RE: [Histonet] OT: Wednesday Half-Wit

We used to put a red bio-hazard bag (the heavy duty one's) into a cardboard box. This usually did not leak. Just saying if you still want to use your box method =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744

[Histonet] Vasculature in tumors

Hello all and happy long weekend (yes our boss gave us the rest of the day off at 2!!!) Looking to stain vasculature in tumors. I did trichrome, pentachrome, and PAS (with digestion) and am being told that these are not optimal for what they are looking for. Any other ideas to stain blood

[Histonet] ...maybe a little more specific

So am getting lots of responses for IHC stains (which is ok), but I am doing these stains on mouse xenografts and the background is getting in my way a lot. Was just trying to find a simple special that would do the trick =) If IHC is the only way, so be it...was just hoping... What about

[Histonet] Luciferase

Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list

RE: [Histonet] Luciferase

Someone just responded “only if it’s possessed” I laughed =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Angela Bitting [mailto:akbitt...@geisinger.edu] Sent: Thursday, June

[Histonet] PAS oops...

So I dumped my periodic acid like a moron...does anyone know of another reagent that will oxidize for the PAS reaction? Looks like I have acetic and hydrochloric acids...and that is all...hmm... Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street

RE: [Histonet] Tissue Shriveling in Paraffin

You could have got the tissue wet or to hydrated. Sometimes after you face the block if you leave it on the ice to long and let moisture get into the tissue then cut the slide, it looks ok at the time, but once the moisture gets back out of the tissue it will start to look concave. If you are

RE: [Histonet] RE: HistoGel

You can also use agar. It does the same thing and is cheaper =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] bluing

Just add a little bit more ammonia hydroxide to the water =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: Re: [Histonet] How many tissues an histo tech is suppose to cutper

Another solution...get out of clinical and go into research =) There are no quotas or slide per second expectations in the research world =) So sorry you are having such a bad time with your job. HT's are not a dime a dozen and usually it is fairly easy to find a better job. Not to mention

RE: [Histonet] How many tissues an histo tech is suppose to cut per

I second this motion!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]

[Histonet] Austin PRN

Hello all =) Since I just recently got married and still own a house we do not live in, I am finding myself in the pickle of 2 houses and not enough income...Does anyone know of any kind of part time histology jobs in Austin that I could go to either early until around 7am or late after 5pm? Or

RE: [Histonet] Retirement

The smoking thing is what I missed...I know I work in cancer research and I shouldn't smoke, but as I tell everyone...by the time I get lung cancer I will have helped to find the cure (wishful thinking and stupid excuse making I know.) Now almost everywhere I work you have to completely leave the

[Histonet] PAS

Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912

RE: [Histonet] Retirement

2035 huh?? Crap!! I won't be retired by then I'll be 54! Hmm...guess I need to invent the histo. robot so I can still have income!! =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912

RE: [Histonet] Keeping Histo room floor clean?

You can buy this type of thing too if you aren't the McGiver type. For instance...from American Mastertech item # CPW04200E Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original

RE: [Histonet] RE: Retirement

I'm 30, so blah blah blah...I have 30 or 40 more YEARS!! =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Vimentin

Does anyone have a picture of a positive control Vimentin on human tonsil? I'm getting some arguments... Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912

RE: [Histonet] Slide Bright Question

Ahhh!!! I had this same problem when I came to this lab too!! Plus if you put that xylene substitute crap (mine was clear rite) it screws up cell blocks that have been embedded into agar!! 5 experiments had to be repeated because of this stuff!! The eosin bleed is because you got water on the

[Histonet] Slides for IHC

So I just cut a bunch of slides for Ki-7...I am not going to be able to stain them until Monday. I know the best thing is to put them in 4 degrees, but will it hurt them to stay at room temp for 3 days? Putting them in the fridge gets a lot of moisture under them and they tend to lift more. Any

