RE: [Histonet] OT: Wednesday Half-Wit
We used to put a red bio-hazard bag (the heavy duty one's) into a cardboard box. This usually did not leak. Just saying if you still want to use your box method =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, July 06, 2011 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Wednesday Half-Wit I used to use Sub-X in the processor. Changing paraffin was no problem because I'd just drain it into a Styrofoam box, allow it to set, seal it up, mark it waste paraffin and put it into the regular trash. Easy, right? Well - then I changed back to using xylene. First time I changed paraffin, I used my tried-and-true method, only to arrive at the lab in the morning to find a partially dissolved Styrofoam box on the counter, surrounded by a solidified pool of paraffin. Eureka! Xylene dissolves Styrofoam! It was not pretty but I think I've invented a new space-age compound. Fortunately, I was struck by an Inspiration - use an empty xylene/alcohol bottle with the top cut off (so I can insert the paraffin drain). I don't know why I'm posting this except to show that when confronted with an unusual circumstance, women rule! Now, back to work. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vasculature in tumors
Hello all and happy long weekend (yes our boss gave us the rest of the day off at 2!!!) Looking to stain vasculature in tumors. I did trichrome, pentachrome, and PAS (with digestion) and am being told that these are not optimal for what they are looking for. Any other ideas to stain blood vessels would be awesome!! Thanks ya'll! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ...maybe a little more specific
So am getting lots of responses for IHC stains (which is ok), but I am doing these stains on mouse xenografts and the background is getting in my way a lot. Was just trying to find a simple special that would do the trick =) If IHC is the only way, so be it...was just hoping... What about Verhoeff??? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Luciferase
Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase
Someone just responded “only if it’s possessed” I laughed =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Angela Bitting [mailto:akbitt...@geisinger.edu] Sent: Thursday, June 30, 2011 11:13 AM To: histonet@lists.utsouthwestern.edu; Sarah Goebel; Rene J Buesa Subject: Re: [Histonet] Luciferase ok there is a really BAD religious joke coming soon, isn't there? Rene J Buesa rjbu...@yahoo.com 6/30/2011 12:00 PM You would have to have an antibody to it and I don't know any one exists, but I can be wrong. René J. --- On Thu, 6/30/11, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote: From: sgoe...@mirnarx.com sgoe...@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAS oops...
So I dumped my periodic acid like a moron...does anyone know of another reagent that will oxidize for the PAS reaction? Looks like I have acetic and hydrochloric acids...and that is all...hmm... Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Shriveling in Paraffin
You could have got the tissue wet or to hydrated. Sometimes after you face the block if you leave it on the ice to long and let moisture get into the tissue then cut the slide, it looks ok at the time, but once the moisture gets back out of the tissue it will start to look concave. If you are going to be leaving your blocks on the ice for a long time just put a 4X4 or paper towel under the block, this helps. Good luck =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle Aono Sent: Friday, June 24, 2011 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Shriveling in Paraffin I was cutting some bone/joint tissue and noticed that the cartilaginous portion was concave/indented, instead of flush with the rest of the block surface. Even as I continued to cut that portion always seemed a little sunken into the block face and all the sections crumbled. I didn't seal the block after I was done and when I came back the next day the entire tissue sample was shriveled and pulling away from the paraffin. I'm new, but in the few bone sections I've done I've never had this happen! Any ideas? ~Shelly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: HistoGel
You can also use agar. It does the same thing and is cheaper =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Milne, Katy Sent: Friday, June 24, 2011 12:27 PM To: 'histonet@lists.utsouthwestern.edu'; 'mjdess...@wvhcs.org' Subject: [Histonet] RE: HistoGel We use histogel a lot in our lab. It's a research lab and we use it for a few purposes - pelleting cultured cells then creating multi-culture TMAs for testing antibodies and also pelleting cells from ascites and pleural effusions. Has also been used to process really small samples that could have been lost in the processor through the cassettes. Works quite well. The researchers just put the samples in histogel and give it to me in formalin then I process it as I would regular tissue. Cuts very well too. Katy Message: 3 Date: Fri, 24 Jun 2011 12:26:46 -0400 From: Dessoye, Michael J mjdess...@wvhcs.org Subject: [Histonet] HistoGel To: histonet@lists.utsouthwestern.edu Message-ID: e2547e1cd0ee324488a2940994571efa0401f...@wvhcs-exchange.wvhcs.com Content-Type: text/plain; charset=iso-8859-1 Hello, Does anyone out there have any experience with HistoGel? It's Richard Allan/Thermo Fisher. They claim that you can embed scant tissues in the gel and then process, embed, and cut as usual. Just wondering how it works in the real world Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] bluing
Just add a little bit more ammonia hydroxide to the water =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, June 24, 2011 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my bluing step in the HE process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re: [Histonet] How many tissues an histo tech is suppose to cutper
Another solution...get out of clinical and go into research =) There are no quotas or slide per second expectations in the research world =) So sorry you are having such a bad time with your job. HT's are not a dime a dozen and usually it is fairly easy to find a better job. Not to mention research pays almost double the clinical world!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 26, 2011 9:43 AM To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Fw: Re: [Histonet] How many tissues an histo tech is suppose to cutper --- Joanne: Read the attachment so you can have an idea about productivity ranges and averages in different tasks in the lab. If you embedded 214 blocks and cut 148 in 5.5 hours, using the averages I provided, embedding should have taken 3.5 hours and cutting 6.2 hours which means you worked 1.76 times FASTER than the expected average. René J. --- On Sat, 6/25/11, Joanne joanne0...@comcast.net wrote: From: Joanne joanne0...@comcast.net Subject: [Histonet] How many tissues an histo tech is suppose to cut per To: histonet@lists.utsouthwestern.edu Date: Saturday, June 25, 2011, 7:07 PM i am quite serious in my presentation and request for advice. i too thought this goal was/is ridiculous to expect/ask for from someone so new and to attain in 6 months or less. last monday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for someone so very new i thought this pretty good . . . please note: most days aren't as hectic. :) what is an average though for blocks/minute? what is meant by set sum per block? ---keeping in mind i am new to this field. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
I second this motion!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, June 27, 2011 12:10 PM To: 'Joanne'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Austin PRN
Hello all =) Since I just recently got married and still own a house we do not live in, I am finding myself in the pickle of 2 houses and not enough income...Does anyone know of any kind of part time histology jobs in Austin that I could go to either early until around 7am or late after 5pm? Or if not histology specific, I could log specimens for intake or something...I just can't bring myself to ask the question...would you like fries with that? with a college education and 10 years of histology experience...I'm doing ok, I just want my fluff back =) Thanks ya'll!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Retirement
The smoking thing is what I missed...I know I work in cancer research and I shouldn't smoke, but as I tell everyone...by the time I get lung cancer I will have helped to find the cure (wishful thinking and stupid excuse making I know.) Now almost everywhere I work you have to completely leave the property to smoke, and the latest talk is that soon we won't be able to even smoke on a public sidewalk! I'm sure in my lifetime I will see cigarettes become illegal and pot become legal, kinda funny I think =) Happy Monday all!! PS-Is anyone else out there going to the Innovex thing in CA this week?? I need a happy hour/go to Alcatraz tour buddy =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Sunday, June 19, 2011 7:48 PM To: Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: Re: [Histonet] Retirement And remember mouth pipeting? oops, that's the cotton... Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello pat...@gmail.com wrote: Sheesh! We used to have people smoke while working with propylene oxide. Eating in your control pigs was part of the benefit of being a graduate student to save on grocery money. Film? My TEM used glass plates. Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. Wearing closed toed shoes was for wimps, you were just fast if you dropped a steel wedge blade. We even wrote using the entire word and proper grammar, none of this acronym stuff for us. Retirement? What's that? Paula :-) On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks amosbro...@gmail.com wrote: Agarose Gels! ... Listen you whipersnapper Agarose is the easy way out. When I learned it we used to have to make up our own polyacrylamide gels. That was after having to walk to work up hill both ways in 30 feet of snow! (No nearer retirement) Crotchety Amos Message: 7 Date: Fri, 17 Jun 2011 13:24:12 -0400 From: Emily Sours talulahg...@gmail.com Subject: Re: [Histonet] Retirement To: histonet@lists.utsouthwestern.edu Message-ID: banlktinejxtxyop-byfweuxn3yw-ff3...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Retirement? I think by the time I get to that point, social security will have run out. Then again, technology will be so advanced, I can tell stories about the old days, where I logged on to the bbs by modem to post messages to my friends and typed in my own html coding. We didn't have google when I was young!! Our cameras used film! And you couldn't see how bad your pictures were until you developed that film!! There was no PCR to sequence your DNA, you ran an agarose gel and hoped for the best!! You could drink the 100% ethanol, there was no denaturing! (okay that was before my time) You could smoke in the lab while you sectioned without gloves!! (okay that was too) Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAS
Quick question...if I am doing PAS (w/o digestion) and not looking for fungus...would kidney be an appropriate control?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Retirement
2035 huh?? Crap!! I won't be retired by then I'll be 54! Hmm...guess I need to invent the histo. robot so I can still have income!! =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 17, 2011 1:33 PM To: Cynthia Pyse; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retirement I have recently traveled back from the future, and it does not bode well for histologists. The robots are pretty reliable and can operate 14 hours on a single charge. They also do not have to wear safety glasses when working with the laser microtomes. No vacations, no calling in sick, no travelling to seminars (new software version upgrades come to them) I noticed that someone still has to come in in the morning to flip the switches, but since CLIA will not likely consider that testing Well, who'll need a histologist for that? Funny though, pathologists are still around, but they are all workiing in a single building near Area 72. (you don't want to know about area 72 unless you are still around in 2035, then EVERYONE will know about area 72) They spend their full 6 hour day looking at scanned images on big-screen monitors and drinking coffee. It's not that the future really needs them, but the AMA still lobbies for them more effectively than the ASCP for us. - Bill With apologies to all the really decent pathologists who monitor this forum. (I have to be nice because my only usable skill someday might be making a mean cup of coffee) -Original Message- From: Cynthia Pyse [mailto:cp...@x-celllab.com] Sent: Friday, June 17, 2011 12:51 PM To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retirement As long as we don't have to go back to steel knives, old AO micrtomes and the autotechnicon I'm in. I am also 15 to 18 years out. What is the world of histology going to do without us. Who will know how to make a solution of mucicarmine (not that I do anymore, but I could) or eosin? The tech I train now look at me like I am speaking a foreign language sometimes. Make me feel old, but closer to retirement. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 17, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retirement OK, I know it is Friday, and I know that this may sound like a bit of a jokebut I am 15-18 years out from retirement and my wife and I want to retire someplace tropical And it would be smart to get settled in such a location. So, if anyone knows of any openings in Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT (ASCP) QIHC PLEASE let me know. Would be open to others, but would prefer a US territory. I can be reached at b...@deaconbill.com William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Keeping Histo room floor clean?
