[Histonet] Tape transfer
Hello, I'm trying to cut undecalcified bone (mouse knees) by using the tape transfer method. The problem is that the area that I'm interested in (the joint with patella) is not transferred from the tape on my slide. I've used slides with different coating (also those specially made for bone) and tried different thickness. None of that seems to make a difference. Another problem is that my hand roller started to stick, so I cleaned it with 100% ethanol (as recommended) and now it sticks even more... Can anybody give me some advice? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] failed thionin staining part 2
Dear friends of Histonet, I have send already a message last week considering problems with thionin staining. I got a lot of protocols, thank you for that. We used different kind of solutions, but the problem remains, independently of the solution: when our samples go through the ethanol (50%, 70%, 90%, 100%) the thionin is completely washed of. Has anyone got the same experience? What to do about it? regards, An Eerdekens ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAS with Diastase Digestion
Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both with and without slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1.Deparaffinize and hydrate to water. 2.Place the sections labeled “with” in diastase solution preheated to 37˚C for 1 hour. Hold the sections labeled “without” in distilled water. 3.Wash in running water for 5 minutes 4.Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5.Wash in 3 changes of distilled water 6.Place in Schiff reagent for 15 minutes 7.Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8.Counterstain in Mayer’s Hematoxylin for 3 minutes. 9.Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarri...@us.army.mil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI
You're not kidding Claire! Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, February 08, 2011 5:36 PM To: Therese Cook; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Sorry Therese, but - Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and have daily contact with the pathologists and many others. Contact me for details if you need to. Bob Merril (if anyone knows him) had this job, but he has retired. Claire UW Hospital and Clinics Madison WI From: histonet-boun...@lists.utsouthwestern.edu on behalf of Therese Cook Sent: Mon 2/7/2011 9:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Slone Partners=eeks a Surgical Pathology Supervisor for our hospital client, located=n Madison, Wisconsin. This key management position, will=versee the staff and the day to day operations of this busy department. =athology Assistants or HT and supervisory experience are required. A BS=s preferred. If you are energetic, have great communication=kills, and a positive attitude, this might be the position for you. Qualified candidates should send their resume to Therese=ook at [1]there...@slonepartners.com. If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward your=esume to Tara Kochis at [2]t...@slonepartners.com. All inquiries are kept confidential. References 1. 3Dmailto:there...@slonepartners.com?subject=New%20Candidate%20Request%2 0%28Surgical%20Pathology%20Supervisor%20-%201455%29 2. 3Dmailto:t...@slonepartners.com?subject=New%20Candidate%20Request%20__ _ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette labeling
A -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 4:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette labeling
B WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Tuesday, February 08, 2011 5:02 PM To: Lee Mayhew; Histonet Subject: RE: [Histonet] Cassette labeling B Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cassette labeling
B. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Lee Mayhew lmayh...@cogeco.ca Sent by: histonet-boun...@lists.utsouthwestern.edu 02/08/2011 04:57 PM To Histonet Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cassette slide/labeling
We are looking to get a bar-coded system in our laboratory. We currently hand write all cassettes and slides. What labelers do you have and do you like them? Also does anyone have the Ventana Vantage system? If so, would you recommend Vantage? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PAS with Diastase Digestion
Spit for 5 minutes old school Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both with and without slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1.Deparaffinize and hydrate to water. 2.Place the sections labeled “with” in diastase solution preheated to 37˚C for 1 hour. Hold the sections labeled “without” in distilled water. 3.Wash in running water for 5 minutes 4.Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5.Wash in 3 changes of distilled water 6.Place in Schiff reagent for 15 minutes 7.Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8.Counterstain in Mayer’s Hematoxylin for 3 minutes. 9.Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarri...@us.army.mil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mouse staining for ATP
I am looking for a reliable method for staining mouse muscle with ATP'ase pH 4.3, 4.6 and 9.4. Any help will be greatly appreciated. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, February 09, 2011 9:05 AM To: 'Erin Sarricks'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PAS with Diastase Digestion Spit for 5 minutes old school Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both with and without slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1.Deparaffinize and hydrate to water. 2.Place the sections labeled “with” in diastase solution preheated to 37˚C for 1 hour. Hold the sections labeled “without” in distilled water. 3.Wash in running water for 5 minutes 4.Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5.Wash in 3 changes of distilled water 6.Place in Schiff reagent for 15 minutes 7.Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8.Counterstain in Mayer’s Hematoxylin for 3 minutes. 9.Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarri...@us.army.mil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] High Complexity Testing
I would just like to thank everyone for all of your comments regarding the high complexity testing issue. I obviously opened a can of worms on that one! I think I understand it a little better now as I have come to the following conclusion: Although CAP requires strict guidelines and documentation regarding optimization, validation, controls, lot testing and storage (all of which are done by a tech.), the end result is the reading of the slide by a pathologist, who gets the credit for the high complexity testing. All of our hard work that provides the slide to him/her is considered a high complexity task or specimen processing. If any of you are asked this question in the future I think you will all have a better understanding of how to answer. I'm most grateful for the input. Thanks again. Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT Postion Oklahoma City