[Histonet] Tumors tumors everywhere

So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is

RE: [Histonet] Two Questions

Usually I think it has to have a door for fire purposes. Especially since there are so many flammable chemicals in the lab. I could be wrong? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912

RE: [Histonet] Problem with IMEB

I have had excellent luck and service with them. Call Denise deVines she is super nice and helpful =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From:

[Histonet] Positive control

Does anyone know what the positive control for MET is? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list

RE: [Histonet] endogenous peroxidases

So I have been seeing from this post that people block prior to HIER? I have always done it as the first step after for 10 minutes (I use the DAKO enzyme blocker). Is this wrong? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas

RE: [Histonet] eosin bleeding on frozen sections

This happened to me a couple weeks ago. You got water on the slide after the eosin staining. Just run it back through alcohols, and rinse for about five minutes. Change out the alcohols, and re-stain. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite

RE: [Histonet] decalcification of articular cartilage

You could always get formical which fixes and decals at the same time. This would eliminate the waiting for fixation. Although I do usually let it fix in straight formalin overnight first. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin,

[Histonet] Thank you all!!

So...I sacrificed a couple of the slides and stained with HE per ya'lls suggestion and...yay!! Cells all over the place. See I freaked myself out for nothing. Now, where is 5:00? =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin,

[Histonet] uh...

So I just stained a group of slides all at the same time with the same conditions. About 40 of 150 the eosin looks like it is bleeding out of the sections...this has never happened before? What could be the cause and how do I fix it? Everything is as normal, but again I am being forced to use

RE: [Histonet] National Average Salary

So apparently I'm underpaid =) Good Luck convincing employers of this! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] April 1st Fun

The funny part is I hear that can be a fungus control!! =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Stupid Rabbit primaries!

So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to

RE: [Histonet] Stupid Rabbit primaries!

I blocked for all of those... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Tuesday, March 22, 2011 4:13 PM To:

RE: [Histonet] Storing slides in buffer

I just did this last week. I stored them in wash buffer overnight in the frig. and they were fine =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From:

RE: [Histonet] Polymer detection kit

I use the Biocare polymers all the time because they are cheaper than DAKO. Some of them are biotin free, so as long as your primary has no biotin and you aren't staining anything with endogenous biotin you can omit that block. Other than that enzyme and protein blocking I still do. Some of

[Histonet] RE: cell blocks

I actually fixed the cells without the agar overnight, and left them in the agar overnight... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: Tony Henwood

RE: [Histonet] diastase

Just hack up some spit... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

RE: [Histonet] microtome safety

I use the end (without bristles) of a paintbrush Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] cell blocks

So...I am trying to make a cell block using 0.9% agar. I have done this in the past with no problems. It was at a different facility and I don't know if the agar is the same? I used bacto-agar this time, but last week someone tried to do my protocol with agarose and the boogers dried up and

RE: [Histonet] Fat tissue

For the most part you cannot section fat on a cryostat because of the oilyness (is that a word). You can fix it and section it? Good Luck Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original

[Histonet] IHC slides

So...I have run down slides and done antigen retrieval on my FFPE slides. They are currently in antigen retrieval that has come to room temperature. I am not going to be able to finish the IHC stains until tomorrow. Will it be better to keep the slides in the antigen retrieval solution at 4

RE: [Histonet] Telepathology

It's not nice to make fun of people's typing boo-boo's, but thanks for the Friday chuckle!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From:

RE: [Histonet] Polymer detection kit on rat tissue

I personally love polymers!! The DAKO envision is actually using polymers. You don't have to buy the kit. Biocare has a ton of awesome polymers for sale that I use in tangent with DAKO. If you want more specifics on my protocols let me know. Good Luck Sarah Goebel, BA, HT(ASCP)

RE: [Histonet] cdj

Ahhh!! Everytime I hear those letters I have flash backs to my hospital days!! Stupid mad cow disease!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From:

RE: [Histonet] CAP

What?!? A histology lab with no enough space...who ever heard of such a thing Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RRAS

