[Histonet] Contract rate
Hola peeps!! So does anyone know what the going rate for after hours contact HT work is. We are an urban research lab and it would be after hours work. We are in Austin Texas if that helps any =) Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Equipment service and repair
I am wondering if there are any service company's that will repair and service Leica equipment in northern New York (Saranac Lake)? I have a Leica VT 1000s that needs some attention and would need some occasional PM visits. Thanks for any suggestions! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Symphony Issues
This inquiry is from the lead histotech inour lab: Is anyone having problems with moisture on the slide, bubbles and misaligned coverslips or multiple coverslips. If so, what are they being told from Ventana? What have they done to resolve issues? Can you respond to her directly @ pwebs...@swmail.sw.org Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:254-724-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:254-724-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:nsea...@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech/IHC/Grossing Jobs In NY/NJ/CT areas (Permanent)
Allied Search Partners has been retained for the following searches. We have openings in Immunohistochemistry, Grossing, and Histology. Please forward this along to anyone who you know that would be interested in any of the following positions. We do offer generous referral bonuses. 1. Please email a copy of updated resume to me or fax to 888-388-7572 for a full job description. Please note: No other information about the location or organization will be given prior to receiving an updated copy of your resume. Thank you! We have the following positions available: Immunohistochemistry (IHC) Histotech LOCATION: East White Plains/Near Port Chester, NY area DEPARTMENT SCHEDULE: Tuesday-Saturday 2PM-10:20PM OR Monday-Friday 9:30pm-5pm Immunohistochemistry (IHC) Histotech LOCATION: Clifton, NJ area (Northern NJ) DEPARTMENT SCHEDULE: Monday-Friday, Day Shift Histotech LOCATION: East White Plains/Near Port Chester, NY area DEPARTMENT SCHEDULE: Tuesday-Saturday 7am-3:30PM Histotech LOCATION: Binghamton, NY (East of Ithaca) DEPARTMENT SCHEDULE: Monday-Friday Day Shift 8AM-4:30PM Grossing Histotech LOCATION: Port Chester, NY area/East White Plains DEPARTMENT SCHEDULE: Monday-Friday 7pm-12am To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com http://www.alliedsearchpartners.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Symphony
We had a Symphony instrument for 6 months and had that problem - variable staining due to the humidity, bubbles, etc. Ventana engineers came to our lab and worked on the issue. Then Ventana brought a new instrument in but was never able to resolve the problem for us. We do not have a Symphony in our lab currently. It is really too bad because we really wanted it to work. The concept is the most patient safe and lean of any of the dip and dunk stain instruments. Thanks, Charlotte Kopczynski, HTL (ASCP) Pathology Manager Morton Plant Mease Health System BayCare Health System Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Equipment service and repair
Speaking of Leica VT1000S Vibrating microtome - I am looking for one, for a research facility. Let me know if you have a used one available. Thank you, Gwen Vaughn GMI, Inc. ...Instrumental to Science Buyer http://www.gmi-inc.com/ T: 1-800-745-2710 | F: 763-712-8724 | 1-800-745-2710 Sign up to GMI for deals on New Recertified Laboratory Instrumentation - Download Product GMI Catalogs -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, July 31, 2012 10:49 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Equipment service and repair I am wondering if there are any service company's that will repair and service Leica equipment in northern New York (Saranac Lake)? I have a Leica VT 1000s that needs some attention and would need some occasional PM visits. Thanks for any suggestions! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Contract rate
Is that full contact, or just sparring? Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Greetings, does anyone know of a less expensive substitute for Kim-wipes? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Old telephone book pages! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, July 31, 2012 2:23 PM To: Histonet Subject: [Histonet] (no subject) Greetings, does anyone know of a less expensive substitute for Kim-wipes? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Second that - learned that trick for cleaning the water bath surface from Pete Emanuele at AFIP a looong time ago. Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, July 31, 2012 3:05 PM To: 'Paula Pierce'; Histonet Subject: RE: [Histonet] (no subject) Old telephone book pages! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, July 31, 2012 2:23 PM To: Histonet Subject: [Histonet] (no subject) Greetings, does anyone know of a less expensive substitute for Kim-wipes? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Expert Level Cutting and Embedding
