Re: [Histonet] Histonet Digest, Vol 167, Issue 12

[Haley Huggins via Histonet]

My suggestion for using the Dako IHC manual is not necessarily just about
the automated application, but about the antibodies themselves and what the
end results are supposed to be. It isn't the only thing you should use to
prepare for the QIHC exam, but if you want to learn about a large amount
of antibodies and what they target, the Dako IHC manual is the book of
choice. I have a friend that took the exam a few years back and that was
her suggestion as well. Yes you should know about the different platforms,
but the use of the antibodies in IHC staining will still be used to get the
targeted results provided in the Dako manual. The other platform manuals do
not provide the same detailed descriptions of the antibodies and their
purposes as the Dako manual does. In my lab, we run our IHC by hand, so I
don't use any of the automated platforms that are available today, but
luckily I know how to use most if not all of them from previous employment.
Pull from your experiences with IHC, read up from all the platforms, use
the Dako manual as a guide for a large variety of the antibodies, and check
out the study guide from Michigan Society of HIstotechnology. If you are
lucky attend a seminar on preparing for the exam. I have taken a couple of
them, they can be helpful.

Haley

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*

On Tue, Oct 17, 2017 at 3:00 PM, Sally Price via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> All:
> I beg to differ with what seems to be the general consensus, but the Dako
> handbook is not really representative of the available technology within
> the IHC field and shouldn't be thought of as a prescription for
> success.  All the Dako book addresses is Dako's methods, reagents and
> instruments, which is pretty biased.  In order to properly prepare for the
> ASCPs exam, one needs to think beyond reagents and automated systems and
> REALLY understand the underlying science.  I've attended a number of
> seminars on this subject and learned that, especially because of recent
> changes to this test, one also needs to understand the clinical application
> of these procedures.  More and more we're called upon to provide
> pathologists with support in the way of procedure validation and
> troubleshooting and we need to learn a great deal more than can be learned
> from reading one vendor's publication.
> +Sally
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Re: [Histonet] Histonet Digest, Vol 167, Issue 12

[Sally Price via Histonet]

All:
I beg to differ with what seems to be the general consensus, but the Dako
handbook is not really representative of the available technology within
the IHC field and shouldn't be thought of as a prescription for
success.  All the Dako book addresses is Dako's methods, reagents and
instruments, which is pretty biased.  In order to properly prepare for the
ASCPs exam, one needs to think beyond reagents and automated systems and
REALLY understand the underlying science.  I've attended a number of
seminars on this subject and learned that, especially because of recent
changes to this test, one also needs to understand the clinical application
of these procedures.  More and more we're called upon to provide
pathologists with support in the way of procedure validation and
troubleshooting and we need to learn a great deal more than can be learned
from reading one vendor's publication.
+Sally
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[Histonet] ICYMI: Check it out my second "Make the Cut" column on Interviewing Tips for Histologists in the NSH Fall 2017 Career Center Newsletter! 10/17/2017

[Pam Barker via Histonet]

Hi Histonetters,

ICYMI - In case you missed it!!

I am so excited!!  My Second article has been published in the NSH Quarterly 
Career Center Newsletter under my new byline:
 
Make the Cut
 
I will be writing for the NSH Career newsletter on a quarterly basis and a few 
months ago in one of my emails I asked for topics of interest in anticipation 
of this column.  Thanks again for the suggestions and please keep them coming!!
 
The first column was about Salary Negotiations and the second one is 
Interviewing Tips for Histologists.
Here is the link to both articles: 

http://nsh.org/content/career-center-newsletter
 
If you have a minute to read it I would love to hear what you think.
 
I also have some exciting job opportunities to pass along.
One of these could give you the chance to try out those interviewing tips for 
histologists!! ☺
Please take a look and if you are interested let me know.  If you have a friend 
who is interested and I place them then I get to give you a referral reward! 
 
I LOVE TO GIVE REFERRAL REWARDS!!
 
SOME of these are RELIA exclusives…
MOST of these offer Sign- on Bonuses and/or Relocation Assistance
ALL of these Companies offer excellent compensation, benefits and great 
environments, opportunity for growth and great people to work with.  
AND THEY ALL ARE READY TO HIRE!!
 
What more could you ask for?
 
If You Or Anyone You Know Might Be Interested In A New Opportunity  Please 
Contact Me.

If you want to chat ASAP call or text me on my cell 
at 407-353-5070.
If you want some additional information or to set up a time to chat please call 
me toll free at 866-607-3542 or email me at rel...@earthlink.net 

Here are some of our Hottest Opportunities:
 
We have leadership opportunities in:
Florida, Virginia and North Carolina
 
We have Histotechnician/Histotechnologist opportunities in:
California, Texas, Tennessee, New York and North Carolina

We have IHC Specialist Positions in:
North Carolina and Massachusetts 
 
And my phone is still ringing off the hook so if you don’t see the location you 
want yet drop me a line so I can let you know when something DOES come up.  I 
could be talking to a client about your next opportunity RIGHT NOW!!
 
Have a great day.  I look forward to hearing back from you.
 
Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





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Re: [Histonet] QIHC Study Materials

[Haley Huggins via Histonet]

I sent to Lindsey in a private email which I thought I had hit reply all
that Michigan Society of Histotechnology also has a study guide for the
IHC, but you are right, Jay, that the Dako IHC manual is your best study
guide.

