what kind of controls, ie stains
TJUH
Sue
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We actually make them part of the gross description
TJUH
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I agree with William tape from anyone else but Sakura is not worth the time and
effort. We were just CAP inspected and they said nothing about tape. I have had
issues with tape and usually just cut around the sample on the tape and recover
on a slide, works beautifully. As far as the oven time
histo tech salaries are all over the place, hospital still do not recognize our
importance and it shows with the salary range. also with re-embursements being
cut it just runs down hill.
sue
tjuh
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I agree if the tissue is cut properly you should not need an extended program
but when you have residents and PA's they tend to rush and their sections are
thicker. We do not have an extended program still within the CAP limits and it
works quite nicely.
TJUH
Sue
cannot fix it. An old adage we are not magicians.
Sue Paturzo
TJUH
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we do not use freeze spray in the lab at all. this issue has really gotten me
bummed out since it is so sporadic. I am going to try to see if it is
associated to one day in particular. it is one PA but multiple techs have
identified the issue.
___
OMG we are experiencing the same issue. At first it was just GI and now we are
seeing it on prostate. One pathologist said it looks like the tissue has been
cooked. The only issue is we can have two biopsies right next to one another in
the basket one looks good and one looks bad. My director
they are being held in and
that temperature. If you are looking for a lean approach this is not it. If
they are already in a space I would ask what the space is going to be used for
and perhaps that use could be swiched.
TJUH
sue
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For the most part we release nothing to patients, all our releases are
approved by our Risk Management Department.. Hardware for litigation purposes
must be released to an approved storage lab. Anything that must be buried must
go through a funeral home.
Sue Paturzo
good luck, we no longer do the stain but we only were able to get it from
Sigma.
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I have seen these cassettes and while they are nice they are rather expensive
and with re-embursemnt down everyone is looking at their budget. Also due to
the design and the use of bar codes they do not always fit in the engravers. I
still like the idea but with the amount of prostates we do
We have a weekly schedule prepared monthly
Tech 1 rapids, special stains, IHC microtomy
Tech 2 bone marrow, cell blocks and assist tech 1, this tech can be pulled for
routines if possible
Tech 3 biopsies, HE stainer, and case distribution
Tech 4 our off site campus work, this tech also does
Home Depot or Lowes sells plastic scrappers in the paint area. I cannot see
why we would pay top dollar for this item. I also purchase brushed to clean
paraffin off microtomes at the craft store. 50 cents each is much better than
5 dollars.
Sue Paturzo
TJUH
We use Dragon with CoPath Plus and works great, you can make macros and I know
my PA have created some quick texts as well. We actually stared with Kurzweil
in the 80's and expanded to Dragon speak. the only time we do not use Dragon
is with Synoptic, although it works, it is labor intensive.
We use Ventana tracking system that is interfaced with our LIS and can track
all slides.
STP
TJUH
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like it very much, was not sure if I was going to like going back to labels but
it is not as bad as I though. Only issue is the cost but I am working with
other vendors to get cheaper labels.
STP
TJUH
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What we do here is run a mix of expected negative and positive cases this way
we are sure we are not getting exogenous staining. Depending on the antibody
we try to run at least a mix of 25 cases. In some cases the antibody proves
difficult to get a large amount of positive cases and we make
HP is difficult, we actually use a patien control from a positive case and cut
approx 20 slides from each block. You can probably purchase them but they are
costly.
Sue Paturzo
TJUH
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Have Ventana like it but cost is high, ave no experience with new DAKO but old
platform was nice
Sue Paturzo
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We had the same problems, tried oven did not work, the best plan is holding out
for at least 5 days prior to filing.
This is really not a good solution.
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I agree, if you do not document that a specimen was removed most likely
insurance will deny the clain.
SPaturzo
TJU
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forceps inside with ease. Another
reason I would like to replace the nylon bag is that when you get an aggregate
of tissue some of it gets inside the mesh and if there is minimal tissue I fear
you may miss some diagnostic material.
Sue PaturzoTJUH
hello
We collect one unstained for postate biopsies, 5 unstained for lungs (we id
each slide with a level number and do 5 unstained), if the biopsy is small the
histotech will take come unstained slides and hold them for about a week, we
actually place a dot on the block so the techs know
they have completed microtomy. The
tech that is in the front will cut biopsies, especially the ones for H-Pylori
and than go to the front for the remainder of the shift.