You can buy this type of thing too if you aren't the McGiver type. For instance...from American Mastertech item # CPW04200E Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Tuesday, June 14, 2011 7:50 PM To: JR R Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Keeping Histo room floor clean? We don't keep it off the floor, but do use a wide-bladed putty knife attached to a mop handle to scrape the residual wax off the floor. It woks quite nicely and doesn't remove the actual floor wax like a razor blade scrapper would. Michelle Sent from my iPhone On Jun 14, 2011, at 6:44 PM, JR R rosenfeld...@hotmail.com wrote: We keep getting a lot of paraffin on the floor of one histo room--especially around the microtome and the embedding station. Short of laying down a tarp, what do folks do keep wax off of the floor? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Retirement
I'm 30, so blah blah blah...I have 30 or 40 more YEARS!! =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, June 14, 2011 2:26 PM To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Retirement 7 years compared to 8 months is gigantically significant. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, June 14, 2011 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retirement Some of my compatriots are laboring under the assumption that retirement is sooner than later. If it were only true... my planned Date of Departure (pending an Offer I Cannot Refuse) is 29 February 2012. I have many ideas for interrupting your days until then... Fear not! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vimentin
Does anyone have a picture of a positive control Vimentin on human tonsil? I'm getting some arguments... Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide Bright Question
Ahhh!!! I had this same problem when I came to this lab too!! Plus if you put that xylene substitute crap (mine was clear rite) it screws up cell blocks that have been embedded into agar!! 5 experiments had to be repeated because of this stuff!! The eosin bleed is because you got water on the slides after the eosin. With xylene you can see the water pool at the bottom, but with these substitutes it just kind of becomes part of the solution. I have finally gone back to xylene! We had to buy hoods, but it's the cost of better histology =) Fixing the eosin bleed...uncoverslip, run through xylene (or let sit in the substitute for 15 or 20 minutes, then alcohols, then in running water for 10 minutes or so. Then change all your alcohols to fresh solutions (I would change the substitutes too), then from water to alcohol, eosin, etc. etc. and they should be fine. Good luck!! I wanted to pull my hair out a couple months ago because of that stuff...run away from it!!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher Conlisk Sent: Friday, June 03, 2011 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Bright Question Hello Everyone, I have worked in labs that use Xylene my entire career and I just started at a Lab That only uses a Xylene Substitute Slide-Bright. I am having problems with the HE. After staining and coverslipping (The slides look fine innitially), Then about 5-10 minutes after coverslipping the Eosin starts bleeding out all around the tissue. I have asked several of my Histotech Friends that are old timers and they say that Xylene Substitutes are awful at deparrifinization and awful at clearing. They told me that the alcohol isnt getting thoroughly cleared in the Slide Brite and then it is eventually leeching out after coverslipping??? Is this true and does anyone have any guidance for this issue? We also run MOHS slides on the same stainer and I keep all the reagents clean as a whistle. I really hate Xylene Substitute's Thanks C.S. Conlisk HT(ASCP), PBT(ASCP) Kansas City Skin and Cancer Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slides for IHC
So I just cut a bunch of slides for Ki-7...I am not going to be able to stain them until Monday. I know the best thing is to put them in 4 degrees, but will it hurt them to stay at room temp for 3 days? Putting them in the fridge gets a lot of moisture under them and they tend to lift more. Any other suggestions...the tissue is getting low in the block, and I don't want to recut them Monday. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tumors tumors everywhere
So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Two Questions
Usually I think it has to have a door for fire purposes. Especially since there are so many flammable chemicals in the lab. I could be wrong? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Wednesday, April 27, 2011 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two Questions 1st. Does anyone know if there is a rule or law that states a lab must have a door? 2nd Does anyone know how a TC only lab would do profiency testing on HE slides. They do not have physician on staff and no microscope. Does the company that is reading PC lab do profiency testing on the QC slides and share results with the TC side? Any thoughts would be appreciated. Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problem with IMEB
I have had excellent luck and service with them. Call Denise deVines she is super nice and helpful =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 27, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem with IMEB Hi all. Has anyone had difficulties in purchasing refurbished equipment from IMEB? I have been trying to purchase a T2000 ThinPrep processor and sent 50% payment with net due in 30 days. This transaction started about a month ago and they've had our partial payment for more than 3 weeks but had not shipped the instrument. I've repeatedly called to get the status of shipment but have gotten the run around with checking our credit references. This transaction has left a very bad taste in our mouths so I proceeded to request our payment back and cancel the order yesterday. Now, IMEB says they'll return our deposit minus a 20% restocking fee for an item that never even left their facility. I was surprised to hear of this from IMEB given I'd heard they were a reputable company. My opinion has definetly changed! Just wondered if anyone else has had a similar experience with them??? Thanks for lending an ear (or many). Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Positive control
Does anyone know what the positive control for MET is? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] endogenous peroxidases
So I have been seeing from this post that people block prior to HIER? I have always done it as the first step after for 10 minutes (I use the DAKO enzyme blocker). Is this wrong? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, April 22, 2011 12:42 PM To: Hobbs, Carl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] endogenous peroxidases Make sure you use a fairly fresh bottle of H2O2. (no older than 6 months). The stock bottle degrades after it is opened. I have seen people using an older bottle of H2O2 and the results are sub-optimal. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote: Gimme 5! Block before or after Ag retrieval. If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2 in Dist.water. Block for 15mins. Methanol is not neccessary...imho. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus London SE1 1UL Tel: 020 78486813 Fax: 020 78486816 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] eosin bleeding on frozen sections
This happened to me a couple weeks ago. You got water on the slide after the eosin staining. Just run it back through alcohols, and rinse for about five minutes. Change out the alcohols, and re-stain. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christi Cosby Sent: Thursday, April 21, 2011 11:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin bleeding on frozen sections Hi, In our lab we are having trouble with the eosin bleeding off the section after coverslipping. We are performing a rapid HE stain on frozen sections. Any advice would be greatly appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] decalcification of articular cartilage
You could always get formical which fixes and decals at the same time. This would eliminate the waiting for fixation. Although I do usually let it fix in straight formalin overnight first. Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, April 21, 2011 12:59 PM To: Nejat Yilmaz; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decalcification of articular cartilage We do a lot of IHC staining on bone and cartilage samples and we use 10% formic acid for decalcification for immunohistochemistry. I'm afraid you may not be able to turnaround this in a week. The tissue needs to be adequately fixed prior to decalcification, and properly decaled. I would trim the tissue to about 4 mm thick if you can on a saw you might be able to turn this around in your time frame, but I have learned from experience if you rush decalcification you have a greater chance of running into problems. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz Sent: Wednesday, April 20, 2011 8:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcification of articular cartilage Dear Members, We're planning to study sheep knee articular cartilage with histochemistry and immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde at the moment. We need an effective, quick and cartilage tissue protective decalcification method. We'll perform only light microscopic examination in this study and we have to complete the study within one week. In these circumstances what are your advices? Thanks in advance. Dr. Necat Yilmaz University of Mersin School of Medicine __ ESET NOD32 Antivirus Akıllı Güvenlik tarafından sağlanan bilgiler, virüs imza veritabanı sürümü: 6057 (20110420) __ İleti ESET NOD32 Antivirus Akıllı Güvenlik tarafından denetlendi. http://www.nod32.com.tr ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thank you all!!
So...I sacrificed a couple of the slides and stained with HE per ya'lls suggestion and...yay!! Cells all over the place. See I freaked myself out for nothing. Now, where is 5:00? =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] uh...
So I just stained a group of slides all at the same time with the same conditions. About 40 of 150 the eosin looks like it is bleeding out of the sections...this has never happened before? What could be the cause and how do I fix it? Everything is as normal, but again I am being forced to use the crappy stuff called clear rite. Could this be the cause of the bleeding? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] National Average Salary
So apparently I'm underpaid =) Good Luck convincing employers of this! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, March 31, 2011 9:35 AM To: Lin Bustamante Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] National Average Salary Salary survey is in the March 2011 issue of LabMedicine from ASCP. You can also get it from www.ascp.org web site. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante Sent: Thursday, March 31, 2011 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] National Average Salary Can anyone please send me the National Average Salary chart for Histotechnician and HT Supervisor with more than 5 years experience? Thank you very much. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April 1st Fun
The funny part is I hear that can be a fungus control!! =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Tuesday, March 29, 2011 2:53 PM To: Breeden, Sara Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] April 1st Fun We had a patient we named Oscar Meyer... it was a piece of hot dog that we put in formalin, the doc grossed it, we cut it (and it cut very well, I might add!) and they got to the point of looking under the scope before they realized the joke! ;o) Michelle On Mar 29, 2011, at 2:13 PM, Breeden, Sara sbree...@nmda.nmsu.edu wrote: Dead cockroach on autopsy/necropsy table as legitimate specimen; goldfish processed as patient Barry Cuda; change family photos to total strangers; butterscotch pudding in (unused) specimen jar and subsequent tasting with applicator stick; activated charcoal on oculars (a la Colonel Potter in MASH); single hair attached to ocular with super-glue (tickling nose); cut fingertips off gloves; pumpkin/squash in animal shapes to slide; bug in block; foam-rubber-stuffed-box-with-toy-rat-to-burst-out-and-cause-startlement (toy rat to morph into histo lab mascot complete with appropriate holiday costume); process beef jerky as legitimate specimen; light coating of oil on 'scope oculars; - and the latest one I just received - a small slip of paper on the underside of the (computer's) mouse. Oh - the joy! What shall I do first?! Happy Annoying! This ought to cover the last several months I've not done the Friday Hour of Fuming... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stupid Rabbit primaries!
