[Histonet] (no subject)
Hi colleagues We are going for Digital Pathology What is your recommendation What's the best device and company (Aperio, Philips, 3DHISTECH) Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent from my cell phone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOP share
Hi colleagues I hope all are fine Please share me the SOP for: 1.Leica ASP6025 Automatic Tissue Processor. 2.Tissue-Tek Prisma & Film Automated Slide Stainer & Film Coverslipper. 3.Leica IP C Inkjet Printer for Tissue Cassettes. Best Regards, Jamal M. Al Rowaihi Al Borg Medical Laboratories I Anatomic Pathology Supervisor I Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 503629832 <http://www.alborglaboratories.com/> www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] unsatisfactory Thin prep
Dear colleagues I appreciate if some can share me their statistics in unsatisfactory ThinPrep and what it's the best solution to reduce the amount of unsatisfactory results. Best Regards, Jamal M. Al Rowaihi Al Borg Medical Laboratories I Anatomic Pathology Supervisor I Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 503629832 <http://www.alborglaboratories.com/> www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Marking Tissues with Eosin
Hi If you are using buffered Formaline so the Eosin color will not resist until the end of tissue processing.I recommend to add small amount of stock Eosin to the last alcohol in theTissue processor. Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent from my cell phone Original message From: Mca Werdler via Histonet <histonet@lists.utsouthwestern.edu> Date: 6/23/16 8:49 PM (GMT+03:00) To: "Rebecca E. Ashley" <p...@uwyo.edu> Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Marking Tissues with Eosin Dear Rebecca, Yes this is possible. just don't use a too strong concentration. The eosin should give a slight pink color on the tissue after processing and after embedding. Good luck, Maarten 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet < histonet@lists.utsouthwestern.edu>: > I had a biopsy today that was nearly impossible to see on the sponges > during embedding or in the block. I've heard mention of marking these with > eosin to make them easier to see. Has anyone done this? Or do you use > some other type of marking dye for this purpose? > Thanks for your input! > Rebecca > > Rebecca Ashley > Histotechnologist > Wyoming State Vet Lab > 1174 Snowy Range Rd. > Laramie, WY 82070 > Phone: 307-766-9946 > Fax: 307-721-2051 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ANP.22970 Annual Result Comparison
Good day colleagues I need your help in collecting the data for the bellow requirements for CAP, is their good reference to collect the benchmark statistics for ER, PR & Her2neu ? ANP.22970 Annual Result Comparison For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The proportion is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The proportion is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER-negative in each of these settings. Investigation is warranted if the proportion of negative cases is significantly lower in any of these settings. Jamal M. Al Rowaihi BCs, HT, Anatomic Pathology Supervisor Al Borg Medical Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] automatic stainers and tissue processors
Go for Sakura products with no hesitate. Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent from my cell phone Original message From: Silvia Bonner via Histonet <histonet@lists.utsouthwestern.edu> Date: 2/29/2016 11:09 PM (GMT+03:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automatic stainers and tissue processors Hello, We are looking into purchasing new automatic H strainers and new tissue processors. Any helpful advice? I know this may have been discussed before but I an new to histonet. Thanks for your help! Silvia Bonner, HT(ASCP) CM Histology Supervisor sbon...@pathregional.com<mailto:sbon...@pathregional.com> Pathologists' Regional Laboratory 1225 Highland Ave Clarkston, WA 99403 This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (509) 758-5576 and delete all copies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Lead Tech/ Supervisor Competency
Thanks FawanI think some are not completely satisfied with their papers that's why they didn't respond to us, but I think we suppose to share our experience to improve the quality, soon I will send you my own and I hope you send me yours... Good luck. Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent from my cell phone Original message From: Fawn Bomar via Histonet <histonet@lists.utsouthwestern.edu> Date: 2/19/2016 5:36 PM (GMT+03:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lead Tech/ Supervisor Competency Hi all, I didn't receive any responses in regards to my last post so I thought I would try one more time. Does anyone have a competency or orientation checklist that they use for lead techs or supervisors that they would be willing to share? On my last post, everyone that messaged asked for copies of my assessment and the assessments that were sent to me, which I did send to everyone that requested them. If anyone else would like me to send my assessment to the them to look at, please let me know and I will be happy to share, plus I would be appreciative of any feedback- positive or negative- and any suggestions for improvement. I tried to send an attachment of my assessment to the histonet but it kept saying it was waiting for moderator approval and it never was sent. Thank you all for your help and guidance. Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nuclear Bubbling
