Re: [Histonet] Formaldehyde and Xylene Detector

[Goins, Tresa via Histonet]

We have used two types:

1.  Badges that are worn and then returned for analysis (Advanced Chemical 
Sensors, www.acsbadge.com has badges for both 
formaldehyde and xylene) and
2.  Badges that contain a color indicator that require no analysis, just a 
visual check (Chromair from Morphix Technologies)

Tresa

-Original Message-
From: Arun Jyothi S.P via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, July 25, 2016 11:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formaldehyde and Xylene Detector

Hi Readers

Our lab would like to purchase formaldehyde and Xylene detectors as a part of 
accreditation.

Please share the specification and manufacturer of the detectors that you are 
using or you know of.

I look forward to hearing from you

Thank you
With Regards
Arun
Al Seef Hospital
Kuwait
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Re: [Histonet] Coverslipping mystery

[Goins, Tresa]

I am not familiar with DPX, but is there a compatibility issue between it and 
the slide clearant you are using?

Tresa

-Original Message-
From: Adam Boanas [mailto:a.boa...@epistem.co.uk] 
Sent: Thursday, July 09, 2015 7:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Coverslipping mystery

Hello,

We are having a problem that is developing into a big issue in our lab and I 
was wondering if anybody could shed any light on it. Our CV5000 coverslipper 
has recently started introducing microscopic air bubbles onto the slides during 
coverslipping. We have been told by our engineer that it is a consequence of 
the age and use of the motor and that sourcing another for an instrument that 
old (15yrs) will be v difficult. As such, we have been forced to manually 
coverslip using DPX and a pipette - manually applying the coverslips to the 
slide, thus mirroring the action of the coverslipper. This is fine at first and 
for the next few days the slides look great and very clean. However, after 
about day 4 -5 days post coverslipping, the slides develop an odd appearance 
down the microscope which looks like very fine `parched earth / crazy paving` 
all over the slide - including the section. The excess mountant around the edge 
of the coverslip also has a very faint, cloudy appearance when this o
 ccurs. This of course renders the slide un-useable. Does anyone have a clue 
what this might be down to / how we can stop it?
We are struggling for ideas with this one!  - this occurs with fresh DPX also.

Many thanks
Adam

Adam Boanas
Senior Research Associate
Epistem Ltd
48 Grafton Street
Manchester, M13 9XX

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Re: [Histonet] IHC on fish

[Goins, Tresa]

Cate -

Your gut is probably right but results are unpredictable.
I have forwarded your query to the Bozeman Fish Technology center to see if 
they can offer any assistance.

Tresa

-Original Message-
From: Hardy, Cate [mailto:cha...@csu.edu.au] 
Sent: Sunday, June 14, 2015 4:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC on fish

Hi all;

Has anyone performed IHC on fish. I have been asked to perform this using 
several of our routine antibodies that I use on mammals. My gut tells me it 
won't work but I am a lowly histotech and don't know anything. Seeking 
knowledge from learned tech's with experience in such matters

Thanks

Cate Hardy
Senior Technical Officer
Veterinary Diagnostic Laboratory
Charles Strut University
NSW Australia
Charles Sturt University

| ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | ONTARIO 
| ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA |

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Re: [Histonet] Specimen Accessioning .....

[Goins, Tresa]

We ID the specimens with numbers OR letters to differentiate between type of 
sample received.  
The ID on the specimen workup sheet is always the same as the ID on the block 
and slide.

A single large mass cut into multiple pieces is numbered 1, 2, etc.
Different masses from a single accession are differentiated by letters; if 
large enough for multiple sections per mass, they are labeled A1, A2 etc.

If we receive specimens with unique ID, e.g. cervical, thoracic and lumbar, we 
label C1, C2 . .  T1, T2 . . etc. to eliminate the need for the pathologist to 
refer to the worksheet to determine which slides are which.

Tresa


 
-Original Message-
From: Cartun, Richard [mailto:richard.car...@hhchealth.org] 
Sent: Wednesday, June 03, 2015 1:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Specimen Accessioning .

We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and 
the corresponding paraffin blocks as A, B, C, etc.  We are in the process of 
changing this to letters for the different specimen parts and numbers for the 
blocks.  For those of you who do it this way, have you encountered any 
problems?  For example, if the specimen arrives and the different parts are 
numbered, do you simply convert them to letters?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] plants in the lab

[Goins, Tresa]

Patients do not have to go to the fungal spores, the spores will go to the 
patient.
Depending on spore size, the spores may stay airborne for months - the spores 
sediment to a surface in still air. 
A condition not likely to occur in a hospital environment - they scurry around 
until finding a lung or mucous membrane to adhere to.
It doesn't take long for a single miss-handled Aspergillus culture plate to 
contaminate an entire multi-story research lab.



-Original Message-
From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au] 
Sent: Tuesday, June 02, 2015 3:20 PM
To: Michelle Lamphere; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] plants in the lab

Hi Michelle,
Why would patients be in a histo lab anyway?


From: Michelle Lamphere [michelle.lamph...@childrens.com]
Sent: Sunday, 31 May 2015 10:36 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] plants in the lab

Our hospital Safety and Infection Control departments have policies in place 
prohibiting any potted plants from being in the hospital, anywhere.  We can 
have them if they are only in water, but the soil presents an infection control 
issue for patients because of potential mildew, mold, spores, etc.


Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
O: 214.456.2318 | Fax: 214.456.0779
E: michelle.lamph...@childrens.com
1935 Medical District Drive | B1.06  | Dallas, Texas  75235



Message: 2
Date: Fri, 29 May 2015 14:23:00 -0400
From: Blazek, Linda lbla...@digestivespecialists.com
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: [Histonet] plants in the lab
Message-ID:

5a2bd13465e061429d6455c8d6b40e391742126...@ibmb7exchange.digestivespecialists.com

Content-Type: text/plain; charset=us-ascii

Happy Friday all!

Does anyone have documentation of the benefit of having plants in the lab?  I 
know this was discusses quite a while ago but I can't find references for it.  
Any help would be appreciated.

Thanks,
Linda


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Re: [Histonet] Toluidine blue

[Goins, Tresa]

We use a canine mast cell tumor as positive control - veterinary lab naturally.
Probably looking for mast cells in the core.

Tresa


-Original Message-
From: Bernice Frederick [mailto:b-freder...@northwestern.edu] 
Sent: Tuesday, June 02, 2015 12:02 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Toluidine blue

Hello all,
I was taught to do Toluidine Blue O without a control. Is there actual one and 
what would it be? I'm staining a bone core. Don's ask why, it's research and 
what a researcher wants... Plus they have a protocol they are following for 
this cartilaganous defect.
Thanks,
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edumailto:b-freder...@northwestern.edu

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Re: [Histonet] Question

[Goins, Tresa]

All that matters here is the final concentration of the reagent - it doesn't 
matter what stock you start with if you calculate the dilution.
Adding 1.25 ml to 1000 ml is diluted by a factor of .00125 so 10N x .00125 = 
.0125 N final concentration.
If using a 1 N stock solution, just add 10X the volume or 12.5 ml 1 N stock to 
987.5 ml water.

Hope this helps,

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice 
Frederick
Sent: Thursday, April 30, 2015 1:35 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question

All,
I have a procedure here that call for and I quote 1.25 ml NaOH 10N in 1L of 
water. I know how to get 1 N, but how do I get 10. Having rarely hd the 
opportunity to make many Normal solutions ,my brain is not computing. Is it an 
error?
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edumailto:b-freder...@northwestern.edu

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Re: [Histonet] Question

[Goins, Tresa]

The final concentration of 1.25 ml 10N NaOH into 1000 ml water is the same as:
12.5 ml 1N NaOH into 987.5 ml water.

-Original Message-
From: Geoff [mailto:mcaul...@rwjms.rutgers.edu] 
Sent: Friday, May 01, 2015 9:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Question

Huh?
Take a solution more dilute than you want it and dilute it more?

Geoff

On 5/1/2015 10:41 AM, Goins, Tresa wrote:
 All that matters here is the final concentration of the reagent - it doesn't 
 matter what stock you start with if you calculate the dilution.
 Adding 1.25 ml to 1000 ml is diluted by a factor of .00125 so 10N x .00125 = 
 .0125 N final concentration.
 If using a 1 N stock solution, just add 10X the volume or 12.5 ml 1 N stock 
 to 987.5 ml water.

 Hope this helps,

 Tresa

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice 
 Frederick
 Sent: Thursday, April 30, 2015 1:35 PM
 To: Histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Question

 All,
 I have a procedure here that call for and I quote 1.25 ml NaOH 10N in 1L of 
 water. I know how to get 1 N, but how do I get 10. Having rarely hd the 
 opportunity to make many Normal solutions ,my brain is not computing. Is it 
 an error?
 Bernice

 Bernice Frederick HTL (ASCP)
 Senior Research Tech
 Pathology Core Facility
 Robert. H. Lurie Cancer Center
 Northwestern University
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723
 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu

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-- 
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732) 235-4583; fax: -4029
mcaul...@rwjms.rutgers.edu
**



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Re: [Histonet] GMS Question

[Goins, Tresa]

To get a positive PAS or GMS fungal stain, one must oxidize the carbohydrate in 
the fungal cell wall.  
Chromic acid is a stronger oxidizer than periodic acid, so would work better 
with mature fungal cell walls that are highly polymerized.
Treat an immature cell wall for too long, and you may get a false negative 
because the carbohydrate structure no longer resembles a fungal cell wall.

Tresa

-Original Message-
From: Paula Lucas [mailto:plu...@biopath.org] 
Sent: Friday, May 01, 2015 8:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GMS Question

Hello,

 

I think I already know the answer but I'm not sure why so if someone can help 
me understand the theory behind it, I would greatly appreciate it.

 

Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic 
Acid as the 1st step.  

We use a control tissue from a case we had that was positive for fungus and 
it's a fungus ball from the Rt Maxillary. 

We ran a test for fungus on a different and current case of the same tissue 
(different patient): Rt Maxillary sinus.

 

The control tissue did work, but the patient's tissue did not, so the doctor 
ordered a PAS for fungus and this clearly showed the fungal elements nicely.


 

My question is why would the control and patient tissue have different results 
when they are both fungus balls from the same specimen source?

 

Thanks in advance,

Paula

Lab Manager

Bio-Path Medical Group

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Re: [Histonet] GMS Question

[Goins, Tresa]

Hi Gayle -

We have never had a noticeable problem detecting fungi using 5% aqueous 
chromic acid at room temperature for 1 hr.  The presence of mature and immature 
wall structures may be the reason. We have never tried to determine the end 
point for over oxidation, but my best guess is that it is much longer than 60 
minutes.  

More often, I think a false negative would result from inadequate oxidation of 
the fungal cell wall. 
In addition to cell wall maturity, I believe the composition adds to 
differences in rates of oxidation, the major contributors being the prevalence 
of precursors susceptible to aldehyde formation and melanin.  One needs enough 
product to produce a visible color reaction.

A complex question and any more detail and I will have to hit the books.

Tresa




-Original Message-
From: Gayle Callis [mailto:gayle.cal...@bresnan.net] 
Sent: Friday, May 01, 2015 1:17 PM
To: Goins, Tresa; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] GMS Question

Hi Tresa, 

What staining parameters do you suggest for seeing  mature and/or immature
fungal cell walls?   I don't think you will know what level of maturity is
present before doing a fungus stain.  But wouldn't both levels of maturity
both be present if the fungus is actively growing in a tissue?  

What do you recommend, using both a PAS, or chromic acid/Schiffs along with
chromic acid GMS method?   

We used 4% chromic acid at RT for 1 hour but would you recommend duplicating 
slides so you do pull one slide out of chromic acid at 30 minutes and one for 
60 minutes to stain either mature or immature fungal and/or both cell
wall structure.   

Gayle Callis
HTL/HT/MT (ASCP) 



-Original Message-
From: Goins, Tresa [mailto:tgo...@mt.gov]
Sent: Friday, May 01, 2015 11:29 AM
To: Paula Lucas; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] GMS Question

To get a positive PAS or GMS fungal stain, one must oxidize the carbohydrate in 
the fungal cell wall.  
Chromic acid is a stronger oxidizer than periodic acid, so would work better 
with mature fungal cell walls that are highly polymerized.
Treat an immature cell wall for too long, and you may get a false negative 
because the carbohydrate structure no longer resembles a fungal cell wall.

Tresa

-Original Message-
From: Paula Lucas [mailto:plu...@biopath.org]
Sent: Friday, May 01, 2015 8:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GMS Question

Hello,

 

I think I already know the answer but I'm not sure why so if someone can help 
me understand the theory behind it, I would greatly appreciate it.

 

Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic 
Acid as the 1st step.  

We use a control tissue from a case we had that was positive for fungus and 
it's a fungus ball from the Rt Maxillary. 

We ran a test for fungus on a different and current case of the same tissue 
(different patient): Rt Maxillary sinus.

 

The control tissue did work, but the patient's tissue did not, so the doctor 
ordered a PAS for fungus and this clearly showed the fungal elements nicely.


 

My question is why would the control and patient tissue have different results 
when they are both fungus balls from the same specimen source?

 

Thanks in advance,

Paula

Lab Manager

Bio-Path Medical Group

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[Histonet] RE: need light for flotation bath

[Goins, Tresa]

Try Light Bulb Surplus at www.lightbulbsurplus.com.  
I got replacement bulbs for less than $5.00 rather than $75.00 from a 
scientific supply vendor.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Preiszner, 
Johanna
Sent: Monday, March 02, 2015 8:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] need light for flotation bath

Hi,

we have a Fisher Tissue Prep 135 flotation bath and the light died. The 
instrument is no longer manufactured and I cant find a source for the 
fluorescent light tube. There are multiple baths for sale on ebay but nobody 
sells the light alone.

Could someone help me out? Would something from Home Depo do?


Thanks,
Hanna




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RE: [Histonet] CD68 and CD204 for dogs/pigs

[Goins, Tresa]

I just received Cd204 from Biorbyt but have not validated a protocol.
They are located in the UK but have a distributor in San Francisco so shipping 
is not outrageous.  

