[Histonet] Service
Hello all, I am in Austin Texas and would like to know who people use to service their equipment. I have used Biomed before and wasn't really impressed. Are there any other companies? The only thing I would really ever need service on would be the microtome (brand new Thermo 325). I'm just trying to see whether a maintenance contract would be more cost effective to pay yearly than to get someone out if something goes wrong with the tome. Since it's just a plain jane rotary microtome, usually things don't really ever go wrong so I think I know the answer, but nonetheless would like repair guy/gal information. Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Service
Being from a large large with multiple instrument types and multiple units of many instruments, I find it is worth asking the manufacturer to train your on-site BioMed personnel. Repairing and maintaining Microtomes can be successfully accomplished by properly trained individuals, manufacturer, contract or on-site. You can also seek an independent contractor, but be careful when choosing. Make sure small companies do not have to large territory or are sub-contracted to the major manufacturers as this can cause friction and create turn around time issues to get your instrument fixed. Independent contractors do a great job, just make sure you are getting the service you need. When considering having your BioMed trained, a key factor in this process is to have your BioMed department receive the same training the manufacturer provides to their own and contract service personnel. Depending on your relationship with the manufacturer, BioMed training can be negotiated and there is a wide range for the cost. The key point is you develop a different relationship with the manufacturer that is more of a partnership. We have been successful with BioMed being trained for microtomes, microscopes, conventional tissues processors and HE stainer/coverslip instruments. I do not suggest this type of service for instruments that are more complex and use a specific reagent set to operate. Once you get into that complexity, there are to many variables to consider to maintain proper performance and I believe the manufacturer is best at this level. When utilizing on-site and properly trained BioMed, down time is greatly reduced, cost of parts are nominal and ordered as needed and your total service cost can be reduced. William DeSalvo, B.S., HTL(ASCP) Date: Wed, 19 Jan 2011 08:35:05 -0600 From: sgoe...@mirnarx.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Service Hello all, I am in Austin Texas and would like to know who people use to service their equipment. I have used Biomed before and wasn't really impressed. Are there any other companies? The only thing I would really ever need service on would be the microtome (brand new Thermo 325). I'm just trying to see whether a maintenance contract would be more cost effective to pay yearly than to get someone out if something goes wrong with the tome. Since it's just a plain jane rotary microtome, usually things don't really ever go wrong so I think I know the answer, but nonetheless would like repair guy/gal information. Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Background problems with frozen liver IHC
Hi All, I do a lot of fluorescence labelling of frozen mouse tissue (mostly spleens) but recently I've been trying to do IHC staining of various mouse (and human) tissues. I'm having particular trouble with liver samples with high brown background in the hepatocytes - the vascular areas are completely clean. I've been using Vector Labs avidin/biotin blocking system and their ABC detection method along with DAB (Dako) and various biotinylated secondary antibodies. There is background from the secondary antibodies and to a lesser extent from the ABC. Any tips? Thanks Sonya ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PVP method for frozen bone sectioning
Hi all, After searching the internet I found a method for preparing mouse bones for frozen sectioning. I've been trying it out but have been having problems with the PVP coming out of solution (7.5% PVP-40T, 10% EDTA Na2 in 0.1M Tris.HCl pH6.95). I heated it to ~50-60oC to get it to dissolve but when its left in the fridge a ppt develops and the mouse femur that I have decalcifying in it is covered in large white blobs (however the decalcification does seem to be working ok). Does anyone have any experience of doing frozen bone sections this way? Any advice greatly appreciated. Thanks Sonya ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Caspase
Look...I am a plethora of questions today!! Has anyone ever used the antibody Caspase? I hear lymph node is a good positive control, but where have people purchased the antibody, and at what dilution for FFPE tissue? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Caspase
