Re: [Histonet] tissue cassettes

[Thomas Podawiltz via Histonet]

Without seeing the blocks, that sounds more like cold molds being used, more 
Then, whether or not the tissues are kept in a dry, hot, well, or a wet well.


Sent from Yahoo Mail for iPad


On Friday, February 9, 2024, 6:00 AM, Brazie, Jeneanne E *HS via Histonet 
 wrote:

Hello :) I am encountering push back in our lab when I fill the embedding units 
with melted paraffin
in the embedding wells. The techs here like for the tissue cassettes  to sit 
dry (no wax) while in the
 embedding units. I find that the tissue rolls out of the sections while 
cutting because of a layering
effect between the tissue and the paraffin its embedded in. I have communicated
this but they tell me I'm "old school". Does anyone have any thoughts or 
opinions on this topic??

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Re: [Histonet] [External] Expiration date after opeing a reagent

[Thomas Podawiltz via Histonet]

I think that inspector has their head up their……….


Sent from Yahoo Mail for iPad


On Friday, July 21, 2023, 4:45 PM, Bitting, Angela K. via Histonet 
 wrote:

This sounds absurd to me.

-Original Message-
From: Paula via Histonet 
Sent: Friday, July 21, 2023 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Expiration date after opeing a reagent

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Hello,



We just got CLIA inspected and had no deficiencies. The inspector did say he 
wants to see us write down on any reagent containers when it was opened, and 
write down the new expiration date.



The containers have the expiration date on them from the manufacturer, but the 
inspector said when the container is opened, the expiration will change and we 
need to write the date on the container under the opened date.



I've never heard of this requirement and I would like to ask if you have.
He said if the vendor does not give us any written documentation of when a 
reagent would expire after it's opened, then we should seek an alternate vendor 
who can.



Any comments, etc. are greatly appreciated.



Paula Lucas

Bio-Path Medical Group



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Re: [Histonet] Biopsy formalin fixation times

[Thomas Podawiltz via Histonet]

Too general of a question. What type of biopsy? Minimum of 6 hours, maximum of 
72 hours for breast. 


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On Wednesday, June 14, 2023, 11:03 AM, Verno, Victoria R. via Histonet 
 wrote:

Good morning everyone,
        I have a question on biopsy formalin fixation time. How long should we 
let the biopsies set and fix in formalin before we gross/process them? You 
response is greatly appreciated.

Victoria Verno HT ASCP
Histology Supervisor of Hampton Roads
Desk: 757-398-4763
Fax: 757-397-7475

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Re: [Histonet] Coffee at the desk

[Thomas Podawiltz via Histonet]

 OSHA: bloodborne pathogen standard.A clean area is an area that is closed off 
from the working environment with walls and door. Tape is not a appropriate 
alternative, maybe on WKRP in Cincinnati, but not in a lab. 
On Thursday, June 1, 2023 at 03:40:41 PM EDT, Hannen, Valerie via Histonet 
 wrote:  
 
 Is there any regulation/ reason why a Histology supervisor whose desk is in 
the department and the desk area is taped off and designated as a "clean area ( 
 meaning no chemicals or specimens cross the taped off area) can't have a 
closed coffee cup at their desk??  We are having a debate in our Lab and I 
wanted to get a consensus.

Thanks in Advance,

Valerie

Valerie A. Hannen,MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Avenue
Titusville, Florida 32796
P: 321-268-6333  Ext. 7506
F: 321-268-6149
valerie.han...@parrishmed.com
www.parrishmed.com

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Re: [Histonet] Tissue Processor Schedule Validations

[Thomas Podawiltz via Histonet]

I ordered small punch biopsy kits and made my own small biopsies from remnant 
tissue. Renal biopsy kits worked for making our own needle biopsies. Leica 
already has the protocols that work so part is easy. 