References to its application: 

The class A macrophage scavenger receptor CD204 is a useful immunohistochemical 
marker of canine histiocytic sarcoma.
J Comp Pathol 2013 Feb 16;148(2-3):188-96. Epub 2012 Aug 16.
Y Kato, M Murakami, Y Hoshino, T Mori, K Maruo, A Hirata, T L D R Nakagawa, T 
Yanai, H Sakaiand 


Immunocytochemical detection of the class A macrophage scavenger receptor CD204 
using air-dried cytologic smears of canine histiocytic sarcoma
Yuki Kato1, Risa Funato1, Akihiro Hirata, Mami Murakami, Takashi Mori, Koji 
Maruo, Tokuma Yanai and Hiroki Sakai 
Article first published online: 28 AUG 2014


If you or anyone has had success with this antibody, please let me know.

Thanks,

Tresa



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: Tuesday, February 24, 2015 3:59 PM
To: histonet
Subject: [Histonet] CD68 and CD204 for dogs/pigs

Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on 
FFPE tissue)?  I don't seem to find much information on species 
cross-reactivity online.

Thanks much in advance.

--
Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu
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RE: [Histonet] IHC

[Goins, Tresa]

[Cost of the reagent / (Volume of stock reagent / volume used per slide)].  

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Thursday, February 12, 2015 7:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC 


I am new to IHC, can anyone explain an easy way to calculate the equation of 
IHC cost per slide?  Thank you for your help...

Sincerely,

Craig

Sent from my iPhone
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RE: [Histonet] Re: Embedding

[Goins, Tresa]

Wow! Now I really feel lucky.  My cheap para trimmer came with a wax catcher 
with disposable liners that is magnetically attached to the trimmer.
http://www.tedpella.com/histo_html/histo1.htm.aspx#_28154   

Cant't beat $40 and a practical mind-set though.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Thursday, January 22, 2015 11:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Embedding

We use empty pipette tip boxes.  They are the perfect size and fit right under 
the edge and we just toss them when them get full.

Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Thursday, January 22, 2015 10:14 AM
To: gayle.cal...@bresnan.net; 'Tony Auge'; histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa'
Subject: RE: [Histonet] Re: Embedding

We use a urine specimen container under the right lower corner of the para 
trimmer and toss it each day.

Debbie M. Boyd HT (ASCP) | Chief Histologist  | Southside Regional Medical 
Center | 200 Medical Park Blvd.  |  Petersburg, Va.  23805 | PH 804-765-5025 | 
FAX 804-765-6058


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Gayle Callis 
[gayle.cal...@bresnan.net]
Sent: Thursday, January 22, 2015 1:03 PM
To: 'Tony Auge'; histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa'
Subject: RE: [Histonet] Re: Embedding

A fabulous idea!   I suspect one could use a cheap travel iron although  one 
needs to devise a way to collect melted paraffin.   Even our fancy para trimmer 
didn't have catch pan for paraffin drippings.I suggest using a new or 
receycled aluminum baking pans available in supermarkets, discount stores or a 
recycled frozen food pan without separations.  These pans come in various sizes 
and depths.   The joy is toss pans when full of paraffin.

Gayle Callis
HTL/HT/MT(ASCP)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge
Sent: Thursday, January 22, 2015 9:58 AM
To: histot...@imagesbyhopper.com
Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa
Subject: Re: [Histonet] Re: Embedding

If you want a cheaper alternative you can use a ski wax iron. They cost about 
$40. I mounted one upside down in a bucket and it works just as well as the 
$500 para trimmers.



-Tony Auge HTL (ASCP) QIHC
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RE: [Histonet] Re: Embedding

[Goins, Tresa]

I agree with Gayle.  We finally purchased a trimmer from Ted Pella - lowest 
price by far - and are saving our finger joints.  The amount of wax remaining 
on the cassette also appears to depend on the brand of mold used.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Wednesday, January 21, 2015 9:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Embedding

After years of never winning the battle of paraffin on cassette edges after 
embedding,  we purchased a paraffin block trimmer.  It saves time and the 
stress on finger joints compared to scraping cassettes daily.  No matter how 
careful we were during embedding to keep excess paraffin off cassette edges,
we were never successful.   Several vendors have these and you may be able
to find a refurbished one.   

 

Gayle M. Callis

HTL/HT/MT(ASCP)

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RE: [Histonet] Block Counts

[Goins, Tresa]

Depends on the type of tissue.
Depends on the length of time your day is for a repetitive task.
Assigning an arbitrary number is counterproductive.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen
Sent: Monday, January 12, 2015 1:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Block Counts 

I'm looking for raw data on time studies that directly deal with the number of 
blocks a PA can produce in a day and how many a histo tech can cut a day. 


Thanks

Sent from my iPhone
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[Histonet] RE: Research cases

[Goins, Tresa]

We charge by the hour.  The condition of the samples when received varies 
greatly (container, sample ID, master list, etc.) and by the hour rewards those 
who are organized and charges a fair rate for those who are not so organized. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Thursday, January 08, 2015 7:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Research cases

Good morning everyone!

I wanted to ask what others are doing about charges when blocks  slides are 
being requested for research purposes. We have been asked to provide a cost for 
this service, because the research companies are willing to pay the lab for our 
time processing, embedding, and cutting slides specifically for this purpose. 
Any guidance in this would be greatly appreciated!


Toni Rathborne
Pathology Supervisor

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[Histonet] RE: Water baths

[Goins, Tresa]

We use the range 2C to 8C - the range published for products requiring 
refrigeration in the Sigma catalog.

tg

  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Timm, Amber L
Sent: Wednesday, December 03, 2014 9:09 AM
To: Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; 
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Water baths

What is everyone using as their histo refrigerator temperature range?

Amber Timm, MLS (ASCP)CM

Supervisor of Laboratory Technical Services

The Diagnostic  Treatment Center

3401 Cranberry Blvd.

Weston, WI 54476

Phone: 715-393-2485

Email: ti...@dxandtx.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Tuesday, December 02, 2014 10:50 AM
To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Water baths

We still do, due to the fact that it is on our general temperature chart that 
is used in the department.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, December 02, 2014 11:20 AM
To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Water baths

It is no longer a CAP requirement to do this, so we haven't for many years.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Tuesday, December 02, 2014 10:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water baths

Does anyone take daily temperature checks of their water bath (used for cutting 
only) for CAP?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 
19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] RE: slides for IHC's

[Goins, Tresa]

Mercedes Medical Starfrost adhesive for IHC and HE

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen 
- SMMC-SF
Sent: Wednesday, October 29, 2014 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] slides for IHC's

I would like to know what kind of slides everyone else is using for their 
IHC's.  I am having some problems with tissue falling off the slides during 
Target Retrieval.  This happens mostly on fattier tissue.

Thanks,

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
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[Histonet] RE: biopsy bags for processing - alternatives

[Goins, Tresa]

For pesky animal hair (we are a veterinary lab) with very little tissue 
attached, we wrap in lens paper - one layer of paper on one side of tissue and 
all other layers of the paper envelope on other side of the tissue - and embed 
the entire package.  

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, August 13, 2014 9:24 AM
To: Histonet
Subject: [Histonet] biopsy bags for processing - alternatives

All knowing Histonet,

Our grossing staff uses nylon biopsy bags to enclose some biopsy specimens. 
The embedding staff find them troublesome because when they pull the bags open 
they tend to pop open and throw the tissue off in all directions. They have 
to be very careful opening these. Is there another bag made of some other 
material that is less prone to this problem?

For various reasons some of these samples can't be put on sponges. They do wrap 
some in flat biopsy paper, but not others. It seems to be a grossing personal 
preference more than anything else.

Thanks for any and all info!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org


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[Histonet] RE: Rabies tissue Fixed in Formalin.

[Goins, Tresa]

Sheryl -

NBF will inactivate the rabies virus but precautions should be taken.
If a rabies case is suspected but infection can not be confirmed or ruled out 
due to the tissue being unsuitable for rabies testing by fresh tissue 
fluorescent antibody assay, the tissue is cut in for FFPE sections ONLY by 
personnel that are vaccinated against rabies.

Tresa

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephenson, 
Sheryl
Sent: Thursday, June 05, 2014 12:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rabies tissue Fixed in Formalin.

Hi,
Does anyone have any knowledge and literature on whether or not 10%NBF 
inactivates rabies in animal tissue during fixation?
Thanks,
Sheryl.





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RE: [Histonet] IHC wash with dH20 / follow-up

[Goins, Tresa]

I may be wrong but I think that the:
Electrostatic benefit is imparted by the salts in the buffer
The surfactant benefit is imparted by the detergent



-Original Message-
From: Kim Donadio [mailto:one_angel_sec...@yahoo.com] 
Sent: Tuesday, March 18, 2014 12:15 PM
To: Goins, Tresa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC wash with dH20 / follow-up

Remember you are doing them by hand do you can manually place reagent where you 
want it. Buffer would be necessary on a machine if for nothing else other than 
as a surfactant to insure all corresponding reagents spread evenly on the 
slide. 

I just wanted to add this note in case some did not catch that this conclusion 
was for doing by hand. Although my opinion is that even by hand it is best 
practice to use a buffer due to previous comments about electrostatic benefits 
of using a surfactant. 

My two cents :) 

Kim 

On Mar 17, 2014, at 3:42 PM, Goins, Tresa tgo...@mt.gov wrote:

 Thanks for the responses to my question re IHC wash without buffer.  It made 
 me examine the question more thoroughly with empirical comparisons.
 
 The Dako IHC manual is a good reference.  But, the take home message I got 
 after reviewing it didn't alter my view that buffer wash may not be required. 
  Following the initial electrostatic interaction that occurs between antibody 
 and antigen in the milieu of the antibody diluent, the Ab-Ag bond is 
 maintained via Van der Waals and hydrophobic forces.  No matter what the 
 slide is rinsed with, I don't think this microenvironment is going to be 
 altered.
 
 In our hands, IHC results with twenty antibodies has not been adversly 
 effected by using dH2O in place of wash buffer (TBST in our case) - staining 
 intensity and contrast with the water wash is equivalent to buffer washed 
 slides.
 
 I located a reference that supports the use of a water wash (Hallelujah - I'm 
 not crazy) in:
 
  TECHNICAL IMMUNOHISTOCHEMISTRY:Achieving Reliability and Reproducibility 
 of Immunostains.
 
  Rodney T. Miller, M.D.
  Director of Immunohistochemistry
  ProPath Laboratory, Inc.
  8267 Elmbrook, Suite 100, Dallas, Texas 75247-4009
  Phone (214) 237-1631 FAX (214) 237-1770
  www.propathlab.com E mail: 
 rmil...@propathlab.commailto:rmil...@propathlab.com
 
An excerpt from the reference states:
 
Although previously I never thought of using anything else for 
 rinsing steps, a conversation with another immunohistochemist (Dave Tacha, 
 BioCare, Walnut Creek, CA) led me to try distilled water with Tween 20 
 (DW/Tween20) rather than PBS for the rinsing steps.  When we evaluated the 
 performance of DW/Tween 20 for our rinsing solution between steps, it 
 performed just as well as using PBS with Tween 20, so we now routinely use DW 
 with 0.2% Tween 20 (2 ml Tween 20 in 10 liters of DW) for all of our rinses 
 that previously used buffer.  This has saved us substantial amounts of 
 reagent cost as well as reagent preparation time, since preparing DW/Tween 20 
 is far easier and cheaper than preparing our previous PBS buffer rinsing 
 solutions.
 
 OK, detergent is still present, but with polymner-based IHC, background has 
 not been a problem . . . . .yet.
 At the very lease, the cost of Tween 20 and distilled water is a whole lot 
 cheaper than Tween 20 and buffer.
 
 Happy validating!
 
 Tresa
 
 
 
 
 Tresa Goins
 Histopathology Section
 Montana Veterinary Diagnostic Lab
 Bozeman, MT 59715
 406-994-6353
 
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[Histonet] IHC wash with dH20 / follow-up

[Goins, Tresa]

Thanks for the responses to my question re IHC wash without buffer.  It made me 
examine the question more thoroughly with empirical comparisons.

The Dako IHC manual is a good reference.  But, the take home message I got 
after reviewing it didn't alter my view that buffer wash may not be required.  
Following the initial electrostatic interaction that occurs between antibody 
and antigen in the milieu of the antibody diluent, the Ab-Ag bond is maintained 
via Van der Waals and hydrophobic forces.  No matter what the slide is rinsed 
with, I don't think this microenvironment is going to be altered.

In our hands, IHC results with twenty antibodies has not been adversly effected 
by using dH2O in place of wash buffer (TBST in our case) - staining intensity 
and contrast with the water wash is equivalent to buffer washed slides.

I located a reference that supports the use of a water wash (Hallelujah - I'm 
not crazy) in:

  TECHNICAL IMMUNOHISTOCHEMISTRY:Achieving Reliability and Reproducibility 
of Immunostains.

  Rodney T. Miller, M.D.
  Director of Immunohistochemistry
  ProPath Laboratory, Inc.
  8267 Elmbrook, Suite 100, Dallas, Texas 75247-4009
  Phone (214) 237-1631 FAX (214) 237-1770
  www.propathlab.com E mail: 
rmil...@propathlab.commailto:rmil...@propathlab.com

An excerpt from the reference states:

Although previously I never thought of using anything else for 
rinsing steps, a conversation with another immunohistochemist (Dave Tacha, 
BioCare, Walnut Creek, CA) led me to try distilled water with Tween 20 
(DW/Tween20) rather than PBS for the rinsing steps.  When we evaluated the 
performance of DW/Tween 20 for our rinsing solution between steps, it performed 
just as well as using PBS with Tween 20, so we now routinely use DW with 0.2% 
Tween 20 (2 ml Tween 20 in 10 liters of DW) for all of our rinses that 
previously used buffer.  This has saved us substantial amounts of reagent cost 
as well as reagent preparation time, since preparing DW/Tween 20 is far easier 
and cheaper than preparing our previous PBS buffer rinsing solutions.

OK, detergent is still present, but with polymner-based IHC, background has not 
been a problem . . . . .yet.
At the very lease, the cost of Tween 20 and distilled water is a whole lot 
cheaper than Tween 20 and buffer.

Happy validating!

Tresa




Tresa Goins
Histopathology Section
Montana Veterinary Diagnostic Lab
Bozeman, MT 59715
406-994-6353

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[Histonet] IHC without wash buffer

[Goins, Tresa]

In a continuing effort to limit the volume of reagent used in each step of a 
manual IHC, I have tried TBS and TBST on slides with barriers (expensive) and 
without barriers.
The results were not stellar with the barrier slides - the reagent still 
escapes.  We dewax with hot detergent (may be a contributing factor) and the 
Tween 20 in the TBST definitely alters the hydrophobicity of the barrier, an 
effect that is not reversed with a water wash.