Hi Sarah, I have used Cell Signaling antibody for Cleaved Caspase-3 (Asp175) Antibody #9661 is that what you are looking to do. I used it at a 1:100 dilution for 1 hr no heat with High pH. John S. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Wednesday, January 19, 2011 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Caspase Look...I am a plethora of questions today!! Has anyone ever used the antibody Caspase? I hear lymph node is a good positive control, but where have people purchased the antibody, and at what dilution for FFPE tissue? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE Stain
Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stain
More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Stain Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stain
We have been having the same problem recently. We have tried extending the washes after the hematoxylin, agitation and adding an additional wash. Nothing has helped. It is not every slide as you say, but random ones. We are using Gill 3 and eosin from Stat Lab. We have changed the stainer and processor a number of times since this began. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Mike Pence Sent: Wednesday, January 19, 2011 1:10 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stain More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Stain Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] alternative to Bouin's in Schiff-methylene blue nucleic acid
Hello, A colleague is trying to determine the nucleic acid type of a rickettsial phage. Acridine orange with RNAse/DNAse treatment was not successful. They want to try a Schiff-methylene blue technique which requires hydrolyzing in Bouin's fluid (1 h at 60 C). Could anyone suggest another protocol which does not use picric acid (or suggest an alternative to Bouin's )? Also, any advice for nucleic acid differential staining would be appreciated. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street. Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Job Opportunities NY
Dear Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on 2 open positions with a fast paced and service-oriented company in New York, NY. This company is an innovative, commercial laboratory that specializes in performing and developing testing services that serve the technically advanced medical community with a focus on neurological disorders. They are looking to hire on for the following positions: * Experienced Histotech - NYS licensed histotech, 5+ years experience, IHC experience a plus * Histology Supervisor - NYS licensed Clinical Technology Supervisor, 5+ years experience, IHC a must They are offering an exceptional compensation package, including health, dental, life, and a 401K plan. They are expanding and looking to hire as soon as possible! If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Prometheus Healthcare Lab Openings in New York
I hope you are having a great day. My name is Brian Feldman and I am with Prometheus Healthcare. We are a nationwide executive search firm that specializes in the healthcare industry. We are committed to connecting dedicated healthcare professionals with top medical organizations nationwide. I wanted to reach out to you in regards to a few new positions that I am currently working on. I have listed some of our hottest lab positions below: 1) Hospital in NYC - Histotech night shift 12-8am 10k sign on bonus Also have available an Evening Grossing Tech hours are from 4pm-12am 2) Private lab in Suffern, NY IHC Specialist (am), Lead Histotech (8-4 T-S), Histotechs for am T-S 3-11 M-f Extremely competitive pay 3) Expanding Reference lab in Westchester County Histotech II Histology 3pm - 11:30pm T-S Histotech III IHC 12am-8:30am,M-F Histotech II IHC 7am-3:30pm, T-S Histotech II IHC 8am-4:30pm, M_F Cyto Prep Tech Cytology 10pm-6:30am, M-F 4) Hospital in New Rochelle, NY Medical Technologist Supervisor for evening shift Covering areas :Hematology, chemistry, blood bank Shift is 4:30pm to 12:30am 5) Reference lab in Teterboro, NJ Cytogenetic Technologist to work second shift (4PM start time). Must have FISH experience. 6) Full time Pathologist Assistant Hospital in Bay Shore, NY Histology tech background for a Supervisory role salary range based on experience Mornings.. 7:00am start 7) Histology tech Hospital in Bay Shore, NY Day shift, 7a-3p OR 8a-4p If you might know anyone who would be interested in any of the positions listed above I would greatly appreciate it. Happy New Year! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian mailto:br...@prometheushealthcare.com @prometheushealthcare.com http://www.prometheushealthcare.com/ www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog http://twitter.com/PrometheusBlog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Autofluorescence using Mouse Tibia Tissue
