Hi all,
Does anyone have the method for Hirsch-peiffer cresyl violet used for
metachromatic leukodystrophy? On frozen sections.
We are trying to find out if it is different than the usual cresyl violet with
acetic acid method for Nissl bodies.
I've found many references to it, but none that
Blanca, immunofluorescence (IF) is a subset of immunochemistry.
Immunohistochemistry is also a subset of immunochemistry. There is some overlap
between the two.
Immunohistochemistry denotes immunochemistry done on tissue sections
("-histo-" =" tissue"). But we can also use other enzymes to
Tim, I have to agree with Rene that the formalin or time in formalin is
obviously not the problem - it has plenty of time in formalin (and who would
dilute it anyway?). Handling before formalin must always be determined when
problems arise. If the sample sits on a paper towel, gauze etc it does
Something new to me... A pathologist said he is getting reports that LED
illumination on newer microscopes is leading to faint or even false negatives
for congo red amyloid birefringence due to the difference in spectrum between
LED and tungsten filament lamps. Has anyone else noticed this?
Allison, I've never heard of any such requirement. The only requirements have
to do with vapor exposure. If the levels are below the OSHA standards it is ok.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC
Amanda, we use the Fisher Scientific traceable (NISH certificated) for general
hi/lo recording. It just records Hi and Lo over whatever time period until it
is reset. It does not tell you when the hi/lo occurred. You can get a model
that has a 30 day memory and records the time of the hi and lo
Cassandra, for CLIA, CAP and Joint Commission, microtomy and H staining (as
well as embedding and special stains) are classified as "specimen processing"
and do not come under the complexity designation. The reason is that the
histotechs are not doing any "testing" which is defined as
To the specific question of if lab-made predilutes will last longer than two
weeks. Yes they can. But be sure you are using reasonably sterile technique so
they don't become contaminated. Then validate how long they last. I know of
one lab in the "old" days of manual staining that kept their
anager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Jesus Ellin [mailto:jel...@yumaregional.org]
Sent: Monday, November 28, 2016 7:47 AM
To: Willis, Donna G.; Morken, Timothy; Jennifer
Medical Center
-Original Message-
From: Jennifer Valentine-Williams
[mailto:jennifer.valentine-willi...@neogenomics.com]
Sent: Wednesday, November 23, 2016 2:02 PM
To: Morken, Timothy; Jesus Ellin
Cc: Histonet
Subject: RE: [Histonet] Personel
I would like to branch off from this top
extensive interpretation and judgment.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Jennifer Valentine-Williams
[mailto:jennifer.valentine-wi
nd they do not consider histology "processing"
to be a "test" and they consider any slide staining as "processing." So they do
not require competency testing for histology personnel (We do it anyway because
it is a good thing to do).
Tim
-Original Message
swer, most
of which are contradictory from one inspector to another.
Tim
-Original Message-
From: Curt [mailto:c.ta...@pathologyarts.com]
Sent: Tuesday, November 22, 2016 10:43 AM
To: Morken, Timothy; Jesus Ellin
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: Personel
I recently had
Jesus, that is very interesting information.
Does anyone know of any CAP accreditation documents that state explicitly that
IHC slide staining is high complexity? I have not seen any. If anyone has those
documents I'd like to see them. The only reference from CAP about that
classification I
Helen, we use Coleman 50-qt wheeled coolers (with wheels and handles). The
containers and buckets are parafilmed and put in plastic bags. The coolers have
formalin absorbing pads at the bottom for any leaks. The coolers are liquid
proof, easy to roll around. Occasionally a handle will break on
I agree, we use the IMEB bone saw as well. All human bones though...
-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, October 27, 2016 10:34 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Bone saw
We use an
Hi all, for those under Joint Commission, can you give your interpretation of
this standard having to do with QC of stains?
_
QSA 13.06.01
The equipment, methods and stains used in producing microscopic slides provides
tissue sections that facilitate a diagnosis
Elements
Do you mean the list required by COM.40200, or a validation procedure?
The list is just a list of those test you have modified from manufacturer's
instructions, or developed in-house from ASR's (any IHC test you do in which
you modify dilutions, detection, AR etc).
For validation, The FDA LDT
Nirmala, Histotechs are not required to have competency assessment under CLIA
regulations because, as you note, they do not interpret any tests or report any
results. However, if they do grossing then they do require high complexity
competency assessment.
Having said that, if there is some
Nirmala, Histotechs are not required to have competency assessment under CLIA
regulations because, as you note, they do not interpret any tests or report any
results. However, if they do grossing then they do require high complexity
competency assessment.