So...trying to find someone who sells RRAS antibody that isn't Abcam. I can't seem to make the Abcam antibody work and I have been asked to try and get it from a different company. 2. Does anyone have any experience with RRAS? I have tried dilutions from 1:500 all the way up to 1:2000. I

RE: [Histonet] Formalin-fixed paraffin embedded question

Just got to thinking...I do animal tissue processing. I do use an automated processor, but it is open with no pressure or vacuum. If you think this will help I can give you the times I use. It is an overnight process and takes more than a work day worth of hours. Let me know? Sarah Goebel,

RE: [Histonet] Formalin-fixed paraffin embedded question

Are you volunteering? That is so nice of you =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Tissue Processors

Pathcenters in my opinion don't last as long? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Wage question for Wisconsin

At least you have a union!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On

RE: [Histonet] Section position on slides

I learned from an old school HT and studied using the Carson book. In this book there are several pages on where to put sections. If you don't have the Frieda Carson book (or the bible as I refer to it), get it. A new edition just came out about a year ago. If you're an ASCP member I think the

RE: [Histonet] cell block fixation

You could also try to make them with plasma and thrombin...Unless you are trying to do IHC with them, it will work fine. The IHC might become a problem because of the plasma donor's antigens might get in your way? After you make the booger just fix and it should be fine. Another way I have tried

[Histonet] Chloroform

Does anyone know of a special or IHC stain to detect chloroform? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list

[Histonet] Caspase 3

I know I have asked about this in the past, but I'm finally getting around to optimizing my antibody (Caspase3). First, tonsil will be a good control? Second, I ordered it from Abcam that is telling me the optimal dilution is between 1/10 and 1/20. This seems a little crazy? I looked at some

[Histonet] One more thing...I feel like Columbo

So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your

RE: [Histonet] microm parts for microtomes, only through Thermo?

I just ordered one from VWR. It's for a thermo tome which I think will fit on microms? It was $1600. I would call a tech help and see what they would suggest. Another thought would be to call IMEB (specifically Denise deVines 18005438496). I have had lots of good luck with the company and

RE: [Histonet] Paraffin

I have always liked paraplast. It's good because you don't seem to have as much tissue separation from the paraffin in the block. It also seems to hold up longer on the water bath to give sections a chance to de-wrinkle themselves without having to pull so much with forceps. It also doesn't

RE: [Histonet] (no subject)

Of course as most of us would say...histology rocks!! Most histotechs also do cytology is most hospital settings. The only difference is that cytotechs actually have to screen the slides that the histo people prepare. I think that cytologists make slightly more money...but histology is way more

[Histonet] Service

Hello all, I am in Austin Texas and would like to know who people use to service their equipment. I have used Biomed before and wasn't really impressed. Are there any other companies? The only thing I would really ever need service on would be the microtome (brand new Thermo 325). I'm just

[Histonet] Caspase

Look...I am a plethora of questions today!! Has anyone ever used the antibody Caspase? I hear lymph node is a good positive control, but where have people purchased the antibody, and at what dilution for FFPE tissue? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics

RE: [Histonet] Thyroid Smears

We always used 95% alcohol to fix, but you're right the blood usually comes off. Air dry then diff quik (or giemsa) stain works better for a hema-pathologic stain. You could try Pen Fix (or an alcoholic formalin) and this might help as opposed to the 95%. Why does the pathologist need to see

[Histonet] Cytology blender

Hey all, At an old job we had this tiny little blender with a kind of diaphragm top on it to blend sputum cytology specimens with. It probably only held about 200cc of fluid at the most. Does anyone have a clue where I can find one of these? Thanks Sarah Goebel, BA, HT(ASCP)

RE: [Histonet] Static issues

Breathing is my normal way to attack static, it's not a problem getting the ribbon...there is so much static that when I pull it off the tome, it literally sucks to my hand like a magnet...and then it's gone. Thanks everyone for your suggestions! I am hoping to only have to deal with this for a

