RE: [Histonet] Levels for Gastic and Esophogeal Bx's
And I should have added that we do two unstained at level 2 - if needed for alcian blue/PAS and H. pylori on all gastrics. We only do them when ordered. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, January 09, 2015 11:07 AM To: 'Eileen Akemi Allison'; Histonet Subject: RE: [Histonet] Levels for Gastic and Esophogeal Bx's Akemi, it is really up to the pathologists to decide what they want. In some cases there is literature promoting certain standards (e.g, Hirschprung's) but in most cases it is just the comfort level of the person reviewing the case. We have a large variety of levels and unstained protocols but for standard biopsies it is two levels on one slide, no unstained. We only cut unstained if they are ordered as part of a protocol or ordered specifically. We do not cut extra unstained just in case. We had tens of thousands of unstained slides sitting in files just in case so I convinced them to stop that practice. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Friday, January 09, 2015 6:07 AM To: Histonet Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx’s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Levels for Gastic and Esophogeal Bx's
Akemi, it is really up to the pathologists to decide what they want. In some cases there is literature promoting certain standards (e.g, Hirschprung's) but in most cases it is just the comfort level of the person reviewing the case. We have a large variety of levels and unstained protocols but for standard biopsies it is two levels on one slide, no unstained. We only cut unstained if they are ordered as part of a protocol or ordered specifically. We do not cut extra unstained just in case. We had tens of thousands of unstained slides sitting in files just in case so I convinced them to stop that practice. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Friday, January 09, 2015 6:07 AM To: Histonet Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx’s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide and cassette labelers
Hi Jim, I do not have experience with the Tissue Tex Smartwrite, but I have used the Leica and Thermoscientific printers. Of the two, I prefer the Leica brand cassette and slide printers. The barcodes and human readable are crystal clear. Hope this helps. Kim Kim Shevlin, HT ASCP Histology and Pathology Operations Manager Seventh Wave Laboratories, LLC 743 Spirit 40 Park Drive, Suite 108 Chesterfield, MO 63005 kshev...@7thwavelabs.commailto:kshev...@7thwavelabs.com www.7thwavelabs.comhttp://www.7thwavelabs.com 636.519.4885 (Main Phone) 636.519.4776 (Histology Lab) 618.570.9383 (Cell Phone) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automatic H E slide stainer recommendations
I agree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histot...@imagesbyhopper.com [histot...@imagesbyhopper.com] Sent: Friday, January 09, 2015 3:37 AM To: ian bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Automatic H E slide stainer recommendations I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard ian.bern...@comcast.net wrote: The Leica Autostainer XL has proven effective for our lab. IRB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automatic H E slide stainer recommendations
Agree -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, January 09, 2015 6:37 AM To: ian bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Automatic H E slide stainer recommendations I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard ian.bern...@comcast.net wrote: The Leica Autostainer XL has proven effective for our lab. IRB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Anyone using Biogenex stainers
Hi Everyone, Our lab has just inherited an old Leica cryostat (2800 Frigocut-E), which uses the disc style specimen stages (no post). I've contacted Leica, but they no longer sell them, and the person I spoke to suggested that I contact this list for ideas. Thanks in advance for any and all help! Wendy Wendy L Roberts Research Analyst, Caron Lab Department of Cell Biology Duke University Medical Center Box 3287 DUMC Durham NC 27710 wendy.robe...@dm.duke.edu 919.681.4549 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Aperio
Does anyone have experience setting up a report template for a breast panel on the Aperio? We just got ours set up and haven't had training yet. The thing has been here since November but with holidays and schedules not working it has been a trial. Are trying to get a jumpstart before the pathologists start training and think it should be ready to go immediately. Thanks. Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone Tissue in plastic embedding
Dear Histonetter's, Happy new year to everyone! I come today with a couple of questions regarding human bone embedded in MMA: 1. What is a good technique to dissolve the MMA from the tissue section? Also any suggestions on how to stain with HE? 2. After mounting my tissue sections (5-10microns) with Haupts adhesive, I noticed that the bone within the section was appearing opaque/darkened on the microscope? Any suggestions on what this might be? Thank you very much, -Jesse H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] And other crazy stuff.