St. Anthony Hospital currently has an excellent opportunity for an experienced Histologic Technician. This position requires Certification as an HT or HLT – or – experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.comhttp://www.saintsok.com/, Ad # 10762, or contact Anna King for additional information at (405) 272-6105. Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Caspase 3
I know I have asked about this in the past, but I'm finally getting around to optimizing my antibody (Caspase3). First, tonsil will be a good control? Second, I ordered it from Abcam that is telling me the optimal dilution is between 1/10 and 1/20. This seems a little crazy? I looked at some reviews for the antibody and other people seem to have used this dilution...wow! This is going to be an expensive antibody to use!!! Anyhoo, main thing is want to make sure that tonsil will be ok for a positive control =) Stay warm my fellow Texans...it'll be 80 on Sunday!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Opening in Atlanta, GA
Hello, my name is Caryn Cole, I am a recruiter with St Joseph's Hospital in Atlanta GA and I have an opening for a Histology Technician at our Research Institute. I have listed the job description and required qualifications about the position below. If you are not interested please feel free to pass this email along to anyone who may like to apply for this position. Job Details: Saint Joseph's Hospital of Atlanta is committed to translational research through activities in the Saint Joseph's Translational Research Institute (SJTRI). Saint Joseph's Translational Research Institute is known as a leader in the treatment of cardiac and vascular diseases, orthopedics, cancer, gastroenterology, and diabetes. SJTRI has gathered scientists, industry partners, recognized physicians, and a collaborative network of national leaders in the evolving arena of translational research. Integrating top research scientists, sophisticated research facilities, education and training, SJTRI is engaged in bold and exciting programs designed to assist in the investigation of cutting edge treatments with potential benefits to patients and the community. The Histology Tech obtains, identifies and prepares tissue at necropsy for histological processing and assists in histological processing of tissue samples. Also implements SJTRI's OSHA safety program. The ideal candidate will have an Associate degree from accredited college or university in Biology or related biomedical subject or equivalent work experience. ASCP Certification within 1 year of hire. Formal safety training by OSHA certified Safety Instructor. Two years histology experience with paraffin embedding and frozen tissue immuno-histochemistry. One year experience supporting corporate safety policies. Some MS Windows experience. Full time position. Shift: 8:30a-5:00p If interested please go to www.stjosephsatlanta.orghttp://www.stjosephsatlanta.org/ and apply. Regards, Caryn Cole Employment Specialist St Joseph's Hospital Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thionin stain
An Eerdekens overseas somewhere asks about thionin staining: An, you may have a bad batch of thionin stain. Did you just open a new jar? Here is the USA we use stains certified for use in histology by the Biological Stain Commission. Each lot has been tested by them for use in histology. Maybe you could borrow some thionin from another lab and see if that works better for you? Sometimes in my career I have come across bad jars of stain that just did not work. Michael Titford Pathology USA Mobile AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] One more thing...I feel like Columbo
So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI
Hi Claire and all: I think you are confusing this position with a different position in Madison. The supervisor in this position will oversee Histology including IHC and the gross room. There are no autopsies and no renal lab. The ideal candidate will have experience with LEAN or workflow redesign. I look forward to speaking with anyone who is qualified and would be happy to give you more details. Regards, Therese Slone Partners On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote: Sorry Therese, but - Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and have daily contact with the pathologists and many others. Contact me for details if you need to. Bob Merril (if anyone knows him) had this job, but he has retired. Claire UW Hospital and Clinics Madison WI From: histonet-boun...@lists.utsouthwestern.edu on behalf of Therese Cook Sent: Mon 2/7/2011 9:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Slone Partners=eeks a Surgical Pathology Supervisor for our hospital client, located=n Madison, Wisconsin. This key management position, will=versee the staff and the day to day operations of this busy department. =athology Assistants or HT and supervisory experience are required. A BS=s preferred. If you are energetic, have great communication=kills, and a positive attitude, this might be the position for you. Qualified candidates should send their resume to Therese=ook at [1]there...@slonepartners.com. If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward your=esume to Tara Kochis at [2]t...@slonepartners.com. All inquiries are kept confidential. References 1. 3Dmailto:there...@slonepartners.com?subject=New%20Candidate%20Request%20%28Surgical%20Pathology%20Supervisor%20-%201455%29; 2. 3Dmailto:t...@slonepartners.com?subject=New%20Candidate%20Request%20___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX:330.232.9333 www.slonepartners.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Benchmark Problems
Hello, We started having a strange problem with our Ventana Benchmark XT. Out of the blue, our slides stopped staining. With most antibodies we get no reaction, some that were very strongly positive are now only very lightly staining. They do appear to counterstain OK. Following Ventana's recommendations we completely changed out all of our bulk fluids, purged the lines, changed antibodies and DAB kits. The last shot is some kind of instrument issue that's not triggering an error. Anyone ever see anything like this? Mike Dessoye _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Benchmark Problems
Hi Mike, Did you wash the bottles and rinse them and then pump the water through the instrument before adding reagents and purging all again? We had a problem like this about 2 weeks ago. I think someone loaded the wrong bulk reagent onto the instrument. Cleaning it, purging with water and adding newly made bulk reagents fixed the problem for us. Mark On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J mjdess...@wvhcs.orgwrote: Hello, We started having a strange problem with our Ventana Benchmark XT. Out of the blue, our slides stopped staining. With most antibodies we get no reaction, some that were very strongly positive are now only very lightly staining. They do appear to counterstain OK. Following Ventana's recommendations we completely changed out all of our bulk fluids, purged the lines, changed antibodies and DAB kits. The last shot is some kind of instrument issue that's not triggering an error. Anyone ever see anything like this? Mike Dessoye _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: One more thing...I feel like Columbo
Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com [sgoe...@mirnarx.com] Sent: Wednesday, February 09, 2011 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: One more thing...I feel like Columbo
https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=L10831categoryId=81937productId=12706060 http://www.medite.de/ebp50.html?L=1 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com [sgoe...@mirnarx.com] Sent: Wednesday, February 09, 2011 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette labeling
A For filing reasons. When we file in the boxes on top of microtome they are right side up and for final filing in the plastic drawers writing is right side up. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: One more thing...I feel like Columbo
I think you can get from Thermo Scientific. Two sites found on line..j http://www.medite.de/ebp50.html?L=1 https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=L10831categoryId=81937productId=12706060 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Wednesday, February 09, 2011 11:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette labeling
B Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, February 09, 2011 11:15 AM To: 'Lee Mayhew'; Histonet Subject: RE: [Histonet] Cassette labeling A For filing reasons. When we file in the boxes on top of microtome they are right side up and for final filing in the plastic drawers writing is right side up. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cassette slide/labeling
I would highly recommend the Vantage system. We have had it in place for over 2 years and our accuracy rate went from about 94-94% to over 99.9%. We have had only 2 numbering errors in Histo in over 2 years.(due to people not following procedure correctly) The system does so much more that tracking. The statistical reports are wonderful. Please contact me off line if you want to know details. Jan Mahoney 402-717-2889 Omaha, NE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Wednesday, February 09, 2011 8:45 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cassette slide/labeling We are looking to get a bar-coded system in our laboratory. We currently hand write all cassettes and slides. What labelers do you have and do you like them? Also does anyone have the Ventana Vantage system? If so, would you recommend Vantage? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette labeling
I think they must stand on their head backwardsj:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, February 09, 2011 12:27 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette labeling For those of you that answered with B, how are you filing the blocks? Just curious. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/9/2011 9:24 AM, Bernice Frederick wrote: B Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, February 09, 2011 11:15 AM To: 'Lee Mayhew'; Histonet Subject: RE: [Histonet] Cassette labeling A For filing reasons. When we file in the boxes on top of microtome they are right side up and for final filing in the plastic drawers writing is right side up. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette labeling
Using the B method ends up with the case # right side up in the block fileunless I completely misunderstood the orientations of A and B; entirely possible! Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, February 09, 2011 11:49 AM To: Victor Tobias; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette labeling I think they must stand on their head backwardsj:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, February 09, 2011 12:27 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette labeling For those of you that answered with B, how are you filing the blocks? Just curious. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/9/2011 9:24 AM, Bernice Frederick wrote: B Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, February 09, 2011 11:15 AM To: 'Lee Mayhew'; Histonet Subject: RE: [Histonet] Cassette labeling A For filing reasons. When we file in the boxes on top of microtome they are right side up and for final filing in the plastic drawers writing is right side up. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain
[Histonet] Part/Full time Histology Openings in Suffern NY
Growing private lab searching for the following openings: IHC Specialist (am) Lead Histotech (8-4 T-S) Histotechs for am T-S 3-11 M-f Excellent pay - the highest out of any company we work with in the tri-state area! Part -time openings also available. Please contact me today for immediate consideration. Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 br...@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI
Claire and I were thinking this was an advertisement for the University of Wisconsin Hospital and Clinics Surgical Pathology manager position. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Therese Cook Sent: Wednesday, February 09, 2011 10:37 AM To: Ingles Claire Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Hi Claire and all: I think you are confusing this position with a different position in Madison. The supervisor in this position will oversee Histology including IHC and the gross room. There are no autopsies and no renal lab. The ideal candidate will have experience with LEAN or workflow redesign. I look forward to speaking with anyone who is qualified and would be happy to give you more details. Regards, Therese Slone Partners On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote: Sorry Therese, but - Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and have daily contact with the pathologists and many others. Contact me for details if you need to. Bob Merril (if anyone knows him) had this job, but he has retired. Claire UW Hospital and Clinics Madison WI From: histonet-boun...@lists.utsouthwestern.edu on behalf of Therese Cook Sent: Mon 2/7/2011 9:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Slone Partners=eeks a Surgical Pathology Supervisor for our hospital client, located=n Madison, Wisconsin. This key management position, will=versee the staff and the day to day operations of this busy department. =athology Assistants or HT and supervisory experience are required. A BS=s preferred. If you are energetic, have great communication=kills, and a positive attitude, this might be the position for you. Qualified candidates should send their resume to Therese=ook at [1]there...@slonepartners.com. If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward your=esume to Tara Kochis at [2]t...@slonepartners.com. All inquiries are kept confidential. References 1. 3Dmailto:there...@slonepartners.com?subject=New%20Candidate%20Request%2 0%28Surgical%20Pathology%20Supervisor%20-%201455%29 2. 3Dmailto:t...@slonepartners.com?subject=New%20Candidate%20Request%20__ _ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX:330.232.9333 www.slonepartners.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette labeling