RE: [Histonet] custom-made polyclonal antibody doesn't stain

I have done this (or tried to do this) as well and almost ended up bald from ripping out my hair!! My first suggestion would be to try to get down to a 1/25 or 1/50 dilution. If the background starts to get too high then try blocking the Fc region. You can buy commercially available Fc

RE: [Histonet] Tissue Processor Reagents

I think the standard rule of thumb is 700-800 blocks? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RRAS

Hello all!! Hope everyone is gearing up for the festivities of this weekend with great excitement!!! Got a quick question. We are going to be using the RRAS antibody and I'm trying to figure out what a good positive control tissue is. I looked on the abcam site and it says colon, spleen,

RE: [Histonet] Frozen Specimen Handling

Cutting frozen fat is virtually impossible. There are a few articles I have seen online (one is called something along the lines of the fat demon or something pun-tastic like that). Because fat is so oily it usually doesn't freeze at all. If you have a lot of fat in the tissue you are trying to

RE: [Histonet] Problems with histonet

I haven't received anything since Wednesday, but my email is blowing up now with Histonet stuff! I thought it was just my computer =) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent:

RE: [Histonet] sentinel nodes

Crystals maybe? How cold is the cryostat? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Re: Histology Consultant

Thanks Nathan!! I just don't want to be cheated on what I will be getting paid. My old job is wanting me to come in and do the small amount of histology work they have. This would all be on evenings and weekends. It is not PRN, more like call me once a month and I will come do the work. I'm

[Histonet] Processor change

How many blocks do you guys normally process before you change the solutions in the processor? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___

[Histonet] Histology Consultant

What is the going rate for a working histology consultant in Texas? For instance go in and process, embed, cut, and stain slides when needed? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext.

[Histonet] 70% alcohol

Hello all!! I moved jobs, but am still here in 80 degree Austin weather... Question. If tissue has been stored in 70% alcohol for up to a year, does there need to be anything done to it before processing? I am assuming that some rehydration might be necessary? If not can you just throw it on

[Histonet] Salary range

Hello all, Does anyone know what the salary range for a histology supervisor is in the Austin Texas area? Thanks Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907

RE: [Histonet] unstained paraffin tissue slides storage--why cold?

-70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay viable. I know over time they can degrade and so your stain won't work with some antibodies. The

RE: [Histonet] unstained paraffin tissue slides storage

Plain slide boxes is ok. I think you can store them for up to a year in a fridg. (4 degrees), but I usually pu them in a freezer (-20). Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907

RE: [Histonet] gram stain/label issues

Maybe try handwriting with a pencil then putting the label on aft erwards. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. = span style=color: rgb(51, 102, 255);8201 East Riverside Dr. Bldg 4 Sui= te 100 Austin, Texas 78744 (512)386-2907

RE: [Histonet] A Good Human Brain Control

American Matertech Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] A Good Human Brain Control From: Candice Smoots

RE: [Histonet] Need opinions on HE stainers please!

For the love of your sanity (and keeping your pathologists happy)= DO NOT BUY A SYMPHONY Their reagents are all proprietary and so troubleshooting is almost impossible without paying Ventana to have tech he= lp loom around your lab. The slides take 3 days or so to dry

RE: [Histonet] Cheap slide scanner for rat brains?

Do you have a dissecting scope you could use? You can grab one with a pretty low resolution camera for about $800. If you need more details let me know. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744

RE: [Histonet] Recommendations for decal solution

Best decal in the world...Formical-4. It has formalin in it so it fixes and decals at the same time. You can get plain Jane decal too. The company is called decal. Here is the website. I think you can also get it from Fisher? http://www.decal-bone.com/ Sarah Goebel, B.A., HT (ASCP)

[Histonet] Human anti human

Hey ya'll I was going through the archives to try and find a solution to this, but the last post was in 2008, so I thought I would throw it back out there. I need a human anti human polymer...is there one yet? I looked and couldn't find one through my normal places. As to not start the

