Charles, What do you mean by "dark nuclei"? Are you asking about the normal
colour for the method, or about something you have not seen before that looks
wrong? Also, what is "PAP stain"?
If "PAP stain" means Papanicoloau, the nuclear stain is Mayer's haemalum. This
is a progressive stain;
We have a Histotech position open in a GI lab located outside Dayton Ohio. This
position is Monday - Friday day shift position. We are a growing company that
has just opened a brand new facility, with windows so you can actually see
outside! You must be HT (ASCP) certified or eligible and
There are many reasons why the nuclei may look dark in the Pap stain.
1. What type of hematoxylin are you using?
2. What type of Pap stain are you using, regressive or progressive?
This could be due to a variety of things like: type of preparation utilized,
too long in hematoxylin, not enough
What causes dark nuclei in the PAP stain.
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
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Hi Histonetters!
I Hope You Had a Fun and Lucky St. Patricks Day.
And A Happy Histotechnology Professionals Day.
(Did your lab do anything special?)
March Madness has started and in addition to some great College Basketball.
I have been experiencing the madness as well.
My phone has