We've used both systems here. Both can produce right side up filing, depending on which way the wax faces (i.e. towards you, or away from you). Because we hand label the cassettes here, I prefer to use A, because the label bevel is at the top and easier to hold that way. Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 Sebree Linda A lseb...@uwhealth.org Sent by: histonet-boun...@lists.utsouthwestern.edu 02/09/2011 08:03 AM To Weems, Joyce jwe...@sjha.org, Victor Tobias vic...@pathology.washington.edu, histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Cassette labeling Using the B method ends up with the case # right side up in the block fileunless I completely misunderstood the orientations of A and B; entirely possible! Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, February 09, 2011 11:49 AM To: Victor Tobias; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cassette labeling I think they must stand on their head backwardsj:) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, February 09, 2011 12:27 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette labeling For those of you that answered with B, how are you filing the blocks? Just curious. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/9/2011 9:24 AM, Bernice Frederick wrote: B Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, February 09, 2011 11:15 AM To: 'Lee Mayhew'; Histonet Subject: RE: [Histonet] Cassette labeling A For filing reasons. When we file in the boxes on top of microtome they are right side up and for final filing in the plastic drawers writing is right side up. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication
[Histonet] CPT code 88363
Is anyone using the above CPT code for reviewing old pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] two temps needed - day shift
Hello all- We're seeking two experienced travel temps...both situations are day shift, for up to 8 weeks. Please send resume for return call- ad...@fullstaff.org fax 800.756.3309 Permanent positions also available (perm, temp or temp-to-perm depending on related experience and a few other factors) Thanks for your interest! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE:[Histonet] IHC validation
I believe the 25 cases are for ER/PR/Her-2 testing. We have done this recently and we ran about 10 for each antibody. We have over 100 ourselves and it took quite a while. There will be some antibodies that you will be unable to find 10 slides to test, so do as many (or as few) as your Medical Director is comfortable with to validate the stain. Good luck! Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 Message: 14 Date: Tue, 8 Feb 2011 18:12:42 -0500 From: Weems, Joyce jwe...@sjha.org Subject: RE: [Histonet] IHC validation To: Liz Chlipala l...@premierlab.com, Joe Nocito jnoc...@satx.rr.com, Histonet histonet@lists.utsouthwestern.edu Message-ID: 92ad9b20a6c38c4587a9febe3a30e164081e09a...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=us-ascii But that is for receptors, correct? Do you do that for everything? Thanks, j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, February 08, 2011 17:58 To: Joe Nocito; Histonet Subject: RE: [Histonet] IHC validation Joe If you are following the recommendations from the CAP paper on IHC standardization then it would be 25 tissues (10 strong positive, 10 weak to moderate positive and 5 negative). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, February 08, 2011 3:44 PM To: Histonet Subject: [Histonet] IHC validation Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI
Right. It is NOT with the University of Wisconsin. On Feb 9, 2011, at 1:11 PM, Sebree Linda A wrote: Claire and I were thinking this was an advertisement for the University of Wisconsin Hospital and Clinics Surgical Pathology manager position. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Therese Cook Sent: Wednesday, February 09, 2011 10:37 AM To: Ingles Claire Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Hi Claire and all: I think you are confusing this position with a different position in Madison. The supervisor in this position will oversee Histology including IHC and the gross room. There are no autopsies and no renal lab. The ideal candidate will have experience with LEAN or workflow redesign. I look forward to speaking with anyone who is qualified and would be happy to give you more details. Regards, Therese Slone Partners On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote: Sorry Therese, but - Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and have daily contact with the pathologists and many others. Contact me for details if you need to. Bob Merril (if anyone knows him) had this job, but he has retired. Claire UW Hospital and Clinics Madison WI From: histonet-boun...@lists.utsouthwestern.edu on behalf of Therese Cook Sent: Mon 2/7/2011 9:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Slone Partners=eeks a Surgical Pathology Supervisor for our hospital client, located=n Madison, Wisconsin. This key management position, will=versee the staff and the day to day operations of this busy department. =athology Assistants or HT and supervisory experience are required. A BS=s preferred. If you are energetic, have great communication=kills, and a positive attitude, this might be the position for you. Qualified candidates should send their resume to Therese=ook at [1]there...@slonepartners.com. If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward your=esume to Tara Kochis at [2]t...@slonepartners.com. All inquiries are kept confidential. References 1. 3Dmailto:there...@slonepartners.com?subject=New%20Candidate%20Request%2 0%28Surgical%20Pathology%20Supervisor%20-%201455%29 2. 3Dmailto:t...@slonepartners.com?subject=New%20Candidate%20Request%20__ _ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE:877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX: 330.232.9333 www.slonepartners.com SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX:330.232.9333 www.slonepartners.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CPT code 88363
Only molecular... And I read that somewhere, but don't remember where. I'll search. j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, February 09, 2011 13:12 To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing old pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CPT code 88363
This can only be used for slide review prior to ordering molecular testing. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 662-4648 If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microm parts for microtomes, only through Thermo?