So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Stupid Rabbit primaries!
I blocked for all of those... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Tuesday, March 22, 2011 4:13 PM To: Sarah Goebel; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Stupid Rabbit primaries! Do you know for sure that its the Ab and not endogenous biotin, peroxidase or Phosphatase? You can block for all those. Otherwise there are a number of commercial blocking agents on the market...try Biocare for a start (800)799-9499 or better yet, the company that makes your antibody. Good luck. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Tuesday, March 22, 2011 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Storing slides in buffer
I just did this last week. I stored them in wash buffer overnight in the frig. and they were fine =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stacy Deppeler Sent: Wednesday, March 16, 2011 3:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storing slides in buffer Hi guys, I am wondering if anyone has much experience storing slides in buffer (PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they will still stain? Or is it going to be entirely dependent on the epitope? I'm optimizing research antibodies right now and I'd like to decrease my turn around time by knocking off the HIER at an earlier time point. Thanks. -- Stacy Deppeler. Research Assistant Department of Ophthalmology King Abdulaziz University Hospital Riyadh, KSA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Polymer detection kit
I use the Biocare polymers all the time because they are cheaper than DAKO. Some of them are biotin free, so as long as your primary has no biotin and you aren't staining anything with endogenous biotin you can omit that block. Other than that enzyme and protein blocking I still do. Some of them are a two step process and some are one solution. They work great! Just follow the protocol included with the polymer =) Good Luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of amitapan...@torrentpharma.com Sent: Friday, March 11, 2011 4:54 AM To: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: [Histonet] Polymer detection kit Dear Histonet fellow, I am planning to use Bio care detection kit -MRT511 (for mouse monoclonal antibody on rat kidney FFPE) and RMR622 (for rabbit polyclonal antibody on rat kidneyFFPE) Do i have to buy any additional reagents to perform the staining.(specially blocker or antigen retrieval) Any feed back on their results and trouble shootings are welcome. Amita ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cell blocks
I actually fixed the cells without the agar overnight, and left them in the agar overnight... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: Tony Henwood [mailto:antho...@chw.edu.au] Sent: Tuesday, March 08, 2011 6:43 PM To: Sarah Goebel; histonet@lists.utsouthwestern.edu Subject: RE: cell blocks Sarah, It is important that the agar cell block is allowed to fix in formalin (10%NBF) before wrapping in paper, at least 2 hours. It will hold its shape better. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Wednesday, 9 March 2011 2:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell blocks So...I am trying to make a cell block using 0.9% agar. I have done this in the past with no problems. It was at a different facility and I don't know if the agar is the same? I used bacto-agar this time, but last week someone tried to do my protocol with agarose and the boogers dried up and fell out of the block. The only other difference is we have clear rite xylene substitute in this processor where the other one I used had xylene. Tissues processed on the same cycle come out fine with the clear rite so I don't think that it the problem. When the cell blocks come out they are as thin as the papers I wrapped them in making it almost impossible to see and to subsequently cut. Any suggestions for making cell blocks without using the plasma/thrombin method or the insanely expensive histo-gel would be appreciated. Second, does anyone have a suggestion on how to repair the cells that are embedded in the tissue paper thick cell block? Thanks guys and gals! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] diastase
Just hack up some spit... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 09, 2011 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome safety
I use the end (without bristles) of a paintbrush Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cell blocks
So...I am trying to make a cell block using 0.9% agar. I have done this in the past with no problems. It was at a different facility and I don't know if the agar is the same? I used bacto-agar this time, but last week someone tried to do my protocol with agarose and the boogers dried up and fell out of the block. The only other difference is we have clear rite xylene substitute in this processor where the other one I used had xylene. Tissues processed on the same cycle come out fine with the clear rite so I don't think that it the problem. When the cell blocks come out they are as thin as the papers I wrapped them in making it almost impossible to see and to subsequently cut. Any suggestions for making cell blocks without using the plasma/thrombin method or the insanely expensive histo-gel would be appreciated. Second, does anyone have a suggestion on how to repair the cells that are embedded in the tissue paper thick cell block? Thanks guys and gals! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fat tissue
For the most part you cannot section fat on a cryostat because of the oilyness (is that a word). You can fix it and section it? Good Luck Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaret Blount Sent: Wednesday, March 02, 2011 4:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fat tissue Has anyone experience of cryostat sectioning of fat tissue? This would be rodent fat pads, particularly the gonadal fat pad which usually consists largely of adipocytes. What temperature would you cut at? Is there anything that could be used to infiltrate the tissue prior to sectioning to provide more support? Regards Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC slides
So...I have run down slides and done antigen retrieval on my FFPE slides. They are currently in antigen retrieval that has come to room temperature. I am not going to be able to finish the IHC stains until tomorrow. Will it be better to keep the slides in the antigen retrieval solution at 4 degrees overnight, or put it in buffer at 4 degrees overnight? I really don't want to stay here late. Thanks histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Telepathology
It's not nice to make fun of people's typing boo-boo's, but thanks for the Friday chuckle!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, February 25, 2011 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Telepathology Okay, it's Friday so I have to write that my first thought was telepathology? Is that like telekinesis? Like processing slides and coverslipping with your mind? Would this extend to sectioning to? How about pouring your much needed cup of coffee, or even making the coffee? Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Fri, Feb 25, 2011 at 10:24 AM, Andrew Byrnes a.byr...@accelpath.comwrote: Dear Histonet, Thank you for your responses to my email this past week! If you have any additional thoughts about telepathology or digital pathology, please email me or call me directly. Have a nice weekend! Andrew Andrew Byrnes VP Sales and Marketing AccelPath, LLC M: 732-312-8008 www.AccelPath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Polymer detection kit on rat tissue
I personally love polymers!! The DAKO envision is actually using polymers. You don't have to buy the kit. Biocare has a ton of awesome polymers for sale that I use in tangent with DAKO. If you want more specifics on my protocols let me know. Good Luck Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of amitapan...@torrentpharma.com Sent: Thursday, February 24, 2011 4:12 AM To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: [Histonet] Polymer detection kit on rat tissue Dear histonetters, I am lost in data sheets of IHC detection kits . Please help me to finalize one good detection kit for my purpose. I am planning to do immunohistochemistry on paraffin embedded rat kidney tissues for mouse monoclonal and rabbit polyclonal antibody (not double labelling). I am looking on Dako envision, vector immpress, Novolink , the company like Biocare/ Max bio vision suggest the Rat specific detection kitGot confused... Also send me your opinion on ABC detection versus polymer detection system. Thanks in advance for your view. Amita ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cdj
Ahhh!! Everytime I hear those letters I have flash backs to my hospital days!! Stupid mad cow disease!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Thursday, February 24, 2011 4:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cdj Hello everyone We recently recieved a case possible positive for CJD. In researching this we have found that they now say Formalin is BAD. As long as I have been a Histo tech it seems the rules were Formalin then Formic acid. But seems there are some studies saying this is no longer enough. Is there anyone out there that has changed and if so What are you doing now??? Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP
What?!? A histology lab with no enough space...who ever heard of such a thing Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, February 23, 2011 9:29 AM To: histonet; Akemi Allison Subject: Re: [Histonet] CAP We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. Akemi Allison akemiat3...@yahoo.com 2/23/2011 9:02 AM Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RRAS
So...trying to find someone who sells RRAS antibody that isn't Abcam. I can't seem to make the Abcam antibody work and I have been asked to try and get it from a different company. 2. Does anyone have any experience with RRAS? I have tried dilutions from 1:500 all the way up to 1:2000. I keep getting a blush endogenous stain that I can't seem to get out even after blocking for everything under the sun. I am using a normal pH antigen retrieval (DAKO). Should I try high pH? I don't know if that will make the positive staining come out, I think it will make it worse? Thanks histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin-fixed paraffin embedded question