Sent from Yahoo Mail for iPad


On Thursday, March 23, 2023, 3:05 PM, Normington, Lacy via Histonet 
 wrote:

We have purchased several disposable core biopsy instruments of varying gauges 
and take samples of large resection specimens. We also use the same grossing 
tools to take small samples of GI, endo, ecc, ect.

Lacy
 

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 1:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] IHC gunk

[Thomas Podawiltz via Histonet]

Is it on all slides or just certain antibodies?


Sent from Yahoo Mail for iPhone


On Wednesday, March 22, 2023, 1:57 PM, Willis, Donna G via Histonet 
 wrote:

What instrumentation are you using.  Have you talked to your vendor?

Donna Willis
Anatomic Pathology Manager
Baylor Scott Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



-Original Message-
From: Karen Heckford CA-San Francisco via Histonet 
 
Sent: Wednesday, March 22, 2023 12:45 PM
To: Histonet 
Subject: {EXTERNAL} [Histonet] IHC gunk


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Good Morning,
I have recently developed a problem with contamination of some kind on my IHC 
slides.
The contamination is black clumps and lays on top of the tissue.  I have
been told it is bacteria but the Pathologist and I kind of doubt that.  I
cannot get a good picture of it to show.  It may not be on all the slides on 
the same run or even the same antibody slide.

I have decontaminated the whole system.  I have put fresh reagents on the
instrument.  This stuff looks like it would wash off at the end of the run
since it is sitting right on top of the tissue.  I do not see this stuff
elsewhere on the slide, only the tissue.  Does not matter if the tissue
was cut fresh or not.

I have tried everything I can think of to get rid of it and still have this 
issue.  I use DiH20 from the hospital system.  Not sure if this may be the 
problem.  I can have Engineering test the DiH20.

Any help would be greatly appreciated.

Thanks,

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-750-5751

karen.heckford@ commonspirit.org




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Re: [Histonet] Testing for hires

[Thomas Podawiltz via Histonet]

Because of legal issues of having non employees using our equipment I never had 
them cut or embed. I would give them several blocks and slides and ask them 
what was wrong with them. 


Sent from Yahoo Mail for iPhone


On Wednesday, November 16, 2022, 1:24 PM, Anne Murvosh via Histonet 
 wrote:


Melt down old blocks, have them embed and cut them. Evaluate their time and 
technique. You could also do a working interview where a tech shows them around 
but has them cut and embed a few current tissues while watching. Thanks Anne

Anne Murvosh
Histology Technician

                            Three Locations to Serve YOU!
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83814

509-456-7414  Office
509-624-0763  Fax
amurv...@advancederm.net
http://www.advancederm.net

-Original Message-
From: Stephanie L. Thompson via Histonet 
Sent: Wednesday, November 16, 2022 10:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Testing for hires



When hiring for a histotech, how do you test their skills?

Thank you,

Stephanie
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Re: [Histonet] Molds- cold vs warm

[Thomas Podawiltz via Histonet]

Tell us how you really feel Jay. LOL. 
You say everything that I would say and have said while training people. 
Like you having been doing this for a bit (1980) and had  the privilege of 
meeting Mr. Lee Luna. 

 


Sent from Yahoo Mail for iPad


On Friday, September 9, 2022, 6:14 PM, Naira Margaryan via Histonet 
 wrote:

Thank You so much Jay, for such detailed explanation and for permission to
use your email to address.

My sincere regards,
Naira

On Fri, Sep 9, 2022 at 5:00 PM Cooper, Brian  wrote:

