[Histonet] Label-less slides

[Val Tolley via Histonet]

Hi All-

Does anyone know where I can buy charged slides without a frosted end for a 
label?  All I can find is plain, uncharged.
...Or alternatively, a charged slide with a shortened frosted edge allowing 
more room at the top of the slide for tissue adhesion.

Val


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Re: [Histonet] TUNEL

[Caroline Miller via Histonet]

I have done that a lot and I don't see why it would not pick up hx or DAPI.
Was there anything left on the slide? Maybe it was mounted in aqueous
already??

mills

On Fri, Mar 3, 2017 at 11:42 AM, Bernice Frederick via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello all.
> Would there be any reason that slides stained with TUNEL (ISH) would not
> pick up the Mayers counterstain? We use it for IHC and it is fine. Mayers
> for 7 minutes and then ammonia water for 1 minute. We do that rather than
> rinse for 15 minutes and there are usually no issues. Could a reagent in
> the stain be causing it? DAPI is used...
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-freder...@northwestern.edu
>
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] query

[Monson, Frederick via Histonet]

Have no expertise other with 'library.'
So, here is some new stuff that I found.

http://www.autoqbiosciences.com/products/3d-laser-nanodissection-systems/tissue-surgeon/
 

http://www.autoqbiosciences.com/_webedit/uploaded-files/All%20Files/Nano/LLS-Brosch%25C3%25BCre_TissueSurgeon_HardTissue_2014-03-18_web.pdf
 

There are lots of methods, but few that can be followed with microdissection.

Cheers,

Fred Monson


Frederick  C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of PA
Geology-Astronomy
750 South Church St.
West Chester, PA, 19383
fmon...@wcupa.edu
610-738-0437(Work)




-Original Message-
From: Nair, Indu via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 03, 2017 1:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] query

Is there a protocol to section un-decalcified bone? Or is there a laboratory 
that does this routinely?
thank you
indu


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[Histonet] TUNEL

[Bernice Frederick via Histonet]

Hello all.
Would there be any reason that slides stained with TUNEL (ISH) would not pick 
up the Mayers counterstain? We use it for IHC and it is fine. Mayers for 7 
minutes and then ammonia water for 1 minute. We do that rather than rinse for 
15 minutes and there are usually no issues. Could a reagent in the stain be 
causing it? DAPI is used...
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

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[Histonet] VIPE300

[Paula via Histonet]

Hello,

We're looking at purchasing a refurbished VIP E300 and I was hoping for some
feedback on this processor. Is it reliable, dependable, good workmanship
kind of machine or are they nothing but trouble?

Thanks for any replies and have a good day.

Paula

 

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[Histonet] Day Shift Histotech in Buffalo

[Angie Laparidis via Histonet]

TGIF Histonet subscribers!

I wanted to fill you all in on a brand new DAY SHIFT position in the
Buffalo, NY area! This location is fantastic for those who value all four
seasons! Day shift opportunities are hard to come by, and this position
will not be open very long.

Take a look at the area (http://www.visitbuffaloniagara.com/) and send over
a resume so we can further discuss your next career move!


Look forward to hearing from you!


Sincerely,


*Angie Laparidis*Healthcare Recruiter
K.A. Recruiting, Inc.
10 Post Office Square, 8th Floor South, Boston, MA, 02109
W:  617.746-2744 (*please note this is a new number*)
F: (617) 507-8009
an...@ka-recruiting.com


Our openings are updated daily at www.ka-recruiting.com
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[Histonet] query

[Nair, Indu via Histonet]

Is there a protocol to section un-decalcified bone? Or is there a laboratory
that does this routinely?
thank you
indu


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the intended recipient may be able to view the information, forward it to 
others or tamper with the information without the knowledge or consent of the 
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wish to r
 eceive further communications via e-mail, please reply to this message and 
inform the sender that you do not wish to receive further e-mail from the 
sender. (LCP301)
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[Histonet] Looking for control tissue

[Altemus, Christine via Histonet]

Hi - We are currently in need of Lung Adenocarcinoma and Lung Squamous Cell 
carcinoma tissue to use for controls for Pulmo Panel IHC Staining. Stains 
include Desmoglein 3 + Napsin A cocktail, TTF-1 + CK5 cocktail and p63+TRIM29 
Cocktail. If anyone has some that they can spare, kindly pm me. Thanks in 
advance for any assistance you can provide.

Chrissy - St. Mary's Healthcare, Amsterdam-NY


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[Histonet] RELIA Hot Histology Job Alert!! Live and work in Beautiful Coastal North Carolina with this RELIA exclusive!!!

[Pam Barker via Histonet]

Hi Histonetters!!
TGIF!!!
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This is a full time permanent position and my client is looking for an ASCP
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You can also reach me toll free at the office at 866-607-3542 or via email
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If you know someone who might be interested and you refer them to me you
will earn a referral fee if I place them.  That means if they get placed in
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Have a great day and a great weekend!!  Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
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Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
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Phone: (407)657-2027
Cell: (407)353-5070
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www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
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