Hello Histonetters, Currently, I am having trouble achieving good immunohistochemistry results using frozen mouse tibia sections. I use the protocol as follows: 4% PFA or 10% NBF 12-24 hrs cryoprotect in 20% sucrose until tissue sinks freeze tissue in block molds using OCT compound and isopentane/dry ice slice on cryostat (anywhere from 5-100um depending on microscopy work being completed) IHC: block (2-10% normal serum in TPBS) primary overnight PBS washes secondary PBS washes fluorescent mounting media I am using Cy3 labeled antibodies and not achieving that good staining I would like. Does anybody know a protocol or some special tricks I can use to avoid the autofluorescence? I can't seem to have any luck since slicing mouse bone tissue. I know many people have problems, but many publications have achieved good results. Thanks, Branden ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] alternative to Bouin's in Schiff-methylene blue nucleic acid
The hot Bouin will be working as a fixative for the bacteria in addition to causing partial hydrolysis of DNA and (probably) complete hydrolysis and dissolution of RNA. Bouin is the traditional fixative for making nuclear material (nucleoids) of bacteria visible. See Robinow C Kellenberger E 1994 The bacterial nucleoid revisited Microbiological Reviews 58(2):211-232. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = - Original Message - From: Carla M Conway cmcon...@usgs.gov Date: Wednesday, January 19, 2011 15:17 Subject: [Histonet] alternative to Bouin's in Schiff-methylene blue nucleic acid To: histonet@lists.utsouthwestern.edu Hello, A colleague is trying to determine the nucleic acid type of a rickettsial phage. Acridine orange with RNAse/DNAse treatment was not successful. They want to try a Schiff-methylene blue technique which requires hydrolyzing in Bouin's fluid (1 h at 60 C). Could anyone suggest another protocol which does not use picric acid (or suggest an alternative to Bouin's )? Also, any advice for nucleic acid differential staining would be appreciated. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street. Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stain
Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = - Original Message - From: Scott, Allison D allison_sc...@hchd.tmc.edu Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] HE Stain To: histonet@lists.utsouthwestern.edu Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
I would strongly suggest that you include cut resistant gloves as well. These are usually plastic polymer of one sort or another. At my institution we put on a pair of nitrile gloves, then the cut resistant ones, then another pair of nitrile on size larger than we usually wear. Yes, it's a lot to have on your, but yes, the extra safety is worth it. Many vendors sell these, they are not hard to find at all. Thanks, Wanda S. Gray -- Message: 11 Date: Wed, 19 Jan 2011 10:28:36 -0600 From: sgoe...@mirnarx.com Subject: [Histonet] RE: Caspase To: lset...@childrensmemorial.org Cc: histonet@lists.utsouthwestern.edu Message-ID: d957f2a7d21959488c492a2680f9920a1c5...@svrexch.asuragen.us Content-Type: text/plain; charset=us-ascii I cc'd (is that a word?) the histonet for you. If you ever want to pose a question to everyone email: histonet@lists.utsouthwestern.edu. PPE: Of course everyone has different levels of PPE to be worn, but I think the standard rule is... Gloves Lab Coat Mask (especially if cutting frozen lung tissue, it could have TB) Eye Wear You have to remember you need all of this because once the tissue hits room temperature it is basically fresh unfixed tissue again. I have cut frozen sections with no mask before and it was all ok, but I always wore one with lung frozens. Good Luck, hope this helped Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: Setlak, Lisa [mailto:lset...@childrensmemorial.org] Sent: Wednesday, January 19, 2011 10:08 AM To: Sarah Goebel Subject: RE: Caspase Hi Sarah, I don't have an answer to your question but was wondering if you could post one for me- I've tried multiple times and it doesn't seem to go through. Would you be able to tell me how to pose a question to the whole group or could you possibly post one What type of PPE is normally worn when performing frozen sections? Thanks in advance for your help. Lisa Van Valkenberg Histology Mgr. Children's Memorial Hospital Chg,. IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Consult Fees for IHC
