prerequisites are as follows:
1. Applicants must have a bachelor of science degree in biology, chemistry,
microbiology, or related science field from an accredited college or university
Looking for IgG4 for IHC-paraffin application. We were using it from
Invitrogen, but apparently, it is no longer available. Any help is much
appreciated.
Thanks,
Kim Shields
IHC Supervisor
Royal Jubilee Hospital
Victoria, BC
___
Histonet mailing
Eric,
I agree with Liz. There are several environmental controls that could be
put in place to help with expose to xylene. Many of these controls
would cost far less than a coverslipper - so mention the health, but put
your eggs in the productivity/cost-saving basket. -
William (Bill)
Does anyone have a Histology Supervisor competency checklist they can share
with me so I do not have to re-invent the wheel! Thank you so much, ann
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Histonet@lists.utsouthwestern.edu
Our lab has had water quality issues since moving in to a new building 2 years
ago. Would like to hear from fellow Histonetters on what type of water they
are using for DI water and what type of unit are they getting it from? First
we had issues with the carbons in the water and now it seems
Can the labs out there tell me IF and how you keep inventory of your special
stains and immuno antibodies/reagents?
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hi Everyone,
Is anyone using this antibody? If so, could you please provide the source of
where you purchase it? We would be using it on paraffin sections with Dako
instrumentation.
Thank you,
Jean Taylor, HT(ASCP)QIHC
IHC Tech.
Meriter Health Services
Madison, WI
Thanks for all the great responses. I have found a manual and lots of advice!
Thanks,
Adrienne
- Forwarded Message
From: Adrienne Anderson rennie1...@yahoo.com
To: histonet@lists.utsouthwestern.edu
Sent: Tue, January 4, 2011 12:19:13 PM
Subject: [Histonet] Need help with an old
Here's a message I was asked to post. Please don't reply to me.
Thanks, Linda M (Histonet administrator)
Please post the following job announcement. Thanks you very much!
Histology Position in Milwaukee, WI
Please visit the link to learn more about the position.
Jean,
Is it anything different than just myeloperoxidase which is basically DAB,
if you do DAB without h202 blocking it will stain all the myeloid cells
including eosinophils, maybe this ab is specific just to eo's???
Cheers,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons
I think the standard rule of thumb is 700-800 blocks?
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Now that Fisher has discontinued manufacturing/distribution of the Microprobe
system, does anyone know who if anyone still offers this?
Or does anyone have one laying about that they no longer use?
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of
Karen,
Did you contact the NSH to have them include your program on their
website?
Jennifer
Geils, Karen Brinker brink...@musc.edu
Sent by: histonet-boun...@lists.utsouthwestern.edu
01/04/2011 11:08 AM
To
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
cc
Subject
Cell Marque now has an IgG4 IVD clone. It is one of the only ones I have
found.
On Tue, Jan 4, 2011 at 11:13 AM, Shields, Kim kim.shie...@viha.ca wrote:
Looking for IgG4 for IHC-paraffin application. We were using it from
Invitrogen, but apparently, it is no longer available. Any help is
CoPath users could you please email your address to me. I would like
to talk with labs that are using CoPath and chat with you about how
you process and use some of the worklogs. We have had CoPath for many
years but I have not had updates with labs to share some of how we have
Hi Histonet Members,
I am a healthcare recruiter, specifically focused on the laboratory/pathology
space. I have openings all across the country and would be interested in
speaking with anyone interested in searching for a new job. Below is a great
new opportunity that I am working on:
Hi Eric,
I used it as an additional reason in addition to cost control. The
safety
reasons are irrefutable, but you can't really put a price tag on it until you
threaten to sue for a specific monitary amount or have OSHA threaten them with
a specific fine. Business offices don't speak
Hi Histonet,
My lab needs to switch vendors on this antibody. Where is everyone buying
Pax-2? I found on their website that Invitrogen sells an IVD rabbit poly
Pax-2 antibody. Has anyone tried this or others? What about Epitomics RUO
rabbit mono? Do all the antibodies give that cytoplasmic
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