Consequently, I have resorted to omitting the buffer wash steps and using 
distilled water only.  The slide surface remains water repellent and the 
added IHC reagents form a pool over the tissue sections.
The detection method is a polymer-HRP and there is no increase in background 
staining in the tissue or on the slide surface.

I am assuming that the IHC reagents are prepared in the optimum suspension 
liquid and a buffer is not required.  So, I am interested in hearing from 
anyone why this is a bad idea.

Thanks,

Tresa






[X]
[X]




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[Histonet] Mailing Freebie

[Goins, Tresa]

OK - I may be missing something, but what is that blue adhesive pocket that 
was sent out with histology professionals' day literature?
Just curious . . . . .

Happy Friday.

Tresa





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RE: [Histonet] Counterstain on Ventana Benchmark Ultra

[Goins, Tresa]

We manually stain, but may switch from hematoxylin to Acid Blue 19 to increase 
contrast with the red chromogen preferred by the pathologists.

Zehntner et al 2008.  Synergistic tissue counterstaining and image segmentation 
techniques for accurate, quantitative immunohistochemistry.  Journal of 
Histochemistry and Cytochemistry 56(10):873-880.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Susan Foreman
Sent: Thursday, December 19, 2013 10:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Counterstain on Ventana Benchmark Ultra

Merry Christmas to all those out there in Histoland!  

 

How are other labs handling the counterstain for IHC/ICC stained slides on the 
Ventana Benchmark Ultra?  Has anyone figured out how to counterstain online 
without purchasing Hematoxylin and Bluing directly from Ventana?  Any
sort of prep kit ?   Our slides are beautiful, just hoping to reduce our
cost/test.

 

Many Thanks,

 

Susan Foreman, HT (ASCP)
(865)661-4734

(865)584-1933

 

 

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[Histonet] RE: Nails for fungal stain

[Goins, Tresa]

Formalin does not affect carbohydrate structure so when the nail is fixed 
shouldn't matter.  
The KOH digests proteins so no deleterious effect on the fungal carbohydrate 
pre- or post-fixation.
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Friday, December 13, 2013 10:01 AM
To: Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Nails for fungal stain

Our lab receives toenails, and we routinely do an HE stain and a PAS stain for 
fungus.  We receive the nails fresh, and when they are grossed they are placed 
directly into a purchased nail softening solution that is mainly potassium 
hydroxide before being placed on the tissue processor.  My question:  Would the 
potassium hydroxide have any bearing on the results of the fungal PAS since the 
nails are not fixed in formalin before going into the potassium hydroxide?

Laurie Colbert, HT (ASCP)

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[Histonet] RE: looking for a product

[Goins, Tresa]

If you don't want to use a knife blade to trim the excess wax, embed at one end 
of the mold and use the hot plate to remove excess wax on the other end. 
Only problem may be getting a ribbon if one edge of the trim surface is not 
straight.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bond, Lee
Sent: Thursday, December 12, 2013 7:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] looking for a product

Hi All-



Can anyone tell me where I can purchase histology base molds in a long, thin 
rectangular shape? We would like to be able to get more cuts of a prostate 
biopsy onto a single slide. We currently are using this shape of a base mold 
and having to manually trim excess wax (dangerous and time consuming):

_

/   /

/  **   /

/  **   /

/  **   /

/  **   /

/  **   /

/  **   /

/  **   /

/___/



We would like to find base molds shaped like this, so that we eliminate the 
need to manually trim each block:

_

/   /

/   /

/   /

/  **   /

/  **   /

/  **   /

/   /

/   /

/___/



My staff would be incredibly appreciative for any help in finding this product!



Thanks all-



Lee Bond, MT(ASCP)

SFHC Lab

Topeka, KS

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[Histonet] RE: IHC Buffer (wash)

[Goins, Tresa]

Try ScyTek - The ready-made price is nearly as cheap as I can make it myself.  
http://www.scytek.com/default.asp 

Tresa




Tresa Goins
Histopathology Section Supervisor
Montana Veterinary Diagnostic Lab




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty
Sent: Wednesday, December 04, 2013 3:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Buffer (wash)

Hello everyone,

Got your Christmas shopping done? (Didn't think so...) 

Anyway, I'm looking for an IHC PBS/Tween buffer wash concentrate that is 
comparable to the very expensive Lab Vision PBS/Tween buffer wash. I see some 
at VWR and FISHER (no shipping charges for our lab) that are 0.5% and 1% Tween 
20 washing buffer in PBS.

Which percentage is best for IHC on a Lab Vision stainer using 
a polymer detection kit? As usual, your input is appreciated.

Sandy

 

P.S. I know I can make up my own from scratch, just not interested in doing 
that at the moment...

 

Sandra Cheasty

Histology  Necropsy Supervisor

UW-Madison, School of Veterinary Medicine

608 263-1680 (Office)

608 263-1055 (Lab)

 

 

 



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[Histonet] RE: Embedding Paraffin

[Goins, Tresa]

Paraplast X-tra by McCormick.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark
Sent: Tuesday, November 12, 2013 8:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Embedding Paraffin

What  paraffin does everyone like for embedding?  We are currently using 
Surgipaths EM-400 but its dirty!Who has a clean, easy to section paraffin 
that they like?

Thanks!


Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-3982
mro...@sfmc.net
http://www.sfmc.net



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RE: [Histonet] Re: India Ink for inking surgical margin borders

[Goins, Tresa]

Tattoos Yucky?  Guess I'm yucky.  I got yucky way before tattoos became popular 
when the response was more akin to fringe element.

Happy member of the Fringe.

:)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Thursday, October 10, 2013 11:43 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: India Ink for inking surgical margin borders

Ed Crespo, CT(ASCP) in Cypress CA asks: I normally purchase India Ink from 
one of our vendors, but know it's also sold at artist supply shops.
Does anyone know if I can use the artist india ink for Pathology?  Really, the 
only issue would be if the ink stays on the tissue during processing right? 
Please advise.

I've used india ink from artist supply stores for marking surgical margins, for 
many years, and it's entirely satisfactory.

A cheap source of colored particulate inks is tattoo inks - available in a huge 
range of colors - I have one pathologist client who's used them for years. The 
only downside is that you have to read some seriously yucky catalogs.

Bob Richmond
Samurai Pathologist
Maryville TN
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RE: [Histonet] The Good Old Days...

[Goins, Tresa]

I've stropped blades until they looked like scythes - and I got pretty good at 
adjusting those three screws to line up my block on the microtome.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Friday, September 20, 2013 12:15 PM
To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] The Good Old Days...

How many can strop a knife?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias
Sent: Friday, September 20, 2013 2:01 PM
To: Cristi Rigazio; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] The Good Old Days...

Seems like no one has touched upon the fine art of knife sharpening. Sometimes 
I felt like an executioner sharpening my axe to the point of splitting a hair. 
Heaven forbid if you hit a staple. That part of the blade was no useless for 
days to weeks until the daily sharpening ground it out. Life is pretty good 
when you just pull out another blade from the plastic box.

Victor 

Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB 244
Ninth  Jefferson
Seattle, WA 98104
vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
=
Privileged, confidential or patient identifiable information may be contained 
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disseminate or otherwise use this transmission. Instead, please notify the 
sender by reply e-mail, and then destroy all copies of the message and any 
attachments.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, September 20, 2013 10:40 AM
To: Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] The Good Old Days...

Hear hear!  I agree and was just saying I love hearing the stories (although I 
am not young).  Thanks all for sharing these memories and lessons!

Sent from my iPhone

On Sep 20, 2013, at 10:13 AM, Davis, Cassie cda...@che-east.org wrote:

 I enjoy hearing sincere reminiscing...Even though us kids don't know how 
 good we have it, some of us enjoy having an old tech beside us on the 
 bench. I find weeding through the sarcasm can be profitable and in doing so 
 have learned so much. What the old techs did on a daily basis, we only did in 
 the practice lab and when the automated instruments and pre-made solutions 
 that we have come to rely on fail, experience is so very valuable. Only by 
 their blood, sweat and tears have we benefitted however, we have so far to 
 go, let's do it together.
 
 Cassandra Davis
 cda...@che-east.org
 302-575-8095
 
 
 
 
 
 Confidentiality Notice:
 This e-mail, including any attachments is the property of Catholic 
 Health East and is intended for the sole use of the intended 
 recipient(s).
 It may contain information that is privileged and confidential.  Any 
 unauthorized review, use, disclosure, or distribution is prohibited.
 If you are not the intended recipient, please delete this message, and 
 reply to the sender regarding the error in a separate email.
 
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RE: [Histonet] Oil red O

[Goins, Tresa]

May be the position of the active group on the benzene ring - ortho vs. 
meta vs. para ?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Monday, September 09, 2013 2:06 PM
Cc: HISTONET
Subject: Re: [Histonet] Oil red O

Rene,
You still have not answered the question - what does the O stand for?




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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[Histonet] RE: VIP mix setting and use

[Goins, Tresa]

We use the SLOW exchange setting on all stations.  The FAST occurs twice as 
often and I didn't want the tissue specimens to be high and dry that many 
times during processing.  I want an Excelsior . . . . .  

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Tuesday, August 06, 2013 1:35 PM
To: Histonet
Subject: [Histonet] VIP mix setting and use

For those Histo Guru's who have the VIP tissue processors with the mix 
setting of Continuous, slow and fast, what is your perception of the benefit of 
using one over the another? What tissues would you use a particular setting? 
Why would you NOT use a particular setting?

I know the details of the setting timings, but the question came up as to why 
one would use continuous vs slow or fast, besides matching the timing to the 
reagent cycle time. Any insights will be appreciated!

Thanks!

Tim Morken
Pathology
UC San Francisco Medical Center

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[Histonet] RE: Slide dehydration After Staining Tissue Falling Off

[Goins, Tresa]

After the detergent treatment, rinse well in distilled water and dry in the 
oven.
Dip in clearant and coverslip.

We do not treat our developed IHC slides in alcohols or clearant due to 
potential loss of some labeling - not DAB.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher 
Jacobs
Sent: Monday, August 05, 2013 11:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide dehydration After Staining Tissue Falling Off

Histonetters,

Recently my lab has been experiencing a significant occurrence of tissue 
falling off our IHC slides. We are looking at a variety a factors and I have 
been watching how some technicians dehydrate their slides after staining. We 
are running Ventana IHC stainers, so we remove the oil based liquid coverslip 
in a weak solution of Dawn and then run the slides down alcohols into xylene. 
Some technicians are very aggressive in their agitation of the slides. It is 
almost comical how much sloshing around in the reagents they do. How can I get 
these people to ease up? Does anyone have any protocols on how many dips it 
takes in each reagent to properly dehydrate slides?

Also, some of the other issues we are looking at causing tissue falling off the 
slides are; tapping slides to remove water, not double-dipping slides, lotion 
on hands, over temperature, and the brand of charged slides we are using. Can 
anyone think of any other factors to look at?

Thanks!

CJ Christopher P. Jacobs, HT QIHC(ASCP)



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RE: [Histonet] Spirochete IHC on H-Pylori infected Tissue ?

[Goins, Tresa]

Belma -

Whether or not the spirochete antibody would work with H. pylori depends 
entirely on the anti-spirochete antibody specificity.
We use an anti-chlamydia antibody that hits all Chlamydia sp.; others are 
species specific.

The data sheet or the WEB site for the spirochete antibody should provide that 
information.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of a b
Sent: Wednesday, July 24, 2013 3:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Spirochete IHC on H-Pylori infected Tissue ?

Rene that sounded really rude, its a simple yes or no question. Some medical 
journals list H-Pylori as a Spirochete!
 that's why I asked in the first place... I wonder what your supervisor thinks 
of your knowledge,  especially when you spelled H-Pylori   H-pilori ??? hmm. 
-__-

On Sun, Jul 21, 2013 at 3:11 PM, Rene J Buesa 
rjbu...@yahoo.commailto:rjbu...@yahoo.com wrote:

 You are overlooking the essential characteristic of IHC, namely,
 specificity.
 Ab to H-Pilory will react only with H-Pilory, and the same goes for
 Spirochete.
 You will be wasting all your reagents and, worse of everything, your
 supervisor will wonder about your knowledge.
 René J.

   *From:* a b zipper...@gmail.commailto:zipper...@gmail.com
 *To:* 
 histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
 *Sent:* Saturday, July 20, 2013 10:09 PM
 *Subject:* [Histonet] Spirochete IHC on H-Pylori infected Tissue ?

 So here I am working my Saturday night, running an Immuno for Monday,
 only to open the fridge and find no more H-Pylori antibody cause we're
 out! : ( Can I substitute with Spirochete Antigen, and If so will the
 result will be as strong?

 As a standard procedure, I am doing a Warthin Starry on it also,

 Advice would be great. Thanks

 Belma Woodcraft ( Histology Lead )
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RE: [Histonet] microtome aligner

[Goins, Tresa]

Yikes.  There is a lot of play getting that bubble in the middle of the 
universal aligner that may translate to large variations in alignment each 
time the thing is used.  The high cost is probably for the clamp, but if the 
bubble level is worth $1.50 the quality of the clamp doesn't matter.

Save your money and use a blank paraffin block.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Mejia
Sent: Wednesday, July 17, 2013 8:12 AM
To: HistoNet
Subject: [Histonet] microtome aligner

Hello,

Can anyone provide any additional information regarding microtome aligners?
Do they work?  I have never used or seen this particular tool, so which brand 
works best?  I know of only one company that sells a microtome aligner 
(universal type).
Any information any one can provide will be most appreciated.

Best
Maria Mejia
San Francisco, CA


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RE: [Histonet] making 10 % EDTA

[Goins, Tresa]

The molecular weight of the EDTA will vary depending on chemical makeup - 
regardless, the solution you have is probably less than 10% already.  EDTA 
tri-sodium salt with MW 358 at 0.25 M is 89.5 g per liter or 8.95 g per 100 ml 
or 8.95%.  



