Re: [Histonet] Histonet Digest, Vol 232, Issue 13

2023-03-23 Thread Ewen Sutherland via Histonet 


Re Gunk

Check any of the seals or washers. When they breakdown they usually present 
with this sort of result on slides

Ewen Sutherland
Anatomical and Digital Product Specialist
Bio-Strategy
T: +61 3 9355 3900 / 1800 00 84 53
M: +61 417 460 019
ewen.sutherl...@bio-strategy.com   www.bio-strategy.com

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Subject: Histonet Digest, Vol 232, Issue 13

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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Cooper, Brian via Histonet 
WOW!  I came back from lunch and I have seven responses already!  Everyone said 
the same thing, which was my game plan, take punches from larger samples!  
Happy Friday Eve everyone!   

Thanks so much Histonet!  

Brian

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 11:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations (EXTERNAL EMAIL)

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Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Paula Sicurello via Histonet 
The one thing that bothers me about the preloaded programs is the paraffin 
temperature is set at 65 degrees C.  That's close to 10 degrees higher than the 
melting point of our paraffin.
Does anyone know why the temp. is so high?  To me, a temp that high will cause 
problems with IHC and small biopsies.  
Has anyone changed it?  Or do folks go along with it just because it's already 
there? 
I inherited that temp, to change it now would be pain since it is the one we 
use the most.  If I validated our current Peloris 3, I would have set the wax 
temperatures at 60 degrees.
Sincerely,
Paula Sicurello 

On Thursday, March 23, 2023 at 11:54:44 AM PDT, Cooper, Brian via Histonet 
 wrote:  
 
 Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Thomas Podawiltz via Histonet 
I ordered small punch biopsy kits and made my own small biopsies from remnant 
tissue. Renal biopsy kits worked for making our own needle biopsies. Leica 
already has the protocols that work so part is easy. 


Sent from Yahoo Mail for iPad


On Thursday, March 23, 2023, 3:05 PM, Normington, Lacy via Histonet 
 wrote:

We have purchased several disposable core biopsy instruments of varying gauges 
and take samples of large resection specimens. We also use the same grossing 
tools to take small samples of GI, endo, ecc, ect.

Lacy
 

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 1:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Normington, Lacy via Histonet 
We have purchased several disposable core biopsy instruments of varying gauges 
and take samples of large resection specimens. We also use the same grossing 
tools to take small samples of GI, endo, ecc, ect.

Lacy
 

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 1:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential or 
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Re: [Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Whitaker, Bonnie via Histonet 
Put in two cassettes of resection tissue that are cut to mimic biopsy 
sizes/tissue (colon resection for colon bx, liver resection for liver bx, etc.

Bonnie Whitaker
Ohio State University
AP Operations Director

614-293-8418

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 2:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential or 
legally privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of this original 
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[Histonet] Tissue Processor Schedule Validations

2023-03-23 Thread Cooper, Brian via Histonet 
Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential or 
legally privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
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