I just ordered one from VWR. It's for a thermo tome which I think will fit on microms? It was $1600. I would call a tech help and see what they would suggest. Another thought would be to call IMEB (specifically Denise deVines 18005438496). I have had lots of good luck with the company and Denise is great! They do a lot of refurbishing of equipment and maybe she could help you out or at least point you in the right direction. Ok Denise...I get a cookie with my next order right =) Note: I am in no way affiliated with IMEB or getting anything for this suggestion, I just think it's a great company and wanted to pass it along to everyone!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Wednesday, February 09, 2011 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microm parts for microtomes, only through Thermo? Hi All, I have a blade assembly that is giving grief. I would like to just adjust the set screws to fix it and will try. In checking for a new assembly I got a price of 2220 for just the blade holder. Are there any companies out there that manufacture after market products like this? Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette labeling
In Tissue Tek block cabinets, front (smallest number) to back (larger numbers) with numbers facing right side up as you pull out the drawer. Does that make sense? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Wednesday, February 09, 2011 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette labeling For those of you that answered with B, how are you filing the blocks? Just curious. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 2/9/2011 9:24 AM, Bernice Frederick wrote: B Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, February 09, 2011 11:15 AM To: 'Lee Mayhew'; Histonet Subject: RE: [Histonet] Cassette labeling A For filing reasons. When we file in the boxes on top of microtome they are right side up and for final filing in the plastic drawers writing is right side up. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
[Histonet] core histolgy recharge rates
Hello all, I am trying to get a feel for how other core histology facilities are charging. My main question is if or when technician's labor time is charged. Currently we charge a fee per sample for embedding, whether it is paraffin, plastic, or oct. Then we charge a fee per slide/grid for cutting, and fee per slide/grid for staining. Any time the technician is doing the work there is also a labor/tech time fee per hour. Any information you are willing to share is very much appreciated. Thanks, Michael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cell block fixation
We recently switched vendors for our formalin and while we have not experienced any difference with our surgical specimens, our cell blocks from body fluids have been giving us a great deal of trouble. The button that we get never seems to harden, leaving it sort of gelatinous, even if left to sit in formalin for days. We are able to get sections off of these cell blocks, however, the slides are blank by the end of the staining process. This is only a recent development that seems to coincide with the time we switched formalin vendors and it only happens with body fluid specimens (FNA specimens don't seem to give us as much trouble). The composition of the formalin is almost identical between vendors. Can anyone help me explain why this might be happening? Thank you in advance, Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI
Sorry gang: the position in Madison wasn't the one I was thinking of. Disregard warning. It is the position for the surgical path manager at UW Hospital in Madison I was talking about. Claire I think you are confusing this position with a different position in Madison. The supervisor in this position will oversee Histology including IHC and the gross room. There are no autopsies and no renal lab. The ideal candidate will have experience with LEAN or workflow redesign. I look forward to speaking with anyone who is qualified and would be happy to give you more details. Regards, Therese Slone Partners On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote: Sorry Therese, but - Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology lab, and have daily contact with the pathologists and many others. Contact me for details if you need to. Bob Merril (if anyone knows him) had this job, but he has retired. Claire UW Hospital and Clinics Madison WI From: histonet-boun...@lists.utsouthwestern.edu on behalf of Therese Cook Sent: Mon 2/7/2011 9:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI Slone Partners=eeks a Surgical Pathology Supervisor for our hospital client, located=n Madison, Wisconsin. This key management position, will=versee the staff and the day to day operations of this busy department. =athology Assistants or HT and supervisory experience are required. A BS=s preferred. If you are energetic, have great communication=kills, and a positive attitude, this might be the position for you. Qualified candidates should send their resume to Therese=ook at [1]there...@slonepartners.com. If you do not meet these qualifications, but wish to be considered for other roles in the laboratory diagnostic industry, please forward your=esume to Tara Kochis at [2]t...@slonepartners.com. All inquiries are kept confidential. References 1. 3Dmailto:there...@slonepartners.com?subject=New%20Candidate%20Request%2 0%28Surgical%20Pathology%20Supervisor%20-%201455%29 2. 3Dmailto:t...@slonepartners.com?subject=New%20Candidate%20Request%20__ _ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE:877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX: 330.232.9333 www.slonepartners.com SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX:330.232.9333 www.slonepartners.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC validation
Joe: This is what I would do; 1- run 1 control slide per antibody you have in your arsenal 2- compare the result with a control slide already in your files. 3- show both slides to the chief pathologist (after all is his/her opinion the one is going to be asked by CAP) 4- those antibodies whose positive controls reacted substantially different to those in your files are the ones you have to work with with respect to concentration or detection method. 5- never overdue it, and avoid excessive costs that usually are never appreciated. Rely always in your pathologist's opinion René J. --- On Tue, 2/8/11, Joe Nocito jnoc...@satx.rr.com wrote: From: Joe Nocito jnoc...@satx.rr.com Subject: [Histonet] IHC validation To: Histonet histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 5:43 PM Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nickel-DAB Signal Fading
If when you finished the procedure the signal was strong, it is not a problem with the antibody, it would have reacted with your working concentration, or would not have worked. I am inclined to think that the nickel solution you use to add to the DAB may have deteriorated in that batch and have caused the fading. Try some of the block to cut new sections and use a fresh nickel solution to find out what happens in 2 months time. René J. --- On Tue, 2/8/11, Amanda Madden amkmad...@gmail.com wrote: From: Amanda Madden amkmad...@gmail.com Subject: [Histonet] Nickel-DAB Signal Fading To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 6:27 PM Hello Histonetters! I am once again completely baffled, and thought you might be able to help. I run immunocytochemistry on rodent brain tissue every week, always using the exact same procedures, solutions, buffers, chromagen, and mounting medium. The only variable is the primary and secondary antibodies. Our protocol has been time tested and extremely successful. I recently ran a large batch of tissue, and the staining looked great... except that now, about two months later, the signal has faded almost to the point of being gone. I am using Nickel intensified DAB, if that helps, and coverslipping (after an alcohol series finished with xylene) with Permount. Could it be a problem with the primary antibody (one which is new to us but has seemed fine in series dilutions)? Or is it a problem with the chromagen/mounting medium? I have been storing the slides in slides boxes so I don't think it is light. Going crazy, and thankful for any advice, Amanda Madden ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PAS with Diastase Digestion