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle
Sent: Wednesday, June 26, 2013 12:37 PM
To: histonet
Subject: [Histonet] making 10 % EDTA

Hi Histonetters,
 
I have purchased a liquid EDTA solution that is .25M. I need to make 10% EDTA.  
Do I just dilute it 1/10 with DI water?
 
 
Thanks
 
 
Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core University of Maryland Room NBW58, 
UMMC
22 S. Greene St
Baltimore, MD 21201
Pager#5029
(410) 328-5558
(410) 328-5508 fax
https://cf.umaryland.edu/freezer/promo_pbr.cfm
 

 

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confidentiality of this information. If the reader of this message is not the 
intended recipient; you are prohibited from using, disclosing, reproducing or 
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[Histonet] RE: Eosin not working for xylene free HE

[Goins, Tresa]

We use hot detergent solution for dewax and a xylene substitute (Pro-Par) after 
staining without problems.  If there is water accumulating in the clearant, it 
can be removed with a molecular sieve placed in the staining trays.  We don't 
have that problem here in Montana because it is dry dry dry.

Tresa 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Wednesday, March 27, 2013 8:11 AM
To: Gerard Spoelstra; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Eosin not working for xylene free HE

This exact reason is how I convinced my lab to go back to xylene!!  I figure 
it's because there is water in your substitute.  Because you can't see if there 
is water in it (from the air moisture or wherever...) it becomes frusterating 
and annoying to have to change out to fresh solutions sometimes several times a 
day!!  
Good Luck!!  


Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna 
Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gerard Spoelstra
Sent: Wednesday, March 27, 2013 9:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin not working for xylene free HE

Hi everyone,

I have not had any success optimising the eosin counterstain in the absence of 
xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking 
sections through to this to absolute ethanol but then air drying , but it 
leaves blotches of eosin.  Using eosin 0.5% aqueous with a few drops of acetic 
acid doesn't appear to work, the keratin stains heavily but not elastin. After 
washing in water I leave for a short time in the oven then at room temperature 
for 5 minutes then coverslip with the dako atomatic cover slipper.   For all 
the laboratories that have gone xylene free, are you just putting up with less 
than optimal eosin staining?

Gerard Spoelstra
Murdoch University
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RE: [Histonet] standard protocol

[Goins, Tresa]

Ask the pharmaceutical company for detailed instructions, not vague references.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Histopatty
Sent: Thursday, February 14, 2013 9:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] standard protocol


We are getting instructions from a pharmaceutical company who needs slides, 
with instructions stating sections should be floated per standard protocol, 
xylene dipped from 3 minutes and dried for 10 minutes.  I'm reaching out to 
those of you who routinly deal with research protocols and wondering is the 
standard protocol the normal float, dri, melt?

Patricia Eneff
for 
Kay Pierce

Please respond to sharon.pie...@hcahealthcare.com if possilbe.  
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RE: [Histonet] interview

[Goins, Tresa]

After a tour and review of the work-day expectations, the candidate completes a 
simple math test (basically dilutions and percentage calculations) and then is 
asked to observe and repeat the processes of sectioning and coverslipping.  
Look for signs of disinterest (no questions - the wall of silence) and a lack 
of the manual dexterity required to make a good slide (if you are fearful every 
time their hands approach the microtome, that's not a good sign - routine can 
be learned but spatial awareness not so much).   

My cohort was hired with zero experience and not certified and she's fabulous!



Tresa Goins
Histopathology Section Supervisor
Montana Veterinary Diagnostic Lab





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Thursday, January 10, 2013 6:22 PM
To: Gale Limron; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] interview

(Quoted material taken from various ASCP BOC (Board of Certification)
webpages.)

FIRST: make certain they meet the ASCP HT criteria. If it they are truly doing 
the OJT route:
Route 2: At least 60 semester hours (90 quarter hours) of academic credit from 
a regionally accredited college/university, with a combination of 12 semester 
hours (18 quarter hours) of biology and chemistry (must include credit hours in 
both), or an associate degree from a regionally accredited college/university, 
with a combination of 12 semester hours (18 quarter
hours) of biology and chemistry(must include credit hours in both), AND one 
year full time acceptable experience in a histopathology (clinical, veterinary, 
industry or research) laboratory in the U.S., Canada or an accredited 
laboratory* within the last ten years.
*Laboratory accredited by a CMS approved accreditation organization (i.e., 
AABB, CAP, COLA, DNV, The Joint Commission, etc.). NOTE: FOR U.S. 
CERTIFICATION THE JOINT COMMISSION INTERNATIONAL (JCI) IS NOT ACCEPTABLE.

SECOND: you also say they are willing to do an online training. If that is 
through a NAACLS-accredited HT program, then the ASCP HT criteria is:
Route 1: Successful completion of a NAACLS accredited Histotechnician program 
within the last 5 years prior to the date of application for examination;

So, you need to contact each of the NAACLS programs that offer on-line programs 
(there's 4-6 of them), and find out what their requirements are. 
High school graduates, some college, so many college credits, what type  and 
number of biology, chemistry and math requirements, etc.

Then you need to make it very clear who is paying (them, the lab, some of
both) the thousands of dollars of tuition, buying the books, how much time you 
will give them each week to work on homework projects (collecting tissue, doing 
stains, you monitoring them taking exams, etc.). I would have them sign a 
contract about being a trainee and earning less money than the minimum starting 
wage until they pass the ASCP HT exam, and then they get a raise to the 
minimum. And in the contract, if your lab it helping to pay for the tuition or 
book, that they agree to stay at least, say, 2 years after passing the HT exam, 
or else they have to pay the lab back some of the money the lab spent on 
training them (prorated, to amount of time they stayed past the time they 
passed the ASCP exam).

THIRD: If this is a true OJT, notice the one year full time acceptable 
experience. You say this is a part-time position, so 1 year of part time does 
not equal one year full time. This following is from the ASCP BOC webpage.
Full-time experience is defined as a minimum of thirty-five (35) hours per 
week. Individuals who have part-time experience may be permitted to utilize 
prorated part-time experience to meet the work experience requirements. For 
example, if you are employed 20 hours per week for one year, your experience 
would be computed as 20 divided by 35 multiplied by 52 weeks, or the equivalent 
of 29.7 weeks of full time employment.

My concern is your requirement of them taking and passing the ASCP HT exam in 2 
years. It might take 2 years for them to earn enough working hours to equal 1 
year full time experience. I would suggest that you tell them they must take 
and pass the HT ASCP exam within 1 year of becoming eligible. If they fail, 
they can take the exam again in the next quarter (4x/year). If they haven't 
passed it after 4 attempts, odd are they are not going to pass it. Or if they 
bother trying again every 3 months, well, that says something about their 
character also.

FOURTH: for your interview questions
- open questions. No yes/no.
- tell me about a time you . . .  are great questions. Anyone can make up 
something that sounds good if they are asked what would you do if . . .. 
But asking them to talk about a time when they had to handle a situation gives 
you an insight into what they did, why, and what they learned from it. 
And you can keep 

RE: [Histonet] (no subject)

[Goins, Tresa]

We recently switched to ion exchange resin with great results:

American MasterTech
I.E.D. Unit (Immuno) - Ion-Exchange Decalcification Unit 
Items: DCIEDIM60CS, DCIEDIM250CS, DCIEDIM250EA.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vikrant Piprode
Sent: Thursday, December 20, 2012 10:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

hi 
i want to know the best method to decalcify calvarial bone of mice for 
subsequent osteoclast staining and goldner's trichrome staining.
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RE: [Histonet] HE Question

[Goins, Tresa]

Jackie -

Try a different hematoxylin - we switched to Platinum Line Modified Harris 
Hematoxylin - we save money and the pathologists prefer the results.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts
Sent: Wednesday, November 14, 2012 8:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HE Question

Hi All,
I am having a problem with my staining, ( well, I think its pretty good but the 
path says its not cark enough for her) So, I am using Azer Scientific 
Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 
5  min and then to 10 and 12 min. The problem is the control I have works and 
looks great at the 2.5 min. Besides just keeping it in the heme longer what 
else can I do? I have cut a few extra dummy slides today so I can try different 
things out with them. I am tired of hearing from the path everyday that the 
heme is too light for her.
I am a new lab for a derm clinic and I work alone so its basiclly up to me to 
figure this out. Please help! Thanks!

Jackie
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[Histonet] RE: Control Block Log Sheets

[Goins, Tresa]

We write the accession number on the control block itself and transfer that ID 
to the slide label and to the data entry system when the control block is used. 
 This creates a permanent record while eliminating the need for a log that 
continually needs updating.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar
Sent: Wednesday, November 14, 2012 12:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control Block Log Sheets

Does anyone have a template they would be willing to share on how to keep track 
of control blocks?  Do you all use a type of log sheet to keep track, do you 
all number them a certain way to keep track?



Thank you

Fawn
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[Histonet] RE: Manual IHC for Melan-A

[Goins, Tresa]

We do manual staining for everything - here is the abbreviated version for 
Melan A -

Thermo Scientific MS799
Clone A103; Mouse MAb Isotype IgG1

Protocol:

1.  Dewax and hydrate sections
2.  Retrieve with HIER - EDTA pH 8 20 min  
3.  Block with NGS for 15 min
4.  Treat with primary Ab for 30 min
5.  Apply Link #1 - biotinylated α-mouse
6.  Apply Link #2 - streptavidin enzyme complex
7.  Rinse with water and apply chromogen 
8.  Rinse with dH2O 
9.  Counterstain, dry and coverslip

Results:
Staining is cytoplasmic
Granular cytoplasmic staining of adrenal cortical cells; minimal or no 
staining of kidney
Equivalent results using canine and feline tissue during validation

Tresa



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle M. 
Mackinnon
Sent: Thursday, November 08, 2012 9:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Manual IHC for Melan-A

Has anyone done manual IHC for Melan-A?  If yes, who help set up the manual 
protocol?
Thanks,
Michelle

Michelle M MacKinnon HT, (ASCP)
Histology Supervisor
Dermatology Consultants. PA
1215 Town Centre Drive, Suite 200
Eagan, MN 55123
Phone: 651-255-3462
Fax: 651-255-3461

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RE: [Histonet] Control tissue

[Goins, Tresa]

We put the accession number of the control tissue used on the slide and the 
information is also entered into our database.  This eliminates the need for 
keeping an additional separate record for control tissue identification.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter
Sent: Friday, November 02, 2012 10:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control tissue

Hi,
Can anyone tell me if the control blocks we use for special stains and IHC need 
to have the accession number of the original case they came from on them?  Does 
this info have to be put on the slides?  Are there any CAP regulations about 
this?  Thank you so much for your help.
Deloris Carter HT(ASCP)
Shawnee Mission Medical Center
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[Histonet] RE: labeling pre-made slides

[Goins, Tresa]

We have no label maker - so the slides are labeled with a Securline Marker II 
from EMS - stays put through all treatments.
Prior to delivery, a paper label is printed and affixed to the slide over the 
hand-written label.

Tresa 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Thursday, October 18, 2012 12:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] labeling pre-made slides

We use the Leica IPS to label our cytology slides, however, we do get quite a 
few slides from doctors who make smears and spray fix them before they come to 
us.  Unfortunately, they use pencil to label and this usually wipes off during 
staining and coverslipping.  Does anyone else receives slides like this and 
then how are they permanently labeled?  We would like to avoid going back to 
paper labels if at all possible.
Any advice or suggestion is appreciated
Thanks
Allison
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[Histonet] RE: labeling pre-made slides

[Goins, Tresa]

I don't know if you tried these markers specifically, but we have seen 
variation in quality.  We changed vendors because we began having problems with 
our original source - the vendor changed their supplier and tried selling the 
pen as the same as - it was not even close.  

Also, you may try cleaning the frosted area with acetone before labeling.

Tresa

-Original Message-
From: Hutton, Allison [mailto:ahut...@dh.org] 
Sent: Thursday, October 18, 2012 1:39 PM
To: Goins, Tresa; histonet@lists.utsouthwestern.edu
Subject: RE: labeling pre-made slides

Some further clarification...
*We manually coverslip
*We have tried the markers upon reciept but have trouble with them staying on 
due to the spray fixative ending up on the frosted end as well.

-Original Message-
From: Goins, Tresa [mailto:tgo...@mt.gov]
Sent: Thursday, October 18, 2012 3:08 PM
To: Hutton, Allison; histonet@lists.utsouthwestern.edu
Subject: RE: labeling pre-made slides


We have no label maker - so the slides are labeled with a Securline Marker II 
from EMS - stays put through all treatments.
Prior to delivery, a paper label is printed and affixed to the slide over the 
hand-written label.

Tresa 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Thursday, October 18, 2012 12:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] labeling pre-made slides

We use the Leica IPS to label our cytology slides, however, we do get quite a 
few slides from doctors who make smears and spray fix them before they come to 
us.  Unfortunately, they use pencil to label and this usually wipes off during 
staining and coverslipping.  Does anyone else receives slides like this and 
then how are they permanently labeled?  We would like to avoid going back to 
paper labels if at all possible.
Any advice or suggestion is appreciated
Thanks
Allison
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RE: [Histonet] RE: Metal molds

[Goins, Tresa]

I knew I might catch some grief for not cleaning our molds for five years, but 
my workplace is clean and organized.

Some things just work better without being cleaned with soap and water - I have 
a fifteen year old cast iron skillet that is as non-stick as Teflon due to a 
natural patina - maybe the paraffin blocks easy release from my un-clean 
molds” works for the same reason.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brendal Finlay
Sent: Wednesday, October 10, 2012 8:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


We clean molds every day.  My preferred method is heated water to melt the 
paraffin off, then allow to cool.  Peel the paraffin from the surface of the 
water, remove the molds from the water, then dunk them about 10 times in a 
mixture of alcohol and mold release.  Allow to air dry or dry in a low temp 
oven.


In my experience, molds that aren't cleaned on a regular basis make it very 
difficult to remove the embedded cassettes even if very, very cold.  It's 
easier for me if my workspace and tools are clean and organized.

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RE: [Histonet] RE: Metal molds

[Goins, Tresa]

Dag' nabbit'  Now I'm hungry . . . 