Just got to thinking...I do animal tissue processing. I do use an automated processor, but it is open with no pressure or vacuum. If you think this will help I can give you the times I use. It is an overnight process and takes more than a work day worth of hours. Let me know? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaret Blount Sent: Tuesday, February 22, 2011 3:02 AM To: Noel Gray; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin-fixed paraffin embedded question I agree with all the comments so far, and I think all the steps are far too long, particularly the overnight in molten wax. You are effectively cooking your samples. I would avoid overnight in 100% ethanol too; it will tend to harden the tissue. Get hold of the manual for animal tissues available from the Society for histotechnology. I found this invaluable and now most of my tissues can be sectioned without soaking at all. I just chill them slightly, if necessary. Good luck with your samples. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Noel Gray Sent: 21 February 2011 20:55 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin-fixed paraffin embedded question I am having an issue with formalin-fixed, paraffin embedded tissue that I am sectioning. I am using a microtome to cut the tissue (mouse brain, cerebellum and spinal cord) in 10 um sections which I will stain using cressyl violet. Sometimes, the tissue in a block will splinter once it hits the blade. Usually all samples from the same animal splinter but this is not always the case. If I put the block into the water bath (sectioning surface exposed to water or not) this seems to stop the splintering for 1-200 um worth of tissue. However, I am afraid this may bring error into the histological analysis of my tissue. I assume it has something to do with the protocol I am using to prepare the tissue. I guessed that maybe the dehydration, alcohol clearing, or paraffin infiltration are not complete, resulting in the problem I have. However, I looked at various FFPE protocols and each of my wash steps are longer, which may be the problem? I was wondering if anyone has encountered this before, or if anyone knows exactly what is going on with my tissue and how I can fix it? Thank you. Here is my protocol: -Anesthetize mouse followed by a system flush of 30 ml of PBS and then slow perfusion of 50 ml of 4% PFA. -Brain and spinal cord are removed as a single, in tact unit and placed into 70% ethanol for 4 hours -80% EtOH for 4 hours -90% EtOH over night -100% EtOH #1 for 4 hours -100% EtOH #2 for 4 hours -100% EtOH #3 over night, forebrain cerebellum and spinal cord are separated -Xylene wash #1 for 4 hours -Xylene wash #2 for 4 hours -Xylene wash #3 over night -Moulton paraffin wash #1 for 4 hours -Moulton paraffin wash #2 for 4 hours -Moulton paraffin wash #3 over night -Tissue is embedded and then sectioned into 10 um sections Again thank you for your time. Noel W Gray Neuroscience Graduate Program SUNY Upstate Medical University 3219 Weiskotten Hall 766 Irving Ave Syracuse, NY 13210-1630 (315) 464-8144 gr...@upstate.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin-fixed paraffin embedded question
Are you volunteering? That is so nice of you =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Tuesday, February 22, 2011 9:48 AM To: Margaret Blount Cc: Noel Gray; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin-fixed paraffin embedded question The NSH Manual needs to be seriously updated. There is no protocol for mouse brain that could help this guy with his problem. Andi On Feb 22, 2011, at 2:02 AM, Margaret Blount wrote: I agree with all the comments so far, and I think all the steps are far too long, particularly the overnight in molten wax. You are effectively cooking your samples. I would avoid overnight in 100% ethanol too; it will tend to harden the tissue. Get hold of the manual for animal tissues available from the Society for histotechnology. I found this invaluable and now most of my tissues can be sectioned without soaking at all. I just chill them slightly, if necessary. Good luck with your samples. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Noel Gray Sent: 21 February 2011 20:55 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin-fixed paraffin embedded question I am having an issue with formalin-fixed, paraffin embedded tissue that I am sectioning. I am using a microtome to cut the tissue (mouse brain, cerebellum and spinal cord) in 10 um sections which I will stain using cressyl violet. Sometimes, the tissue in a block will splinter once it hits the blade. Usually all samples from the same animal splinter but this is not always the case. If I put the block into the water bath (sectioning surface exposed to water or not) this seems to stop the splintering for 1-200 um worth of tissue. However, I am afraid this may bring error into the histological analysis of my tissue. I assume it has something to do with the protocol I am using to prepare the tissue. I guessed that maybe the dehydration, alcohol clearing, or paraffin infiltration are not complete, resulting in the problem I have. However, I looked at various FFPE protocols and each of my wash steps are longer, which may be the problem? I was wondering if anyone has encountered this before, or if anyone knows exactly what is going on with my tissue and how I can fix it? Thank you. Here is my protocol: -Anesthetize mouse followed by a system flush of 30 ml of PBS and then slow perfusion of 50 ml of 4% PFA. -Brain and spinal cord are removed as a single, in tact unit and placed into 70% ethanol for 4 hours -80% EtOH for 4 hours -90% EtOH over night -100% EtOH #1 for 4 hours -100% EtOH #2 for 4 hours -100% EtOH #3 over night, forebrain cerebellum and spinal cord are separated -Xylene wash #1 for 4 hours -Xylene wash #2 for 4 hours -Xylene wash #3 over night -Moulton paraffin wash #1 for 4 hours -Moulton paraffin wash #2 for 4 hours -Moulton paraffin wash #3 over night -Tissue is embedded and then sectioned into 10 um sections Again thank you for your time. Noel W Gray Neuroscience Graduate Program SUNY Upstate Medical University 3219 Weiskotten Hall 766 Irving Ave Syracuse, NY 13210-1630 (315) 464-8144 gr...@upstate.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processors
Pathcenters in my opinion don't last as long? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, February 17, 2011 7:56 PM To: Histonet Subject: [Histonet] Tissue Processors Greetings all, if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help Joe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Wage question for Wisconsin
At least you have a union!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, February 15, 2011 6:48 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Wage question for Wisconsin The answer to that question may have to wait a week or two. At least here at the UW. Our wonderful governor is trying to get rid of the state workers unions and collective bargaining. He is trying to push it through (it was just introduced this last Friday), and the vote is slated for later this week. The techs at the UW hospital have fortunately signed their contract that is good until the reopener in 1013 and we are not strictly state employees. However, the way things are looking, it is not out of the question that he may also try to void our contracts. Our pay MAY go up, but we will no longer have much, if anything, for benefits. Don't count on sending your kids to the UW colleges if this goes through. Sorry, I'm fighting mad right now. I'm not usually big on the unions, but this is HUGE for millions of Wisconsinites. GGR! Claire Were there any other Wisconsin techs at the rally today? Even the Police and Firefighters unions were down there! From: histonet-boun...@lists.utsouthwestern.edu on behalf of Michael Hillmer Sent: Tue 2/15/2011 5:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wage question for Wisconsin We are dermatology clinic in Northeast Wisconsin and we are trying to do gather accurate wage data. Can anybody offer wages for an HT, HtL and lab assistants? Any help would be greatly appreciated. Thank you- Michael Hillmer PHR HR Coordinator Dermatology Associates of Wisconsin Phone: (920)683-5278 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Section position on slides
I learned from an old school HT and studied using the Carson book. In this book there are several pages on where to put sections. If you don't have the Frieda Carson book (or the bible as I refer to it), get it. A new edition just came out about a year ago. If you're an ASCP member I think the price is reduced... For the most part, just put everything as close to the middle of the clear glass part of the slide as possible. This also does well for IHC automation. I think most automated machines you can adjust the drop range too if you want to put the sections on the bottom or top? Good Luck! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Tuesday, February 15, 2011 11:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Section position on slides I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications? 1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. x mm from the edge of the glass slide)? 2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable? Thanks for any ideas. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cell block fixation
You could also try to make them with plasma and thrombin...Unless you are trying to do IHC with them, it will work fine. The IHC might become a problem because of the plasma donor's antigens might get in your way? After you make the booger just fix and it should be fine. Another way I have tried is to used an agar solution to make the pellet. Good Luck! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, February 14, 2011 9:31 AM To: Hutton, Allison; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cell block fixation Try a 50/50 mix of formalin and 95% alcohol. Have your prep techs add about 5 mL of this mixture and a drop of albumin (we use the bovine albumin from the blood bank but any albumin will do) to the cell block contents. Mix well and centrifuge. The button should be well formed. Take care not to add too much albumin or the tissue will be brittle and difficult to cut. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Wednesday, February 09, 2011 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block fixation We recently switched vendors for our formalin and while we have not experienced any difference with our surgical specimens, our cell blocks from body fluids have been giving us a great deal of trouble. The button that we get never seems to harden, leaving it sort of gelatinous, even if left to sit in formalin for days. We are able to get sections off of these cell blocks, however, the slides are blank by the end of the staining process. This is only a recent development that seems to coincide with the time we switched formalin vendors and it only happens with body fluid specimens (FNA specimens don't seem to give us as much trouble). The composition of the formalin is almost identical between vendors. Can anyone help me explain why this might be happening? Thank you in advance, Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chloroform
Does anyone know of a special or IHC stain to detect chloroform? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Caspase 3
I know I have asked about this in the past, but I'm finally getting around to optimizing my antibody (Caspase3). First, tonsil will be a good control? Second, I ordered it from Abcam that is telling me the optimal dilution is between 1/10 and 1/20. This seems a little crazy? I looked at some reviews for the antibody and other people seem to have used this dilution...wow! This is going to be an expensive antibody to use!!! Anyhoo, main thing is want to make sure that tonsil will be ok for a positive control =) Stay warm my fellow Texans...it'll be 80 on Sunday!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] One more thing...I feel like Columbo
So back at an old job we had an embedding station that had forceps that plugged in and were constantly hot. Does anyone know where I can just get the forceps that are always hot? I have the wells in my embedding center, but it gets frustrating when embedding multiple tiny mouse tissues and your forceps get cold and have to switch them and now your paraffin is getting cold... Let me know if anyone knows where I can get a plug in heated forcep =) Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microm parts for microtomes, only through Thermo?