> Thanks for saying this Jay!! I have to say, it's been a while since we've
> had such a great response on Histonet!! Everything you said is spot on.
>
> Happy Friday everyone!
>
> Thanks,
>
> Brian Cooper
> Histology Supervisor
> Children's Hospital Los Angeles
> Sent from my mobile
>
>
>
>
>
> On Sep 9, 2022 2:37 PM, Jay Lundgren via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> CAUTION: BE CAREFUL WITH THIS MESSAGE*
> This email came from outside CHLA. Do not open attachments, click on
> links, or respond unless you expected this message and recognize the email
> address: histonet-boun...@lists.utsouthwestern.edu.
>
>
> Whoever is telling you to use cold molds needs to go back to clown college.
>
> That is totally, 100%, absolutely, wrong.
>
> There is some debate as to embed "wet" (cassettes submerged in paraffin
> bath) or "dry", and I will accept either, as mostly a matter of personal
> preference.  BUT, in both of these cases, the molds are hot.
>
> I have been a Histotech for five decades, trained at Armed Forces Institute
> of Pathology (back when that used to mean something) and I have NEVER seen
> anyone using cold molds.
>
> It is a guaranteed way to get cold fractures and cracks in your blocks, or
> to pop tissue out when you are cutting, which might be irretrievable. Just
> think how much time all those re-embedded blocks are going to save you!
>
> Also, you won't be able to easily re-position specimens in the block, to
> put them "on edge" or "on end", for example.  The tissue will instantly
> stick to the cold mold.  And if you want to re-position it, guess what,
> you'll have to warm the mold up to get the tissue unstuck.  How's that
> (non-existent anyway) time savings now?
>
>
> If you want to prove to whatever jackass suggested this that they are
> wrong, get a big stack of every histopathology textbook you can find.
> There is nothing in any of them talking about paraffin embedding with cold
> molds.
>
> As a matter of fact, every single textbook will specify molds at the same
> temp as paraffin.
>
> Anyway, it doesn't even make sense, thermodynamically.  Heat travels from
> hot to cold.  Those "cold" molds will be the same temperature as the
> paraffin, almost instantly. Did it take a tiny amount of heat out of the
> hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The
> amount of heat from the paraffin used to warm the mold is trivial compared
> to the total heat of the system. That's why cold plates have huge, noisy
> refrigeration units.  You can't argue with thermodynamics.
>
> If you are having trouble getting your blocks to release, use mold
> release!  Viola!
> https://secure-web.cisco.com/1vhENcmRngDgLubdLEYMzzWWUK4ILg_WIJNnMutz67Oikk5LSg5SqF6OvSQqMWpr4MIirbF_ExGbIXm9Usdm35LUk87pXYTIvPVdKY5u2dRCdo_Ss-iuZ4nCOa0nPTIpPec8zwvOBcVIE7eM7o-flt9BAIGK0ZOw4K3HOXwNiLmQBnD0hFb9pgrU0ZuPnk5llOYCeJ5b2Pmkp2B9UPlVvxPMI3-iHRILtOB4kPL45PII_yUJnJhFYAryeid5lrITtm-w0KNyKrfJVI0mHy47Niz0TEpxxvl3DoTDmq-umsyN3BucCj2B-aJFqJ-AW3thtXSEk-Nl0NzBBrSxw8cPzSrKsVww7cCLh_krbh7VXKlRiRGF41o3UKk_oEQuHGIEeYlUNLnpLndnkSH0cwR3nNWhq3Cy8hw6ws0Ka8kYRH8_TVttsOh_lQbO4tm6_i-fdNOZxcR_7t-QeE9aW5YP1hg/https%3A%2F%2Fwww.statlab.com
> I used to think it was
> superfluous, but now I consider it compulsory.  This is probably the answer
> to most of your issues.
>
> I don't know who is suggesting using cold molds, but I can pretty much
> guarantee that it's a pathologist who thinks his slides are taking too
> long, and knows nothing about histopathology, or a lab manager, who knows
> nothing about histopathology.  This next part is directly to them.
>
> To Whoever Suggested Cold Molds:  The answer to getting your slides out
> quicker is buying more equipment and hiring more techs, and holding
> everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding).
> Making nonsensical, uninformed suggestions only exposes your ignorance.
>
> Please feel free to show them this reply.
>
> Sincerely,
>
> Jay A. Lundgren, M.S., HTL (ASCP)
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> 

Re: [Histonet] tissue disposal

[Thomas Podawiltz via Histonet]

We dumped tissue two after the final report is issued and toss the containers.