Great, I agree Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent from my cell phone Original message From: "Manfre, Philip via Histonet" <histonet@lists.utsouthwestern.edu> Date: 2/16/2016 10:44 PM (GMT+03:00) To: Rene J Buesa <rjbu...@yahoo.com>, "Vickroy, James" <jvick...@springfieldclinic.com> Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Sort of a rude response to someone looking for help. -Original Message- From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain → "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.rené On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" <histonet@lists.utsouthwestern.edu> wrote: Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/
Re: [Histonet] PAP stain quality
You need to review the slides fixation and what chemicals you are using Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent from my cell phone Original message From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> Date: 2/4/2016 5:50 PM (GMT+03:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAP stain quality Not sure if anyone out the would know the answer to this. We are having an issue with our PAP stained slides appearing too orange and look aged. If you have any idea for causes I appreciate any help -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] COMPETENCY ASSESSMENT for HISTOTECHNOLOGIST & CYTOTECHNOLOGIST
Dear Colleagues I will be happy if someone share me the current CAP accepted form for: COMPETENCY ASSESSMENT OF HISTOTECHNOLOGIST & CYTOTECHNOLOGIST Best Regards, Jamal M. Al Rowaihi Al Borg Medical Laboratories I Anatomic Pathology Supervisor I Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 503629832 <http://www.alborglaboratories.com/> www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Diapath Histology Machines
Hi Histonet Anyone have experience about the Diapath Histology Machines . Automatic Tissue Processor For Histology. Specimen., DONATELLO, open system 52PRO03 . <http://www.diapath.com/dettaglio.aspx?id=SDSGS9000> Semi-automatic microtome Galileo SEMI - Diapath . CANOVA EMBEDDING MACHINE. MODULE (110-230V) 01pc. . CANOVA COLD PLATES MODULE (230 V) 01 pc. . Flotation bath DPH 35. Best Regards, Jamal Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Removing paraffin sections from glass slides
Dear Colleague I just want to remind you.. After all of that procedure of removing the paraffin sections from the positive charged slides. The slides will not be positive charged anymore and not suitable for IHC. Also the cost of the detergents and the chemicals which you used for removing the tissue section is more costly than the slides. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: Weems, Joyce K. [mailto:joyce.we...@emoryhealthcare.org] Sent: Monday, June 22, 2015 5:53 PM To: 'Coffey, Anna (NIH/NCI) [C]'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Removing paraffin sections from glass slides This is what I would do... Soak the coverslip off in xylene Either - rehydrate back to water and just wipe the tissue off - then dip the slides in absolute and let dry.. OR - air dry out of xylene and wipe off with a wet gauze - then do the alcohol dip Whichever worked best. Just my 2 cents.. Happy Monday!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.cof...@nih.gov] Sent: Monday, June 22, 2015 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing paraffin sections from glass slides Hello, I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides. Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.cof...@nih.gov 301-846-1730 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] competency assessment for Anatomic Pathologist
Dear colleagues I hope if any one share me the form of: competency assessment for Anatomic Pathologist Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://www.alborglaboratories.com/ www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fixation of frozen section
You can't use full concentrate alcohol on such specimen, otherwise you will destroy the cells membrane. For me I am using 80% Ethanol. Best Regards, Jamal Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manahil Sent: Tuesday, April 28, 2015 7:23 PM To: Keyser Gerald T Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fixation of frozen section What about the Absolute Methanol? Sent from my iPhone On Apr 28, 2015, at 8:16 PM, Keyser Gerald T gkey...@uwhealth.org wrote: Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin. But, it's effective and far less toxic than formalin. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manahil Sent: Tuesday, April 28, 2015 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation of frozen section Hi histonet, Would like to query about which fixative do you prefer for fresh frozen section slide before HE staining? Thanks for your advices Manahil HTL/ ASCP Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Validation Procedure
Hi Allison I hope the attached can help you Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, January 29, 2015 10:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation Procedure Hello to all in Histoland. Does anyone have a tissue processor validation procedure that they would be willing to share? Any help in this would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CONFIRM anti-Melanosome (HMB45) Mouse Monoclonal Primary Antibody, Catalog Number 790-4366
Dear colleagues I am not ok with staining of Melanosome (HMB45) Ventana using BenchMark GX The expiry due date still valid and I follow the staining protocol from the pamphlet but no good stain show even on the melanoma control sections. The protocol is : Recommended Staining Protocol for CONFIRM anti-Melanosome (HMB45) with ultraView Universal Red on BenchMark XT. CONFIRM anti-Melanosome (HMB45) Mouse Monoclonal Primary Antibody, Catalog Number 790-4366 Procedure Type Method BenchMark XT Deparaffinization Selected Cell Conditioning (Antigen Unmasking) None required Enzyme (Protease) None required Antibody (Primary) Approximately 8 minutes, 37 °C Counterstain Hematoxylin II, 4 minutes Post Counterstain Bluing Reagent, 4 minutes Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://www.alborglaboratories.com/ www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Non-GYN Prep Safety