Greetings histo land, Hope everyone is having a great New Year. I have been asked to consult on IHC to a local firm. They asked me what my fees are. I told them we can talk about that later. I have no idea what to charge these people. I want to be fair, but I don't want to give this knowledge away (an y'all thought that this was just a petty face). Any ideas for the Texas area. This would be telephone and on site consulting for a research firm. Thanks in advance. Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Consult Fees for IHC
Joe Keep in mind that that what you charge you need to take into consideration the taxes you will be responsible for paying. Most consultants are paid a simple fee and the company paying you will not take out any taxes. So you need to account for that. I have known quite a few individuals who got themselves in a bind when it came to tax time. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, January 19, 2011 3:59 PM To: Histonet Subject: [Histonet] Consult Fees for IHC Greetings histo land, Hope everyone is having a great New Year. I have been asked to consult on IHC to a local firm. They asked me what my fees are. I told them we can talk about that later. I have no idea what to charge these people. I want to be fair, but I don't want to give this knowledge away (an y'all thought that this was just a petty face). Any ideas for the Texas area. This would be telephone and on site consulting for a research firm. Thanks in advance. Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave oven
Hi, Please don't! Microwaves are horrible for HIER. For retrieval purposes, you need to get the buffer temperature up to a point and keep it there for a length of time without the solution boiling over and leaving you with unhappy dry sections. Microwaves heat unevenly really fast then boil over. The solution to this is to nuke it then cool it then nuke it again repeating until you figure it is done. Lab grade microwaves do the same thing under a bit tighter control. This results in a wave of temperature fluctuation which is anything but standardizable (if that's a word). You would be much better off getting a vegetable steamer on the cheap side, or a standard laboratory waterbath on the expensive side. These both can allow a direct monitoring of temperature throughout the retrieval process. Pressure cookers are viable options as they don't allow the buffers to boil over. Biocare Medical has a decent one as well as temperature strips that allow you to know if the temperature got to a certain point and didn't exceed another. Honestly with all CAP's nonsensical prattle about standardisation in labs I can't understand how they allow these monstrosities in modern medical care. Message: 4 Date: Tue, 18 Jan 2011 21:59:40 +0100 From: Casper Hempel casperhem...@gmail.com Subject: [Histonet] Microwave oven To: histonet@lists.utsouthwestern.edu Message-ID: AANLkTik3+ SuZ4RF7NyjQqYKCXys60CAnEwhDrfvL=j...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Dear Histonetters We are about to purchase a new microwave oven in our lab for HIER of FFPE tissue. Do you have any recommendations? I'm only aware of EMS that sells an oven with temperature control. Any suggestions are welcome Cheers Casper ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stain
Hi Toni, I would love to speak with you about the issues that you are having, could you give me a call? I am traveling tomorrow but will be back in the office on Friday. thanks Debbie Siena Technical Manager | StatLab Medical Products Direct: 972-436-1010 x229 800-442-3573 ext 229 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, January 19, 2011 12:35 PM To: Mike Pence; Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stain We have been having the same problem recently. We have tried extending the washes after the hematoxylin, agitation and adding an additional wash. Nothing has helped. It is not every slide as you say, but random ones. We are using Gill 3 and eosin from Stat Lab. We have changed the stainer and processor a number of times since this began. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Mike Pence Sent: Wednesday, January 19, 2011 1:10 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stain More often than I would like to admit when I have seen this type of problem it has been that there is a solution out of place on the processor or the stainer. I would start there. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, January 19, 2011 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Stain Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology position, Irving, TX
Hello everyone, I will have a full time histology position available within a couple of weeks and was looking for a certified technician with grossing experience. This is a day time position and may be starting at 8 am or 9am. If anyone is interested or know someone who is interested, please contact me. Thank you very much, Gloria Cole Histology Supervisor NuePath Laboratory gc...@nueterrapathology.com gloria.c...@usa.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave oven
