Re: [Histonet] aniline oil/Holzer stain

[Bryan Llewellyn via Histonet]

Aniline oil is more commonly known just as aniline. Sigma list it for 
sale at: 
http://www.sigmaaldrich.com/catalog/search?term=aniline=All=0=match%20partialmax=en=CA=product


Bryan Llewellyn



Susan Bachus via Histonet wrote:

I'm trying to locate aniline oil for the Holzer glial fiber stain.  I
purchased what was listed as aniline oil from ENG, but the first hint
that something was wrong was that it was incompatible with the
chloroform also included in the differentiating solution.  Looking at
the fine print on the bottle, I saw that it was actually "aniline oil,
2.5% aqueous", which I would have interpreted as only containing 2.5%
water, except that it seemed to be completely aqueous (2.5% aniline?),
inasmuch as it wouldn't mix with the chloroform.  I can't find any other
source online.  I see that it is also called aminobenzine or
phenylamine, but am also having trouble locating sources under those
names.  I realize aniline oil is relatively toxic, but am using it under
a fume hood.  I tried dipping slides separately in the (apparently
aqueous) aniline and then chloroform, but still didn't get good
differentiation.  Can anyone suggest a source or a substitute?  Many
thanks for your help!   Susan

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Re: [Histonet] aniline oil/Holzer stain

[Paula Keene Pierce via Histonet]

You could try adding aqueous NaOH slowly to what you have until you start 
getting tiny white droplets in the water. Then they will fall and form an oily 
layer below the water.
Decant the water or use a separator funnel to drain the aniline oil out the 
bottom.

 Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com

  From: Susan Bachus via Histonet 
 To: histonet@lists.utsouthwestern.edu 
 Sent: Friday, October 21, 2016 5:28 PM
 Subject: [Histonet] aniline oil/Holzer stain
   
I'm trying to locate aniline oil for the Holzer glial fiber stain.  I 
purchased what was listed as aniline oil from ENG, but the first hint that 
something was wrong was that it was incompatible with the chloroform also 
included in the differentiating solution.  Looking at the fine print on the 
bottle, I saw that it was actually "aniline oil, 2.5% aqueous", which I 
would have interpreted as only containing 2.5% water, except that it seemed 
to be completely aqueous (2.5% aniline?), inasmuch as it wouldn't mix with 
the chloroform.  I can't find any other source online.  I see that it is 
also called aminobenzine or phenylamine, but am also having trouble locating 
sources under those names.  I realize aniline oil is relatively toxic, but 
am using it under a fume hood.  I tried dipping slides separately in the 
(apparently aqueous) aniline and then chloroform, but still didn't get good 
differentiation.  Can anyone suggest a source or a substitute?  Many thanks 
for your help!  Susan 


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[Histonet] aniline oil/Holzer stain

[Susan Bachus via Histonet]

I'm trying to locate aniline oil for the Holzer glial fiber stain.  I 
purchased what was listed as aniline oil from ENG, but the first hint that 
something was wrong was that it was incompatible with the chloroform also 
included in the differentiating solution.  Looking at the fine print on the 
bottle, I saw that it was actually "aniline oil, 2.5% aqueous", which I 
would have interpreted as only containing 2.5% water, except that it seemed 
to be completely aqueous (2.5% aniline?), inasmuch as it wouldn't mix with 
the chloroform.  I can't find any other source online.  I see that it is 
also called aminobenzine or phenylamine, but am also having trouble locating 
sources under those names.  I realize aniline oil is relatively toxic, but 
am using it under a fume hood.  I tried dipping slides separately in the 
(apparently aqueous) aniline and then chloroform, but still didn't get good 
differentiation.  Can anyone suggest a source or a substitute?  Many thanks 
for your help!   Susan 



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Re: [Histonet] Plant tissue

[Loralei Dewe via Histonet]

You should contact David Carter at UC Riverside. It is his area of
expertise!

Loralei

On Oct 21, 2016 12:55 PM, "Caroline Miller via Histonet"  wrote:

> I am interested in this too! Please forward to all. What are the best
> resources for how to handle plant material for microscopic analysis?
>
> thanks!
> mills
>
> On Fri, Oct 21, 2016 at 12:28 PM, John Shelley via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> > Happy Friday!
> >
> > I have given a request for service to me to do some plant tissue
> histology
> > and I must admit this is a new area for me. I plan to do both paraffin
> and
> > frozen sectioning and would like to ask if there may be anyone has a
> > protocol on how to handle such tissue in regards to a tissue processing
> > schedule and also steps to take for freezing. Any information would be
> > greatly appreciated.
> >
> > Have a great weekend!
> >
> > Kind Regards!
> >
> > John J Shelley
> > Histology Core Manager
> > Sanford Burnham Prebys Medical Discovery Institute at Lake Nona
> >
> > ___
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> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --
> Caroline Miller (mills)
> Director of Histology
> 3Scan.com
> 415 2187297
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Re: [Histonet] Plant tissue

[Caroline Miller via Histonet]

I am interested in this too! Please forward to all. What are the best
resources for how to handle plant material for microscopic analysis?

thanks!
mills

On Fri, Oct 21, 2016 at 12:28 PM, John Shelley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Happy Friday!
>
> I have given a request for service to me to do some plant tissue histology
> and I must admit this is a new area for me. I plan to do both paraffin and
> frozen sectioning and would like to ask if there may be anyone has a
> protocol on how to handle such tissue in regards to a tissue processing
> schedule and also steps to take for freezing. Any information would be
> greatly appreciated.
>
> Have a great weekend!
>
> Kind Regards!
>
> John J Shelley
> Histology Core Manager
> Sanford Burnham Prebys Medical Discovery Institute at Lake Nona
>
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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[Histonet] Plant tissue

[John Shelley via Histonet]

Happy Friday!