I know it sounds confusing but it actually works.
Sue Paturzo
TJUH
We actually just stared keeping a log for special stains, always for IHC. Our
rule of thumb is that an IHC control slide cannot be oler than 6 months,
although we go through most of them much faster. For the basic IHC, tonsil,
fallopian tube etc we do not log the case information for IHC
All you would need to do is id the tissue and dx on your paperwork. it should
not be any different than running each blocks alone.
susan T. paturzo
TJU
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Actually u would have too have 3 set ups since none can share anything.
Sent from my iPhone
On Jul 24, 2014, at 3:20 PM, Brandi brandihi...@hotmail.com wrote:
I am shopping for a new automated stainer and would prefer one that I can do
my HE stain as well as my PAP (GY AND NON-GY) stains
You can rub salt on it and it should come up, I our salt on counter and make a
thick scrub
You can use Iodine and than wash with Sodum Thiosulfate
Both work on hands the second one is a little more difficult to work with but
does work
Cape Regional Medical Center
Phone: 609-463-2170
Thanks,
Sue Breckenridge, BS, HT/HTL (ASCP)
Histology Supervisor
Cape Regional Medical Center
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Cape
Regional Health System contains information which
I used the Logs quick processor and it was really nice for small biopsies, no
experience with
their routine processor. Have 6 Excelsior's and love them.
STP
TJUH
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We do not allow formalin in the actualy OR, yet it is in the Ultrasound Suites.
There is a holding
area outside the OR with the formalin,
S Paturzo
TJUH
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We auto do hp on gastric. Anything else is ordered by path. u need to be
careful with ins. if neg the may not re-emburse.
Sent from my iPhone
On Jun 9, 2014, at 9:14 AM, kathy.mach...@lpnt.net wrote:
I was wondering if other labs are automatically ordering special stains
and/or IHC on
Leica all the way 2025
Sent from my iPhone
On Jun 2, 2014, at 5:19 PM, Sylvia Shockey
sylvia.shoc...@clinicalpathologyassoc.com wrote:
Hello,
My name is Sylvia Shockey I work in a histology lab. One of
our microtomes is not working properly and would appreciate some
Sorry 4 ur issues we use order entry in copath which is done at the path desk
the techs than call 4 a log print and work from that logs r held 4 1 month
Sue
TJUH
Sent from my iPhone
On May 6, 2014, at 1:18 PM, Dennis Hahn dennis.h...@cookchildrens.org wrote:
Currently, our histology lab
Not a good idea
Sent from my iPhone
On May 3, 2014, at 12:10 PM, John Smallwood jrsmallw...@bell.net wrote:
Our small Hospital with growth plans, is considering a new Laboratory in
the basement of the planned tower. I consider this a less than desirable
location. Spills , fumes,
. If you want you can call my cell 856-905-1549.
Sue Paturzo
TJUH
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We have the same issue. It is bad when they try to use the same QA form for out
of range
proficiency testing. a +3 or +4 is IHC is not the same as 70 or 200 glucose
result. The
pathologists are haven real issues with it as well.
S. Paturzo
So I went to one of the CAP webinars regarding competency And when I asked
what about
histology there were complete silence on the other end of the line and than
they said there was
nothing for histology.
At our site they want something for very division so I put together a basic
Dissection for frozen sections is a high complexity task, there may be an issue
with the
histologist. I am not so sure about usnig telepathology for the pathologist to
ID where to
take the section. This is touchy and I would hate to put the tech in the
position if there
was an error.
just get the coding book
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We had this issue when we were using the tape from Mercedes Medical and went
back to
Sakura tape and it went away. It could also be associated with humidity.
STP
TJUH
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We actually cut 2 slides with 4 levels 2 on each slide. Out pathologists were
unwilling to only
cut one slide
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Dragon use it with copath no issues
Sent from my iPhone
On Jan 30, 2014, at 2:21 PM, Rodriguez, Arnold arodrig...@emc.org wrote:
Which are the best digital dictation systems currently available for
grossing? Our LIS is CoPathPlus but they don't have a dictation module.