We process all conventional cytology specimens inside safety cabinet even the centrifuge. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Reichard Sent: Wednesday, December 03, 2014 7:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Non-GYN Prep Safety Question for you all Do you process Non-GYN specimens under a hood? What type of precautions are taken for suspect mycobacterium specimens? Are your ThinPrep machines under a hood as well? Thanks in advance Amanda Reichard, HTL (ASCP)cm Histotechnologist Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek
Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Tuesday, November 25, 2014 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek
Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Tuesday, November 25, 2014 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek
Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Tuesday, November 25, 2014 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Specimen numbering systems
I am doing the same of Joelle Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver Sent: Friday, November 21, 2014 10:30 PM To: Willis, Donna G.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Specimen numbering systems Numeric for the Specimen and Alpha for the Block. Joelle Weaver MAOM, HTL (ASCP) QIHC From: donna.wil...@baylorhealth.edu To: histonet@lists.utsouthwestern.edu Date: Fri, 21 Nov 2014 19:02:56 + Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC general fixation
Good day I recommend to see below study http://biospecimens.cancer.gov/meeting/brnsymposium/docs/Grizzle.pdf Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William J. O'Connor III Sent: Wednesday, November 12, 2014 8:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC general fixation I'm looking for input on the latest theories of duration of fixation for routine IHC. In the past, it was thought that tissues should be removed from 10% NBF after a max of 72 hours, and if not immediately processed, transferred to 70% ethanol until processed to retain antigenicity. What is the current thinking/trends? Over the last couple of years, I have heard that leaving tissues in 70% ethanol may be doing more harm than good, and it's NOT a good idea to leave them in alcohol for extended periods of time. I would appreciate your input, or point me towards any new data. Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Validation Procedure
Dear colleague I hope the attached help you Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Thursday, November 06, 2014 11:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation Procedure Hi, I am wondering if you guys in histoland will like to share your Tissue Processor Validation Procedure with me. Thanks, Adesuyi == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue falling off positive slides
Do you use Autostainer? What brand ? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Thursday, November 06, 2014 6:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue falling off positive slides WE are having trouble with tissue coming off our he slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 judith_par...@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica ASP6025 Processor
Good day Paula Lucas I have Leica ASP6025 processor since about 6 months. I am processing about 200 to 250 biopsy cassettes on that machine and I recommend to have it, it's smart machine, very easy to use and reagent money savings. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, November 06, 2014 12:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica ASP6025 Processor Hello, We're considering the Leica ASP6025 processor for our laboratory and I was hoping I can get pros and cons or any comments such as in performance, reagent money savings, ease of use, any comment from you. Thank you so much in advance for taking the time to give me your thoughts, Paula Lucas Lab Manager Bio-Path Medical Group California ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Cap ER/PR
I agree with Tim Morken Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, October 29, 2014 8:15 PM To: 'Heckford, Karen - SMMC-SF'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cap ER/PR Karen, when this has happened in the past CAP has told me to run the test and score it, then when the survey summary comes with the scores and discussion, to have the pathologist review and score the answers and document the review. It's about the best you can do when something gets missed. You may want to have two reviewers sign off in this case. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cap ER/PR Good Morning, Apparently while I was out on disability a CAP ER/PR PM2-A 2014 did not get done. Somehow one of our pathologists got it and forgot she had it. That being said, does anyone have a equivalent test they do instead. We only do this CAP testing to show competency. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Proficiency Testing (PT) or Interlaboratory Education Comparisons or Education Program