Tresa

-Original Message-
From: Blazek, Linda [mailto:lbla...@digestivespecialists.com] 
Sent: Wednesday, October 10, 2012 11:38 AM
To: 'Bartlett, Jeanine (CDC/OID/NCEZID)'; Fred Underwood; 
histonet@lists.utsouthwestern.edu; Brendal Finlay; Goins, Tresa
Subject: RE: [Histonet] RE: Metal molds

Ok  that did it...  I'm going home and making cornbread, hash browns with 
onions, sausage and green pepper.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCEZID)
Sent: Wednesday, October 10, 2012 1:30 PM
To: Fred Underwood; histonet@lists.utsouthwestern.edu; Brendal Finlay; Tresa 
Goins
Subject: RE: [Histonet] RE: Metal molds

I'm thinking some cracklin cornbread!

Jeanine H. Bartlett
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood
Sent: Wednesday, October 10, 2012 1:29 PM
To: histonet@lists.utsouthwestern.edu; Brendal Finlay; Tresa Goins
Subject: RE: [Histonet] RE: Metal molds

You're making me hungry Tresa.  I bet that skillet makes a killer batch of hash 
browns.

 Goins, Tresa tgo...@mt.gov 10/10/2012 12:53 PM 
I knew I might catch some grief for not cleaning our molds for five years, but 
my workplace is clean and organized.

Some things just work better without being cleaned with soap and water
- I have a fifteen year old cast iron skillet that is as non-stick as Teflon 
due to a natural patina - maybe the paraffin blocks easy release from my 
un-clean molds* works for the same reason.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brendal Finlay
Sent: Wednesday, October 10, 2012 8:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


We clean molds every day.  My preferred method is heated water to melt the 
paraffin off, then allow to cool.  Peel the paraffin from the surface of the 
water, remove the molds from the water, then dunk them about 10 times in a 
mixture of alcohol and mold release.  Allow to air dry or dry in a low temp 
oven.


In my experience, molds that aren't cleaned on a regular basis make it very 
difficult to remove the embedded cassettes even if very, very cold.
 It's easier for me if my workspace and tools are clean and organized.


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[Histonet] RE: Metal molds

[Goins, Tresa]

We haven't cleaned our molds for five years - if the blocks are cold enough 
after embedding, there is no problem removing the block.  


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Monday, October 08, 2012 2:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Metal molds


 OK folks, I know I should be smarter than this and I haven't seen discussion 
on it lately 

Are people cleaning their metal embedding molds after evey embedding session?

If not, how often do you clean them? 

Do you clean them at all?

If you clean them, how do you do it? 

Thanks

Bill
William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan 
Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.

Cultivate it in PRAYER!

 


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named addressee(s) and contain confidential information. If you are not an 
addressee, or responsible for delivering this email to an addressee, you have 
received this email in error and are notified that reading, copying, or 
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[Histonet] RE: CK AE1/AE3

[Goins, Tresa]

We (veterinary lab) use Pan Cytokeratin Plus [AE1/AE3+8/18] from Biocare.  
Enzymatic retrieval will yield positive results for skin from all species 
tested but HIER using EDTA pH 8 is required for positive results on 
adenocarcinomas and squamous cell carcinomas.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean
Sent: Wednesday, October 03, 2012 1:47 PM
To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] CK AE1/AE3 

Hi Everyone,

I'd like some input as to what labs are using as a procedure for this antibody. 
Is enzyme digestion or a form of antigen retrieval with heat used? What 
dilution and detection system do you use as well as what type of machine is it 
run on?

Thanks for the info,

Jean Taylor, HT (ASCP) QIHC
IHC Tech
Meriter Health Services
Madison, WI
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[Histonet] Picric Acid

[Goins, Tresa]

A WEB site just for historical interest: 
http://en.wikipedia.org/wiki/Halifax_Explosion 

We continue to use picric acid in the lab, but only as an aqueous or saturated 
solution.

The chemical safety guys came out and carefully removed the bottle of 
moistened picric acid that we had on the shelf for several years - they were 
very excited as it was no longer moist - Montana is very dry.

Tresa


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire 
Sent: Wednesday, September 19, 2012 1:17 PM
To: Geoff; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards

Yes, but why take the chance. There are also other chemicals in the lab the 
picric acid can interact with to make it even more volitile than it was to 
begin with. Dynamite other explosives have the same problem. The older it gets 
the more degraded and unstable it becomes. One never knows if or when. I'd like 
to avoid traumatically amputating my arms if possible, thank you.
Claire



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Geoff
Sent: Mon 9/17/2012 9:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards



I am with Wayne on this one. While I have not tried to make it explode it does 
seem to me that the dangers are hyped beyond reason.
Years ago an old bottle of picric acid would be discovered in a high school 
chemistry lab. Horrors! Call the bomb squad! So it was taken out to a large 
field, packed with explosives and BOOM! Of course it exploded, it was 
surrounded with explosives.

Geoff

On 9/14/2012 8:58 PM, E. Wayne Johnson wrote:
 What danger of Picric Acid are you concerned with?




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[Histonet] RE: Gloves

[Goins, Tresa]

We use nitrile when handling everything except acetone - then we use latex.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richardson, Pam 
K
Sent: Tuesday, August 21, 2012 1:53 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Gloves

Hi, what type of gloves are you wearing when handling xylene? Do you double 
glove?

Cordially,

Pam ~


Pam Richardson
Clinical Manager
Gundersen Lutheran Laboratory 
Email: pkric...@gundluth.org
Phone: 608 775-4133
Fax: 608 775-6136
Interdepartmental Mail Stop: H04-007
E-visit us at: http://www.gundluth.org

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[Histonet] RE: Proteolytic enzyme pretreatment before immunostaining

[Goins, Tresa]

The proteolytic activity of the enzymes can be non-specific (Pronase) or 
specific (peptide bonds at lysine and arginine residues, Trypsin).  The effect 
of formalin fixation is not identified, so we always assess Pepsin, Trypsin and 
Pronase along with heat retrieval methods regardless of what the supplier data 
sheet recommends.

Tresa  



Dear Histonetters,

Could anyone explain about the difference between proteolytic enzymes for 
immuostaining? 

We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use 
one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did 
manual staining.

At the moment I am using Leica's BondMax autostainer and their enzyme 
pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the 
pretreatment condition would be affected by the concentration of enzymes, pH, 
temperature, incubation time etc.

 

My question is that do they have different mode of action on tissues? I am 
helping a research project and our antibody includes various clones of Integrin 
6 subunit and uPAR.

I have tried enzyme pretreatment with autostainer and manual staining with 
Proteinase K (Dako). It seems that some antibodies work better with certain 
enzymes. 

I mean that some antibodies work well after BondMax enzyme treatment, but some 
antibody works better with proteinase K pretreatment manually.
I am using the same polymer detection system (Leica Microsystem) for both 
methods.

I would like to find an enzyme which works for both of our antibodies at the 
same time.

Thank you.

 

 

 

Young Kwun

Senior Hospital Scientist

Immunohistochemistry

Dept. of Anatomical Pathology

Concord Repatriation General Hospital

Concord NSW 2139 Australia

 

02-9767-6075 (Tel)

02-9767-8427 (Fax)

young.k...@sswahs.nsw.gov.au

 

 

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RE: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining

[Goins, Tresa]

When re-reading my e-mail, it does sound like we use heat and enzymatic 
retrieval together - we do not - we assess the effective of all retrieval 
methods used singly.

Tresa

-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Wednesday, August 01, 2012 10:28 AM
To: Goins, Tresa
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] RE: Proteolytic enzyme pretreatment before 
immunostaining

I think formalin fixation does something similar to enzyme-binding as to 
antibody-binding, if the enzyme has a specific binding site. The aminoacids are 
masked by methylol-adducts and therefore the protein is protected against eg 
pepsin.
So using combinated HIER and PIER is not only summarizing the effects. HIER 
renders PIER more effective and therefore more aggressive.

Gudrun Lang

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Goins, Tresa
Gesendet: Mittwoch, 01. August 2012 16:00
An: Young Kwun; histonet@lists.utsouthwestern.edu
Betreff: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining

The proteolytic activity of the enzymes can be non-specific (Pronase) or 
specific (peptide bonds at lysine and arginine residues, Trypsin).  The effect 
of formalin fixation is not identified, so we always assess Pepsin, Trypsin and 
Pronase along with heat retrieval methods regardless of what the
supplier data sheet recommends.

Tresa  



Dear Histonetters,

Could anyone explain about the difference between proteolytic enzymes for 
immuostaining? 

We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use 
one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did 
manual staining.

At the moment I am using Leica's BondMax autostainer and their enzyme 
pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the 
pretreatment condition would be affected by the concentration of enzymes, pH, 
temperature, incubation time etc.


 

My question is that do they have different mode of action on tissues? I am 
helping a research project and our antibody includes various clones of Integrin 
6 subunit and uPAR.

I have tried enzyme pretreatment with autostainer and manual staining with 
Proteinase K (Dako). It seems that some antibodies work better with certain 
enzymes. 

I mean that some antibodies work well after BondMax enzyme treatment, but some 
antibody works better with proteinase K pretreatment manually.
I am using the same polymer detection system (Leica Microsystem) for both 
methods.

I would like to find an enzyme which works for both of our antibodies at the 
same time.

Thank you.

 

 

 

Young Kwun

Senior Hospital Scientist

Immunohistochemistry

Dept. of Anatomical Pathology

Concord Repatriation General Hospital

Concord NSW 2139 Australia

 

02-9767-6075 (Tel)

02-9767-8427 (Fax)

young.k...@sswahs.nsw.gov.au

 

 

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[Histonet] RE: Hold IHC overnight

[Goins, Tresa]

I vote No - this run would be valid only if you held all future slides 
overnight after HIER.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Wednesday, July 18, 2012 11:44 AM
To: histonet
Subject: [Histonet] Hold IHC overnight

Just found out I have to go to a meeting in 2 hours for the rest of the day (I 
haven't even eaten lunch yet).  I do IHC manually, they just got done cooling 
off from HIER...I can store them overnight in buffer right?
Just double checking myself as this run is an antibody validation run...
-S-

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna 
Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

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[Histonet] Hydrometer temperature correction

[Goins, Tresa]

There is a chart [ http://www.defalcos.com/tutorials/hy.html ] for temperature 
correction on ethanol concentration.  A lot of information can be found on 
brewery sites.  So . . if you require more help, and since its Friday, visit a 
local brew house to ask questions.  

Tresa :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Friday, June 29, 2012 8:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hydrometer questions

Hi everyone:

I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue 
problem.

First, I am going to compare using a very old LFB bottle (from the
1950s) and a new bottle.
Then I will stain some test slides for 2 hours and 24 hours at 60C.
(I am having my oven checked this morning by facilities).


To find out how much water is really in my 95% ethanol, I am assuming 
that I need a hydrometer.
(I will also be using it to measure the isopropanol concentrations on my 
tissue processor).

Is there a type of hydrometer that labs use for these purposes?
Where do you buy yours from?
Can the alcohol/water concentrations be read right off of the 
hydrometer, like temperature is from a thermometer, or do you need to 
use an equation.
I have never used a hydrometer before.

Thank you,

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA
617-855-3592



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[Histonet] RE: Antigen retrieval

[Goins, Tresa]

Mike,

A good retrieval method will restore the epitope to a configuration that the 
antibody will recognize.  The data sheet received with a commercial antibody 
may suggest a retrieval method to use, but if you are using a non-commercial 
source, trying a variety of methods may help unless the epitope is irreversibly 
altered by fixation.

We run all new assays using pH 9, pH 6 and sometimes pH 3 [10 min treatment 
rather than 20 min] in addition to enzymatic retrieval using Pepsin, Trypsin or 
Protease and finally No Retrieval.  

Happy Hunting,

Tresa
___

Does anyone have a favorite antigen retrieval method for FFPE mouse tissues 
that they would be willing to share? I have been using citrate buffer Ph6.0 
with poor to moderate results. Thanks for any help!



Mike
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RE: [Histonet] Reagent rotation

[Goins, Tresa]

We rotate by cassette number - our daily load can vary from 10 to more than 100 
cassettes, so an exchange schedule based on number of runs was not appropriate.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Perrin
Sent: Tuesday, June 05, 2012 12:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reagent rotation

What is the normal procedure for changing the reagents in the tissue processor? 
We have a Sakura VIP6. Should the schedule of changing be based on number of 
runs vs. number of blocks processed? About 80% of our blocks are small GI 
biospies.

Thanks in advance for your input.

 
Toshia Perrin
Medical Practice Coordinator
Southern Pathology

This message contains privileged and confidential information intended only for 
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[Histonet] RE: Histonet Digest, Vol 102, Issue 40

[Goins, Tresa]

I personally found the discussions on certification enlightening and 
occasionally entertaining.  
The opinions expressed are simply opinions - I do not judge them as nasty or 
tedious.  

If I am not interested in a discussion thread, I simply do not read it. 

tg
  


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Freeman, Carol
Sent: Friday, June 01, 2012 7:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 102, Issue 40

I find it sad that there are so many messages to unsubscribe following the 
great certification debacle that has been on here the last couple of days.  I 
personally just find it tedious that on a server set up purely for histology is 
where we are discussing whether or not histology is a respectable profession.  
I would like to believe it is the ones sharing their nasty viewpoints and lack 
of self worth, but sadly it is probably some of the good ones that we have 
lost.  




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[Histonet] RE: Monoclonal antibodies from different companies

[Goins, Tresa]

In my experience, identical clones from different companies are not always 
equivalent.  

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson
Sent: Tuesday, May 22, 2012 9:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Monoclonal antibodies from different companies

Dear Histonetters,

If you can get a monoclonal antibody from different sources, the same clone, 
the same Ig concentration, is it the same? In other words, we are using clone 
#XX#X from Company A. Someone else has had success using clone #XX#X from 
Company B.

Is there any benefit to buying the antibody from Company B to try, or would it 
be a duplication of effort and cost?

Thanks for your input!

Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752

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RE: [Histonet] paraffin melting in VIP

[Goins, Tresa]

When it is time to exchange reagents, the paraffin is the first thing we do 
because of the melting time required.
We fill up the bin and place it in the oven until there is room to add more wax 
pellets - we do not stuff it in, but leave it rather loose.  We do this about 
six times during the day until the bin is full.
The paraffin pellets are not cold and there is a lot of residual heat in the 
oven due to the three hot wax bins already at temperature, so I am not sure how 
much stress this places on the heating elements.  

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Friday, May 11, 2012 11:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] paraffin melting in VIP

HI!

A question for those, who melt the paraffin directly in the VIP. How long does 
it take to melt the pellets in the VIP-oven?