Having said that, if there is some
All knowing histonet. Does anyone have a lab contact at Kaiser Sunnyside,
Clackamas, OR? Their bureaucracy is impenetrable!!
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
Fawn, equipment we dispose of (ie, surplus for sale) we just have to clean it.
For a tissue processor we would run a cleaning cycle, clean up the outside of
crud and it is good to go. There is no infectious contamination issue. We
signoff on a form that it has been decontaminated. It is
Judi, what is the purpose of freezing it? For sectioning, biochemistry,
histochemistry, or shipping it? It makes a difference.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
That's a good question, but tough to answer because every lab has different
ways of mounting sections. I think you will have to define that for your own
workflow. I've seen labs that require 30 serial sections on a slide for certain
specimens, so what is that?!
You could start with the one
Our career ladder at UCSF
HLT 1 thru 4 (Hospital Laboratory Technician) for non-histology positions.
HLT-1 is entry level, could be out of high school. To move up requires more
education. HLT-2 AA to BA/BA, HLT-3, -4, BA/BS used as accessioners, basic lab
techs in grossing and cytology,
How do you handle counting days to preliminary autopsy dx (PAD) when doing
cases in which the brain autopsy results are the main component of disease? If
we accession at the time of autopsy and then let the brain fix for several days
before cutting in, then the PAD is many days past the 3-day
Seems like nurses would have enough to do already! I wonder if this is aimed
at physician labs where they may have a nurse but no lab tech...
California requirement for RN BS degree require only one quarter of
microbiology with lab, and one quarter of basic chemistry with lab. And 3
Charles, for most histology applications water from laboratory-grade DI and
distilled systems are fairly interchangeable because modern systems bring them
pretty close to the same purity. But some stains can be affected if they are
susceptible to chemical interactions with something left in the
Sakura has purpose-made scrapes. Otherwise you can use plastic scrapers from a
hardware store.
However, we moved away from scrapers and now use a heating block trimmer made
for the purpose. Several vendors offer these.
http://www.newcomersupply.com/product/paraffin-wax-trimmer
We do all
I guess one question is, how did they get the impression they would get the 5%?
That is not something that is normally written into the job description, but
should be documented in a job offer, or it is made clear in some policy that as
they gain competency, and the lab organization
Curt, we went through the same thing, but for the embedding we keep a plastic
bucket on the bench and lined with a biohazard bag, and put all the lids and
papers in that. We keep those for a week in case some tissue ends up missing.
Then it goes into the red cans.
At the microtome we do use
Tamara, Yes, it is possible, and some do immuno on plastic sections with this
method, but it's a lot of work and the issue arises of whether you have
validated your stains to work when this method is used. And do you have
controls treated the same way? In the clinical diagnostic environment, in
Kenneth, Sorry but no, it does not work to go the other way. We do this
occasionally but normally only a take a small portion of the tissue in the
block. Did they embed all of it in plastic?
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special
Here's another good document on how to handle picric acid powder
www.ehs.wisc.edu/chem/SafeHandlingOfPicricAcid.pdf
-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, May 06, 2016 8:28 AM
To: Julio Benavides
Cc: Histonet
Julio, you can just pour water into the container. We always oversaturated so
that a layer of water was on top of the powder.
Look at this explanation
http://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf
or read the text below if you cannot open this. This contains instructions
Cynthia, best practice is to keep in the block and cut as needed. Second is to
cut and dry at room temp and not melt, then store. Don't cut, melt and store.
The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect
the antigens better if they are fully isolated from air,
Travis, check out the CDC website
http://www.cdc.gov/hicpac/Disinfection_Sterilization/6_0disinfection.html
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original
Melissa, our PA's look at them when received in the grossing lab, and if they
aren't sure they bring them down the hall to the EM lab and we confirm. Our
clinicians seem to be pretty good at getting a good bx without examining them
in radiology so this works for us.
Tim Morken
Pathology Site
A warning on the Mount-Quick...