Amos, I think you have a few misconceptions about microwave technology. Lumping laboratory grade units in with household appliances is (in my opinion) unfair. Temperature control is essential and there are microwave processors available that do it quite well. Also, how in the world can all of the buffer solution boil out of a close container? Have you looked at the modules provided by Milestone or Hacker? They all have lids! Check out the mw credentials of a company in North Carolina called CEM. Their research and manufacturing in the field of microwave technology is some of the best in the world. There are always pros cons to be considered when making equipment purchases but I believe the large net you cast damning microwave technology is short sighted. Anyone else agree? Dorothy Dorothy Traczyk MTA Histology LLC Point Pleasant, NJ 08742 -Original Message- From: Amos Brooks amosbro...@gmail.com To: casperhempel casperhem...@gmail.com; histonet histonet@lists.utsouthwestern.edu Sent: Wed, Jan 19, 2011 6:34 pm Subject: [Histonet] Microwave oven Hi, Please don't! Microwaves are horrible for HIER. For retrieval purposes, ou need to get the buffer temperature up to a point and keep it there for a ength of time without the solution boiling over and leaving you with nhappy dry sections. Microwaves heat unevenly really fast then boil over. he solution to this is to nuke it then cool it then nuke it again repeating ntil you figure it is done. Lab grade microwaves do the same thing under a it tighter control. This results in a wave of temperature fluctuation which s anything but standardizable (if that's a word). You would be much better off getting a vegetable steamer on the cheap ide, or a standard laboratory waterbath on the expensive side. These both an allow a direct monitoring of temperature throughout the retrieval rocess. Pressure cookers are viable options as they don't allow the buffers o boil over. Biocare Medical has a decent one as well as temperature strips hat allow you to know if the temperature got to a certain point and didn't xceed another. Honestly with all CAP's nonsensical prattle about tandardisation in labs I can't understand how they allow these onstrosities in modern medical care. Message: 4 ate: Tue, 18 Jan 2011 21:59:40 +0100 rom: Casper Hempel casperhem...@gmail.com ubject: [Histonet] Microwave oven o: histonet@lists.utsouthwestern.edu essage-ID: AANLkTik3+ uZ4RF7NyjQqYKCXys60CAnEwhDrfvL=j...@mail.gmail.com ontent-Type: text/plain; charset=ISO-8859-1 Dear Histonetters e are about to purchase a new microwave oven in our lab for HIER of FFPE issue. Do you have any recommendations? I'm only aware of EMS that sells an ven with temperature control. Any suggestions are welcome heers asper __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stain
I agree that the pH might be high, but I also suggest you check your water rinse on the stainer. If you are using tap water, there can be a significant fluctuation in the quality of the water and the amount of additives and impurities present at any one time can also contribute to the mucin not being rinsed away and staining. If you are using tap water, changing to distilled or dionized water might help to improve the consistency of stain results. Good luck William DeSalvo, B.S., HTL(ASCP) From: jkier...@uwo.ca To: allison_sc...@hchd.tmc.edu Date: Wed, 19 Jan 2011 16:58:57 -0500 Subject: Re: [Histonet] HE Stain CC: histonet@lists.utsouthwestern.edu Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = - Original Message - From: Scott, Allison D allison_sc...@hchd.tmc.edu Date: Wednesday, January 19, 2011 13:01 Subject: [Histonet] HE Stain To: histonet@lists.utsouthwestern.edu Hello to all in histoland and Happy New Year. We are having issues with our HE stain. The nuclei are staining very blue to purple and the mucin is staining blue to purple-blue. It is difficult to see the nuclear detail. The mucin is obscuring things. We have not changed our process for staining or processing. The funny thing is that it is only in the Biopsy cases, and it is every few slides. The surgical cases are all right. We checked the alcohol and xylene for water, and there is not any. My tech changed out the stain and we are staining a new batch of slides. If anyone has any idea what is wrong, any help would be greatly appreciated. I have gone over our processes and nothing has changed. The reagents are the same, the staining times are the same, and the processing times are the same. We are using the Shandon Gemini stainer and VIP processor. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