Sent from Yahoo Mail for iPad


On Wednesday, October 13, 2021, 3:36 PM, Richardson, Pam K via Histonet 
 wrote:

I am interested to learn how others are handling tissue disposal. How long to 
you keep wet tissue? Are you reusing containers? We currently dispose of the 
tissue and wash the containers and I am questioning if there is a better 
system? 

Best Wishes

Pam ~
National Histology Professionals Day 3/10/21
Pathologists' Assistant Day 4/14/2021
Medical Laboratory Professionals Week April 18-24, 2021
National Cytotechnology Day 5/13/2021

+++
Pam Richardson
Clinical Manager
Gundersen Health System Laboratory Services
Email: pkric...@gundersenhealth.org
Phone: 608 775-4133
Fax: 608 775-6136
Interdepartmental Mail Stop: H04-007
E-visit us at: http://www.gundersenhealth.org

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Re: [Histonet] Filed slides sticking together

[Thomas Podawiltz via Histonet]

I never have. Usually let them sit for two days before we filed them.


Sent from Yahoo Mail for iPhone


On Friday, April 16, 2021, 10:05 AM, Martha Ward-Pathology via Histonet 
 wrote:

I am posting this question for our Histology manager:

Does anyone dry coverslipped slides in an oven before filing and if so how long 
and at what temperature.  We are having issues with filed slides sticking 
together.


Thanks in advance for your help with her question.

Martha  Ward, MT ASCP (QIHC)
Manager, Molecular Diagnostics Lab
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157

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Re: [Histonet] Processing correction

[Thomas Podawiltz via Histonet]

Recovering Tissue That Has Dried Out After Malfunction of Tissue Processor 
  
|  
|   
|   
|   ||

   |

  |
|  
|   |  
Recovering Tissue That Has Dried Out After Malfunction of Tissue Processor
 

  |   |

  |

  |

  


Sent from Yahoo Mail for iPhone


On Monday, March 1, 2021, 2:10 PM, Charles Riley via Histonet 
 wrote:

We had an issue last week where the tech loading the processor forgot to start 
the machine. The tissues we left in an empty chamber for around 8/9 hours after 
having been in formalin from anywhere from 3 hours to 10 hours from the 
surgical sites.

All of the specimens are unreadable. What steps can be taken (if any) to try 
and salvage the samples and get better slides for the pathologists to read?
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Re: [Histonet] FW: Tissue disposal

[Thomas Podawiltz via Histonet]

 After decanting the formalin off our tissue is bagged in biohazard bags that 
are in a biohazard box. The box is labeled pathology waste for incineration 
only. 



On Wednesday, January 29, 2020, 02:52:17 PM EST, Rathborne, Toni via 
Histonet  wrote:  
 
 
Stericycle will also take your blocks and slides when you're ready to discard 
them.

Toni 





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-Original Message-
From: Carol G Fields via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 29, 2020 2:05 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] FW: Tissue disposal


*** This is an External Email ***

FYI…_
From: Carol G Fields
Sent: Wednesday, January 29, 2020 8:32 AM
To: 'Eileen Akemi Allison' 
Subject: RE: [Histonet] Tissue disposal


Hi,
Stericycle takes care of all your waste, including decanting and disposal.  
They are all over the US..Health & Safety alone plus employee time will justify 
you using these people.  No one should still be doing this…
I’ve used this company at several hospitals. They are great.
Good luck,
Carole
MLKCH
Los Angeles, CA