I have given a request for service to me to do some plant tissue histology and 
I must admit this is a new area for me. I plan to do both paraffin and frozen 
sectioning and would like to ask if there may be anyone has a protocol on how 
to handle such tissue in regards to a tissue processing schedule and also steps 
to take for freezing. Any information would be greatly appreciated.

Have a great weekend!

Kind Regards!

John J Shelley
Histology Core Manager
Sanford Burnham Prebys Medical Discovery Institute at Lake Nona

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[Histonet] Tissue Processor question

[Terri Braud via Histonet]

Hi fellow Histonetters-
I'm looking for any feedback from working labs using Thermo's Excelsior 
Processor.  If you can help, can you please also include the age and usage on 
your instrument?
Thanks, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874

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[Histonet] Part 3 - Book and Atlases they're going fast

[Connolly, Brett M via Histonet]

Hi all,

Thanks for all of your responses!  The list has shrunk considerably.

Here is what left.  I forgot to add the Sheehan & Hrapchak classic: Theory and 
Practice of Histotechnology (this one is a little worn---go figure).

Atlas of UroSurgical Anatomy;   ISBN 0-7216-3955-0  $30.00
Comparative Anatomy and Histology: A Mouse and Human atlas;   ISBN 
978-0-12-381361-9  $80.00
Human Histology 2nd Ed;   ISBN 0-7234-2485-3  $40.00
Immunochemistry (LabFax);  ISBN 1-872748-05-8   $12.00
Atlas of Normal Histology 6th Ed;   ISBN 0-8121-1126-5   $12.00
Primate Brain Maps: Structure of the Macaque Brain;   ISBN 0-444-50415-X   
$50:00
Structure of the Human Brain: A Photographic Atlas 3rd Ed:  ISBN 0-19-504357-X  
 $20.00
Theory and Practice of Histotechnology 2nd Ed;   ISBN 0-8016-4573-5  $50.00

Free books
A Textbook of Histology 10th Ed;   ISBN 0-7216-1757-3
The Cell: Its Organelles and Inclusions - Atlas of Fine Structure;  ISBN 
0-7216-3585-7
Ham' s Histology 9th Ed;  ISBN0-397-50681-3
Basic Histology 6th Ed:   ISBN 0-8385-0575-9
The Human Brain: An introduction to Its Functional Anatomy 5th Ed;  
ISBN0-323-01320-1
Animal Processing Manual 1st Ed; Nat. Soc for Histotechnology
Dictionary of Human Neuroanatomy   ISBN 3-540-66523-4



Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

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Re: [Histonet] TGIF - Help me with my mental block! The "PA" dilemma...

[Jay Lundgren via Histonet]

pathologists'

On Fri, Oct 21, 2016 at 6:56 AM, Pam Barker via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Histonetters!
> This might seem like a dumb question but every time I get a position for a
> PA it seems that I can never remember if it should be pathologists'
> assistant or pathologist's assistant or pathologists assistant.
> Can somebody please clarify for me?
> And yes as you probably already guessed I am working on a PA position right
> now.  It is in Tennessee.  If anyone is interested please shoot me an email
> or give me a call for more details.
>
> I really need the answer to the question though.
> Thanks to all - have a great weekend!!
>
>
> Thanks-Pam
>
> Right Place, Right Time, Right Move with RELIA!
>
> Thank You!
>  Pam M. Barker
>
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: rel...@earthlink.net
> https://www.facebook.com/RELIASolutionsforhistologyprofessionals
> www.facebook.com  /PamBarkerRELIA
> www.linkedin.com/in/reliasolutions
> www.twitter.com/pamatrelia
>
>
>
>
>
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[Histonet] TGIF - Help me with my mental block! The "PA" dilemma...

[Pam Barker via Histonet]

Hi Histonetters!
This might seem like a dumb question but every time I get a position for a
PA it seems that I can never remember if it should be pathologists'
assistant or pathologist's assistant or pathologists assistant.
Can somebody please clarify for me?
And yes as you probably already guessed I am working on a PA position right
now.  It is in Tennessee.  If anyone is interested please shoot me an email
or give me a call for more details.

I really need the answer to the question though.
Thanks to all - have a great weekend!!


Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





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Re: [Histonet] JC requirement for stain QC question

[Cates, Julia via Histonet]

Hi Tim,

We have a form here that was developed after a similar inspection.  The form 
has quality control indicators for both the histologist and pathologist to 
review.  At the end of the day, the pathologist will initial as reviewed for 
the entire form.  He also documents QC in the patients report for Specials and 
IHC controls.

Thanks,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Park Ridge Health
(828)650-8243| Fax: (828)209-5315

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