 

Thanks

Gudrun

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[Histonet] RE: Unregistered HT testing

[Goins, Tresa]

I disagree with your assessment of complex staining.  IHC staining is like 
cookie-cutter staining - one does the same steps every single time with a 
different (but very similar) set of reagents.  The quality of special stains on 
the other hand are determined by a unique chemistry - one can get it wrong in 
so many ways.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole
Sent: Tuesday, April 17, 2012 1:37 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Unregistered HT testing

Hello everyone!

I'm just curious to know if anyone is allowing unregistered HT's to do special 
stains in their CAP accredited lab?  I have been involved in discussions 
regarding high complexity testing.  From the feedback I have received, special 
stains and IHC stains are considered high complexity testing.  I beg to 
disagree.  I can understand IHC/ISH as high complexity but I don't think 
routine special stains fall under that category.  I'd appreciate any feedback 
or literature you can reference for me to review.  Thank you!

Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department



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RE: [Histonet] Thank you

[Goins, Tresa]

We used Fisher Scientific cassettes for years without a problem.  Then we had a 
big problem; smudged ink and failure to close.  The writing surface was slick, 
not textured, there was not enough plastic injected into the mold to form the 
tabs on the locking lid and a plastic bleed between the locking tab and the 
cassette body prevented full closure of the cassette.

I sent some samples to Fisher because they were interested in seeing the 
problem.  I didn't hear boo back.  I switched suppliers.  I'm happy again.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Thursday, April 12, 2012 7:44 AM
To: Rathborne, Toni; 'Louise Renton'; Histonet
Subject: RE: [Histonet] Thank you

We have had the same problems as everyone else,  i.e. pencil or markers on 
cassettes.  What I am seeing is that the cassettes have changed.  They are not 
textured on the writing edge as before.  The edge is smooth and the pencil 
smudges.  Some markers wash off.  The problem here is alcohol, not xylene 
(substitutes) or formalin.  Also KP Markers did have a bad batch that washed 
off as well.  But as someone else commented, that too has been fixed.  We use 
the KP Markers and have had no problems.  There also was a batch of Fisher 
cassettes that where so smooth the ink/pencil faded to almost non-existent.  We 
test every new marker before it is put into use in alcohol.  If it bleeds we 
rub it to see if the numbers are still legible.  




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Thursday, April 12, 2012 9:05 AM
To: 'Louise Renton'; Histonet
Subject: RE: [Histonet] Thank you

Not necessarily true. We used pencils for years and are now trying different 
markers because the pencil is smudging.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Renton
Sent: Thursday, April 12, 2012 3:11 AM
To: Histonet
Subject: Re: [Histonet] Thank you

there's always good old pencil (HB) as a backup

On Thu, Apr 12, 2012 at 12:05 AM, Patsy Ruegg pru...@ihctech.net wrote:

 The best markers I have ever used are called KP Markers, they were off 
 the market for a while, but they are back and we get them from 
 Mercedes Medical, just got a new batch and they are wonderful just 
 like the old KP markers, we won't have anything else in the lab.

 Patsy Ruegg, HT(ASCP)QIHC
 IHCtech
 12635 Montview Blvd. Ste.215
 Aurora, CO 80045
 720-859-4060
 fax 720-859-4110
 www.ihctech.net
 www.ihcrg.org


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim 
 Wheelock
 Sent: Wednesday, April 11, 2012 2:43 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Thank you

 Hi Everyone:

 I want to thank everyone who gave me advice concerning my cassette 
 labeling problem.
 I am trying out three different types of markers pens: StatLab, Leica, 
 and NewComer Supply Histo-tec brands.
 We will also make sure to let the ink dry before putting the cassettes 
 into formalin.
 If we still have a problem, we will experiment with a different cassette.
 Thanks again.

 Tim Wheelock
 Harvard Brain Bank
 McLean Hospital
 Belmont, MA
 617-855-3592

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--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel  fax)
073 5574456 (emergencies only)
Question: Are rhinos  overweight unicorns?
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RE: [Histonet] H E Staining

[Goins, Tresa]

We use Platinum Line hematoxylin and eosin from Mercedes Medical [MER4799 and 
MER4131] - much cheaper than Richard-Allan and in a side-by-side blind test, 
the pathologists actually preferred Platinum Line.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Tuesday, April 03, 2012 7:51 AM
To: Histonet
Subject: [Histonet] H  E Staining

Good morning Histonetters,
 
Can anyone tell me if you are using a particular company's reagents for your 
HE staining that are pre-made and meant to work together?  Also, is the cost 
significantly more than buying separate reagents and making up your own?
 
Thanks,
Sheila, HT (ASCP)
Knoxville, TN
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RE: [Histonet] Need the name of a piece of equipment

[Goins, Tresa]

Found a wax trimmer from Ted Pella for less than $300

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles
Sent: Thursday, March 29, 2012 10:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Need the name of a piece of equipment

Good day to all,
I need the name of the equipment that melts the wax off the edges of a paraffin 
block.
Where can I purchase it.

Thank You
Stella
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[Histonet] RE: Antiboey Diluents

[Goins, Tresa]

Not all antibody diluents contain BSA - proprietary carrier proteins include 
synthetic polymers.  
Not all antibody diluents are buffered with PBS - some are buffered with TBS.
If you make your antibody diluents in-house, be very selective about the BSA 
source, especially if you work with veterinary tissues.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, March 21, 2012 2:43 PM
To: histonet@lists.utsouthwestern.edu; Joseph Madary
Subject: Re: [Histonet] commercial antibody diluent with carrier proteins 
SUGGESTIONS?

Joseph:
ALL commercial antibody diluent carriers  contain the same ingredients (bovine 
albumen, Tween 20 and sodium azide as preservative in pH7 PBS buffer).
 
Every 100 mL of antibody diluent contains:
Bovine albumen - 1 mL
PBS pH7 buffer - 99 mL
Tween 20 - 50 µL
sodium azide --100 mg
 
Why don't you prepare it yourself? That is what I used to do and saved a lot of 
money!
René J.

--- On Wed, 3/21/12, Joseph Madary pat...@gwumc.edu wrote:


From: Joseph Madary pat...@gwumc.edu
Subject: [Histonet] commercial antibody diluent with carrier proteins 
SUGGESTIONS?
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, March 21, 2012, 11:27 AM


Hi all, I would like to get a commercially prepared antibody diluent with 
carrier proteins. I have been told Dako has a decent one but I am trying to 
find a different vendor. I see a few places with diluent but none that have 
background reducing substances/carrier protein type thing. 


Nick Madary, HT/HTL(ASCP)QIHC
George Washington University
Pathology Core Laboratory
Ross Hall, Room 706
23rd and I Street NW
Washington D.C. 20037
202.994.8916
pat...@gwumc.edu

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RE: [Histonet] Control Block Tracking

[Goins, Tresa]

I must disagree  - at one time we also kept a control block list - it can very 
easily become a record keeping nightmare.
We write the ID of the control tissue used in the data base and on the slide 
label - end of story.  No control block written records to update, file, access 
or archive.

My four cents,


Tresa Goins
Montana Veterinary Diagnostic Lab
Bozeman, Montana 59718

406-994-6353



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, March 06, 2012 9:32 AM
To: histonet@lists.utsouthwestern.edu; SaraBreeden
Subject: Re: [Histonet] Control Block Tracking

Absolutely unnecessary! You have to remember that an inspector, in order to 
appear knowledgeable and demanding, has to say something, even if it is 
stupid.
But you can comply in a simple way (as I used to do):  make a list of your 
control blocks, with documentation about what antigens they are used for. Also 
write the dates from/to that particular block was used.
If you want to know what control was used for a specific case/antigen, just 
check the data the test was performed, and refer to your control blocks list to 
know which was use according with the date.
The problem arises when you use, for example normal tonsils, although those can 
also be referred to a case number (the one corresponding to the tonsil 
resection).
In this way you will comply without the need to add additional information to 
every case.
René J.

--- On Tue, 3/6/12, Breeden, Sara sbree...@nmda.nmsu.edu wrote:


From: Breeden, Sara sbree...@nmda.nmsu.edu
Subject: [Histonet] Control Block Tracking
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, March 6, 2012, 9:42 AM


During a recent inspection of my department, the inspector encouraged
me to develop a method of tracking which control tissue I used for a case 
(i.e., individually identifying the block and referencing that identifier on 
the slide).  To be honest, I believe this to be overload, but since I'm 
probably going to have to abide by that encouragement, I'd like to know 
if/how you identify which control block is used for a special stain.  I can 
think of a simple method but wonder if any of you have used a really 
spectacular method that just blew the inspecting agency away.  I'd promptly 
plagiarize it!  Gracias!



Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)



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[Histonet] RE: Congo red

[Goins, Tresa]

Cut at 8-10 microns - I believe birefringence depends on the increased 
thickness of the tissue.  We stain our sections with Congo Red for 5 min with 
good color and fluorescent results.


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Thursday, March 01, 2012 7:28 AM
To: histonet
Cc: histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Congo red

Anyone have any solution to a week Congo Red? I use Rowley  Congo red, 1% 
aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And 
I leave in the solution for up to 4 hours and the paths are still saying it is 
weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step 
and its still too weak.

Cheryl A. Miller HT(ASCP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554



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RE: [Histonet] Does xylene cause skin cancer?

[Goins, Tresa]

Nitrile gloves are recommended for all the chemicals we use in our lab except 
acetone - for acetone latex is recommended.
We also coverslip by hand but we wear nitrile gloves without exception.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega
Sent: Tuesday, February 21, 2012 8:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Does xylene cause skin cancer?

I am asking this because in my job we mount slides by hand, and my coworkers 
don't like to use gloves because it leaves a residue of latex in the back of 
the slides. I really don't feel comfortable mounting without gloves because I 
heard that xyelene can cause cancer. Some people I know personally has told me 
that this is not possible, but I read in some places that xylene could a 
possible carcinogen.

I have already gotten contact with xylene in my hands a couple of times and I 
am worried.



Thanks.
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RE: [Histonet] new billing

[Goins, Tresa]

Now I am confused.  Did you mean to state same antibody on multiple slides of 
the same specimen rather than multiple blocks of the same specimen?



I am asking because of questions from breast cancer patients who have 
difficulty getting coverage from their insurance companies when multiple lymph 
nodes are submitted from a single surgery.



Thanks,



Tresa



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Tuesday, February 14, 2012 8:36 AM
To: anita dudley; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] new billing



You can charge for each antibody on each specimen. You cannot charge for the 
same antibody on multiple blocks of the same specimen.



e.g. Sentinel node - Melan A, S100, HMB45

A1 - A4 = 3 charges not 12.



Hope this makes sense..



Joyce Weems

Pathology Manager

Saint Joseph's Hospital

5665 Peachtree Dunwoody Rd NE

Atlanta, GA 30342

678-843-7376 - Phone

678-843-7831 - Fax





-Original Message-

From: 
histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley

Sent: Tuesday, February 14, 2012 10:30

To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

Subject: [Histonet] new billing





can someone explain the new billing for the antibodies on blocks?  not sure 
what they mean.  can you just bill one antibody per block even tho

you may do three or four?   thanks so much

anita dudley

providence hosp

mobile alabama

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[Histonet] RE: picric acid

[Goins, Tresa]

The picric acid around the cap would not be 1%.  The acetone is long gone.  
Wipe the threads or pipette the reagent from the bottle.
Our chemical safety office informed us that friction from removing a cap can be 
enough to set it off.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret
Sent: Friday, February 03, 2012 11:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] picric acid

I am curious how big an explosion there would be from 1% picric acid in acetone 
if a little dried around the cap.

Margaret Perry HT(ASCP)
Dept of Veterinary and  Biomedical services Box 2175 South Dakota State 
University Brookings SD 57007
605-688-5638

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RE: [Histonet] RE: picric acid

[Goins, Tresa]

Geoff -

Yes - see if you can get the footage of the damage done in a lab in Idaho.  

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe
Sent: Friday, February 03, 2012 12:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: picric acid

Our chemical safety office informed us that friction from removing a cap can 
be enough to set it off.

I have heard this for many, many years but has there ever been a case of such a 
thing happening? Of course we should be cautions and keep our picric acid wet 
but ...
I have seen news clips in which the bomb squad packs explosives around the 
picric acid (from an old high school lab) out in a field and sets it off. BOOM! 
Sure, the picric acid was surrounded by explosives.

Geoff

On 2/3/2012 2:04 PM, Goins, Tresa wrote:
 The picric acid around the cap would not be 1%.  The acetone is long gone.  
 Wipe the threads or pipette the reagent from the bottle.
 Our chemical safety office informed us that friction from removing a cap can 
 be enough to set it off.

 Tresa

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, 
 Margaret
 Sent: Friday, February 03, 2012 11:51 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] picric acid

 I am curious how big an explosion there would be from 1% picric acid in 
 acetone if a little dried around the cap.

 Margaret Perry HT(ASCP)
 Dept of Veterinary and  Biomedical services Box 2175 South Dakota 
 State University Brookings SD 57007
 605-688-5638

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--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
**



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[Histonet] RE: reagents without expiration dates

[Goins, Tresa]

A general rule that I am familiar with is a maximum shelf-life of 1 year, 
basically due to repetitive access leading to eventual contamination.


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stoll, Kathryn
Sent: Thursday, February 02, 2012 11:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] reagents without expiration dates

Could anyone share a policy to deal with regents that do not have a 
manufacturer's expiration date?
CAP checklist ANP 21382

Thanks,
Kathryn Stoll, HT(ASCP)
Depatment of Pathology
Medical College of Wisconsin
9200 W Wisconsin Ave
Milwaukee WI 53226
414.805.1525
kst...@mcw.edu

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RE: [Histonet] reagents without expiration dates

[Goins, Tresa]

OK, I'll give it a try René.  Baker's Analyzed Potassium Permanganate (5 lbs) 
with a typed label made with an actual typewriter.  Trying to verify age 
but I've wasted enough time already on fun stuff today.



Tresa



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 02, 2012 2:15 PM
To: histonet@lists.utsouthwestern.edu; Emily Sours
Subject: Re: [Histonet] reagents without expiration dates



Ha.Ha,Ha!!!

I used to prepare staining solutions with some Merck-Darmstad anilines 
manufactured just after the Great War, i.e. the FIRST World War (about 1925 
before the World Great Depression).

