[Histonet] Harvard Brain Bank Job Opportunity
Hi Everyone: Well, at the age of 67 and after more than 30 years of service, I am retiring from the Harvard Brain Tissue Resource Center (the Brain Bank) at McLean Hospital in Belmont, Massachusetts. My last day is today, December 30th 2016. We are looking for someone to take over my position. If you are interested, please click on the following website. http://www.mcleanhospital.org/apply-work-mclean This website brings up the different positions currently open at McLean Hospital. The position is "Histologist Lab Manager - On Call" and the Job Number is 3029453 To find out more about the Brain Bank, you can also check out our website by searching for "Harvard Brain Bank". This will take you to our home page. I hope that everyone is having a wonderful holiday season. Tim Tim Wheelock Neuropathology Laboratory Harvard Brain Tissue Resource Center McLean Hospital, Belmont, MA 02478 Brain Bank general number: 1-800-272-4622 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mats
Hi Mala: We use the following: PIP Technical Contamination Control Mat Vendor: Fisher Catalogue #: 19-122-822 They work very well; just peel off the top layer, and you have a fresh one ready for the next round of sectioining. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA -Original Message- From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 12:08 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] mats What is everyone using as floor mats in histogy. The mats we have are very thick and with Holes, we are finding it difficult to move the chairs on them to get close to the microtome for cutting. We were told that carpet of any kind cannot be use. If we remove the mats, the floors are very slippery. Any idea will be greatly appreciated. Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Salary range
Hi Everyone: First thank you for letting me know the designation of someone who is certified in immunohistochemistry. I have a second question. Do you have an idea of the average salary range, in the Boston area, for someone who starts a job managing a pathology or neuropathology laboratory (by managing, I mean being responsible for all the functions of the laboratory, while working alone), if they are a new employee, have an HT/HTL and QIHC certification, and have 3-4 years of experience in a pathology or neuropathology laboratory? Thanks so much, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Immunohistochemistry
Hi Everyone: What is the designation of someone who is certified (registered) in immunohistochemistry? Thanks, Tim Wheelock Harvard Brain Bank Mclean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Distilled Water vs DI water for IHC and H staining
Hi Charles: Many years ago I was using deionized water to do the Bielschowsky silver stain. The stains were coming out way too dark, with black-brown precipitate on the sections. When I inherited a double-distilled water filtration system, that problem disappeared. However, silver stains tend to be sensitive to all sorts of things. I don't know if the same holds true for immunostains, since I don't do them myself (another lab does them for us). When I do my Luxol Fast Blue (myelin)-Hematoxylin-Eosin stain, I just use tap water or all my washes. I make my solutions for the stain with double-distilled water, but I am not sure if that is necessary. I hope that this helps. Tim Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA -Original Message- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 28, 2016 7:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Distilled Water vs DI water for IHC and H staining Does anyone know if there is a difference in staining results when using DI water versus Distilled water? Our lab is trying to get away from the DI system we have as it is becoming expensive to maintain and we were looking to see if we could just use a home drinking filter with UV lighting for our water supply. Please let me know any thoughts or concerns you might have with this idea -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Course of digital imaging?
Good morning everyone: I have a DP-70 Olympus digital camera. Recently, I upgraded to a cellSens Standard software for the camera. Although some of my images, taken with the old Olympus software were gorgeous, many were not. I am also having problems with the new cellSens software. I feel that I need a real class or course on taking good microscopic digital images from histologic slides. Does anyone know of such a course, preferably in the Boston area? A course on the optics of a microscope would not hurt either. Thank you, Tim Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital, Belmont, MA Phone: 617-855-3592 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Over-processing of brain tissue
Good morning everyone: I seem to be having problems with over-processing of brain tissue. I use a VIP6 processor. I start off with 1 hour each of 30%, 50%, 80%, and 95% isopropanol. Then half-hour each of three changes of absolute isopropanol. Then half hour each of three changes of xylene. Then half-hour each of 4 changes of Paraplast. I use a slow mixing cycle and no vacuum-pressure for all reagents except the paraffin. For the paraffins, I use vacuum pressure, but no mixing cycle. I rotate the paraffins during each run of tissue. I replace the dilutions of isopropanol after I have processed 500 blocks. I rotate the absolute isopropanols and xylenes after 500 blocks as well. When I embed the brain tissue, it sometimes seems a little stiff, or even a bit brittle, to one extent or another. When I trim the blocks, they seem somewhat dry. Once in a while there is even a saw-dust effect. Before I section the blocks, I have to keep them on ice for at least 3 hours before they are moist enough to cut. Even then, the first case (out of 5 cases) shows chatter in the cerebral cortex. When I examine the stained sections microscopically, even the best sections look a little "rough" or dry. Taking microscopic images can be difficult at 40x ( or even 20x) because all this roughness shows. The brain tissue looks "granular" rather than smooth, especially with a LHE stain. I have continued to reduce the times to their present values, but it still does not seem enough. I absolutely love the VIP6, but it is a much more powerful machine than the Shandon Hypercenter XP that I use to have. Perhaps I have still not fully compensated for this power. It has 4 paraffin reservoirs rather than 2 on the old Hypercenter, and the reagent reservoirs hold twice as much volume. Any ideas on how to resolve this problem? Should I reduce the times further? Should I alter the use of the mixing and/or vacuum-pressure? Thanks for any help that you can give me. Tim Tim Wheelock Assistant Director, Neuropathology Instructor of Neuroanatomy Tour Coordinator Harvard Brain Tissue Resource Center Room 203, Mailman Research Center McLean Hospital, Belmont, MA 02478 Phone: 617-855-3592 Cell: 857-234-9311 Fax: 617-855-3199 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microscopic slide files
Hi Rayen: We buy our stackable slide files and slide file base from Fisher Scientific: Here are the items and catalogue numbers, which are for tan-colored files. Other colors are available. Microscopic slide file drawers 07-212-100 Microscopic slide file drawer base 07-212-104 Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Favorite book for histotechnology and immunohistochemistry?
