[Histonet] Mini Lab Incubators?

[Cooper, Brian via Histonet]

Good Afternoon Histonetters!

Does anyone out there have any recommendations as far as Mini Lab Incubators 
go?  Have looked at the VWR Digital Mini Incubator and LabNet International 
Mini Incubator online this morning.  Haven't seen much in the way of comments 
on these products; would love to hear your feedback.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] FISH for breast core (Her2)

[Mark Tarango via Histonet]

 Hello Taganrog,

If you are having issues with high autoflorescence due to prolonged
formalin fixation (7 days), it might help to do a few minutes extra
protease digestion (5+ minutes) during tissue pretreatment.  Alternately or
in conjunction, I would suggest re-applying HER2 probe to the same slide
that had already been pretreated and hybridized.  By doing this a few times
(even up to four tries), you will likely get FISH signals that you can see
and enumerate.

Good luck,

Mark Tarango

On Tue, Jun 11, 2019 at 11:33 AM Пешков Максим via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
> Dear colleagues!
> Can anyone help? We are very appreciatated any suggestions, tips and hacks
> for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more
> than 7 days, then processed as usual into wax. IHC for Her2 has value as 2+.
> Sincerely,
> Maxim Peshkov,
> Russia,
> Taganrog.
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Re: [Histonet] FISH for breast core (Her2)

[John Garratt via Histonet]

Maxim, this is a link to the cIQc assessment page 
http://cpqa.ca/main/?page_id=24
If you look at the HER2 ISH assessments you will see numerous protocols used by 
laboratories. To my knowledge the protocols work on both biopsy and resection 
tissue.

John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Tuesday, June 11, 2019 11:18 AM, Пешков Максим via Histonet 
 wrote:

>
>
> Dear colleagues!
> Can anyone help? We are very appreciatated any suggestions, tips and hacks 
> for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more than 
> 7 days, then processed as usual into wax. IHC for Her2 has value as 2+.
> Sincerely,
> Maxim Peshkov,
> Russia,
> Taganrog.
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Re: [Histonet] IHC mouse optomization

[Colleen Forster via Histonet]

Biocare Medical Promark series are also great animal polymer based
detection that work very well.

https://biocare.net/products/detection/

Colleen Forster HT(ASCP)WQIUC
U of MN

On Wed, Jun 12, 2019 at 10:42 AM Paula Keene Pierce via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Vector Labs ImmPress kits rock for animal IHC.
> https://vectorlabs.com/browse/immpress-polymer-detection-kits-for-ihc
>
>
>
>
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830
> N Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com
>
> On Wednesday, June 12, 2019, 10:13:48 AM CDT, Blanca Lopez via
> Histonet  wrote:
>
>  Morning,
> I need a good advice in optimizing Mouse antibodies for research purposes.
> I use Dako/Agilent products. COX6B2 from Sigma is giving us hard times.
> Dako kit HRP has a mixture of mouse and rabbit so we think that might be
> the reason become negative.
> Is there any tips for optimizing antibodies special for mouse tissue? Or
> if you can share your best procedures in IHC mouse stains. I can have a
> different ways and products to try. Any suggestion or opinions count. If
> you have any website that I can learn more about IHC for research mainly
> done in  Xenograft.
> Thank you everybody:)
>
> Blanca Lopez HT (ASCP)cm
> Senior Histotechnologist
> UT Southwestern Medical Center
> Harold C. Simmons Comprehensive Cancer Center
> UTSTR Biorepository Tissue Lab
> 6000 Harry Hines Blvd NB5.102
> Dallas, Texas 75390
> 214-648-7598
> blanca.lo...@utsouthwestern.edu
>
>
>
> 
>
> UT Southwestern
>
>
> Medical Center
>
>
>
> The future of medicine, today.
>
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>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB  612-626-1930

*If submitting histology request please also forward to Lori Holm at
ho...@umn.edu *
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Re: [Histonet] IHC mouse optomization

[Paula Keene Pierce via Histonet]

Vector Labs ImmPress kits rock for animal IHC.
https://vectorlabs.com/browse/immpress-polymer-detection-kits-for-ihc




Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com 

On Wednesday, June 12, 2019, 10:13:48 AM CDT, Blanca Lopez via Histonet 
 wrote:  
 
 Morning,
I need a good advice in optimizing Mouse antibodies for research purposes. I 
use Dako/Agilent products. COX6B2 from Sigma is giving us hard times. Dako kit 
HRP has a mixture of mouse and rabbit so we think that might be the reason 
become negative.
Is there any tips for optimizing antibodies special for mouse tissue? Or if you 
can share your best procedures in IHC mouse stains. I can have a different ways 
and products to try. Any suggestion or opinions count. If you have any website 
that I can learn more about IHC for research mainly done in  Xenograft.
Thank you everybody:)

Blanca Lopez HT (ASCP)cm
Senior Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center
UTSTR Biorepository Tissue Lab
6000 Harry Hines Blvd NB5.102
Dallas, Texas 75390
214-648-7598
blanca.lo...@utsouthwestern.edu





UT Southwestern


Medical Center



The future of medicine, today.

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[Histonet] IHC mouse optomization

[Blanca Lopez via Histonet]

Morning,
I need a good advice in optimizing Mouse antibodies for research purposes. I 
use Dako/Agilent products. COX6B2 from Sigma is giving us hard times. Dako kit 
HRP has a mixture of mouse and rabbit so we think that might be the reason 
become negative.
Is there any tips for optimizing antibodies special for mouse tissue? Or if you 
can share your best procedures in IHC mouse stains. I can have a different ways 
and products to try. Any suggestion or opinions count. If you have any website 
that I can learn more about IHC for research mainly done in  Xenograft.
Thank you everybody:)

Blanca Lopez HT (ASCP)cm
Senior Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center
UTSTR Biorepository Tissue Lab
6000 Harry Hines Blvd NB5.102
Dallas, Texas 75390
214-648-7598
blanca.lo...@utsouthwestern.edu





UT Southwestern


Medical Center



The future of medicine, today.

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[Histonet] (no subject)

[Cindy Bird via Histonet]

Can anyone in the Dallas area tell me how they dispose of glass slides and what 
the regulations are?
Cindy Bird
Anatomical Medical Laboratories, Inc.
1600 Scripture Street
Denton, TX  76201
940-384-6210
940-384-6000
Fax 940-565-9588

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