Arnold Rodriguez,
Joelle
Check CAP questions again, I am going to as well. We got caught on the two
identifiers for
frozen section slides, made all kinds of changes, but when we checked our new
questions the
questions was gone. We have not gone back to our old ways but just an FYI.
Susan T.
Has anyone used a disinfectant other than Lysol IC to do the decon on the
Ventana Ultras?
Thanks
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edumailto:shun...@beaumont.edu
Beaumont Hospital, Royal Oak MI. It's great!
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu
We do the same process as Linda does here at Beaumont.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun
Good morning
Does anyone have suggestions for antibodies for M2D2 and SMAD? We have not
been able to get these antibodies to work for us on the Ventana Ultras.
If you do have suggestions, protocols and control tissue suggestions would also
be appreciated.
Thanks
Sue
Sue Hunter, Supervisor
Try Victor Wong, Autostainer Technical Solutions 888-505-9545 cell
646-378-9222 www.autostainertech.com
Not sure if he is still in business, he was located in New Windsor NY
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun
Ventana Ultras and they are busy
all the time. and so are my 2 techs.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun
and exceptional service to
the NSH.
Please join us in congratulating Peggy on this well deserved recognition and
award.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edumailto:shun...@beaumont.edu
- this will be a really nice addition to the reviewing process. I
believe there are many laboratories where the cytotechs read out the urovsyions.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun
We always validated our processor but not formally. CAP wants a more formal
approach with procedures.
What we did in the past was run placenta and colon and reviewed stained slides.
We may have to be a little more critical and run various types of slides and
programs.
Susan T.
, (and use tap water) so I
don't think that is your problem. We are using the cheaper Fisher Superfrost
plus slides and they seem to work as well as the full priced ones. We cut our
immuno sections at 3um.
I know how frustrating this can be - I feel your pain!
Good luck!
Sue
Sue Hunter, Supervisor
to get consistent staining here. As they
say, The Enemy of Good is Better. Wish Leica had not made it better! It was
working very nicely before.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message
. The magic number seems to be
4 slides to get acceptable staining, but Leica suggests we load it up. We had
never had a problem until the upgrade in software.
Sue
-Original Message-
From: Houston, Ronald [mailto:ronald.hous...@nationwidechildrens.org]
Sent: Wednesday, June 12, 2013 10:59 AM
to
running them on the Ultra.
Just my opinion.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu
RNA technique.
If you are making up your own master mixes and primer mixes, be sure to use
RNAse free water.
Good luck
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun
filling with
water.
Sue
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Tuesday, April 02, 2013 5:36 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Out of my comfort zone
parameter of pressure to factor in to bring the solution
to a boil.
Good luck - hope it works for you.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
it as there was no interest by our clinicians.
If you ever find an antibody, please let me know. I would be interested in
trying it also.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun
Thanks for the update.
sue
From: Jim Burchette [mailto:jburc...@gmail.com]
Sent: Friday, March 15, 2013 11:02 AM
To: Sue Hunter
Cc: Zimmerman, Billie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: SOS EGFRV3
Sue, you are correct, EGFR v III was developed at Duke by Dr Darell
We use Ventana's ready to use for the Ultra, but it is clone KP1. You can get
the same clone in a concentrate from Cell Marque.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun
.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman,
Billie
Sent: Friday
only test FFPE tissues.
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Susan Foreman
We use xylene free processing + microwave
Formalin, 100% ethanol, isopropanol (clearant) and paraffin and have been doing
so for 4 years
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
. You will have to try
dilutions and time - usually 10 minutes is good, but with a diluted solution
you may need to go longer. Then wash the slides in PBS to get rid of the H2O2.
Good Luck
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun
need more material.
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Orsini
Sent
It is my understanding that you can only bill for one 88342 on a specimen -
technical or professional, even if you perform the same stain on multiple
blocks of that specimen. For A1, A2, A3 etc you can only charge once. For A1,
B1, C1, etc you may charge a 88342 per specimen.
Sue
Sue Hunter
long time and
gives off a nice bright light.
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf
Can you use the 3M freezing fluid in a histobath instead of isopentane?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
abri...@brightinstruments.com
Sent: Tuesday, April 10, 2012 6:46 AM
To: Bob
the reference lab? Would greatly appreciate hearing
from you concerning any processes you follow or insights you might have. Thanks!