I just ordered one from VWR. It's for a thermo tome which I think will fit on microms? It was $1600. I would call a tech help and see what they would suggest. Another thought would be to call IMEB (specifically Denise deVines 18005438496). I have had lots of good luck with the company and Denise is great! They do a lot of refurbishing of equipment and maybe she could help you out or at least point you in the right direction. Ok Denise...I get a cookie with my next order right =) Note: I am in no way affiliated with IMEB or getting anything for this suggestion, I just think it's a great company and wanted to pass it along to everyone!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Wednesday, February 09, 2011 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microm parts for microtomes, only through Thermo? Hi All, I have a blade assembly that is giving grief. I would like to just adjust the set screws to fix it and will try. In checking for a new assembly I got a price of 2220 for just the blade holder. Are there any companies out there that manufacture after market products like this? Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin
I have always liked paraplast. It's good because you don't seem to have as much tissue separation from the paraffin in the block. It also seems to hold up longer on the water bath to give sections a chance to de-wrinkle themselves without having to pull so much with forceps. It also doesn't seem to be as oily as some other paraffins. Just my two cents =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Thursday, January 27, 2011 1:07 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Paraffin HI all Just wanted to get peoples opinion's on the paraffin you use for processing animal tissues. I am interested in why you use a particular type, pros and cons. This query would apply to mouse and rat but I would also be interested in other species specific information if you have. You do not have to provide vendor info and feel free to email me off the net. If people are interested, I can compile and resend to the histonet Thanks L Luis Chiriboga Experimental Pathology Core Laboratory New York University School of Medicine /PRE html body br / This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.br / = /body /html PRE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Of course as most of us would say...histology rocks!! Most histotechs also do cytology is most hospital settings. The only difference is that cytotechs actually have to screen the slides that the histo people prepare. I think that cytologists make slightly more money...but histology is way more fun!! I would say maybe try and go visit a lab that has both types of techs there and see what looks like it would fit you best. Good luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 26, 2011 1:47 PM To: Histonet Subject: [Histonet] (no subject) Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Service
Hello all, I am in Austin Texas and would like to know who people use to service their equipment. I have used Biomed before and wasn't really impressed. Are there any other companies? The only thing I would really ever need service on would be the microtome (brand new Thermo 325). I'm just trying to see whether a maintenance contract would be more cost effective to pay yearly than to get someone out if something goes wrong with the tome. Since it's just a plain jane rotary microtome, usually things don't really ever go wrong so I think I know the answer, but nonetheless would like repair guy/gal information. Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Caspase
Look...I am a plethora of questions today!! Has anyone ever used the antibody Caspase? I hear lymph node is a good positive control, but where have people purchased the antibody, and at what dilution for FFPE tissue? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Thyroid Smears
We always used 95% alcohol to fix, but you're right the blood usually comes off. Air dry then diff quik (or giemsa) stain works better for a hema-pathologic stain. You could try Pen Fix (or an alcoholic formalin) and this might help as opposed to the 95%. Why does the pathologist need to see blood cells in a thyroid asp.? Just my 2 cents =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, January 12, 2011 8:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thyroid Smears Hello Everyone! Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions. Thank you in advance, Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytology blender
Hey all, At an old job we had this tiny little blender with a kind of diaphragm top on it to blend sputum cytology specimens with. It probably only held about 200cc of fluid at the most. Does anyone have a clue where I can find one of these? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Static issues
Breathing is my normal way to attack static, it's not a problem getting the ribbon...there is so much static that when I pull it off the tome, it literally sucks to my hand like a magnet...and then it's gone. Thanks everyone for your suggestions! I am hoping to only have to deal with this for a short while longer. I like the penguin idea just cause I think that will be the funniest!! Thanks again!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, January 06, 2011 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Static issues This is New Mexico where humidity is a rumor. The humidity in the lab here - as I write - is 18% and that's on a really wet day! If I have static issues with my ribbons, I just lean a little bit toward the block and breathe on it and the ribbons just float (in a good way) off the knife. I do that so often that when I use my sewing machine, I find myself breathing on the material. That's just sad! But try the Breathing Thing. Or not. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] custom-made polyclonal antibody doesn't stain
I have done this (or tried to do this) as well and almost ended up bald from ripping out my hair!! My first suggestion would be to try to get down to a 1/25 or 1/50 dilution. If the background starts to get too high then try blocking the Fc region. You can buy commercially available Fc blockers. I also tried conjugating the antibody with HRP which seemed to help some as well. Good Luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Verstraeten Sent: Tuesday, January 04, 2011 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] custom-made polyclonal antibody doesn't stain Dear all, I had two antibodies made (in rabbit) for the same peptide. It was the first time I did this : the firm designed the immunization peptide, injected and bleed the rabbits and purified the antibody. I have tested it on paraffin sections: all background, no staining where it should be. I tried different retriever buffers and detection with dab and fluorescence. I have tested it on western blot: a lot of background, dirty lanes. To get a clearer view I performed a co-IP. Still not really great. Can anybody help me on this issue? I just want it to work for staining. Thanks a lot in advance! Barbara Verstraeten, Drs. Evolutionary Developmental Biology Department of Biology Ghent University K.L. Ledeganckstraat 35 B-9000 Ghent Belgium tel: ++32/(0)9 264 52 31 fax: ++32/(0)9 264 53 44 http://www.evodevo.ugent.be ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Reagents
I think the standard rule of thumb is 700-800 blocks? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Katherine Leonard Sent: Tuesday, January 04, 2011 12:32 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Reagents Hello histonetters ~ Is there any consensus regarding how often to change processor reagents? I am using the TBS ATP1 tissue processor and the tissue size ranges from biopsy to 1.5cm^3. -- Katherine Leonard Research Associate ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RRAS
Hello all!! Hope everyone is gearing up for the festivities of this weekend with great excitement!!! Got a quick question. We are going to be using the RRAS antibody and I'm trying to figure out what a good positive control tissue is. I looked on the abcam site and it says colon, spleen, heart, CA lines. I don't know if it means colon cancer, etc. or if those three tissues and then any cancer line of any tissue would also work. I currently only have breast and prostate cancer tissues. I do also have normal heart, colon, and spleen. Does anyone have an answer for this? Thanks in advance, my fellow histo. hotties!!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Frozen Specimen Handling
Cutting frozen fat is virtually impossible. There are a few articles I have seen online (one is called something along the lines of the fat demon or something pun-tastic like that). Because fat is so oily it usually doesn't freeze at all. If you have a lot of fat in the tissue you are trying to freeze and not just a big hunk of fat (say an inguinal LN incased in fat), cutting the fatty tissue can be a challenge too. I have heard you can try and cut it thicker, but some doc's don't like that. Another thing we tried was fixing the fat for about 15 minutes in Pen-Fix (or some other alcoholic formalin mixture), if you have this much time then that can also help. Good Luck!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, December 22, 2010 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Specimen Handling Hi HistoLand, I have a question regarding frozen specimen handling. I have a group that gave me hearts, brown fat and white fat and there are some issues with the morphology of the tissue and also the cutting quality. Unfortunately I was part of the whole process from tissue harvesting to cutting and staining of specimens. We placed harvested tissue in a cryo mold with OCT and snap froze the block in LN2 and then placed on dry ice. I know sometimes the quick freeze can cause some effects but was wondering how others are addressing the freezing of tissue, especially fat. We have frozen fat this way before and did not have the same issues. Any help or suggestions would be great. Thanks!!! Kind Regards! John Shelley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problems with histonet
I haven't received anything since Wednesday, but my email is blowing up now with Histonet stuff! I thought it was just my computer =) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, December 03, 2010 6:54 PM To: histonet Subject: [Histonet] Problems with histonet I just made 2 posts today and they are not showing up. Is any one having problems too? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] sentinel nodes
Crystals maybe? How cold is the cryostat? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of King, Laurie Sent: Wednesday, December 01, 2010 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sentinel nodes Good morning all, I've been having a strange artifact on sentinel nodes, in only one lab of the two where I perform frozen sectioning services. This artifact does not show up any any other tissue type either. The node is usually almost totally covered with a bubbling, like the tissue is not laying flat. There can be intermittent unaffected areas. These aren't round bubbles, more rectangular and almost orderly in their pattern. I can do nodes in the other lab and they are fine. A colleague here was wondering if the tissue came in saline or some other liquid, which it hasn't. Any ideas? Laurie King HT (ASCP __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Histology Consultant
Thanks Nathan!! I just don't want to be cheated on what I will be getting paid. My old job is wanting me to come in and do the small amount of histology work they have. This would all be on evenings and weekends. It is not PRN, more like call me once a month and I will come do the work. I'm getting anywhere from $17/hr (that's insane!! How do you even sleep at night paying a registered, college educated person that? Working at Walmart would pay you more!!!) to $50/hr (which was more what I was thinking). Just trying to get a good feel, and not cheat myself or the other company. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Thursday, November 25, 2010 6:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histology Consultant If you don't know what the going rate is, that is exactly why you ask. That is what this forum is for. A little professionalism is appreciated. Nathan Jentsch BS HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processor change
How many blocks do you guys normally process before you change the solutions in the processor? Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Consultant
What is the going rate for a working histology consultant in Texas? For instance go in and process, embed, cut, and stain slides when needed? Thanks Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 70% alcohol
Hello all!! I moved jobs, but am still here in 80 degree Austin weather... Question. If tissue has been stored in 70% alcohol for up to a year, does there need to be anything done to it before processing? I am assuming that some rehydration might be necessary? If not can you just throw it on the processor on a normal cycle? Thanks for you help!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Salary range
Hello all, Does anyone know what the salary range for a histology supervisor is in the Austin Texas area? Thanks Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] unstained paraffin tissue slides storage--why cold?
-70 or -80 seems a little extreme to me, that's why I always just leave them in a normal freezer (-20). I think the main point of doing this from what I understand is so that the antigens stay viable. I know over time they can degrade and so your stain won't work with some antibodies. The weirdest part to me has always been that you don't have to store the blocks this way. So I think that was your question, if the blocks aren't stored in a freezer why store the slides? Won't the antigens in the blocks start to degrade as well? This is a question I would like to know the answer to as well... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] unstained paraffin tissue slides storage--why cold? From: Emily Sours talulahg...@gmail.com Date: Thu, November 04, 2010 7:07 am To: histonet@lists.utsouthwestern.edu Can I ask what the point of storing paraffin sections in freezing cold storage? They are wax sections, which never see any type of cold, so I don't understand the point of this. I do understand putting them at 4 degrees to prevent mold, but -80 seems excessive. We have kept our slides at room temperature for years and years, but these slides do not have an albumin coat (which I can see getting moldy), just a chemical coating. Fixing for paraffin and paraffin infiltration seems to keep antigens safe without refrigeration because it's so intense, but that's just conjecture on my part. Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] unstained paraffin tissue slides storage
Plain slide boxes is ok. I think you can store them for up to a year in a fridg. (4 degrees), but I usually pu them in a freezer (-20). Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] unstained paraffin tissue slides storage From: Pop Elena med_l...@yahoo.com Date: Wed, November 03, 2010 1:21 pm To: histonet@lists.utsouthwestern.edu Hello, I found here a few disscussions regarding the storage of tissue slides but I did not find a clear answer to the questions I have. I would really appreciate an answer from anybody that has experience with this. I need to store for long term a bunch of unstained tissue slides for the purpose of doing immunostaining even in a few years from now on. Unfortunatelly they were stored for about 3 years at room temperature. What it is usually recomended: to store them at -20 degrees Celsius? If yes, is it OK to store them in the regular 100 slides boxes? And when you need to start an immunostaining just take them out of the freezer and let them at room temp for a while before starting the stain or what procedure do you use? I heard some labs keep them in nitrogen gas containers. Do you have any info about this? Any imput is appreciated! Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] gram stain/label issues
Maybe try handwriting with a pencil then putting the label on aft
erwards.