Hi, We all know there are many labs in a transitional stage or are having to deal with employee turnover at any given point in time. It takes time to find the right candidate who is the best fit for your lab and its goals. HistoCare helps to alleviate workload stress while providing embedding and microtomy support for your lab with excellent and detailed results. Our interest is in short term contract partnerships (1-90 days) and are available on short notice in most states. We provide punctual and reliable assistance to your lab. Embedding experience is top notch and we haven't met a fatty or hard bony block we couldn't cut yet. We can definitely back up these claims in person! Visit www.HistoCare.com to see photos of our work. Videos demonstrating our skills are available upon request. Please pass this info on to anyone who's having a hard time finding great team members but still need their laboratory to run efficiently and continue to meet turn around time. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Back in the Olde Days, when we gathered wax for embedding from the beehives in the back of the hospital and used the eggs from the hospital's chicken coops to make albumin as a slide adhesive (I'm being facetious here, I hope you know...), we used ScotTowels, torn in half, for cleaning water bath surfaces while cutting. I've sent this idea to Paula Pierce but it bears repeating here as I'm sure one could find very inexpensive paper towels in the pick-a-size package. I've always thought Kimwipes were too wimpy for cleaning the water surface but I apparently was spoiled by ScotTowels! And, yes, I am enjoying retirement but I rather miss the routine. I'll get over it! - Original Message - From: Linda Blazek lbla...@digestivespecialists.com To: Paula Pierce cont...@excaliburpathology.com, Histonet histonet@lists.utsouthwestern.edu Sent: Tuesday, July 31, 2012 1:05:23 PM Subject: RE: [Histonet] (no subject) Old telephone book pages! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, July 31, 2012 2:23 PM To: Histonet Subject: [Histonet] (no subject) Greetings, does anyone know of a less expensive substitute for Kim-wipes? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Done that!!! And old shiny magazines. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, July 31, 2012 3:05 PM To: 'Paula Pierce'; Histonet Subject: RE: [Histonet] (no subject) Old telephone book pages! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, July 31, 2012 2:23 PM To: Histonet Subject: [Histonet] (no subject) Greetings, does anyone know of a less expensive substitute for Kim-wipes? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Symphony Issues
Hi Nita, We had a similar problem and our Technical Service rep narrowed it down to where the coverslippers were actually manufactured. After changing out the coverslipper 3 times, he ordered a new one directly from Tuscon and we have not had the problem again. So far as humidity goes, there could be a number of issues. The first thing you need to check is with your engineers to make sure they are not pumping moisture into the air. Many hospitals do this so that is where I would start. Also, keep that filter changed regularly. I wish you luck and hope you can make it work for you. Christie Date: Tue, 31 Jul 2012 11:05:41 -0500 From: nsea...@swmail.sw.org To: histonet@lists.utsouthwestern.edu; pwebs...@swmail.sw.org CC: Subject: [Histonet] Symphony Issues This inquiry is from the lead histotech inour lab: Is anyone having problems with moisture on the slide, bubbles and misaligned coverslips or multiple coverslips. If so, what are they being told from Ventana? What have they done to resolve issues? Can you respond to her directly @ pwebs...@swmail.sw.org Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Symphony Issues
Report this to the tech support. We had to have an adjustment and also got rid of a bad lot of coverglass. they replaced it at no cost to us. --- On Tue, 7/31/12, Nita Searcy nsea...@swmail.sw.org wrote: From: Nita Searcy nsea...@swmail.sw.org Subject: [Histonet] Symphony Issues To: histonet@lists.utsouthwestern.edu, Patricia Webster pwebs...@swmail.sw.org Date: Tuesday, July 31, 2012, 12:05 PM This inquiry is from the lead histotech inour lab: Is anyone having problems with moisture on the slide, bubbles and misaligned coverslips or multiple coverslips. If so, what are they being told from Ventana? What have they done to resolve issues? Can you respond to her directly @ pwebs...@swmail.sw.org Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Can't beat phone books. Cheap, torn in half they are wider than a kimwipe, you're recycling, and providing therapy in the form of stress relief obtained tearing up phone books. Rena On Tue, Jul 31, 2012 at 2:23 PM, Paula Pierce cont...@excaliburpathology.com wrote: Greetings, does anyone know of a less expensive substitute for Kim-wipes? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] our first histology conference