Hi According to my understanding and practicing, the PT must cover all your test menu: Surgical Pathology, Cytopathology Immunohistochemistry. ANP.02000 Education Participation Phase I As applicable, the laboratory participates in a peer educational program in anatomic pathology (e.g. CAP Educational Anatomic Pathology Programs). NOTE: The laboratory should consider participation in programs appropriate to its scope of service. Such programs provide valuable educational opportunities for peer performance comparisons in both technical and diagnostic arenas. While none of these completely emulates the precise clinical setting involving anatomic pathology preparations and rendering of anatomic or clinical diagnoses, they can be a useful benchmark of peer-based performance in a national database. Evidence of Compliance: ✓ Records such as CAP order form, purchase order AND ✓ Completed/submitted results indicating that the laboratory is participating in a CAP educational AP program OR records of enrollment/participation in another AP peer educational program OR records for participation in a laboratory-developed program by circulating case material with other laboratories or within the laboratory's own practice with documentation of peer review CYP.00125 PT ParticipationPhase II For laboratories subject to US regulations that perform gynecologic cytopathology, the laboratory and all individuals who examine gynecologic preparations participate in the CAP Gynecologic Cytology PT Program (PAP PT) or another proficiency testing program in gynecologic cytopathology approved by CMS. NOTE: This checklist requirement applies only to US laboratories and other laboratories subject to CLIA regulations. Laboratories must maintain documentation of PT performance for at least 2 years. Documentation must be kept for each individual participating in annual PT, including identification of those who are retested; documentation of remedial training; records of imposition of limitations on slide examination; and records of re-examination of slides, as required by CLIA. Evidence of Compliance: ✓ Written policy describing handling of PT failures (may include retesting, remedial training, and imposition of limitations on slide examination) AND ✓ Records that the laboratory is enrolled and all currently employed personnel have successfully completed PT AND ✓ Records of retesting, remedial training and imposition of limitations, if applicable AND ✓ Records of notification to the PT provider and CMS for any PAP testing personnel who left employment prior to completion of annual PT REFERENCES 1) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Extension of certain effective dates for clinical laboratory requirements and personnel requirements for cytologists. Fed Register. 1994(Dec 6):62609 [42CFR493.855] Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ian bernard Sent: Sunday, October 26, 2014 6:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Proficiency Testing (PT) or Interlaboratory Educational Comparisons or Education Program Are these terms interchangeable one in the same for Anatomic Pathology or is PT just for the Clinical side of the house? We participate with the PIP for Surgical Pathology, ASCP Case Reports for Non-GYN Cytopathology and the HistoQip for two of its series. Inputs. IB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Histonet Digest, Vol 131, Issue 26
Hi I am still recommending VIP5 for you I have: Tissue Tek VIP5 Jr Leica AS 6025 And Leica TP1020 Both of Tissue Tek and Leica are very good but according to your little volume (less than 100 cassette daily), VIP5 Jr are your suitable machine. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy Hu Sent: Friday, October 24, 2014 5:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 131, Issue 26 *Regarding processors* *I also need to help on determine which processor for our core. Our majority tissue is bone. * *Right now, we like VIP serials because of vacuum/pressure plus agitation. Please help me* *on this VIP processor, I never used this kind one. We are very small volume* *core, also do frozen and plastic undecal bone. We have a Shandon Excelsior now and feel the * *vacuum volume for this 10 years old processor is not enough for bone. * *Any input for this will be greatly appreciated. * *Dorothy* *MGH endocrine histocore* 9. RE: Processors (Joelle Weaver) 11. Re: Processors (Jay Lundgren) Message: 9 Date: Thu, 23 Oct 2014 19:05:23 + From: Joelle Weaver joellewea...@hotmail.com Subject: RE: [Histonet] Processors To: Jamal j.rowa...@alborglaboratories.com, 'GMail' nguy0...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: snt149-w792d852db98a977b194067d8...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Agree Joelle Weaver MAOM, HTL (ASCP) QIHC From: j.rowa...@alborglaboratories.com To: nguy0...@gmail.com; histonet@lists.utsouthwestern.edu Date: Thu, 23 Oct 2014 11:25:23 +0300 Subject: RE: [Histonet] Processors CC: hi colleague if your daily processed specimens less than hundred, go ahead for VIP5. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of GMail Sent: Thursday, October 23, 2014 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processors Please help me decide on a processor, I am currently inquiring about refurbished processors for a small derm path lab with very low volumes. I have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k and a Leica TP1020 type 4 for $17. Which one would you recommend? What's durable and won't break down often? Should I go for the vip2000 compared to vip5 to save money? Sincerely? Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processors
hi colleague if your daily processed specimens less than hundred, go ahead for VIP5. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of GMail Sent: Thursday, October 23, 2014 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processors Please help me decide on a processor, I am currently inquiring about refurbished processors for a small derm path lab with very low volumes. I have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k and a Leica TP1020 type 4 for $17. Which one would you recommend? What's durable and won't break down often? Should I go for the vip2000 compared to vip5 to save money? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Slides from antibody validation
