Re: [Histonet] Sections falling off

Hi Pablo I have found with bone sections, which I pick up with silanised slided, that if I apply gelatin to the slides just prior to picking up the sections, the sections don't lift. The slides are dipped in the gelatin solution, then the sections are picked up off the surface of the water.

Re: [Histonet] Microtomy of Equine Tendon Blocks

Hi Alan We have had success with horse's hoof using potassium hydroxide prior to processing. After the hoof is fixed for several weeks we leave the hoof for 12 hours sometimes longer in 10% Potassium Hydroxide and then further trim the tissue in. The Potassium Hydroxide has a marked affect

[Histonet] Flatbed microwave oven for microwave staining antigen retrieval

Hi We currently use a 25 year old turntable kitchen microwave oven. This is used for antigen retrieval, silver staining and several other histochemical stains. When I out shopping the other day a salesman introduced me to the flatbed microwave that heats from below and reputedly gives more

[Histonet] Stored frozen fatty muscle tissue

Hi Histonetters I’ve been trying to cut frozen fatty muscle tissue from 2018/2019 stored at -80C. I managed to cut some sections from the same tissue on fresh frozen tissue in 2018. I suspect that storage may contribute to the difficulty to cut this. I have yet touse dry ice to further lower

[Histonet] Sections sticking to tweezers

Hi Ken There is no magic answer to this. Just persistence and experience. Dexterity will improve over time. Gerard ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Frozen marbled muscle failing to cut

Hi I resolved the problem with my earlier post from the 26th of September by cutting the muscle at a lower temperature -18C. The muscle has previously been embedded in OCT immersed in Isopentane and the into liquid nitrogen and stored in a freezer at -80C. The tissue is Wagyu muscle some of