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
= span style=color: rgb(51, 102, 255);8201 East Riverside Dr.
Bldg 4 Sui= te 100
Austin, Texas 78744
(512)386-2907
Original Message
Subject: [Histonet] gram stain/label issues
From: Jacqueline Farnsworth [1]jacqueline.farnswo...@cls.ab.ca
Date: Tue, October 26, 2010 1:56 pm
To: [2]histo...@lists.ut= southwestern.edu
[3]histo...@lists.uts= outhwestern.edu
HI all,
We are doing gram stains manually, and the acetone is removing some of
the = 'ink' from the printed labels making them difficult to read--let
alone scan= the barcode. Does anyone have any suggestions/hints on
what they are usin= g for their gram stain that may lessen the acetone
removal of the print on = the labels? Is there a system that will not
affect the slide labels? Is the= re a gram stain that anyone could
recommend that does not use acetone?
Thank you in advance,
Jacqueline
Jacqueline Farnsworth
Anatomic Pathology, Tech III
Diagnostic Scientific Centre
Calgary Laboratory Services
Phone: 403-770-3588
Pager: 403-212-8223 X07630
P Please consider the environment before printing this email.
This message and any attached documents are only for the use of the
intende= d recipient(s), are confidential and may contain privileged
information. An= y unauthorized review, use, retransmission, or other
disclosure is strictly= prohibited. If you have received this message
in error, please notify the = sender immediately, and then delete the
original message. Thank you.
___
Histonet mailing list
[4]histo...@lists.utsouth= western.edu
[5]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3Dmailto:jacqueline.farnswo...@cls 2.
3Dmailto:Histonet@lists.utsouthwestern.edu;
3. 3Dmailto:Histonet@lists.utsouthwestern.edu;
4. 3Dmailto:Histonet@lists.utsouthwestern.edu;
5. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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RE: [Histonet] A Good Human Brain Control
American Matertech Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] A Good Human Brain Control From: Candice Smoots candice_cami...@yahoo.com Date: Mon, October 25, 2010 9:08 am To: histonet@lists.utsouthwestern.edu Hi Histonetters, I was wondering if any one could tell me where can I find a human brain amyloid control. I just need some suggestions on where to order them ( control slides) from. Please feel free to list any suggestions. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Need opinions on HE stainers please!
For the love of your sanity (and keeping your pathologists happy)= DO
NOT BUY A SYMPHONY Their reagents are all proprietary and so
troubleshooting is almost impossible without paying Ventana to have
tech he= lp loom around your lab. The slides take 3 days or so to dry
before y= ou can file them, so you have to have tons of counterspace
(depending on yo= ur volume) to let them sit there in slide folders
before you can file. = ; The pathologists I worked with when we were
trying this thing out hated t= he staining and said that it wasn't
consistent from slide to slide. N= ot to mention the physical size of
the thing and the reagents are insane ex= pensive!!!
Just my opinion =)
Sarah Goebel, B.A., H= T (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
= em
Austin, Te= xas 78744
(512)386-2907
Original Message
Subject: [Histonet] Need opinions on HE stainers please!
From: Kendall Neely [1]nee...@s= hands.ufl.edu
Date: Fri, October 22, 2010 7:31 am
To: [2]histo...@lists= .utsouthwestern.edu
Our laboratory will be purchasing new HE stain equipment in the near
f= uture. We would like to hear the pros and cons out there for the
Thermo-Sh= andon Gemini, Sakura Prisma, Leica Autostainer XL and the
Ventana Symphony.= Thanks in advance for your input.
Kendall A. Neely
Histology Technical Specialist
Shands Rocky Point Laboratories
(352) 265-0111, x72113
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References
1. 3Dmailto:nee...@shands.ufl.edu;
2. 3Dmailto:histonet@lists.utsouthwestern.edu;
3. 3Dmailto:Histonet@lists.utsouthwestern.edu;
4. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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RE: [Histonet] Cheap slide scanner for rat brains?
Do you have a dissecting scope you could use? You can grab one with a pretty low resolution camera for about $800. If you need more details let me know. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Cheap slide scanner for rat brains? From: Caroline Bass cb...@wfubmc.edu Date: Fri, October 22, 2010 10:50 am To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Hello Everyone, Does anyone have a good way to get low magnification images from slides? I have some DAB stained rat coronal sections that I would like to digitize. Basically, I want to have a picture of the entire section. It doesn¹t have to be high resolution, just enough to make out the basic structures. Can someone recommend a way to do this? I¹ve used a flat bed scanner in the past, and I know some folks use an actual film slide scanner. What I¹m interested in are tips for finding a solution, particularly what to avoid, and model numbers that people have used. I don¹t want to keep buying scanners until I found one that works. And obviously I¹d like to keep the expenses to a minimum. Finally, has anyone tried this scanner? It¹s a little pricey for me, but I do like the idea behind it, although I wonder if it¹s just one of their stock scanners that they have repainted, added an adapter and jacked up the price for. It looks identical to other scanners that they sell for $300. http://www.meyerinst.com/html/oem/pse4/ Thanks, Caroline ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Recommendations for decal solution
Best decal in the world...Formical-4. It has formalin in it so it fixes and decals at the same time. You can get plain Jane decal too. The company is called decal. Here is the website. I think you can also get it from Fisher? http://www.decal-bone.com/ Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Recommendations for decal solution From: Lewis, Patrick patrick.le...@seattlechildrens.org Date: Fri, October 22, 2010 1:06 pm To: Histonet@lists.utsouthwestern.edu Hi guys, Can anyone recommend a good decal solution. I have some bone marrow and trachea tissues for paraffin sectioning and I want to decal them. Thanks Patrick Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Human anti human
Hey ya'll I was going through the archives to try and find a solution to this, but the last post was in 2008, so I thought I would throw it back out there. I need a human anti human polymer...is there one yet? I looked and couldn't find one through my normal places. As to not start the conversation that is on the archive up again. I have tried several approaches already to no avail. I tried FITC, I conjugated the primary human anti human with HRP, I've tried TSA (and tons of other companies version of TSA)...no luck... My antibody just isn't staining...I even tried putting it on the tissue and PBMCs at a 1:1 dilution...nope...any thoughts out there? I want to rip my hair out Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Un related to actual histology
Roll Roll Roll in the hay =) One of my all time favorite movies!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] Un related to actual histology From: Emily Sours talulahg...@gmail.com Date: Mon, October 18, 2010 3:37 pm To: Ann Bennett ann.bennett...@yahoo.com, histonet@lists.utsouthwestern.edu I don't know if you're a fan of Mystery Science Theater 3000, but you could be Dr Clayton Forrester! Or you could be Dexter or House. Dexter would be fun. Better yet, be Frankenstein, the doctor. That'll confuse people who aren't aware the monster had no name. Or even, FrankenSTEEN. Stab a scapel in your thigh for full effect. Also bring a recording of horses neighing so you can say Frau Bleuker! Emily -- Outside of a dog, a book is man's best friend. Inside of a dog it's too dark to read. --Groucho Marx On Mon, Oct 18, 2010 at 5:24 PM, Ann Bennett ann.bennett...@yahoo.comwrote: Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fwd: IHC coverglassing
Absolutely!! After the buffer rinse (after the counterstain), rinse in water, then 95, then 100, then xylene, and coverslip away!! I do it everyday and it works fine =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Fwd: IHC coverglassing From: Jen Campbell jcampbell_...@frontier.com Date: Tue, October 19, 2010 10:16 am To: histonet@lists.utsouthwestern.edu Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 - Forwarded Message - From: Jen Campbell jcampbell_...@frontier.com To: histonet histonet-boun...@lists.utsouthwestern.edu Sent: Tuesday, October 19, 2010 11:16:38 AM Subject: IHC coverglassing Greetings to all the histonet followers!! A question was recently brought forth in the lab I work at. Can non-automated IHC slides be dehydrated and cleared(xylene substitute) after staining and be placed on an automated coverglasser? Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Frozen sections
Chatter can also be caused by your blade angle. Try making the angle more obtuse, just don't over do it or you will get venitian (sp?) blind. The temperature is also a little cold. Try putting your thumb on the chuck, on top of the tissue, for a few sections before cutting and see if that helps any. If the tissue is necrotic, it's kind of like fat...not much you can do? Good Luck!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Frozen sections From: Maria Katleba maria.katl...@stjoe.org Date: Tue, October 19, 2010 10:27 am To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Question: When doing frozen section on a lung mass (not fatty- just regular), and it chatters, what is the cause? The cryostat was set at -26, we used the normal OCT media, and cut at 5microns. I prefer not so cold (like -23 to -24) and I cut sections at 4, 5 , and even 6 to see if it would help... still chatter! The pathologist said it looked all necrotic-like!! Without knowing for sure if the tissue's morphology is due to its real state (ie- full of cancer) or a cause of freezing artifact, I need some help from any histotech that is a Frozen Section Guru :) Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette Marking
I've used this one too, the tip is very tiny, but the pen is awesome! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: RE: [Histonet] Cassette Marking From: Maria Katleba maria.katl...@stjoe.org Date: Tue, October 19, 2010 11:34 am To: Nita Searcy nsea...@swmail.sw.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Try www.MercedesMedical.com item # MER MARKER called Platinum Marker These pens are dark and keep their colour, pens outlast the Secureline marker and this company is very competitive with the price of this marker Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, October 19, 2010 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Marking Importance: High ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Plain Vanilla Autostainer?