You CAN NOT use water to dissolve the diastase. You have to use diastase buffer pH7 otherwise it will not work (as in your case). René J. --- On Wed, 2/9/11, Erin Sarricks esarri...@gmail.com wrote: From: Erin Sarricks esarri...@gmail.com Subject: [Histonet] PAS with Diastase Digestion To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 9, 2011, 7:38 AM Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both with and without slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1. Deparaffinize and hydrate to water. 2. Place the sections labeled “with” in diastase solution preheated to 37˚C for 1 hour. Hold the sections labeled “without” in distilled water. 3. Wash in running water for 5 minutes 4. Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5. Wash in 3 changes of distilled water 6. Place in Schiff reagent for 15 minutes 7. Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8. Counterstain in Mayer’s Hematoxylin for 3 minutes. 9. Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarri...@us.army.mil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Don't pick lemons. See all the new 2007 cars at Yahoo! Autos. http://autos.yahoo.com/new_cars.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CPT code 88363
It is my understanding that it used for examaination and selection of retrieved archival (ie,previously diagnosed) tissue for molecular analysis (eg, KRAS mutational analysis). page 440 of the 2011 AMA CPT , professional edition. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [rcar...@harthosp.org] Sent: Wednesday, February 09, 2011 1:12 PM To: Histonet Subject: [Histonet] CPT code 88363 Is anyone using the above CPT code for reviewing old pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC validation
What a sensible process! I agree with your approach. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, February 09, 2011 3:27 PM To: Histonet; Joe Nocito Subject: Re: [Histonet] IHC validation Joe: This is what I would do; 1- run 1 control slide per antibody you have in your arsenal 2- compare the result with a control slide already in your files. 3- show both slides to the chief pathologist (after all is his/her opinion the one is going to be asked by CAP) 4- those antibodies whose positive controls reacted substantially different to those in your files are the ones you have to work with with respect to concentration or detection method. 5- never overdue it, and avoid excessive costs that usually are never appreciated. Rely always in your pathologist's opinion René J. --- On Tue, 2/8/11, Joe Nocito jnoc...@satx.rr.com wrote: From: Joe Nocito jnoc...@satx.rr.com Subject: [Histonet] IHC validation To: Histonet histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 5:43 PM Greetings Histoland, I need some help. We are about to switch IHC machines from the Richard-Allen Axiom to the Ventana Benchmark Ultra. How many slides, per antibody, do you run for the validation study? We have over 100 primary antibodies. Normally, when we work up a new antibody, we start with a titer. Once that is established, we run 10 cases to check for specificity. Hopefully we can obtain cases that are really positive, some weakly positive and some flat out negative. Once that is completed, we run 10 different tissue types to check for any unexpected cross-reactivity. The ultra holds 30 slides and we are receiving two machines. If we run 10 slides/antibody, that's going to take a while, not to mention the number of detection kits that will be used. Do you think 5 slides/antibody is sufficient? I emailed CAP last week for their take and they never returned my email (I told my medical director to hold their check for the year and see how fast they respond to that). Ah oh, don't go down that road Joe, it's unhealthy. What are your thoughts? Thanks Joe (JTT) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CPT code 88363- RE: Histonet Digest, Vol 87, Issue 18, Message-ID: 4d5292a4.7400.007...@harthosp.org
As defined at the AMA site, you have a valid use of the CPT code 88363 to claim for a review of old, previously diagnosed pathology slides prior to ordering IHC testing. If reviewing for previously DX'd slides is not enough, IHC is tagging the molecules in the cells at the binding site. AMA- Examination and selection of retrieved archival (ie, previously diagnosed) tissue(s) for molecular analysis (eg, KRAS mutational analysis) Source- https://catalog.ama-assn.org/Catalog/cpt/cpt_search_result.jsp?_requestid=51 3040 5. CPT code 88363 (Richard Cartun) 10. CPT code 88363 (Lake,Debbie) Keith M. Mikoff, HTL(ASCP)(ret) -- Message: 5 Date: Wed, 09 Feb 2011 13:12:05 -0500 From: Richard Cartun rcar...@harthosp.org Subject: [Histonet] CPT code 88363 To: Histonet histonet@lists.utsouthwestern.edu Message-ID: 4d5292a4.7400.007...@harthosp.org Content-Type: text/plain; charset=US-ASCII Is anyone using the above CPT code for reviewing old pathology slides prior to ordering IHC testing or can it only be used for slide review prior to ordering molecular testing? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax -- Message: 10 Date: Wed, 9 Feb 2011 13:34:27 -0500 From: Lake,Debbie debbie.l...@mgh.net Subject: [Histonet] CPT code 88363 To: histonet@lists.utsouthwestern.edu Message-ID: 8d6edb72cecabb4a988c5bb087b61e4f01032...@mghemail1.mgh.net Content-Type: text/plain; charset=us-ascii This can only be used for slide review prior to ordering molecular testing. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 662-4648 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QIHC
Hi all I am preparing to take the QIHC exam. Does anyone have any suggestions on what I should study? What areas does the exam concentrate on and what research material I can use. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] core histolgy recharge rates
Hi- A flat fee per slide was charged which included all *technical* preparation for the final slide. Also prior to the start of a project a cost estimate was compiled and submitted for review/approval. As portions of projects were completed we submit via the grant or project number the work completed and $$'s were deducted from that cost center. We did do a number of different types of tests/assays and if we were approached for a new type of assay then we would have to put together a cost analysis based on the needs of the project. Incorporated into that cost would be the technical/labor component also including whatever overhead percentage was required by the facility. This worked out well as I had everything done 'up front' and knew what was budgeted. We did have to submit a quarterly report of all work/$$'s to the government and by doing a flat fee it made life much easier. Vikki On Wed, Feb 9, 2011 at 1:59 PM, Michael Mashore mmash...@vapop.ucsd.eduwrote: Hello all, I am trying to get a feel for how other core histology facilities are charging. My main question is if or when technician's labor time is charged. Currently we charge a fee per sample for embedding, whether it is paraffin, plastic, or oct. Then we charge a fee per slide/grid for cutting, and fee per slide/grid for staining. Any time the technician is doing the work there is also a labor/tech time fee per hour. Any information you are willing to share is very much appreciated. Thanks, Michael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tape transfer