Sue Breckenridge, Histology Supervisor
Cape Regional Medical Center
Cape May Court House, NJ
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pretreatments on each rack because of
timing/heating issues. But we have not found either of these two issues to be
a problem for us. The Bond III is supposed to be even faster than the Bond Max
but haven't looked at that.
Sue
Sue Hunter
Supervisor, Advanced Diagnostics
Beaumont Health Systems
that take
longer than the immunos.
sue
-Original Message-
From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com]
Sent: Thursday, April 05, 2012 10:51 AM
To: Sue Hunter; Wellen, Terrence D. :LPH Lab; histonet@lists.utsouthwestern.edu
Subject: RE: Leica Bond IHC Platform
We have
Wilson,
If you have access, read this article in the Journal of Histotechnology, Volume
34, Issue 2, pp 65-68, Gastrointestinal Biopsies: More Action and Less
Chatter?. It's a good discussion about improving section quality of small
biopsies.
Suggestions included:
Make sure your processing
We're in the process of switching over to the Bond III. For users of Leica's
Bond, what is your favorite decal solution to be used for ISH kappa and lambda?
Thanks in advance.
Rick Boen BS, HTL(ASCP)
St.Luke's Hospital
Duluth, MN
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Histonet mailing
Full time opening in East Liverpool, Ohio.
Opening is for a working Supervisor in a 1 person department.
Must be HTL certified with 5 years experience in histology, and
experience in being the in charge person.
Profiecient in all aspects of histology, accessioning, microtomy,
HE staining,
Hi All,
Now that MTM has made P16 very expensive and TriPath is have problems
producing ProExC, what's everybody using on ffpe tissue? We were told by
TriPath/BD that ProExC will probably not be available until late June.
Rick Boen, BS, HTL(ASCP)
St. Luke's Hospital
901 East 1st
until the sample has
been corrected by the sender.
For us; LEAN concepts/management is the preferred tool and then instrumentation
selection is layered over our LEAN environment that interfaces with our LIS.
Regards
sue
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I like Beatrice's method but I think it would only work in a small setting
where you are not
getting multiple frozens at one time as well as frozens that have 30 or 40
parts or working
with multiple pathologists.
Susan T. Paturzo
TJUH
___
I just ordered and received wash vessels from Leica last week. The order
number was 14045635268
The cost of each wash vessel was $165.00. I received them in about 8 days.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We have automatic stainers, so after the stainer is set up a slide with a
micro-array is run. This slide is
checked by the histologists and logged in. The next slides run are our rapid
cases. A log sheet is
prepared and handed to the pathologist with the slides and they are graded for
We do three levels on all diagnostic biopsies. Liver and Kidney also get
upfront special stains, as do gastric biopsies (h pylori)
We did cut down on extra unstained slides since we were discarding most of
them. As far as stopping levels, my pathologist thinks it goes against standard
of care.
I happend to disagree, it does not take much time to close cassettes and I feel
that the person grossing the specimen
should be responsible for what is in the cassette. If that cassette is found
empty the following morning who do you go to. You are stuck
with a he said she said.
We also use
Hi;
Can someone please explain to me what is meant by tamponated formaldehyde ?
Thanks
Sue
Sue Reilly
Histechnologist. Histology Unit. School of Marine Tropical Biology.
James Cook University,Townsville. QLD. 4811. AUSTRALIA
P (07) 4781 4181
F (07) 4725 1570
E sue.rei...@jcu.edu.au
Does anyone currently using Meditech version 5.62 for slide and/or
cassette labeling (via vendor slide/cassette labeling devices)? If so
could you please contact me? I am interested in other's experiences.
Thank-you,
Sue Breckenridge, Histology Supervisor
Cape Regional Medical Center
Cape May
Wondering what are your thoughts on chilling paraffin blocks via a
tissue freezing spray (e.g. ones used to further chill frozen sections)?
Thanks,
Sue Breckenridge, Histology Supervisor BS, HT/HTL(ASCP)
Cape Regional Medical Center
Cape May Court House, NJ
Confidentiality Notice
Breckenridge, Sue would like to recall the message, chilling paraffin blocks
question.
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