Try to beat that!.

René J.



--- On Thu, 2/2/12, Emily Sours 
talulahg...@gmail.commailto:talulahg...@gmail.com wrote:





From: Emily Sours talulahg...@gmail.commailto:talulahg...@gmail.com

Subject: Re: [Histonet] reagents without expiration dates

To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

Date: Thursday, February 2, 2012, 3:33 PM





I've always wanted to have a contest to see who had the oldest reagents.

My lab once had something that was 20 years old.



Emily



The whole point of this country is if you want to eat garbage, balloon up to 
600 pounds and die of a heart attack at 43, you can! You are free to do so. To 
me, that’s beautiful.

--Ron Swanson







On Thu, Feb 2, 2012 at 2:07 PM, WILLIAM DESALVO 
wdesalvo@hotmail.commailto:wdesalvo@hotmail.comwrote:





 Per SOP, we relabel, list date of receipt, test for quality and then

 apply a 12 month expiration date. We re-test after 12 months and

 continue to use, with 12 month dating, as long as the reagent meets

 quality standards set in the SOP.



 William DeSalvo, B.S., HTL(ASCP)







  From: kst...@mcw.edumailto:kst...@mcw.edu

  To: 
  histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

  Date: Thu, 2 Feb 2012 12:56:58 -0600

  Subject: [Histonet] reagents without expiration dates

 

  Could anyone share a policy to deal with regents that do not have a

 manufacturer's expiration date?

  CAP checklist ANP 21382

 

  Thanks,

  Kathryn Stoll, HT(ASCP)

  Depatment of Pathology

  Medical College of Wisconsin

  9200 W Wisconsin Ave

  Milwaukee WI 53226

  414.805.1525

  kst...@mcw.edumailto:kst...@mcw.edu

 

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RE: [Histonet] Music in the Laboratory

[Goins, Tresa]

I got a pair of Bose speakers for the lab - I like to listen to quality sound 
while I work and we bring in ipods, phones, whatever.  But, everyone has the 
option of nixing any music selection at any time or opting for no music.  
Depends on the mood of the lab on any particular day. 

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tracz...@aol.com
Sent: Tuesday, January 17, 2012 7:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Music in the Laboratory

Greetings.
I would like to know what other histology laboratories allow for music  
players while working.  Do you have formal policies about music content or  
volume?  Do you allow lab space doors to remain closed to muffle the volume  of 
what is being played?  Are headsets allowed?  
I am a terrible judge of this because I personally prefer to work in a  
quiet environment.  I am trying to be open minded, as long as the work gets  
done.  However, one of the techs had a song playing today that I  believe was 
inappropriate for general listening in the lab.  Am I just out  of touch?  
Is that dang F word just something I'm going to have to learn  to accept?  
Do you have a written policy?  When/how/why was it  implemented?
I should mention that it's a small private lab, with minimal patient  
traffic.  We do see our share of FedEx, UPS, sales  service  reps.
Your ideas on this is very much appreciated.
Dorothy
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[Histonet] RE: lab chairs

[Goins, Tresa]

We use backless drafting stools made by Office Star ordered from www.cymax.com. 
The saddle seat gets funny looks but we wouldn't use anything else now - they 
roll, tilt, go up and down and eliminate the pressure on the thighs from the 
front edge of a regular chair.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Wednesday, December 07, 2011 10:48 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] lab chairs

Where do  you recommend buying lab chairs?  I'm looking for some w/o arm rests, 
solid black or tan, on rollers, etc..


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[Histonet] RE: cassette marker

[Goins, Tresa]

Laboratory Marking Pens from Thermo Scientific (Richard-Allan) Ref 2000.  Best 
for staying on but sensitive to writing on surfaces that are not absolutely dry.









-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Saturday, December 03, 2011 11:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cassette marker



Can anyone recommend a marker for using on cassettes? We currently use pencil, 
which sometimes smudges. We've tried a few markers already, but some fade, 
while others hold up well for processing, but won't when placed in decalcifier.

Vendors are welcome to respond.

Thanks,

Toni









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[Histonet] RE: slide labeling

[Goins, Tresa]

Hi Lisa -

We hand write our labels, but do so prior to cutting sections.
  
The accession numbers [unique to every slide] are read from a printed data 
sheet and written on the slides.  This allows verification that the numbers 
match [slide and block] as the tissues are cut.  If the numbers are written at 
microtomy I see it as a source of possible labeling errors.

All blocks and slides are processed in numerical order so there is a number 
check at embedding, at cutting and at final labeling.


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax

   

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa
Sent: Monday, October 31, 2011 10:09 AM
To: 'histonet@lists.utsouthwestern.edu'; 
'histonet-requ...@lists.utsouthwestern.edu'; 
'histonet-boun...@lists.utsouthwestern.edu'
Subject: [Histonet] slide labeling


Hi,
Just curious because this questions has come up multiple times from 
non-histology people so I thought I'd post it here. When labeling slides at 
microtomy what info do you include on the slide? I know the automated slide 
labelers that are out there will include patient name, surgical number etc.; 
but when handwriting slides as you cut what do you write on them? Currently we 
write the case number, part number and level as well as the cutters initials. 
After staining we put the permanent label on that has patient name and case 
number etc (hence two identifiers). Is there anyone out there who hand writes 
the patient name on the slide or a second identifier on it at the time of 
microtomy?
Thanks for your help,
Lisa

Lisa V.
Chg., IL

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RE: [Histonet] How to save dried antibody?

[Goins, Tresa]

If the antibody has precipitated, it may be impossible to restore activity.  
The glycerol is present to keep the suspension in liquid form - and it sounds 
like you no longer have a liquid.  Try adding an appropriate suspension buffer 
and keep your fingers crossed.  If you do not have previous experience with 
this antibody and know what to expect regarding performance, I would discard it 
and re-order.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah Tabatabaei
Sent: Friday, October 28, 2011 9:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] How to save dried antibody?



Dear All,

We have purchased a sheep anti-CGRP antibody for our fluorescent IHC study on 
human tissue and kept it in -20 for a while. It came in liquid but now it's all 
stiff 
and looks like it's being crystallized. I cannot pipet any of it. It 
has hardened and looks like a piece of glass at bottom of its container. I 
tried to re-suspend it with the same amount of glycerol, but the 
antibody doesn't seem to be mixing with it.

Does anyone know how to save this antibody? How can I bring it back to its 
liquid state? 

Thank you for your time

 
Sarah Sadat, DDS


MSc Student, Dental Sciences
McGill University
Montreal QC.

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[Histonet] RE: DRYING TIMES for immuno stains

[Goins, Tresa]

Air dry over air vent for a minimum of 30 min
Into 60C oven for 15 min
Direct transfer to hot water-detergent bath for de-wax


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Thursday, October 20, 2011 6:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DRYING TIMES for immuno stains

Can you tell me how long you let your IHC slides air dry at room
temperature  and how long you put them in the oven prior to staining?

 

Thanks

Diana

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RE: [Histonet] Rocky Mt Spotted Fever

[Goins, Tresa]

You might try contacting Rocky Mountain Lab in Hamilton Montana 
http://www.niaid.nih.gov/about/organization/dir/rml/Pages/default.aspx 


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Thursday, September 29, 2011 4:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rocky Mt Spotted Fever

I know that I have requested this information once before but am in need of
it again.  Does anyone know of a company that tests for Rocky Mt Spotted
Fever?  We are having a  hard time finding someone that still carries an
antibody that tests for this.

 

Any and all info greatly appreciated.

 

Sheila

KDL Pathology

Knoxville, TN

 

 

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[Histonet] RE: Bovine corona virus antibody

[Goins, Tresa]

BCV clone BC-8F2-C from RTI - retrieve with trypsin digestion for 15 min - our 
titer is 1:250 but we have used the same lot for more than five years.


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sverlow, Karen
Sent: Friday, September 09, 2011 1:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bovine corona virus antibody

I am looking for antibody recommendations for our bovine corona virus IHC. The 
clone we used previously is no longer available.

Thanks,
Karen Sverlow, HTL (ASCP)CM
Histology Supervisor
California Animal Health and Food Safety Laboratory System
Thurman Building, West Health Sciences Drive
Davis, CA 95616
530-752-8753
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[Histonet] RE: MSDS

[Goins, Tresa]

The hard copy of the MSDS is for your own safety AND for the safety of any 
person responding to an emergency.

If you work with the reagent, you should be familiar with the associated 
hazards.  Someone from down the hall or emergency response personnel will not 
necessarily be familiar with the associated hazards.

I don't know about you, but I sometimes have trouble locating files on my own 
computer - I would hate to depend on someone else trying to find a MSDS file on 
a computer prior to administering aid.

The skill of access by alphabetical means is something everyone is familiar 
with - a hard copy of the MSDS is a necessity.

My two cents,

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Friday, August 26, 2011 9:29 AM
To: Cheryl; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Jill's MSDS question: a tech's perspective

I would respond to this by saying, if I were working with something that
dangerous, I would know ahead of time what to do with it.  I'm not going to
spend any amount of time looking up an MSDS if something is on me RIGHT NOW,
digital or not.  Which is why I think it makes sense to have digital copies,
because you're going to know how to handle hazardous stuff and digital
copies save space and time.

Emily


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Fri, Aug 26, 2011 at 10:17 AM, Cheryl tkngfl...@yahoo.com wrote:

 Hi Jill-- Congrats on the job!

 The MSDS in paper vs. eCopy copy will come down to the opinion of your
 inspector.  Like Wil says, he's passed before...and I've passed before but
 only because we had both eCopies and hard copies.  Most of the larger
 for-profit and major health system administrations require both by SOP
 regardless, for liability reasons usually but please read on!

 Better yet, think of the practical uses and real-world applications
 of this rather than the 'do it because I have to' ones:

 If you or one of yours gets splashed with something--how do you want the
 next response to go?  If you only have eCopies and the power is down, or
 you are having computer issues, or the PC is slow, or the printer on the
 network isn't responding, then what? If it's your eyes or your skin that's
 about to be irreversably burnt, wouldn't you want that information available
 in a TOTALLY reliable manner and a copy available to grab, follow AND send
 to the emergency room with you--RIGHT NOW?  Then add to it the liability for
 your company should this be the case...and we all know when you most need
 your PC to work is when it doesn't.

 If I'm having trouble making a decision often exaggerating the potential
 circumstances makes the answer far easier to come to.

 If I am working in a lab I want hard copies alphabetized by both the mfc.
 chemical name and the common name we use for it in the lab with an
 index with the binders (Print the covers black on yellow paper and insert
 these in clear view binders) on the benches in the space where we work.  Not
 on a computer or off in the transcription area.  It may not be required by
 your inspector or by your facility, but is that really why we have these in
 our labs in the first place--because we're required to do so?

 My four cents

 Cheryl

 Cheryl Kerry, HT(ASCP)
 Full Staff Inc - Staffing the AP Lab one GREAT tech at a time.
 281.852.9457
 ad...@fullstaff.org
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[Histonet] RE: Old Green Staining Racks

[Goins, Tresa]

Mercedes Medical MER1040 - Slide Staining Dipper, with metal handle - took me 
awhile to find them too.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura
Sent: Tuesday, August 09, 2011 9:39 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Old Green Staining Racks

Does anyone remember the old green staining racks that had an inverted L 
metal handle and held 25 slides?  We are trying to find somewhere to order 
them, but having no luck.  Any suggestions are welcome!  And, thanks in 
advance!  The Histochicks at Sharon Regional



Sharon Regional Health System is the area's largest hospital
and provider of health care services. Visit us online at
http://www.sharonregional.com for a complete listing of our
services, primary care physicians and specialists, and satellite locations.

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RE: [Histonet] your opinion on microtome

[Goins, Tresa]

We have a Reichert-Jung 820 manual (old old old) and a fully automated Microm 
HM 355.  Both of us who cut prefer the manual.

The manual is more compact, easier to clean, cheaper. 
The Microm HM 355 is twice as big and the blade holder - adjustment screw 
set-up is neither convenient or easy to optimize.

Bottom line, the fewer moving parts the better.

Tresa 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
amitapan...@torrentpharma.com
Sent: Friday, August 05, 2011 1:25 AM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] your opinion on microtome

Hello friends,

I am planning to  buy a microtome for my lab i already have Leica 
manual one which is working perfectly.

Now I wish to introduce a fully motorized one. 

Short listed items are: Leica RM2255, Microm 355S, Shandon Finess ME.

Let me know your frank review on
1. manual versus automated option
2. Above all three items.in terms of their performance, maintenance and 
breakdown etc. or due you suggest any more better option


Thanks in advance for your feed back. In past also histonetters helped me 
to solve many problems, looking forward this time also.

Amita
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RE: [Histonet] Antibody suggestions for dog and cat

[Goins, Tresa]

Hi Mark -

We have had good results with: 
Vimentin Clone V9 (CM048), Factor VIII (PAb-CP039) and pan-Cytokeratin, 
Clone Lu-5 (CM043) from Biocare
CD3 C7930 from Sigma
CD79a Clone HM47/A9 (MS357), Melan A (MS799) from Thermo Scientific - 
no luck with CD20.
CD117 (RB9038) from Thermo Scientific - data sheet states heat 
retrieval is required, but we get good results without retrieval.

I have also found that while Melan A is excellent in canine tissue, S100 
(PA1-38584, ThermoSci) works better in feline tissue - more positive hits with 
higher intensity of specific staining. 

Best regards,


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Monday, July 25, 2011 12:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody suggestions for dog and cat

Could anyone suggest suituble antibodies for the following markers in dog
and cat tissue:

Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII

Also could use a suggestion for C-kit in Dog tissue only.

thanks

Mark
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RE: [Histonet] Antigen retrieval survey

[Goins, Tresa]

I hate to be pessimistic, but different clones of monoclonal antibodies can 
have different optimum retrieval requirements, not to mention tissue type, 
source species, etc.  

What I do believe however, is that enzymatic retrieval is under-sold - no 
equipment purchase is necessary so you will not see companies advocating their 
use.  Nearly half of the antibodies we use are optimally retrieved with enzymes 
(pronase, trypsin, pepsin) rather than heat retrieval.  


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper
Sent: Wednesday, July 13, 2011 9:10 PM
To: Histonet
Subject: [Histonet] Antigen retrieval survey


Hi All,
 
I am doing a survey and will be happy to compile results and share if folks 
will respond! What is your favorite antigen retrieval method and/or panel?
 