What about just a simple old school ski stainer? Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Plain Vanilla Autostainer? From: Johnson, Kevin kjohn...@med.miami.edu Date: Tue, October 19, 2010 11:50 am To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu I am a simple man in a simple lab with simple needs. I work in a multiple-lab research environment, typically providing paraffin/frozen sections + HE to individuals who then perform their own manual IHC. A simple man, yes, but also a busy man, who needs to be freed from staining dishes in order to pursue other tasks. What we need: (1) Deparaffinization only; (2) Deparaffinization -- HE; (3) HE only system, preferably with (4) a relatively small, preferably benchtop footprint (current unit lives in a 30 X 33 X 34 vented workstation). Automatic coverslipping extremely optional. What we have: Thermo Shandon Varistain 24-4, which performed these mundane tasks admirably until going electronically insane. It's been denied a service contract, the production line has been discontinued and the writing is on the wall. What seem to be available: Special staining systems with a whole lot of bells and whistles, designed primarily for a high-throughput hospital setting. Is there anything out there that would serve my needs, even if it is a fancy system that would be slumming it here? Or is mine just too obsolete a market? Price up to $15K, slightly negotiable. (Bench space only slightly negotiable; floor model if I must.) Many thanks, Kevin Johnson University of Miami Diabetes Research Institute Miami, FL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette Marking
As compared to a lawsuit, yes it's cheaper. But, when trying to explain to a budget committee that you need something to label things that you can do by hand...they usually don't see the point. This brings me back to my original point of mislabelling things 2 times in a year... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: Re: [Histonet] Cassette Marking From: Victor Tobias vic...@pathology.washington.edu Date: Tue, October 19, 2010 1:56 pm To: histonet@lists.utsouthwestern.edu I'm somewhat surprised that many labs are still handwriting blocks and slides. If you are using a LIS, can it integrate printing blocks and slides? Is the cost too high to add the printing capability? The cost of equipment is so cheap compared with one lawsuit. It would also reduce the stress of loosing your job over a labeling mistake. Just seems like a win win for everyone. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. On 10/19/2010 1:38 PM, Sean McBride wrote: Nita, We use HistoTec pens by Newcomer Supply ~Sean On Oct 19, 2010, at 2:22 PM, Nita Searcy wrote: ** High Priority ** If you HAVE to manually mark cassettes - what are you using? Cassette pens ? Pencils ? What is the rest of the world doing? Anything else on the market? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 Nita Searcy.vcf___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eosin
Once upon a time I heard that eosin fluoresces is this true? = ; What color does it show up? Could this be used as a sort of back gr= ound stain so you can tell exactly how many cells are in the field? Sarah Goebel, B.A., HT (ASCP) Histotechnician = = XBiotech USA Inc. 8201 East Riversid= e Dr. Bldg 4 Suite 100 Austin, Texas= 78744 (512)386-2907 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Un related to actual histology
I dressed up as Abby from CSI last year. Black wig, bright red lipstick, and a lab coat is all you need =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Un related to actual histology From: Ann Bennett ann.bennett...@yahoo.com Date: Mon, October 18, 2010 2:24 pm To: histonet@lists.utsouthwestern.edu Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Un related to actual histology
Actually it's NCIS... =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: RE: [Histonet] Un related to actual histology From: sgoe...@xbiotech.com Date: Mon, October 18, 2010 2:28 pm To: Ann Bennett ann.bennett...@yahoo.com Cc: histonet@lists.utsouthwestern.edu I dressed up as Abby from CSI last year. Black wig, bright red lipstick, and a lab coat is all you need =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 Original Message Subject: [Histonet] Un related to actual histology From: Ann Bennett ann.bennett...@yahoo.com Date: Mon, October 18, 2010 2:24 pm To: histonet@lists.utsouthwestern.edu Hello everyone! Our lab is having a laboratory related costume contest for Halloween. I'd love to hear your ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Immunohistochemistry images
Are you wanting to save your specific images, or just need a refe
rence guide for QC? Photobucket or Snapfish are two sites that are
pr= etty cheap if not free?
Sarah Goebel, B.A., HT (ASCP)
Histotec= hnician
XBiotech USA Inc.
= /div
8201 East Riverside= Dr. Bldg 4 Suite 100
Austin, Texas 78744
(= 512)386-5107
Original Message
Subject: RE: [Histonet] Immunohistochemistry images
From: Blazek, Linda [1]lbla...@digestivespecialists.com
Date: Thu, October 14, 2010 9:04 am
To: 'Michele Carr' [2]micheleca= r...@yahoo.com,
[3]histo...@lists.utsout= hwestern.edu [4]
histonet@lists.utsouthwestern.edu
Try the web site of the vender that you get your antibodies from.
-Original Message-
From: [5]histonet= -boun...@lists.utsouthwestern.edu
[[6]mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Michele Carr
Sent: Thursday, October 14, 2010 11:41 AM
To: [7]histo...@lists.uts= outhwestern.edu
Subject: [Histonet] Immunohistochemistry images
Hi everyone, I was wondering if anyone knew of a website that I can
view an= d
save the IHC images. We are putting together a new procedure manual
a= nd the
pathogists want images of the antibodies we use to be included in the
manua= l.
Thanks in advance for your responses.
Michele Carr HTL ASCP
Medical Laboratory Services
Murrieta Ca
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References
1. 3Dmailto:lbla...@digestivespecialists.co 2.
3Dmailto:michelecar...@yahoo.com;
3. 3Dmailto:histonet@lists.utsouthwestern.edu;
4. 3Dmailto:histonet@lists.utsouthwestern.edu;
5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
6. 3Dmailto:histonet-boun...@l 7.
3Dmailto:histonet@lists.utsouthwestern.edu;
8. 3Dmailto:Histonet@lists.utsouthwestern.edu;
9. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
10. 3Dmailto:Histonet@lists.utsouthwestern.edu;
11. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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RE: [Histonet] negative controls
Pretty sure you have to run your positive with your negative to keep the conditions 100% the same. You don't have to put them on the same slide necessarily, but they need to be on the same run. Also, say you are running 15 HP patient slides, you can have one negative control for all of these as long as it is on the same run. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 Original Message Subject: [Histonet] negative controls From: Victoria Baker bakevicto...@gmail.com Date: Fri, October 15, 2010 7:25 am To: Histo Net list server HistoNet@lists.utsouthwestern.edu Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
Why do you need a negative control for each block if you are runn= ing
the same antibody on each patient block? Is it just for case by c ase
reference so the negative is filed with the patient slide? Why
co= uldn't you have a control slide bank that was dated so all the
slides you d= id on that day, on that run, could be referenced back to
that control? = ; Just curious?
Sarah Goebel, B.A., HT (ASCP)
Histotechnician= br
XBiotech USA Inc.
8201 East Riverside Dr. Bld= g 4 Suite 100
Austin, Texas 78744
(512)386-= 5107
Original Message
Subject: RE: [Histonet] negative controls
From: Sebree Linda A [1]lseb...@= uwhealth.org
Date: Fri, October 15, 2010 8:08 am
To: Victoria Baker [2]bakevict= o...@gmail.com, Histo Net list
server
[3]histo...@lists.uts= outhwestern.edu
We run negative controls on every block of a case within the same run.
On autopsy cases, we only run 1 negative per tissue type, within the
same run...this is the only exception to the rule of 1 negative per
block.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [4]histonet= -boun...@lists.utsouthwestern.edu
[[5]mailto:histon= et-boun...@lists.utsouthwestern.edu] On Behalf Of
Victoria
Baker
Sent: Friday, October 15, 2010 9:26 AM
To: Histo Net list server
Subject: [Histonet] negative controls
Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run? Also, if you are doing this and have to use a different detection
kit
how do you work the QA/QC portion of this for CAP requirements.
Thanks
Vikki
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References
1. 3Dmailto:lseb...@uwhealth.org;
2. 3Dmailto:bakevicto...@gmail.com;
3. 3Dmailto:HistoNet@lists.utsouthwestern.edu;
4. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
6. 3Dmailto:Histonet@lists.utsouthwestern.edu;
7. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
8. 3Dmailto:Histonet@lists.utsouthwestern.edu;
9. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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RE: [Histonet] negative controls
So for every HP you do, you process a control cassette with the patient
tissue cassette? That seems like alot? How do you get that many
control tissues on a daily basis? What do you do with the remaining
tissue in the control block? If you throw them away everyday, I would
be interested in some of them. How do you know what IHC stains the
pathologist is going to order to know what control tissue to fix and
process at the exact same time? We have always just had a bunch of
blocks that you cut a control from? I understand that there is
variability with processing, age, etc. not trying to be dense just still
don't understand... Most places I have ever worked have control blocks
that they cut a fresh control from everyday, then stain with the patient
tissue. If there are 3 HP cases, from what I am understanding, you guys
are saying you need 3 controls for slides that are on the same machine,
with the same reagents, same antibody, and same times. Why couldn't you
just have one for all 3 cases? Then the next day have a fresh ONE for
that day, date them, and file them. So if you needed to see the HP
control for October 15th, you could go pull the control for that day...
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] negative controls
From: Rene J Buesa rjbu...@yahoo.com
Date: Fri, October 15, 2010 8:33 am
To: Sebree Linda A lseb...@uwhealth.org, sgoe...@xbiotech.com
Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu
Because each tissue block has its own characteristics regarding fixation
and processing some of which can influence the reactivity. If you have a
bank of negative controls, how can you be sure that any of those blocks
have received exactly the same treatment and reacted in the same way to
the test block?
The same goes for any bank of positives, so that is why you should have
a positive control section in the same slide as the test section.
René J.
--- On Fri, 10/15/10, sgoe...@xbiotech.com sgoe...@xbiotech.com wrote:
From: sgoe...@xbiotech.com sgoe...@xbiotech.com
Subject: RE: [Histonet] negative controls
To: Sebree Linda A lseb...@uwhealth.org
Cc: Histo Net list server HistoNet@lists.utsouthwestern.edu
Date: Friday, October 15, 2010, 11:17 AM
Why do you need a negative control for each block if you are runn=
ing
the same antibody on each patient block? Is it just for case by c
ase reference so the negative is filed with the patient slide? Why
co= uldn't you have a control slide bank that was dated so all
the
slides you d= id on that day, on that run, could be referenced back
to
that control? = ; Just curious?
Sarah Goebel, B.A., HT (ASCP)
Histotechnician= br
XBiotech USA Inc.