Hi Lynne Bell I also keep all my slides even blocks which was used for validation, the CAP inspectors sometimes request to see them. I agree with you Joe W. Walker I am also using the same Slides Holder Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, October 14, 2014 8:58 PM To: Bell, Lynne; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Slides from antibody validation On a recent CAP inspection, we did have an inspector ask to see our slides from our HER2 validation. For the past 5 years, we have kept all of the slides from our validation studies along with the paperwork in a notebook. These glass slide holders have worked well for keeping everything together in one place for inspections: http://www.rochester100.com/store/p/195-SL-3-182.aspx Interestingly enough, on a recent Joint Commission inspection last year, an inspector made a similar request to see an example of an IHC that we had validated. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewal...@rrmc.orgwww.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition® and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Tuesday, October 14, 2014 12:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Slides from antibody validation Following antibody validation studies, do you keep your slides accessible for CAP to peruse during inspection? Has anyone had CAP actually ask to see your slides? I feel that keeping the validation forms, signed off by the medical director, is sufficient, but I would like other opinions. As always, thanks for any and all guidance! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Negative Control for Validation
Hi Linda can you give us more details about the controls selection and processing procedures. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Tuesday, September 30, 2014 12:43 AM To: 'Arrington, Karla A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Negative Control for Validation Hey Karla, We use Vimentin as a positive reagent control, our negative control serum for the negative reagent control and for the negative tissue control, the pathologist reviews the antibody stained slide and OK's the internal negative tissue elements as appropriately negative. Hope that helps, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A Sent: Monday, September 29, 2014 1:25 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Negative Control for Validation We are beginning the process of validating new antibodies using the BenchMark XT but have some questions for validation. Question: For a positive reagent control that stains appropriately positive, is a known control block used? If not, what is used? Question: For a negative reagent control that stains appropriately negative, what is ran? Question: Is a negative tissue slide ran for all of our 10 cases per antibody, or per case that will be used for validation? Thanks All! Karla Karla Arrington, HT(ASCP), HIT(AHIMA) Lead Histology Technician ANMC Pathology 907-729-1810 kaarring...@anthc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Validation:
Hi Jb The attached is my validation form you can refer to it. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Friday, September 05, 2014 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation: Does anyone have a tissue processor validation form that they are willing to share? Thank you. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] No food or drink in the lab
In our laboratory this matter are controlled by the safety department which give safety orientation for every new staff which include instruction about handling the food and drinks in the working areas and the disciplinary action which will be done in case of occurs by staff, and the staff will sign acknowledge on that orientation. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, September 04, 2014 4:24 PM Cc: Histonet Subject: [Histonet] No food or drink in the lab Does anyone know of a specific site and quote that states No food or drink in the lab and what the consequences would be? That covers drinks/snacks in the cabinets above their workstations too, right? I have a sign up on the door entering the lab that states no food or drink, but the staff continue to keep stuff around their desk, so I need something to back me up from CLIA, OSHA, CAP, etc... Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslipper
Dear colleagues Do any one have experience or information about Leica Coverslipper ST5010-CV5030 Workstation stainer. I am planning to add a new autostainer with coverslipper but I am confused between this brand and Tissue-Tek R Prisma R Coverslipper. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://www.alborglaboratories.com/ www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cytospin validation
Good day colleagues Ignoring my previous message what it means: no one did validation for Cytospin !! or no one want to share his information ?? or no one received my message !!?? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://www.alborglaboratories.com/ www.alborglaboratories.com From: Jamal [mailto:j.rowa...@alborglaboratories.com] Sent: Monday, August 11, 2014 1:47 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Cytospin validation Hi I need to do validation for my Shandon Cytospin 4, can anyone send me the validation procedure and form. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://www.alborglaboratories.com/ www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water/Bubbles
Make sure of the purity of the Abs. Alcohol in dehydration steps. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Julie Bowman Sent: Thursday, August 07, 2014 11:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water/Bubbles Hello all, Recently we have been experiencing a problem when running HE slides on our Gemini stainer (Thermo Shandon Varistain). At the end of the run, small water droplets or possibly air bubbles are seen on the slides after taking the slides out of xylene. If they are coverslipped, a white haze will be seen around the specimen after a few minutes. Any suggestions of what causes this and how to correct it? Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