RE: [Histonet] Un related to actual histology

Roll Roll Roll in the hay =) One of my all time favorite movies!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] Un related to actual

RE: [Histonet] Fwd: IHC coverglassing

Absolutely!! After the buffer rinse (after the counterstain), rinse in water, then 95, then 100, then xylene, and coverslip away!! I do it everyday and it works fine =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas

RE: [Histonet] Frozen sections

Chatter can also be caused by your blade angle. Try making the angle more obtuse, just don't over do it or you will get venitian (sp?) blind. The temperature is also a little cold. Try putting your thumb on the chuck, on top of the tissue, for a few sections before cutting and see if that helps

RE: [Histonet] Cassette Marking

I've used this one too, the tip is very tiny, but the pen is awesome! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: RE: [Histonet] Cassette Marking

RE: [Histonet] Plain Vanilla Autostainer?

What about just a simple old school ski stainer? Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Plain Vanilla Autostainer? From: Johnson,

RE: [Histonet] Cassette Marking

As compared to a lawsuit, yes it's cheaper. But, when trying to explain to a budget committee that you need something to label things that you can do by hand...they usually don't see the point. This brings me back to my original point of mislabelling things 2 times in a year... Sarah Goebel,

[Histonet] Eosin

Once upon a time I heard that eosin fluoresces is this true? = ; What color does it show up? Could this be used as a sort of back gr= ound stain so you can tell exactly how many cells are in the field? Sarah Goebel, B.A., HT (ASCP) Histotechnician = = XBiotech USA Inc.

RE: [Histonet] Un related to actual histology

I dressed up as Abby from CSI last year. Black wig, bright red lipstick, and a lab coat is all you need =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message

RE: [Histonet] Un related to actual histology

Actually it's NCIS... =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: RE: [Histonet] Un related to actual histology From: sgoe...@xbiotech.com

RE: [Histonet] Immunohistochemistry images

Are you wanting to save your specific images, or just need a refe rence guide for QC? Photobucket or Snapfish are two sites that are pr= etty cheap if not free? Sarah Goebel, B.A., HT (ASCP) Histotec= hnician XBiotech USA Inc. = /div 8201 East Riverside= Dr.

RE: [Histonet] negative controls

Pretty sure you have to run your positive with your negative to keep the conditions 100% the same. You don't have to put them on the same slide necessarily, but they need to be on the same run. Also, say you are running 15 HP patient slides, you can have one negative control for all of these as

RE: [Histonet] negative controls

Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the

RE: [Histonet] negative controls

So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in

RE: [Histonet] Defrosting Cryostat

With one cryostat it is a little bit difficult, but not impossibl= e. Turn it off at the end of the day (after all the possible frozen s urgeries are done) and let it defrost overnight. Have someone come in= an hour or so early to clean it out with 100% alcohol (or formalin

RE: [Histonet] Defrosting Cryostat

Even with a self-defrosting cryostat, if you are regulated by CAP= you still have to decontaminate it. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 E= ast Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 Original

[Histonet] Pathologists vs. Surgeons

Hey Ya'll, A friend of mine forwarded me this. We both are HTs and have had to deal with frozens a lot on a daily basis. Thought everyone might need a good chuckle on a Monday!! Cheers!! http://www.xtranormal.com/watch/6847761/ Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc.

RE: [Histonet] mislabeling of slides and blocks

Holy cow man!! Fired for mislabeling 2 times in 260 days!!= nbsp; Have you never been human and made a mistake?! I could understa= nd if it was a tech on a regular basis messing up, but that seems a little = crazy, especially if you are in a high volume hospital!!

RE: [Histonet] GLP Compliant Laboratory

What are you needing? Sarah Goebel, B.A., HT (ASCP) = Histotechnician XBiotech USA Inc. 8201 East R= iverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 Original Message Subject: [Histonet] GLP Compliant Laboratory From: Karie

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