Orangutan testicle macro section and alligator jawbones, not my best work, very humbling, after 52 years in the business. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] small biopsy protocol
Hi Akemi – For what it is worth. We used to cut small GI biopsies at 2 levels, 4 sections per level, per slide for HE, plus one extra unstained between levels. We changed last year to 3 levels, 2 sections per level, all mounted on the same slide, plus one extra unstained with 2 sections from between levels 2 and 3 on gastrics for possible H.Pylori stain. For me, this is better because we are getting fewer requests for recuts. I Hope this helps Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Date: Fri, 9 Jan 2015 06:07:05 -0800 From: Eileen Akemi Allison akemiat3...@gmail.com Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx’s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAS rx
Some PAS procedures call for sulphurous rinses after the Schiffs reagent. From your experience is there a noticeable difference if you do not do the rinse? Would it matter if you used sodium metabisulfite rather than potassium metabisulfite? Thank, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automatic H E slide stainer recommendations
I agree as well. The XL is a wokhorse. We have the transfer station and the CV5030 to go with it. Bernice Frederick (HTL) ASCP Senior Research Tech Pathology Core Facility Robert H. Lurie Cancer Center Northwestern University 710 North Fairbanks Court Olson Room 8421 Chicago, IL 60611 312-503-3723 b-freder...@northwestern.edu From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histot...@imagesbyhopper.com [histot...@imagesbyhopper.com] Sent: Friday, January 09, 2015 5:37 AM To: ian bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Automatic H E slide stainer recommendations I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard ian.bern...@comcast.net wrote: The Leica Autostainer XL has proven effective for our lab. IRB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] And other crazy stuff.
You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, I work in a research facility where I do everything. One day a botanist emailed me to cut pine on the cryostat and asked what the largest size should be. I told him 2 mm x 2mm and explained the process as he had no idea what I was going to do. I thought ok, what is pine? He brought 5 pieces of wood pieces (pine) and asked if they could be cut cross section at 10um with 5 sections per slide. Of course this is not something I have ever had expience in doing, and they are hard as a rock. The trick that ended up working, was to soak the wood in warm water for 30 minutes, embed in OCT and cut. After cutting the sections were all balled up, so I floated them on water and picked them up on the slide. The sections were still folded and looked horiffic in my mind but he loved them. He said was going to look at them under a Raman confocal and wanted to see the plant cell wall. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell powell...@mercer.edu wrote: Orangutan testicle macro section and alligator jawbones, not my best work, very humbling, after 52 years in the business. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] And other crazy stuff.
I forgot to mention: dirt Happy Friday Paula On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell powell...@mercer.edu wrote: Orangutan testicle macro section and alligator jawbones, not my best work, very humbling, after 52 years in the business. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Levels for Gastic and Esophogeal Bx's
Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx’s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Levels for Gastic and Esophogeal Bx's
We do 3 levels with no more than 2 sections per level. It is what the pathologists prefer and therefore what we do. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Friday, January 09, 2015 8:07 AM To: Histonet Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx’s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: And other crazy stuff. RE: [Histonet] cutting honey bees
Shrew mandible, spittle bugs, white fly salivary glands, mosquito ovaries. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Paula Sicurello [pat...@gmail.com] Sent: Thursday, January 08, 2015 1:43 PM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Roberta Horner; Douglas Gregg Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees You asked for it: squid tentacle tip, frog oocyte, serial sections through a entire zebra fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky little boogers), probably lots of others that I can't remember off the top of my head. Paula On Thu, Jan 8, 2015 at 3:20 PM, Michael Ann Jones mjo...@metropath.com wrote: We did a goldfish once, interesting microscopically and difficult for peeling (lots of keratin?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 1/6/15, 12:23 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tesr
Please disregard Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Automatic H E slide stainer recommendations
I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard ian.bern...@comcast.net wrote: The Leica Autostainer XL has proven effective for our lab. IRB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Levels for Gastic and Esophogeal Bx's
Same here Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Friday, January 09, 2015 9:34 AM To: 'Eileen Akemi Allison'; Histonet Subject: RE: [Histonet] Levels for Gastic and Esophogeal Bx's We do 3 levels with no more than 2 sections per level. It is what the pathologists prefer and therefore what we do. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Friday, January 09, 2015 8:07 AM To: Histonet Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx’s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
