Re: [Histonet] Sudanblack B on FFPET

It's true that Sudan black B won't stain ordinary lipids (fat, phospholipids etc) that are absent from paraffin sections. The lipid in lipofuscin is bound to protein strongly enough to resist extraction during passage through all the solvents used in preparing paraffin sections. Churukian's

Re: [Histonet] Sudanblack B on FFPET

Sudan Black staining won’t work on FFPET. The alcohols and xylenes used in the tissue processing dissolves the lipids in the tissue. However, you can used formalin fixed tissue as an alternative if no frozen section is available. Rinse the tissue in distilled water for several minutes, pat dry,

Re: [Histonet] Sudanblack B on FFPET

I would also let the saturated solution stand for a few days. Like Oil Red O, it probably needs time to “mature”. I would also use a frozen section of skin as a control. Regards, Tony Henwood Sydney, Australia From: Bob Richmond via Histonet Sent:

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Re: [Histonet] Sudanblack B on FFPET

> > Gudrun Lang in Austria asks: > >>Has anyone experience with Sudanblack B on paraffin slides for staining [lipofuscin]? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night.

Re: [Histonet] Sudanblack B on FFPET

You probably would get better results with a supersaturated solution of the dye in 60% isopropanol with added dextrin. For details see https://www.urmc.rochester.edu/urmc-labs/pathology/StainsManual/. Click on Table of Contents and follow the links to fined the method. Be sure to use a batch

[Histonet] Sudanblack B on FFPET

Hallo! Has anyone experience with Sudanblack B on paraffin slides for staining lipofuszin? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night. I've made a saturated