Hi Everyone: I have a co-worker who will be starting in a surgical histology laboratory soon, and wants to get his HTL. What is the best all-around book of histotechnology for him to have? Also, is there a favorite book for immunohistochemistry? Thanks, Tim The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temperature and Humidity check
Hi: Yes, I check both temperature and humidity as part of a morning " lab systems check". This also includes checking the fume hoods, distilled water, emergency eye wash, refrigerator and oven temperatures, the tissue processor clock, and embedding center and water bath temperatures. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont Center The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Solvent recycling
Hi Everyone: My hospital recently has been looking into recycling solvents. Our department alone does not have the volume to justify such an expense. However, there are many research labs on the campus that produce varying (probably small) quantities of solvent. They have asked me to look into the issue. When I was working in a large surgical histology lab many years ago, we never had any solvent recycling, so please forgive my basic questions. When I contemplate this issue, many issues arise: How big would the apparatus be (I am assuming they come in different sizes)? How much do they cost? Where would it have to be located? Do different solvents require different recycling machines? Who would be responsible for the machine? If it were a shared instrument, it may increase the likelihood of malfunctions. How much preventative maintenance does it require? I am assuming that training would be needed for all users. How comfortable would we and researchers be in using recycled solvents, as opposed to just buying new reagents each time? Anyway, I would appreciate any advice on this issue. Thanks you so much Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens
Hi Everyone: It appears that for security and privacy reasons, the NIH wants us to change from an internal specimen tracking system that employees sequential numbers(8634, 8635, 8636 etc.) to a system that uses randomly generated anonymizing number (20487, 71936, 88011 etc.) It seems to me that this invites mistakes and mixing up of cases. (Humans seem to deal better with sequential numbers). This would include everything, from the buckets with formaldehyde in which half brains are fixed, to wax blocks, to slides, to block and slide files, to the images that I take on each case. Does anyone have experience using computer generated random anonymizing tracking numbers in their pathology or tissue banking departments? What system of checks do you employee to avoid mistakes and make the work go smoothly? Perhaps this system will work fine, once we are used to it. Thank you very much for any feedback. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anonymizing numbering system
Hi Everyone: Thank you for all your responses and thoughts on this issue. I should clarify that this new system may actually not be coming from the NIH itself, but perhaps from departments in one of our parent organizations. We have no written sources for this sort of approach. They may be understandably concerned about separating personal identifying information from clinical/pathological diagnostic information, so as to ensure confidentiality, especially in this day and age of increasingly vulnerable personal information. Assigning a randomized anonymizing number tracking system may help in this regard. I have been assured that a code, that is a way of matching the anonymizing number to a sequential system would be in place. So there would always be a way of getting our bearings at any point. My concern was mistakes and mixing up of cases. I still would prefer a sequential system, but at this point, we don't really have an option. Plus, maybe it is just that I am used to a sequential system. I think it may be difficult to get use to at first, but i f we take it carefully, and everyone cross-checks each other, it should be do-able. We shall see. Thanks again. Tim The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thank you
Hi Everyone: I want to thank everyone for the advice concerning the chemical sensors and badges. It is very helpful. Have a great weekend. Tim Wheelock The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cutting of Paraffin blocks
Hi Adesupo: In 1995, I had two 18 year old summer students from Strasborg, France. They were here in our neuropathology lab for 2 months. I had them cutting within a week or so after their arrival. By the end of the 2 months, they were processing, embedding, trimming, sectioning, and staining beautiful sections. All they needed was a careful safety orientation, a demonstration, and then practice. Hope this helps, Tim Tim Wheelock Harvard Brain Bank Mclean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problem with cracked paraffin blocks
Hi again everyone: I want to thank you all for your advice. The consensus is that I have my new embedding center's cold plate set way too low at -14C, and that I should try raising it to around -5C to cure my cracked block problem. I will run some test blocks over the weekend, and then embed them at this new temperature on Monday. Otherwise my Valida embedding center is working very nicely (as did the HistoStar when I demoed it). The cassette holding tank can accommodate a large number of brain specimens and the controls are very easy to use. Thanks again for your advice. Have a great weekend. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin-Plastic Stratification/Congo red problem