Dear Nele, I hear of these problems very often. It would be worth your time looking for any information on fresh frozen bone sections by Dr. Dodds, there should be a number of papers he has published on good quality bone sectioning without the use of the slow, expensive and troublesome tape method. Alan Bright www.brightinstruments.com Sent from my BlackBerry® wireless device -Original Message- From: Nele Degryse nele.degr...@ugent.be Sender: histonet-boun...@lists.utsouthwestern.edu Date: Wed, 09 Feb 2011 09:30:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape transfer Hello, I'm trying to cut undecalcified bone (mouse knees) by using the tape transfer method. The problem is that the area that I'm interested in (the joint with patella) is not transferred from the tape on my slide. I've used slides with different coating (also those specially made for bone) and tried different thickness. None of that seems to make a difference. Another problem is that my hand roller started to stick, so I cleaned it with 100% ethanol (as recommended) and now it sticks even more... Can anybody give me some advice? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS -- Teach SpamSniper if this mail (ID 01E5IwhGp) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01E5IwhGpm=9503c828d7b7c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01E5IwhGpm=9503c828d7b7c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01E5IwhGpm=9503c828d7b7c=f -- END-ANTISPAM-VOTING-LINKS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Benchmark Problems
I would also see if Ventana can send you some test slides to test the heating. At my old lab, we had a problem with the heating plates. They repaired the entire platform. Just a thought. Joe - Original Message - From: Mark Tarango marktara...@gmail.com To: Dessoye, Michael J mjdess...@wvhcs.org Cc: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 09, 2011 10:58 AM Subject: Re: [Histonet] Benchmark Problems Hi Mike, Did you wash the bottles and rinse them and then pump the water through the instrument before adding reagents and purging all again? We had a problem like this about 2 weeks ago. I think someone loaded the wrong bulk reagent onto the instrument. Cleaning it, purging with water and adding newly made bulk reagents fixed the problem for us. Mark On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J mjdess...@wvhcs.orgwrote: Hello, We started having a strange problem with our Ventana Benchmark XT. Out of the blue, our slides stopped staining. With most antibodies we get no reaction, some that were very strongly positive are now only very lightly staining. They do appear to counterstain OK. Following Ventana's recommendations we completely changed out all of our bulk fluids, purged the lines, changed antibodies and DAB kits. The last shot is some kind of instrument issue that's not triggering an error. Anyone ever see anything like this? Mike Dessoye _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: One more thing...I feel like Columbo
Sakura also has the heated forceps that plug directly into the embedding center. Weems, Joyce jwe...@sjha.org Sent by: histonet-boun...@lists.utsouthwestern.edu 02/09/2011 09:23 AM To sgoe...@mirnarx.com sgoe...@mirnarx.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] RE: One more thing...I feel like Columbo I think you can get from Thermo Scientific. Two sites found on line..j http://www.medite.de/ebp50.html?L=1 https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId=L10831categoryId=81937productId=12706060 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Wednesday, February 09, 2011 11:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tape Transfer
You Wrote (two messages) Dear Nele, I hear of these problems very often. It would be worth your time looking for any information on fresh frozen bone sections by Dr. Dodds, there should be a number of papers he has published on good quality bone sectioning without the use of the slow, expensive and troublesome tape method. Alan Bright www.brightinstruments.com Sent from my BlackBerryR wireless device -Original Message- From: Nele Degryse Nele.Degryse http://lists.utsouthwestern.edu/mailman/listinfo/histonet @t UGent.be Sender: histonet-bounces http://lists.utsouthwestern.edu/mailman/listinfo/histonet @t lists.utsouthwestern.edu Date: Wed, 09 Feb 2011 09:30:44 To: histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet @t lists.utsouthwestern.edu Subject: [Histonet] Tape transfer Hello, I'm trying to cut undecalcified bone (mouse knees) by using the tape transfer method. The problem is that the area that I'm interested in (the joint with patella) is not transferred from the tape on my slide. I've used slides with different coating (also those specially made for bone) and tried different thickness. None of that seems to make a difference. Another problem is that my hand roller started to stick, so I cleaned it with 100% ethanol (as recommended) and now it sticks even more... Can anybody give me some advice? *** First, I totally disagree that using the slow, expensive and troublesome tape method is a problem. I took Dodd's workshop and then attempted cut bone frozen sections in our lab using his methods which proved to be a nightmare, taking hours to get one decent section IF we were lucky. We never got lucky in obtaining a an intact bone section that was free from folding and totally disrupted morphology. If any method is troublesome, it is the Dodd's method plus the expense of wasting time and nothing to show for it. The only thing I have used with any success was snap freezing bone the way he taught us, but that has gone by the wayside due to the toxic hexane fumes.His way was a total failure in our laboratory and we bought the Cryojane to get decent bone sections. Cryojane requires practice, a bit of patience, fine tuning the little details for successful use and it is not as slow as people think. There are many labs who use it successfully including ours. First of all, Nele did not provide any details on how the Cryojane was being used other than the different polymer coated slides. So questions follow: What was the cryostat chamber temperature? Is this the same temperature of the sample, knife and UV light source?If the cryostat is older model, have you actually checked the temperatures? Is the bone fixed in formalin before snap freezing? And if the bone was fixed, was it cryoprotected with at least 20 to 30% sucrose (this takes a long time for NBF fixed bone)? What thicknesses did you try? Thinner is often better than thicker. Is the d profile tungsten carbide knife perfectly sharp when cutting the frozen bone sections? This means a new edge for sectioning, and very frequent reconditioning of the knife (DDK or Dorn and Hart sharpening services). Hopefully you have two knives. Have you tried multiple UV flashes? Two to three flashes usually help keep bone on the slide. Do you remove the tape INSIDE the cold cryostat environment, and at an angle from one corner of slide across the section, slowly and gently. Have you tried orienting the bone differently? Sometimes this helps, particularly when removing the tape from the bone.Is your mouse leg left articulated so you section through the bent knee joint (into patella)? It sounds as though you have a temperature problem IF things are sticking. When washing the roller, are you putting a lot of alcohol on the roller, or simply wiping the roller with the alcohol. Is the roller at the exact cutting temperature of the sample before rolling the COLD tape onto the block face? More details will help since we can't see a real time session of what you are doing. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FFPE tissue - sucrose fixing necessary?