We have used this for years for our histochemistry slides but we stopped using
Quickmount recently when we got a batch that failed - it seemed a bit more
viscous than usual while coverslipping and then a couple months later all the
coverslips were found to be
We've been adapting the Leica Bond to do immunofluorescence staining and
finding it a hard road. Leica does not support IF staining within the standard
clinical software so we need to buy a FISH kit (just one vial of formamide) in
order to get a "kit" barcode to run the system. We dump the
Deanne,
If you just need it for a few critical reagents You can get type 1 water by the
pint or the gallon from Fisher (NERL Type 1 water). It's probably overkill for
most histology procedures, but convenient if you need it. Note that the expiry
clock of 30 days starts when you open the
Charles, Foreign course work is useless for CLIA until it is vetted by a
transcript service that can evaluate the validity of the school and how that
course work matches with the required typical US courses. ASCP requires such
vetting for foreign applications for ASCP certification (I've been
For those that knew him or took courses from him, Al was a consummate
professional. I was fortunate enough to work with him on some projects and
found his knowledge and insight invaluable. He was also a good friend. He will
be missed, especially as a fixture at NSH meetings.
Tim Morken
Maria,
If the counterstain is good when done before IHC stain and poor after it sounds
like proteins are being extracted during the IHC processing and staining. Have
you tried staining sections after each step of the IHC process to isolate the
point the stain becomes weak?
Tim Morken
Hi Karen,
We get ours from Sigma-aldrich:
Ammonium Sulfide, 25ml A1952-25Sigma-Aldrich $34.12 EA 25 ml
This is a 20% solution
We just got some so I know they have it.
We use a 10% solution of this, so it comes out to 2% final concentration: we
use 4ml of the ammonium
Hi all, we have some old beakers that we use for hot solutions that have cork
covering over the vertical portion of the beaker The cork insulates for easy
handling. I have not been able to find replacements for these. Does anyone have
a source for these beakers? Or a method to cover them with
The response to a problem like this is that your lab has not validated such a
procedure for diagnostic use so the results are completely unreliable with your
current protocols. Sure, you can "try it" but a negative result would mean
nothing at all. Or you might get massive background that also
Diana,
Obviously there are no negative controls for some special stains because they
just stain tissue elements (ie, trichrome) that are often in all tissues and we
are just looking at the morphology changes in a disease state. A negative would
have you apply the stain to tissue that could not
Banjo,
We use a form to inform pathologists/residents of tissue problems. Our SOP is
that anything that is noticed at embedding or sectioning that either
compromises quality or prevents proceeding is noted on the form and sent along
to the pathologist/resident. If we cannot proceed due to
What I believe happens is that the referring lab must do an audit/inspection of
the technical-only lab to be sure they are CLIA compliant. For instance, check
validation procedures for stains, equipment, quality control. It's true CLIA
itself does not inspect the Tech-only lab, but the
From: Histology [hi...@pathlab.us]
Sent: Monday, October 05, 2015 5:18 AM
To: Morken, Timothy
Subject: RE: GMS and Gram stains
1. Gram: Sigma-Aldrich Accustain
2. Gram: Hard to see gram negative
1. GMS: Grocott's Methenmmine Silver modified
2. GMS
Does anyone have a left-over computer for the Dako Autostainer Plus you want to
sell? Ours died and the service vendor is having a hard time finding a
replacement.
Any help is appreciated!
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special
Elaine you can download the powerpoint and all the handouts from the Advance
for Laboratories website under Webinars, QA/QC, " Basic Principles of
Validation in Histology"
Link is below:
I just bought one from IMEB :
http://www.imebinc.com/
Hello Histonet,
Does anyone know where I can find a cryostat vacuum? I've used them in the
past but can't seem to find a manufacturer.
Thanks!
Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre
General
Interesting. I can see the point if they want total opacity as to person ID. Is
only the number supposed to appear on the label? How about a second identifier?
It would not cause mistakes in labeling (because the labels come from the
system) as long as you are following one-piece workflow. But
Laura,
We don't hand-write anything on case slides before applying a printed label, if
that is what you mean. Hand-written labels are the most error-prone method of
labeling.
We use a TBS slide etcher to batch-print on-slide labels for our on-slide
control TMA's and single-tissue control
Anna, This is a great topic and you pretty much nailed it as far as getting
into it accidently. The fact is, most of us did. However, you can approach it
a couple ways.
The big question is, how do you want to advance. Do you want to stay in the lab
and be the overall lab expert, or do you
Does anyone have a source for NFPA laser printer labels. Especially in the 1 x
3 size?