Hi Jeffery: Yes, I notice the same paraffin dust bunnies, perhaps especially since I use a embedding paraffin that has a fair amount of plastic. Before I embed my brain tissue, I mix the embedding media thoroughly, until the solution is clear. As for your Congo Red problem, I wish that I could help you. I am now experiencing the same problem, except that it affects the positive controls as well. The staining is there, but much fainter than it should be. I usually immerse albumin coated slides in 1% Congo red for 15 minutes, differentiate them with Potassium Hydroxide for 3 dips, counterstain with Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10 dips, immerse in running tap water for 10 minutes, immerse in 95% ethanol for 2 dips, then dehydrate and mount. Tim The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cracking paraffin blocks
Hi Everyone: Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. I am having problems now with blocks developing cracks on the cold plate. The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. I use Surgipath Embedding Media (EM-400). The surface of the Valida's cold plate is -14C. The company has not seen this happening before, but they are looking into this further. Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. I do not think that this is a problem inherent with any particular machine. Has anyone encountered this problem before? If so, how did you resolve it? Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly rougher texture. Could this produce a different way of conducting heat out of the paraffin block than the Shandon? Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? I have used the Shandon Embedding Center for over 24 years. I have never had a problem with blocks that crack. Thanks for any thoughts that you may have on this. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Congo Red problems
Hi All: Lately, I have been having problems with my Congo Red staining too lightly. The Bielschowsky silver stain shows lots of amyloid in the cerebral blood vessels and the cores of the senile plaques. However, the Congo Red stain is pretty faint, although you can still see it. I have just bought a new bottle of Congo red from Sigma, in case it was the particular batch of dye. My protocol, which I have always used, is as follows: 1. Deparaffinize sections and bring down to 95% ethanol 2. Stain in 1% Congo Red solution for 10 minutes. 3. Differentiate in Potassium Hydroxide solution; 3 dips 4. Rinse in 4-5 changes of tap water. 5. Counter-stain in Harris Hematoxylin for 1 minute. 6. Differentiate in Acid Alcohol; 10 dips or so. 7. Blue Hematoxylin in running tap water for 10 minutes. 8. 95% ethanol for only 3 dips, then right into absolute ethanol. 9. 20 dips in first absolute ethanol 10. 1 minute in absolute ethanol 11. Clear and mount. Any suggestions would be really appreciated, Tim Wheelock Harvard Brain Bank Mclean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HistoPro 150 Embedding Center
Hi Everyone: Has anyone had experience with the Rushabh HistoPro 150 embedding center? Could you share your thoughts and opinions about this machine? Thank you, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Location in the Lab
Hi Everyone: Even though many tissue processors are closed systems with fume filters, do people try to put them next to fume hoods or under some ventilation duct to add an extra layer of protection from solvent fumes? Thanks, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] isopropanol for tissue processing
Hi Julio: I have been using isopropanol for as long as I can remember. I work only with post-mortem brain tissue (in standard-sized cassettes) in a neuropathology laboratory at a brain tissue bank. Because the brains have been previously fixed in 10% neutral buffered formalin for a month, I do not have to use formalin on the processor. Anyway, I start out with 30% isopropanol, then 50%, 80%, 95%, 3 stations of 100%, 3 stations of xylene, then into paraffin. I cannot remember whether we used isopropanol in surgical histology years ago, when I was at another hospital, but I think we did. Good luck, Tim Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Julio Benavides Sent: Tuesday, August 19, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] isopropanol for tissue processing Hi, In our histology lab, we are considering to move from ethanol to isopropanol for processing formalin fixed samples (post mortem samples, so big samples). I have been diving in the histonet archives and have found that the subject has been laready discussed, but somehow I cannot find a definitive message stating Yes, we have tried and it works perfectly. This is our protocol. So here I am, writing the forum to see if anyone can kindly confirm that the move from ethanol to isopropanol is not a traumatic one, and if any of you has eliminated the xylene as clearing agent as well (and what have you used instead. Isopropanol/paraffin at 50c or straight into paraffin?) I have been speaking with Rene J Buesa who, besides a very helpful person, is highly enthusiast in the use of isopropanol. He has almost converted me to the isopropanol side! Thank you very much for your time and help Regards Julio Benavides Instituto de Ganadería de Montaña (CSIC-University of Leon) Spain ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