Dear all we are now arrage for the first conference of our department at Faculty of veterinary medicine- Cairo University- Egypt . Within the range of 6 monthes.the details well be specified and tell you aboutit well be an amazing opportunity for you to take a trip and visit Egypt and enjoy the sunny weathercruize the River Nilevisit Khan al- Khalili market the largiest market in the world for torrists to buy exotic perfume bottels, arab clothes and amazing sauvenirs...Also visiting the Pyramids the only Ancient Wonder of the world that haven't been destroid by time and many many other interesting places So we want to enrich our new conference by 2-3 speakers who are going to visit Egypt and participate in our conference as speaker - lecturer for any topic related to our amazing feild..and realy a good opportunity to made a channel of connection between us in Egypt and you..Please don't hisitat to replay to me whatever your position and the favorite topic you would like to share us with it...and don't miss the opprtunity to introduce yourself to us and enjoing the trip in Egypt..Also for companies which would like to share us email me for arrangment all advice from you well be appreciated thanks Dr. Mohamed abd El Razik Ass. Lec. of Vet. Histology Faculty Of Veterinary Medicine Cairo University - Egypt http://www.facebook.com/mohamed.abdelrazik.9 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Proteolytic enzyme pretreatment before immunostaining
Dear Histonetters, Could anyone explain about the difference between proteolytic enzymes for immuostaining? We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did manual staining. At the moment I am using Leica's BondMax autostainer and their enzyme pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the pretreatment condition would be affected by the concentration of enzymes, pH, temperature, incubation time etc. My question is that do they have different mode of action on tissues? I am helping a research project and our antibody includes various clones of Integrin 6 subunit and uPAR. I have tried enzyme pretreatment with autostainer and manual staining with Proteinase K (Dako). It seems that some antibodies work better with certain enzymes. I mean that some antibodies work well after BondMax enzyme treatment, but some antibody works better with proteinase K pretreatment manually. I am using the same polymer detection system (Leica Microsystem) for both methods. I would like to find an enzyme which works for both of our antibodies at the same time. Thank you. Young Kwun Senior Hospital Scientist Immunohistochemistry Dept. of Anatomical Pathology Concord Repatriation General Hospital Concord NSW 2139 Australia 02-9767-6075 (Tel) 02-9767-8427 (Fax) young.k...@sswahs.nsw.gov.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Proteolytic enzyme pretreatment before immunostaining
Enzyme pretreatment, and all steps in epitope retrieval, should follow the same quality control steps as antibody incubation and antibody concentration. Enzyme pretreatment is not magic, however the kinetics involved are very difficult to predict. Epitopes are typically chemical shapes within tertiary protein structure, however they can involve secondary structure and quartinary structure. The purpose of enzyme pretreatment is to get the right epitope in the right configuration so it can be recognized by the antibody. Heat retrieval is meant to break formalin cross linkages, but enzyme pretreatment actually eats away at part of the protein (depending on the protease). Too little protease treatment and it does nothing, too much and the epitope is destroyed. The bottom line, novel antibodies need to be validated with numerous retrieval methods. If it is deemed that a protease is better, numerous times and numerous concentrations should be tried -- even at different temperatures. Finally, there is no reason to believe that different novel antibodies will require the same pretreatment, however, a common pretreatment may be sufficient (though possibly not optimal) for each antibody. One last thing, if you know more about the nature of the antigen used to create the antibody, you may be able to predict the required pretreatment -- talk to the primary investigator. Will Chappell, HTL(ASCP)QIHC Anatomic Pathology Supervisor Children's Hospital of Orange County On Jul 31, 2012, at 8:41 PM, Young Kwun wrote: Dear Histonetters, Could anyone explain about the difference between proteolytic enzymes for immuostaining? We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did manual staining. At the moment I am using Leica's BondMax autostainer and their enzyme pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the pretreatment condition would be affected by the concentration of enzymes, pH, temperature, incubation time etc. My question is that do they have different mode of action on tissues? I am helping a research project and our antibody includes various clones of Integrin 6 subunit and uPAR. I have tried enzyme pretreatment with autostainer and manual staining with Proteinase K (Dako). It seems that some antibodies work better with certain enzymes. I mean that some antibodies work well after BondMax enzyme treatment, but some antibody works better with proteinase K pretreatment manually. I am using the same polymer detection system (Leica Microsystem) for both methods. I would like to find an enzyme which works for both of our antibodies at the same time. Thank you. Young Kwun Senior Hospital Scientist Immunohistochemistry Dept. of Anatomical Pathology Concord Repatriation General Hospital Concord NSW 2139 Australia 02-9767-6075 (Tel) 02-9767-8427 (Fax) young.k...@sswahs.nsw.gov.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
