[Histonet] Unprocessing tissue

2016-09-23 Thread Armstrong, Karoleigh T via Histonet
Hi Caroline, This may sound a little crazy, but I was working on some mummified tissue back in the 80's, (Mommy is old), and to deal with the dried out tissue we would give them, 1. A quick .5 percent Sodium Hydroxide soak, 5minutes 2. Wash is running water, 1 minute 3. Soak in a 3 parts

[Histonet] FW: Coplin Jar cleaning

2016-09-23 Thread Romundstad, Pamela K via Histonet
Hi, Does anyone have a chirp proof way of cleaning coplin jars? Does anyone store special stain dyes and solutions in coplin jars other than glass? If so, can you share with me your cleaning protocol and which coplin jars you use? We've had too many issues in our lab and washroom with

[Histonet] Subject: CAP Inspection

2016-09-23 Thread T H via Histonet
Hey Banjo, Yes, I have been inspected without any real representative from histology. My current employer sends a Pathologist, PhD and the AP Manager (Cytotechnologist with no real Histology experience) to our CAP inspection. The person really doesn't need to know anything about Histology

Re: [Histonet] Question on Sanderson Rapid Bone Stain

2016-09-23 Thread Jessica Riggleman via Histonet
RBS is the same as Methylene Blue, I believe. _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax:

Re: [Histonet] Microm HM 355S Issues

2016-09-23 Thread Jay Lundgren via Histonet
With most microtomes, 1 complete turn of the wheel equals one advance of four microns, or whatever you have the micrometer set on. What people don't realize with the HM355 is that is doesn't take a complete turn of the fine advance wheel to advance the specimen. If I recall correctly, the

Re: [Histonet] siphon drum pump

2016-09-23 Thread Jessica Riggleman via Histonet
Lauren, What is your pump made out of? _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax:

Re: [Histonet] Microm HM 355S Issues

2016-09-23 Thread Duddey, Aimee via Histonet
We are currently having the same problem with 2 of ours. Stepper motor replacement did not work either. I would be interested to hear any responses as well. Aimee -Original Message- From: Sandra Cheasty via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, September

[Histonet] Microm HM 355S Issues

2016-09-23 Thread Sandra Cheasty via Histonet
Hello, We have 2 Microm HM355S motorized microtomes. We use the motor to move the block close to the blade, but turn the wheel manually when sectioning. Both are "jumping" forward on their own occasionally while sectioning, which is unsettling and chunks into the block. One of

[Histonet] siphon drum pump

2016-09-23 Thread Lauren Sweeney via Histonet
Good morning and happy Friday everyone! I am looking to buy some siphon drum pumps- does anyone out there use them? We have three and they are all leaking at the squeeze/bulb area from the xylene breaking it down. Does anyone know if they manufacture some that are coated to protect against

[Histonet] Adipose Tissue on Frozen Sections

2016-09-23 Thread Meilus, Sheri D. via Histonet
Hello Netters, Does anyone have a protocol for optimizing section quality for specimens with considerable adipose tissue to produce cryostat sections? We have had several instances recently with derm specimens with considerable subcutaneous adipose tissue as well as lymph nodes with