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
Merissa,
Water 77-80 solvent
Formic acid 21-23 active ingredient
Fluorad 1surfactant - a wetting agent
to make the solution wet the bone more easily
Sodium citrate 1emulsifier , buffer
Paula, we use an Oakton Temp10K with a Type-K thermocouple probe (goes to
-250C) . We got it from Fisher scientific
https://www.fishersci.com/shop/products/oakton-digi-sense-dual-input-j-k-t-thermocouple-thermometer/p-203772
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron
, Christopher [mailto:sim...@upmc.edu]
Sent: Friday, July 10, 2015 12:44 PM
To: Morken, Timothy
Cc: Histonet
Subject: Re: [Histonet] Sakura SmartSection
Have it smart section undecalcified bone or teeth in plastic then I might give
it a look But only a look You can never take the art away from
Has anyone done any work with the Sakura SmartSection robot? We've had some
blocks cut on it and have had good initial results. This could be a
game-changer for histology staffing.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
it. Sakura demoed it at NSH last
year to select customers, and not at the general trade show.
Tim
-Original Message-
From: Willis, Donna G. [mailto:donna.wil...@baylorhealth.edu]
Sent: Friday, July 10, 2015 1:47 PM
To: 'Cooper, Brian'; Morken, Timothy; histonet@lists.utsouthwestern.edu
Subject
We had some a few years ago and thought it was contaminating bacteria, but
turned out to be dirty water from the di tap. We had our plumbers take it apart
and clean everything.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
All knowing Histonet
We started doing alkaline phosphatase on muscle frozen a little while ago and
have had an issue with apparent air bubbles forming over the tissue. Not
trapped air from coverslipping, but forming from apparent reaction in the
tissue. Does anyone have experience with
Dawn, I'd say 12-5 is a good TAT for any lab. Same day TAT is excellent. Maybe
they don't want to stay late to ready the slides?
We do 250 - 300/day, 5-6,000/month but we have extended working hours. We cut
recuts from 11pm thru to 3pm and start loading the Bond and Ventana at 4am.
Then they
Jill, I ordered custom made tables from OnePointe solutions. Pricing is very
reasonable
http://lab.onepointesolutions.com/custom-lab-tables/
[Histonet] LAB
Pam, we considered Cerebro a couple years ago but it was not really ready for
prime time yet. It did not have the capability to link with our Cerner Copath
Plus at that time.
However, we did go see it live in some stand-alone labs and it looked very nice
and well thought out
The two major
Patti, do remember any details of the following procedure We have a heart
pathologist interested
Thanks to you or anyone else that can help with this.
++
From 2005...
Kappa and lambda are certainly difficult to interpret. They both can be
present circulating in the serum,
A position is open for a lead technologist at UCSF Medical Center, San Francisco
The hours will be early morning to mid-morning.
Website: http://jobs.ucsfmedicalcenter.org/
Search Histotechnologist
Or
Job ID 8737
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron
Carole, I determine that by how critical it is to have it working, how long you
can go without, whether I have backup units and how long it takes to get
non-priority one-off repair service if something goes down. We do have
contracts on most of our equipment. If it is a common item that a
UC San Francisco has two Pathologist Assistant openings due to expansion of the
service.
Certified, experienced preferred, but recent graduates are encouraged to apply
One opening is for the Parnassus Campus at Moffitt Hospital (near Golden Gate
Park). The other is for the new Mission Bay
Same as Joyce, we accession as consult case (as a separate specimen class) and
do the whole barcoding routine on outside blocks and slides.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco
Mike, yes, the vast majority of histotechs have been, are, and will be OJT (me
included). The people who take on training these people have a responsibility
to do the best they can. Most techs end up learning whatever their lab does and
so have limited knowledge. I studied a full year for the
I think there is some actor from the CSI series that has done some of this work
promoting lab techs...
Tim Morken
-Original Message-
From: Pam Marcum [mailto:mucra...@comcast.net]
Sent: Thursday, May 14, 2015 9:18 AM
To: Lisa Roy
Cc: Histonet; Michael Dessoye
Subject: Re: [Histonet]
FYI, We are manually coverslipping frozen slides with tape, due to the fact it
dries very fast, and then scanning immediately for remote review. It is working
very well.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of
Blocks, yes. Parts of a case (specimens), no.
-Original Message-
From: Michael Mihalik [mailto:m...@pathview.com]
Sent: Friday, May 08, 2015 9:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Friday Trivia Question: Most specimen on a single case
Please excuse the trivia
Bernice, You have to buy the 10N solution. You can only dilute a given normal
solution, you cannot concentrate them.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
Jim, yes, in my experience you are going to use this for 70 to 95% alcohol
steps, not 100, unless you get a water absorber (BR has one, at least used
to).
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San
One pathologist said it looks like the tissue has been cooked. Which could
also be drying artifact after bx, before formalin.
The only issue is we can have two biopsies right next to one another in the
basket one looks good and one looks bad. My director also thinks it is the
processors.