Buffer

Source/composition

Temperature

Device
 
Thanks, Andrea
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[Histonet] RE: Counter top immunos

[Goins, Tresa]

We also do our immunos manually - but we use no kits.  I do not promote but do 
suggest:
 - Biocare Vulcan Fast Red as chromogen - air dry slide after counterstaining 
with hematoxylin, then coverslip
 - KPL for linkers
 - concentrated antibodies; ready-to-use are good to check to see if the assay 
works, but shelf-life is longer if concentrated

Good luck and have fun!


Tresa Goins
Histopathology Supervisor
Department of Livestock
Bozeman, Montana

   

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, 
Kimberly K
Sent: Thursday, June 02, 2011 7:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Counter top immunos

Hello Histo folks
 
  Hope everyone is having a great weekI am writing to get some info
on immuno kits for staining just by hand, like back when I first started
in this field.  We are a small lab in Alaska and all of our immunos are
sent out of state,  We are looking to do some of them here and try to
save money, but don't really need a stainer.   So are there any kits
that are better than others?  Is there anyone that does immunos this way
that would give some much needed advise???
 
  Again thanks in advance for all the imput...
 
Kimberly
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[Histonet] IHC Control Tissues

[Goins, Tresa]

If you haven't made up your mind yet on this issue, here are a couple of 
references that may  -  or may not  -  help:

Veterinary Diagnostics:

http://www.ncbi.nlm.nih.gov/sites/entrez?orig_db=PubMeddb=pubmedcmd=Searchterm=%22Journal%20of%20veterinary%20diagnostic%20investigation%20%3A%20official%20publication%20of%20the%20American%20Association%20of%20Veterinary%20Laboratory%20Diagnosticians%2C%20Inc%22%5BJour%5D%20AND%2020%5Bvolume%5D%20AND%20393%5Bpage%5D%20AND%202008%5Bpdat%5D

FDA

F.20 Quality Control:


http://www.fda.gov/downloads/MedicalDevices/DeviceRegulationandGuidance/GuidanceDocuments/ucm094015.pdf


Tresa Goins
Histopathology Supervisor
Department of Livestock
Bozeman, Montana

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[Histonet] RE: CD79 alpha dilemma

[Goins, Tresa]

We use Cd79a clone Ab-1 (HM47/A9) from Thermos Scientific (Cat #MS-357).  It 
does not list either cats or dogs on the data sheet but it works.  They also 
suggest HIER-Citrate pH6 for retrieval, but in our hands Citrate pH 9 produces 
better results - steam heat for 20 min and cool on bench top for 30 min - we an 
a non-automated lab.

Good luck,


Tresa Goins
Histopathology Supervisor
Department of Livestock
Bozeman, Montana



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Farish, Craig
Sent: Thursday, May 12, 2011 7:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD79 alpha dilemma

Hi folks - has anyone out there found a Cd79α clone and supplier which will 
cover multiple species reliably?
I can find clones which will stain cats and dogs (and humans), and clones which 
will cover horses, pigs, cows and primates (and humans, but not opossums for 
some reason??) but i'm yet to find one which will cross-react with both the 
large and small animals commonly encountered in a vet lab. I would rather not 
have to stock 2 versions of the same antibody if I can avoid it.
Any suggestions would be much appreciated.
As always, thanks to everyone who contributes to histonet,
Craig

Craig Farish
Senior Technical Officer
Veterinary Diagnostic Laboratory
School of Animal and Veterinary Sciences
Charles Sturt University
Boorooma Street
Wagga Wagga
NSW 2678
Australia
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RE: [Histonet] nuclear bubbling

[Goins, Tresa]

The best tip I ever received was from a gentleman in Australia who suggested 
adding a very small - so small it would appear to be useless - amount of Tween 
20 to the floatation water bath.  The entrapment of water is eliminated - no 
more blebs of water at the base of the sections.  Works like magic.


Tresa Goins
Histopathology Supervisor
Department of Livestock
Bozeman, Montana

  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela
Sent: Thursday, May 12, 2011 2:25 PM
To: Rene J Buesa; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] nuclear bubbling

Another individual called me and commented further, which clarified your 
statement.  It is actually poor lab practice to combine the drying/baking.  
These steps should really be done separately so as to not trap the water 
under the tissue.  I have noticed bubbles of water on the bottom of my slides 
that are encapsulated in wax.  I assume this is also happening under the tissue 
as well, therefore affecting the nuclear morphology.  I had been taught to 
speed up the process by placing them in the oven directly after cutting.  
Decreasing the TAT was all they really cared about.  Fortunately where I work 
now chooses quality over quantity and I can adjust my protocol to get rid of 
the artifact.  Thank you all for your comments and clarity.


Thanks,
 
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  nacaela.john...@usoncology.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela
Sent: Thursday, May 12, 2011 2:50 PM
To: Rene J Buesa; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] nuclear bubbling

So there really isn't a point in using an oven to dry the slides?  I currently 
dry my slides for an hour at 55-60 degrees C. 


Thanks,
 
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  nacaela.john...@usoncology.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, May 12, 2011 2:41 PM
To: Johnson, Nacaela; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] nuclear bubbling

Nacaela: 
Heat by itself is not the cause, but the heat applied to sections that have not 
been properly drained and still have water left between them and the slide, is.
You have to drain the sections properly before heating them.
Using formalin is not the cause.
René J.

From: Johnson, Nacaela nacaela.john...@usoncology.com
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 12, 2011 12:51 PM
Subject: [Histonet] nuclear bubbling

Has anyone one had problems with nuclear bubbling?  I have read about two 
different causes.  (1) Formalin itself is known to cause the issue and (2) 
heating the tissue at a high temp (70 degrees C or above) while drying.  The 
latter is definitely not happening, but I do use formalin.
I am in the process of changing the type of formalin that is used during 
collection.  Does anyone have any other suggestions?  Has this problem occurred 
for any other reason than I have already pointed out?  It seems to be worse in 
the surgical needle biopsies than in the bone marrow biopsies.


Thanks,



Nacaela Johnson, B.S. HTL (ASCP)CM

Histotechnologist

KCCC Pathology

12000 110th St., Ste. 400

Overland Park, KS 66210

Office:  913-234-0576

Fax:  913-433-7639

Email:  nacaela.john...@usoncology.com


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this message. If received in error, please immediately inform the sender and 
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[Histonet] RE: Immuno's

[Goins, Tresa]

Valerie -

All of our IHC stains are done manually.  
As an animal tissue lab, our antibody sources will differ, but the best 
chromagen I have use by far is Biocare's Vulcan Fast Red.  I have tried other 
sources (cheaper) for the new fuchsin chromagen, but they do not produce the 
same vivid red stain as Vulcan.

Our secondary antibodies are from KPL (biotinylated anti-mouse or anti-rabbit) 
with KPL alkaline phosphatase.

Good luck


Tresa Goins
Histopathology Supervisor
Department of Livestock
Bozeman, Montana



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
Sent: Monday, April 11, 2011 8:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Immuno's

Hi Folks..
 
Looking for a bit of help. Is there anyone out there who are doing their
immuno's manually?  Our Pathologist has just informed us that our
immuno's are ALL too weak.
He states that they always have been, as he compares them to slides that
we get back from the  reference labs that we use for the antibodies that
we don't do in-house.
 
If you are doing them manually...what companies are you using for
antibodies, detection kit and chromogen? And are your stains really
dark?
 
I informed our Pathologist that I know for a fact that the reference
labs that we use, do the immuno's on stainers, and naturally these
stains will be consistently be stronger.
 
I am trying to see if I can keep costs down by trying not to have to buy
a stainer and continue to do the immuno's manually.
 
If you all know of a wet-workshop or of a immuno. company who would send
someone to our facility to teach us ( the latter is preferred, since my
section chief is going to retire in 1 1/2 weeks, I will be taking over
her duties and this is going to leave us with only myself and one other
tech, until we can get a replacement for my position).
 
Any help or advice that can be given will be greatly appreciated.
 
Thanks so much.
 
Valerie Hannen, MLT(ASCP),HTL,SU (FL)
Parrish Medical Center
Titusville,Florida


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[Histonet] RE: Stupid Rabbit Primary Antibodies

[Goins, Tresa]

You might try Background Sniper from Biocare or Background Buster from Innovex 
- we process animal tissues exclusively and we use several rabbit primary 
antibodies.


Tresa Goins, Ph.D.
Histopathology Supervisor
Department of Livestock
Bozeman, Montana



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sgoe...@mirnarx.com
Sent: Tuesday, March 22, 2011 3:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!

So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal.  Does
anyone have any suggestions for how to eliminate the background?  I have
diluted almost to the point of the antigens not showing!  Thanks guys
and gals!!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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RE: [Histonet] paraffin for pathology lab.

[Goins, Tresa]

It sounds like you are looking for paraffin for infiltration (not embedding).  
If this is so, use a paraffin with shorter polymers like Richard-Allan Paraffin 
Type 1 - The same company makes Types 3, 6, and 9 but these longer polymer 
paraffins are designed for sectioning and ribbon formation.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of canan atacan
Sent: Thursday, March 17, 2011 10:33 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] paraffin for pathology lab.


 
Hi all 
 
I have a question. If you spent time and answer this I will be appreciated. 
 
To use in our pathology laboratory we need to get beaded paraffin. I need to 
order it from companies. 
 
According to my research I understand that the oil content, penetration depth 
... important for this product and I dont know what should % of this oil 
content be?
 
It will effect penetration dept? We need to get 56-58 C melting point parffin. 
 
Please inform 
 
thanks alot
 
Canan 
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RE: [Histonet] High Complexity Testing

[Goins, Tresa]

I must disagree with this assessment of what makes a test complex.  If the test 
is done properly [the responsibility of the technologist] then the reading to 
the test is a visual determination that requires experience on the part of the 
pathologist, but if the test is not done properly, will the pathologist be able 
to tell the technologist what to do to fix the problem? 

Where's the Tylenol?


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Tuesday, February 08, 2011 9:58 AM
To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner
Subject: RE: [Histonet] High Complexity Testing

While the test is high complexity it is the READING of the test by the 
pathologist that determines its complexity.  Because histotechs do not report 
the results our part of this test is not high complexity.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR   72202

phone   501.364.4240
fax501.364.3155

visit us on the web at:www.archildrens.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, February 08, 2011 10:32 AM
To: histonet@lists.utsouthwestern.edu; Sheila Fonner
Subject: Re: [Histonet] High Complexity Testing

When a machine is doing the test, there are stringent provisions as to the 
preparation and validations of the test.
Done manually, it requires a trained technologists and, yes, they are high 
complexity tests (both IHC and FISH, and their variations).
René J.

--- On Tue, 2/8/11, Sheila Fonner sfon...@labpath.com wrote:


From: Sheila Fonner sfon...@labpath.com
Subject: [Histonet] High Complexity Testing
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, February 8, 2011, 7:45 AM


Hello All,



I would really appreciate it if anyone has information on whether IHC/ISH
are considered high complexity testing for histotechs.  Our pathologist
believes that ALL histology low complexity testing since a machine is
doing the work.  Can anyone help me out with some guidelines, literature,
etc. that says otherwise?  I would really appreciate it.  We just want to
know which one it is.



Thanks so much Histoland!







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and protected from disclosure. If the reader of this message is not the 
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dissemination, distribution or copying of this communication is strictly 
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[Histonet] RE: Animal tissue processing

[Goins, Tresa]

The NSH has an Animal Processing Manual available at their WEB site. 


Tresa Goins
Veterinary Diagnostic Lab
Department of Livestock
Bozeman, Montana

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gill, Caula A.
Sent: Thursday, January 20, 2011 9:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Animal tissue processing

Hi All,
I work in a hospital where we process human tissue. As a favor to a
friend the pathologist would like us to process animal tissue. My
questions are could we process the animal tissue on the same processor
with the human tissue? And Are there different processing times and
reagents for animal tissue? Thanks for any help you can give

Caula (HT)
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RE: [Histonet] Xylene Substitute

[Goins, Tresa]

We use Clear-Rite 3 (Richard Allan Scientific 6901) from Fisher Scientific in 
our VIP processor and for staining.  I am not familiar with Permount, but we 
use Richard-Allan Mounting Medium (4111; also available from Fisher Scientific) 
that is toluene based - works great in combination with Clear-Rite 3.


Tresa Goins
Veterinary Diagnostic Lab
Department of Livestock
Bozeman, Montana

  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Monday, October 25, 2010 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute

Hi all!
I'm looking for recommendations for a xylene substitute for our processing and 
staining. We have two VIP's and a Leica stainer. We use Permount for 
coverslipping (manually with glass). Does anyone use a xylene substitute 
that you would recommend for this combination?
Thank you!

 
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.


  
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RE: [Histonet] mislabeling of slides and blocks

[Goins, Tresa]

If mistakes occur, the lab protocols must be altered to prevent the mistakes. 

 If personnel can not or will not follow protocol to eliminate mistakes, then 
employment must be terminated.

This is not being picky or unreasonable - ask any potential cancer patient 
relying on an accurate diagnosis.  


Tresa Goins
Bozeman, Montana


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of sgoe...@xbiotech.com 
[sgoe...@xbiotech.com]
Sent: Friday, October 08, 2010 1:30 PM
To: Amy Farnan
Cc: histonet@lists.utsouthwestern.edu; Sherry Dean
Subject: RE: [Histonet] mislabeling of slides and blocks

Currrently I am working in a lab where I am the only tech., but in
previous labs when mislabeling happened, the tech would be written up
for it after one formal verbal warning.  If they were written up 3 times
in 6 months, then a suspension was possible (although never happens),
then if the problem continued could lead to being fired.  So, basically
you would have to mislabel something or another 6 times in one year to
be fired.
Again...just my opinion...


Sarah Goebel, B.A., HT (ASCP)

Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-5107




 Original Message 
Subject: RE: [Histonet] mislabeling of slides and blocks
From: Amy Farnan farn...@nehealth.com
Date: Fri, October 08, 2010 10:52 am
To: Janice A Mahoney janice.maho...@alegent.org,
sgoe...@xbiotech.com
Cc: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu, Sherry Dean
sherry.d...@ventana.roche.com

 Sarah,

What is your policy?


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