Hello helfpul Histonetters! I am curious about your formalin-fixed paraffin embedded (FFPE) tissue protocols. If the tissue has been fixed with saline and paraformaldehyde during perfusion, then further fixed in paraformaldehyde for 24 hours, do you still utilise a sucrose wash before running it through the automatic tissue processor? Or do you not need this step? I have heard mixed responses about this and wonder if any of you have had experience with this! Thanks so much, Abbey ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Tape Transfer
Dear Colleagues Please remove my husband, Charles E Brown Jr. from you listings. My husband passed away on 1/27/11. Respectfully Mrs. Charles E Brown, Jr. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Wednesday, February 09, 2011 4:42 PM To: 'Histonet' Subject: [Histonet] RE: Tape Transfer You Wrote (two messages) Dear Nele, I hear of these problems very often. It would be worth your time looking for any information on fresh frozen bone sections by Dr. Dodds, there should be a number of papers he has published on good quality bone sectioning without the use of the slow, expensive and troublesome tape method. Alan Bright www.brightinstruments.com Sent from my BlackBerryR wireless device -Original Message- From: Nele Degryse Nele.Degryse http://lists.utsouthwestern.edu/mailman/listinfo/histonet @t UGent.be Sender: histonet-bounces http://lists.utsouthwestern.edu/mailman/listinfo/histonet @t lists.utsouthwestern.edu Date: Wed, 09 Feb 2011 09:30:44 To: histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet @t lists.utsouthwestern.edu Subject: [Histonet] Tape transfer Hello, I'm trying to cut undecalcified bone (mouse knees) by using the tape transfer method. The problem is that the area that I'm interested in (the joint with patella) is not transferred from the tape on my slide. I've used slides with different coating (also those specially made for bone) and tried different thickness. None of that seems to make a difference. Another problem is that my hand roller started to stick, so I cleaned it with 100% ethanol (as recommended) and now it sticks even more... Can anybody give me some advice? *** First, I totally disagree that using the slow, expensive and troublesome tape method is a problem. I took Dodd's workshop and then attempted cut bone frozen sections in our lab using his methods which proved to be a nightmare, taking hours to get one decent section IF we were lucky. We never got lucky in obtaining a an intact bone section that was free from folding and totally disrupted morphology. If any method is troublesome, it is the Dodd's method plus the expense of wasting time and nothing to show for it. The only thing I have used with any success was snap freezing bone the way he taught us, but that has gone by the wayside due to the toxic hexane fumes.His way was a total failure in our laboratory and we bought the Cryojane to get decent bone sections. Cryojane requires practice, a bit of patience, fine tuning the little details for successful use and it is not as slow as people think. There are many labs who use it successfully including ours. First of all, Nele did not provide any details on how the Cryojane was being used other than the different polymer coated slides. So questions follow: What was the cryostat chamber temperature? Is this the same temperature of the sample, knife and UV light source?If the cryostat is older model, have you actually checked the temperatures? Is the bone fixed in formalin before snap freezing? And if the bone was fixed, was it cryoprotected with at least 20 to 30% sucrose (this takes a long time for NBF fixed bone)? What thicknesses did you try? Thinner is often better than thicker. Is the d profile tungsten carbide knife perfectly sharp when cutting the frozen bone sections? This means a new edge for sectioning, and very frequent reconditioning of the knife (DDK or Dorn and Hart sharpening services). Hopefully you have two knives. Have you tried multiple UV flashes? Two to three flashes usually help keep bone on the slide. Do you remove the tape INSIDE the cold cryostat environment, and at an angle from one corner of slide across the section, slowly and gently. Have you tried orienting the bone differently? Sometimes this helps, particularly when removing the tape from the bone.Is your mouse leg left articulated so you section through the bent knee joint (into patella)? It sounds as though you have a temperature problem IF things are sticking. When washing the roller, are you putting a lot of alcohol on the roller, or simply wiping the roller with the alcohol. Is the roller at the exact cutting temperature of the sample before rolling the COLD tape onto the block face? More details will help since we can't see a real time session of what you are doing. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
RE: [Histonet] RE: One more thing...I feel like Columbo
Dear Histonet Colleagues My husband, Charles E Brown Jr, passed away 1/27/11 Respectfully, Mrs. Charles E Brown Jr. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Wednesday, February 09, 2011 2:47 PM To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: One more thing...I feel like Columbo Sakura also has the heated forceps that plug directly into the embedding center. Weems, Joyce jwe...@sjha.org Sent by: histonet-boun...@lists.utsouthwestern.edu 02/09/2011 09:23 AM To sgoe...@mirnarx.com sgoe...@mirnarx.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] RE: One more thing...I feel like Columbo I think you can get from Thermo Scientific. Two sites found on line..j http://www.medite.de/ebp50.html?L=1 https://www.thermoscientific.com/wps/portal/ts/products/detail?navigationId= L10831categoryId=81937productId=12706060 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Wednesday, February 09, 2011 11:38 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] One more thing...I feel like Columbo So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