The95 for HIER is in liquid, The 82in the oven is dry slides. While wet high
temps enhance HIER, It seems the high dry temp does harm epitopes (there is a
paper somewhere on this but I don't have access to it right now).
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular
Anyone doing cilia brush bx embedding for EM - do you have a proven method for
using agar/gel for the pellet to avoid manual processing? We tried Histogel but
it falls apart too easily so will move to trying agar. But if anyone has a
proven method it would be helpful to try it.
Thanks!
Tim
Free for anyone:
I have tons of left over white numbered beads that we used for embedding ID
before we went to barcoding. Numbered/lettered 0-9, A-E, various quantities but
generally many hundred of each. Any takers? I'll ship.
We got these from Cancer Diagnostics:
in a profession they
love.
Tim
-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org]
Sent: Wednesday, March 25, 2015 3:19 AM
To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] BS in Histotechnology
Tim for a Pathology
look at the situation in the US and say it is ok after seeing what
goes on elsewhere.
Tim
-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org]
Sent: Wednesday, March 25, 2015 3:19 AM
To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu
Subject: RE
Jennifer, we require a BA/BS degree for all Histotechnologist positions.
However, in our 4 step categories Level 1 does not require certification, just
the degree and the requirement that they get the certification within a year.
Advancement to level 2 to 4 requires an HT or HTL certification
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Tuesday, March 24, 2015 10:13 AM
To: Sue
Cc: Histonet; Morken, Timothy; Jennifer MacDonald
Subject: Re: [Histonet] BS in Histotechnology
It is the only truth I deal with here. We are, like TJH
the requirements for certification so does not need to be a
specialty degree.
Tim
-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org]
Sent: Tuesday, March 24, 2015 9:06 AM
To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] BS
Paula,
How many times per day?
Is the embedding close to the cutting area?
Of course any extra walking is a problem, especially in busy areas. Is this a
non-patient area (hopefully!)? Any restructuring should be to move things
closer together, not further away!
Having said that, If it comes
Try this article...
Acta Cytol. 2003 Nov-Dec;47(6):1043-4.
Alternative, cost-effective fungus-staining method for control slides in
cytology and histopathology.
da Silva VD1.
Author information
Abstract
OBJECTIVE:
To develop a cost-effective, reliable and safe method of providing fungal
control
I agree it sounds bad, But the reality is that all food products have loads of
dead or low count living bacteria. For example, milk is pasteurized to kill
them, but they are still in the milk.
Tim Morken
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Our clinicians use a brush of some sort. Unfortunately I don't know the brand.
But the bristles do not come off; they are pretty stout, are in a spiral
pattern and each bristle has a knob at the end. We just gently scrape the cells
off the brush into fixative in a petri dish and then pipet up
If you are using an automated stainer the plexiglas cover that they all have
will block UV light. For instance we run all our IF in a Dako stainer with the
slightly tinted Plexiglas and have not had any problems. If staining manually
in a tray, covering with something to block light is ok. On
Hi Bonnie, we use some from Brain Research laboratories. They are cardboard
flats without the dividers that are in the 20-slide types .
They have 2x3 slide mailers as well.
http://brainresearchlab.com/product-category/slide-storage/slide-folders/
Tim Morken
Supervisor, Histology, Electron
If you are a member of NSH you can get a pdf for free just by contacting the
staff at the NSH office. A great reason to join, if you have not already!
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
Tanya, in our Copath system we have field for Date Taken (ie,service Date) and
Date Received (in Pathology). So we account for items received during off hours.
Or puts holds fresh specimens in a fridge for pickup, or takes to a Cyto fridge
in some locations. Cyto specimens delivered after
Glad to know I'm not the only one who haunts the plasticware sections of stores
looking for the ideal container for the lab!
We use some Rubbermaid containers for the Peloris and VIP baskets, but they are
not fully 3 high. I suggest looking at Bed Bath and Beyond, Container Store,
all
Craig,
CLIA licensure gives your lab the authority to validate and use ASR's for
diagnostics. For something like her2 you will need to compare it to an existing
FDA approved antibody, run the statistically significant number of cases (range
of positive, and negatives) and show high
I agree. We got one of these a couple years ago and the techs love it. It is a
heated block on which you rub the cassette. The paraffin melts away. It is
especially good for preserving barcodes (but don't press the printed surface on
the heat block too long - you can soften the print and cause
We use Sensaphone for our older VIP5 tissue processors, Leica online system for
the Peloris processors and Awarepoint for refrigerators/freezers.
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
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