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*

On Tue, Oct 17, 2017 at 2:32 AM, Jay Lundgren via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> The free one from Dako.
>
>  utm_source=link_campaign=sig-email_content=webmail>
> Virus-free.
> www.avg.com
>  utm_source=link_campaign=sig-email_content=webmail>
> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>
>
> On Mon, Oct 16, 2017 at 12:28 PM, Fairbourn, Lindsey S. via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> > Hello Histonet,
> >
> > What is the best textbook to use to study for the QIHC exam?
> >
> > Thanks for your help!
> > Lindsey
> >
> >  ---
> > The information transmitted by this e-mail and any included
> > attachments are from ARUP Laboratories and are intended only for the
> > recipient. The information contained in this message is confidential
> > and may constitute inside or non-public information under
> > international, federal, or state securities laws, or protected health
> > information and is intended only for the use of the recipient.
> > Unauthorized forwarding, printing, copying, distributing, or use of
> > such information is strictly prohibited and may be unlawful. If you
> > are not the intended recipient, please promptly delete this e-mail
> > and notify the sender of the delivery error or you may call ARUP
> > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1
> > (800) 522-2787 ext. 2100
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[Histonet] Qiagen Qproteome FFPE Tissue Kit

[Kalleberg, Kristopher via Histonet]

Hello,

Has anyone tried the Qproteome FFPE Tissue Kit kit from Qiagen:

Qproteome FFPE Tissue Kit
For isolation of full-length proteins from formalin-fixed tissues
* Standardized, quantitative extraction from FFPE tissue
* Full-length proteins
* Low sample requirements
The Qproteome FFPE Tissue Kit uses innovative proprietary chemistry to reverse 
the crosslinking process of formalin fixation and deliver full-length proteins 
ready for subsequent analysis.
Just curious if these kits are truly able to allow for isolation of proteins 
from formalin fixed tissue.  I know this technology has been used for a number 
of years manually, just wondering how these kits are to manual methods and if 
it can be used for all proteins or only a certain proteins or sizes.

Thank you in advance.

Kris

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[Histonet] coverslipper Dako question

[Montse Verdú via Histonet]

Dear histonetters,



Does anyone experience with the Coverslipper Dako?



How often do you experience problems with these instruments that you need
to

have service for them? What kind of problems are they?



In our case the drawer is not securely attached, the magnet is not securely
attached to the equipment so the basket collides with the drawer and breaks
the slides.

Does anyone else have this same problem?



Thank you in advance!



Montse Verdú

Histopat laboratoris

Tel +34932033000

Biopat. Biopatologia Molecular

Tel+34932030837

mve...@histopat.es



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[Histonet] RV: Tissue-Tek VIP 6 AI Vacuum Infiltration Processor

[Montse Verdú via Histonet]

Dear histonetters,



Does anyone experience with the Tissue-Tek VIP 6 AI vacuum infiltration
procesor?

How often do you experience problems with these instruments that you need
to

have service for them? What kind of problems are they?



Thank you in advance!



Montse Verdú

Histopat laboratoris

Tel +34932033000

Biopat. Biopatologia Molecular

Tel+34932030837

mve...@histopat.es



Aquest missatge i els seus annexos estan adreçats exclusivament a la
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informació confidencial, sotmeses a secret professional o la divulgació de
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aquest missatge per error, si-us-plau, contacti amb la persona que figura
com a remitent i procedeixi a la seva eliminació.



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recibido este mensaje por error, por favor, contacte con la persona que
figura como remitente y proceda a su eliminación.



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[Histonet] Tissue-Tek VIP 6 AI Vacuum Infiltration Processor

[Montse Verdú via Histonet]

Dear histonetters,



Does anyone experience with de Tissue-Tek VIP 6 AI vacuum infiltration
procesor?

How often do you experience problems with these instruments that you need
to

have service for them? What kind of problems are they?



Thank you in advance!



Montse Verdú

Histopat laboratoris

Tel +34932033000

Biopat. Biopatologia Molecular

Tel+34932030837

mve...@histopat.es



Aquest missatge i els seus annexos estan adreçats exclusivament a la
persona o entitat que figura com a destinatari i poden contenir dades i/o
informació confidencial, sotmeses a secret professional o la divulgació de
les quals estigui prohibida en virtut de la legislació vigent. Tota
distribució, divulgació o reproducció de aquesta comunicació per part de
persones o entitats diferents del destinatari està prohibida. Si ha rebut
aquest missatge per error, si-us-plau, contacti amb la persona que figura
com a remitent i procedeixi a la seva eliminació.



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Re: [Histonet] QIHC Study Materials

[Jay Lundgren via Histonet]

The free one from Dako.


Virus-free.
www.avg.com

<#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>

On Mon, Oct 16, 2017 at 12:28 PM, Fairbourn, Lindsey S. via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello Histonet,
>
> What is the best textbook to use to study for the QIHC exam?
>
> Thanks for your help!
> Lindsey
>
>  ---
> The information transmitted by this e-mail and any included
> attachments are from ARUP Laboratories and are intended only for the
> recipient. The information contained in this message is confidential
> and may constitute inside or non-public information under
> international, federal, or state securities laws, or protected health
> information and is intended only for the use of the recipient.
> Unauthorized forwarding, printing, copying, distributing, or use of
> such information is strictly prohibited and may be unlawful. If you
> are not the intended recipient, please promptly delete this e-mail
> and notify the sender of the delivery error or you may call ARUP
> Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1
> (800) 522-2787 ext. 2100
> ___
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>
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