8201 East Riverside Dr. Bld= g 4 Suite 100
Austin, Texas 78744
=
(512)386-= 5107
Original Message
Subject: RE: [Histonet] negative controls
From: Sebree Linda A [1]lseb...@= uwhealth.org
Date: Fri, October 15, 2010 8:08 am
To: Victoria Baker [2]bakevict= o...@gmail.com, Histo Net
list
server
[3]histo...@lists.uts= outhwestern.edu
We run negative controls on every block of a case within the same
run.
On autopsy cases, we only run 1 negative per tissue type, within the
same run...this is the only exception to the rule of 1 negative per
block.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [4]histonet= -boun...@lists.utsouthwestern.edu
[[5]mailto:histon= et-boun...@lists.utsouthwestern.edu] On Behalf
Of
Victoria
Baker
Sent: Friday, October 15, 2010 9:26 AM
To: Histo Net list server
Subject: [Histonet] negative controls
Hi
I have a hypothetical question to those who run IHC on Ventana
instruments.
Are you running your negatives with your patient/test cases or on a
separate
run? Also, if you are doing this and have to use a different
detection
kit
how do you work the QA/QC portion of this for CAP requirements.
Thanks
Vikki
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References
1. 3Dmailto:lseb...@uwhealth.org;
2. 3Dmailto:bakevicto...@gmail.com;
3. 3Dmailto:HistoNet@lists.utsouthwestern.edu;
4. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
6. 3Dmailto:Histonet@lists.utsouthwestern.edu;
7.
RE: [Histonet] Defrosting Cryostat
With one cryostat it is a little bit difficult, but not impossibl= e.
Turn it off at the end of the day (after all the possible frozen s
urgeries are done) and let it defrost overnight. Have someone come
in= an hour or so early to clean it out with 100% alcohol (or formalin
or both= ) then turn it back on. Most crostats only take about 45
minutes or s= o to get back down to temperature. If you need more
time then just co= me in earlier. Most frozen sections I have ever
dealt with never come= earlier than 730 (which that rarely happens).
Good luck!!
= div
Sarah Goeb= el, B.A., HT (ASCP)
Histotechnician
XBiotech = USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100= /div
Austin, Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] Defrosting Cryostat
From: [1]beth@hcahealthc= are.com
Date: Tue, October 12, 2010 7:01 am
To: [2]histo...@lists= .utsouthwestern.edu
How are institutions handling the requirement for defrosting the
cryostat d= uring disinfection? Do most sites have a spare cryostat to
use when one i= s defrosting or do you pay staff to come in on the
weekends to do this? I = know this requirement has been out for over a
year and hoping for some good= feedback now.
Beth A. Fye, CT (ASCP)
Pathology Technical Manager
HCA Richmond Hospital Laboratories
office: (804)228-6564
fax: (804)323-8638
[3]mailto:beth@hcahealth= care.com
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References
1. 3Dmailto:beth@hcahealthcare.com;
2. 3Dmailto:histonet@lists.utsouthwestern.edu;
3. 3Dmailto:beth@hcahealthcare.com;
4. 3Dmailto:Histonet@lists.utsouthwestern.edu;
5. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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RE: [Histonet] Defrosting Cryostat
Even with a self-defrosting cryostat, if you are regulated by CAP= you
still have to decontaminate it.
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 E= ast Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: Re: [Histonet] Defrosting Cryostat
From: Rene J Buesa [1]rjbu...@yahoo.c= om
Date: Tue, October 12, 2010 8:16 am
To: [2]beth@hcahealthcare.co= m, [3]histo...@lists.
utsouthwestern.edu,
Emily Sours [4]talulahg...@gmail.= com
The best solution is to buy a self-defrosting cryostat. They work in
the = same way as your home freezer and do not build-up ice.
René J.
--- On Tue, 10/12/10, Emily Sours [5]talulahg...@gmail.com wrote:
From: Emily Sours [6]talulahg...@= gmail.com
Subject: Re: [Histonet] Defrosting Cryostat
To: [7]beth@hcahealthcare.co= m, [8]histo...@lists.
utsouthwestern.edu
Date: Tuesday, October 12, 2010, 10:31 AM
Our cryostat is a CM3050--we let it defrost overnight with the glass
door open and it's fine the next morning. alternatively, you could
defrost= it
over a weekend. i don't know how long disinfecting takes, though, so
= this
may not be feasible for you.
also, it only takes a few hours more to get back down to the working
temperature, so it's ready to use the next day, just later, at say
noon or= br so.
Emily
--
Correction:
This blog post originally stated that one in three black men who have
sex with me is HIV positive. In fact, the statistic applies to black
men who
have sex with men.
[9]http://www.tbd.com/blogs/amanda-hess/2010/10/hiv-positive-black-g
ay-men-to-get-the-bayard-rustin-project-a-district-campaign-against-ai
ds-28= 73.html
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References
1. 3Dmailto:rjbu...@yahoo.com;
2. 3Dmailto:beth@hcahealthcare.com;
3. 3Dmailto:histonet@lists.utsouthwestern.edu;
4. 3Dmailto:talulahg...@gmail.com;
5. 3Dmailto:talulahg...@gmail.c 6. 3Dmailto:talulahg...@gmail.com;
7. 3Dmailto:beth@hcahealthcare.com;
8. 3Dmailto:histonet@lists.utsouthwestern.edu;
9. 3Dhttp://www.tbd.com/blogs/amanda-hess/2010/10/hiv-positive-black- 10.
3Dmailto:Histonet@lists.utsouthwestern.edu;
11. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
12. 3Dmailto:Histonet@lists.utsouthwestern.edu;
13. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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[Histonet] Pathologists vs. Surgeons
Hey Ya'll, A friend of mine forwarded me this. We both are HTs and have had to deal with frozens a lot on a daily basis. Thought everyone might need a good chuckle on a Monday!! Cheers!! http://www.xtranormal.com/watch/6847761/ Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] mislabeling of slides and blocks
Holy cow man!! Fired for mislabeling 2 times in 260 days!!= nbsp;
Have you never been human and made a mistake?! I could understa= nd
if it was a tech on a regular basis messing up, but that seems a
little = crazy, especially if you are in a high volume hospital!!
WOW!!
Just my opinion...
Sarah Goebel, B.A., HT (ASCP)
= div
Histo= technician
XBiotech USA Inc.
8201 East Rivers= ide Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: RE: [Histonet] mislabeling of slides and blocks
From: Mahoney,Janice A [1]= janice.maho...@alegent.org
Date: Fri, October 08, 2010 9:40 am
To: Amy Farnan [2]farn...@nehealth= .com,
[3]histo...@lists.utsout= hwestern.edu [4]
histonet@lists.utsouthwestern.edu
Cc: Dean, Sherry [5]she= rry.d...@ventana.roche.com
I'd be happy to share mine but it is very strict. People are given a
final= written warning for the first mislabel not caught in the
department and ca= n be fired for the second within a year.
I highly suggest the Ventana Vantage (there are others but in my
opinion Va= ntage is the best) bar code system for patient safety. It
also relieves th= e stress on your Histo techs because the bar coding
helps them assure they = have the correct slide, block, etc. Techs are
hard to find and if we start= firing for inevitable human error we
will cause even more tech shortages.= br When you look at all the
costs that a mislabel can incur, (law suits, repla= cing personnel,
rework, etc.) the cost of the software is not too bad.
Janice Mahoney HT(ASCP)
Histology/Cytology Coordinator
Alegent Health Laboratory
4955 F Street
Omaha, NE
(402)717-2889
fax(402)717-5231
From: [6]histonet= -boun...@lists.utsouthwestern.edu
[[7]histonet-boun...@lists.utsouthwestern.edu] On = Behalf Of Amy
Farnan [[8]farn...@nehea= lth.com]
Sent: Friday, October 08, 2010 7:14 AM
To: [9]histo...@lists.uts= outhwestern.edu
Subject: [Histonet] mislabeling of slides and blocks
Hello everyone,
I was wondering if you could share your policy for employee
infractions of = mislabeling blocks and slides? I would like to get an
idea of what the stan= dard is out there. Any help would be
appreciated.
Thank you
Disclaimer: The information in this message is confidential. If you
are no= t the intended recipient, do not disclose, copy, or distribute
this message= , and please immediately contact the sender.
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References
1. 3Dmailto:janice.maho...@alegent.org;
2. 3Dmailto:farn...@nehealth.com;
3. 3Dmailto:histonet@lists.utsouthwestern.edu;
4. 3Dmailto:histonet@lists.utsouthwestern.edu;
5. 3Dmailto:sherry.d...@ventana.roche.com;
6. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu;
7. 3Dmailto:histonet-boun...@l 8. 3Dmailto:farn...@nehealth.com;
9. 3Dmailto:histonet@lists.utsouthwestern.edu;
10. 3Dmailto:Histonet@lists.utsouthwestern.edu;
11. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
12. 3Dmailto:Histonet@lists.utsouthwestern.edu;
13. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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RE: [Histonet] GLP Compliant Laboratory
What are you needing?
Sarah Goebel, B.A., HT (ASCP)
= Histotechnician
XBiotech USA Inc.
8201 East R= iverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] GLP Compliant Laboratory
From: Karie Reaser [1]krea...@vet= .upenn.edu
Date: Fri, October 08, 2010 9:58 am
To: [2]histo...@lists.uts= outhwestern.edu
Hi,
I was wondering if anyone runs a GLP Compliant laboratory and is
willing to= advise. Thanks Have a great day all you Histonetters!
--
Karie L Reaser A.S.
New Bolton Center
University of Pennsylvania
School of Veterinary Medicine
Comparative Orthopaedic Research Laboratory
382 W Street Road
Kennett Square, PA. 19348
Phone:610-925-6278
Fax:610-925-6820
Email:[3]krea...@vet.upenn.edu
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References
1. 3Dmailto:krea...@vet.upenn.edu;
2. 3Dmailto:Histonet@lists.utsouthwestern.edu;
3. 3Dmailto:krea...@vet.upenn.edu;
4. 3Dmailto:Histonet@lists.utsouthwestern.edu;
5. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
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