https://urldefense.proofpoint.com/v2/url?u=https-3A__www.stericycle.com_landing-2Dpages_minimal-2Dglobal-2Dwrapper_regulated-2Dmedical-2Dwaste-2Ddisposal-3Futm-5Fcampaign-3DSEM-5FOngoing-5FTopCoreExact-26utm-5Fsource-3DGoogle-26utm-5Fmedium-3Dcpc-26utm-5Fcontent-3DB-5FTop-5FCore-5FG-5FSEM-5FTxt-5FExact-26utm-5Fterm-3Dstericycle-26gclid-3DCjwKCAiA98TxBRBtEiwAVRLqu8nk2kjv5k4J-2Dd-5Fzb9menIfZRowLKKJY4Df3abYLkdk0xS02OKgFBBoC8k8QAvD-5FBwE=DwIGaQ=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g=K-6G4PgDF8DqhiakFxI1pnea8lR2C9xQHqWnrYHUefk=imWJ8sVczAToQjB1FZDf_-zlB9uXzpNibMHeYlXCXqs=

Stericycle is a compliance company that specializes in collecting and disposing 
regulated substances, such as medical waste and sharps, pharmaceuticals, 
hazardous waste, and providing services for recalled and expired goods. It also 
provides related education and training services, and patient communication 
services. Wikipedia
Stock price: SRCL (NASDAQ) $63.65 +0.11 (+0.17%)
Jan 29, 10:57 AM EST - Disclaimer
Headquarters: Lake Forest, IL
CEO: Cindy J. Miller (May 2, 2019–)
Number of employees: 23,200 (2017)
Founded: 1989
Subsidiaries: Shred-it, Stericycle Environmental Solutions, MORE



-Original Message-
From: Eileen Akemi Allison via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, January 29, 2020 4:25 AM
To: Histonet 
mailto:Histonet@lists.utsouthwestern.edu>>
Cc: mirnaiba...@umcelpaso.org
Subject: [Histonet] Tissue disposal

Good morning Histopeeps! After reviewing our Policy Procedure Manual, our 
manager is questioning the bags we are using for disposing our tissues after 
diagnosis is complete.  We are separating the tissue from the formalin by 
straining the excess formalin and neutralizing it.  We then put the tissue into 
“Large Biohazard Bags” and the EVS staff picks it up and disposes it.  My 
manager is wondering if the “Biohazard Bags" are appropriate.  Should the bags 
state “Tissue” on the them?  Are there any special disposal bags available?

While working for a GI Lab in Monterey, CA we put all our bottle and tissue in 
"Biohazard Bags" and a Hazardous Waste company picked up the waste products 
from our endoscopy department along with ours.  Any comments and suggestions 
would be greatly appreciated.

Thank you in advance,
Akemi Allison, BS, HT/HTL


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Luther King, 

[Histonet] Open Position

[Thomas Podawiltz via Histonet]

I have an open 2nd shift position. 
Hours are 2 to 10:30 pm Sunday to Thursday. 
Concord Hospital
Concord, NH. 
If interested contact me direct at tpodawi...@crhc.org
Thank you, 

Tom  PodawiltzPathology Supervisor Concord HospitalConcord, NH. 
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Re: [Histonet] scrubs/business casual

[Thomas Podawiltz via Histonet]

 blockquote, div.yahoo_quoted { margin-left: 0 !important; border-left:1px 
#715FFA solid !important; padding-left:1ex !important; background-color:white 
!important; } We allow everything from business casual, scrubs to neat blue 
jeans. But then again we are on nights.


Sent from Yahoo Mail for iPhone


On Wednesday, June 21, 2017, 7:33 AM, Haley Huggins via Histonet 
 wrote:

We wear scrubs. However, we are a small reference lab and our employer
bought us 4 sets of scrubs w/embroidered company logo to wear M-Th. I added
free-scrub Friday, so we can wear whatever scrub set we want to wear on
Friday purchased on our own.

On Jun 21, 2017 6:51 AM, "O'Donnell, Bill via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

Just a little poll.

How many histology labs allow scrubs?

Of those that are allowed scrubs, do you purchase your own, or are they
provided?

Bill O'Donnell
Good Sameritan Hospital
Histology

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