Similar as me So strong, proud with high confidence Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vincent Rivera Sent: Thursday, August 07, 2014 11:58 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 15 Years and still going strong :) Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vriv...@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bubbles in mounting media
Hi Melissa Try to fill the tube of mounting media some hours before use. I use to fill it in the end of the day and in the next day the micro bubbles will be completely disappeared. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Melissa Ng Sent: Thursday, August 07, 2014 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubbles in mounting media Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770
I am also the same as Joelle Brian, It never been questioned by CAP inspectors but since it's in the common checklist we have to follow that’s why we have a policy with simple procedure and record, the procedure almost the same as Brian procedure but some time we use the digital PH meter. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver Sent: Tuesday, August 05, 2014 10:33 PM To: Cooper, Brian; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 Do the same as Brian. It is not that involved really, and its not worth getting a deficiency over. Joelle Weaver MAOM, HTL (ASCP) QIHC From: bcoo...@chla.usc.edu To: lcolb...@pathmdlabs.com; histonet@lists.utsouthwestern.edu Date: Tue, 5 Aug 2014 19:07:05 + CC: Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a yellow or purple column based upon what they see. If purple, they rewash, retest and document accordingly. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, August 05, 2014 11:06 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] whole slide scanners
Did you find out about Aperio ePathology, it’s the most famous technology for slide imaging You can visit the link below http://www.leicabiosystems.com/pathology-imaging/epathology/ Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 3:18 PM To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Histonet Digest, Vol 128, Issue 17
I totally agree with you in step 4, and I think the tissue was not fixed probably or not processed in good solutions, my suggestion for the time now problem is to stain the slides manual. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Saturday, July 19, 2014 6:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 17 Liz had some great suggestions! The one that I have found to be the MOST important is #4. Standing my slides up at a slight angle and allowing the moisture to drain out from under the section AND then letting the drained slides sit and dry for a good long time is extremely important for adherance to be successful (I've never gone as far as a week, although it couldn't hurt, but at least overnight seems necessary). I would focus on that, so you first get good adherance and then the other tips following the proper drying will then help keep them on. Good luck! -- Message: 4 Date: Fri, 18 Jul 2014 14:44:13 + From: Murphy, Valerie murp...@karmanos.org Subject: [Histonet] Brain tissue coming off slide To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: a0cd799e6fcd4949aad4a25377936712ae1da...@ex-mb2.kci-net.karmanos.org Content-Type: text/plain; charset=us-ascii We are staining human FFPE brain sections for Her 2 Neu and we're having a problem with tissue coming off the slide during the HIER step. We are currently using Vectabond coated plus slides and using a decloaking chamber for HIER. Are there any other steps we can take to prevent tissue coming off the slide? Thank you, Valerie Ratliff Karmanos Cancer Institute --- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. -- Message: 5 Date: Fri, 18 Jul 2014 09:16:04 -0600 From: Elizabeth Chlipala l...@premierlab.com Subject: [Histonet] RE: Brain tissue coming off slide To: Murphy, Valerie murp...@karmanos.org, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 14E2C6176416974295479C64A11CB9AE019C79ECD373@SBS2K8.premierlab.local Content-Type: text/plain; charset=us-ascii Valerie There are a few things that you can try. 1. This may or may not work with the Her2 IHC, since for that antibody I believe it requires retrieval at least at 98C but time and temperature are related with respects to heat retrieval. You could try 70C for 2 hours this is gentler on the tissue and works for some antibodies but not all of them. For example it does not work for Ki-67 in our hands. We use this retrieval method on bone and other types of friable tissue if necessary. 2. We have found that some samples after HIER may require manual staining methods in order to optimize tissue adherence, not sure if that is an option for you too. 3. Post fixation in 10% NBF after deparaffinization for about 10% minutes. This may help tissue adherence but we have also found that this may decrease the sensitivity of the protocol and you may have to adjust your primary antibody concentration. 4. Tissue adherence to the slide especially with brain (we have seen this on rodent brains) requires proper fixation and adequate processing, poorly fixed or processed brain samples will have issues with tissue adherence. 5. Allow the cut slides to sit for about a week prior to staining we find this may help with tissue adherence. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Validation of IHC
Dear colleagues We purchased Ventana GX from Rosh for IHC I reviewed the resent CAP check list 2013 for validation of IHC but I got confused. I am using ready to use prediluted antibodies from Ventana and I want to validate the antibodies before use according to CAP requirements. Can I have the validation plane and procedure for IHC antibodies. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://www.alborglaboratories.com/ www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
