[Histonet] Cryostat Shopping

[Chris England via Histonet]

Aloha Histonetters--I've missed you!

  1.  It's been a very long time since I last posted here so please forgive my 
heap of ignorance: I haven't done this in a hot minute.
  2.  I think I'm in the market for a cryostat! But it's been 20+ years since 
the last one I bought, and I have no clue what features I'll need now. Do I buy 
a reliable 90s analog model? Do I need digital control? No idea! I'd love to 
talk to sales reps to get the pitch...but because I'm most likely to buy a used 
model, I feel like I'd be wasting their time. So I thought I'd ask you fine 
people.

TL;DR: What features would you recommend are very useful for a cryostat for a 
low-volume research lab? And is it a bad idea to expect to do H and other 
stains on cryosectioned tissue?

To get the best advice, I'll try to anticipate some of the more likely 
questions and answer:
 "What do you want to do?"
Ideally, we'd like to do most of the same staining that one would do on FFPE 
tissue (H and some other stains) on fixed or unfixed frozen tissue. However 
more importantly I anticipate a lot of IHC/IF, and probably most important to 
us is preserving lipids in sectioned tissue (hence avoiding the clearing agents 
used with paraffin).
"What kind of tissue?" We're a tissue engineering company working extensively 
with mammary cells, so the workload will likely be fatty tissue, and some 
composite samples that are tissue and polymer scaffolds (nothing a good blade 
can't easily cut--I think).
"What volume?" Pretty low. I may well be underestimating the workload, but most 
likely a dozen blocks every few weeks.
"What budget?" That what I need help with. I can probably go up to $20k, and 
mgmt is OK with a new model if it's necessary, but frankly I wasn't fantastic 
on a cryostat the last time I bought one so I'm not confident I'm qualified to 
say what exactly we need.
"What is your skill level?" Noob. I've used a cryostat before, but my 
technique...definitely needs improvement. On the other hand, I'm the only one 
in my company that has any experience with histology at all, so I anticipate 
training a new generation of research histologists. So, I probably want a 
cryostat that may not do everything for me, but also won't require a lot of 
shoehorning.

I'll also leave a more open question down here: What is a paraffin microtome 
better suited to than a cryostat, and vice-versa?

Thank you so, so much in advance for your time and any help you can offer!!!

Warmest regards and aloha,
Jack England
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Re: [Histonet] Cryostat anti-roll plate

[Bob Richmond via Histonet]

>
>
> My experience with anti-roll plates is mostly with the old International
> cryostats of days gone by. Pathologists usually disdained anti-roll plates
> and used an artist's brush to keep the section from rolling, but more
> proficient users depended on them. I learned to use them in a research
> histology lab. We sat at the side of the cryostat by the crank, rather than
> in front of it, and used an anti-roll plate. It was easy to cut fifty
> slides with it, which I required for my research fifty years ago.


Bob Richmond
retired samurai pathologist
Maryville TN

>
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Re: [Histonet] Cryostat anti-roll plate

[Keyser, Geri L via Histonet]

Got it...

I need to test it. 

But, I'm going to take some parafilm, pull it over the bolt, then replace the 
screw. 

Increase that friction. 

Geri 

-Original Message-
From: Keyser, Geri L via Histonet  
Sent: Wednesday, May 25, 2022 9:59 AM
To: Anne Murvosh ; Ken Marzinsky 
; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate

Maybe there's a product that increases friction with screw threads, doesn't 
have toxic outgassing, and works at low temperatures. 

I'm going to look for something like this. 

Geri

-Original Message-
From: Anne Murvosh via Histonet  
Sent: Wednesday, May 25, 2022 8:32 AM
To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate

WARNING: This email appears to have originated outside of the UW Health email 
system.
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We have 3 new leicas and we constantly have to adjust them. They creep forward 
so much that I just automatically know to roll it slightly back after every 4 
specimens or so. The new leica designs are just bad and customer service is 
even worse. We have to use the plate as we do Mohs specimens and the sections 
are just better with the glass plate. I want to look for a different machine 
next time. We did test the Avantik and it cut nice but the glass plate was on 
the front and got in the way. I wish they would talk to Mohs techs before they 
design these. Oh well. No tricks just constant adjusting. Thanks Anne

-Original Message-
From: Ken Marzinsky via Histonet 
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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Re: [Histonet] Cryostat anti-roll plate

[Keyser, Geri L via Histonet]

Maybe there's a product that increases friction with screw threads, doesn't 
have toxic outgassing, and works at low temperatures. 

I'm going to look for something like this. 

Geri

-Original Message-
From: Anne Murvosh via Histonet  
Sent: Wednesday, May 25, 2022 8:32 AM
To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate

WARNING: This email appears to have originated outside of the UW Health email 
system.
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We have 3 new leicas and we constantly have to adjust them. They creep forward 
so much that I just automatically know to roll it slightly back after every 4 
specimens or so. The new leica designs are just bad and customer service is 
even worse. We have to use the plate as we do Mohs specimens and the sections 
are just better with the glass plate. I want to look for a different machine 
next time. We did test the Avantik and it cut nice but the glass plate was on 
the front and got in the way. I wish they would talk to Mohs techs before they 
design these. Oh well. No tricks just constant adjusting. Thanks Anne

-Original Message-
From: Ken Marzinsky via Histonet 
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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Re: [Histonet] Cryostat anti-roll plate

[Anne Murvosh via Histonet]

We have 3 new leicas and we constantly have to adjust them. They creep forward 
so much that I just automatically know to roll it slightly back after every 4 
specimens or so. The new leica designs are just bad and customer service is 
even worse. We have to use the plate as we do Mohs specimens and the sections 
are just better with the glass plate. I want to look for a different machine 
next time. We did test the Avantik and it cut nice but the glass plate was on 
the front and got in the way. I wish they would talk to Mohs techs before they 
design these. Oh well. No tricks just constant adjusting. Thanks Anne

-Original Message-
From: Ken Marzinsky via Histonet 
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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Re: [Histonet] Cryostat anti-roll plate

[Keyser, Geri L via Histonet]

I use roll plate. 

I don't like the roll plate on the Leica. The Leica design creeps... a lot. 

Also, with the leica, the contact point with the blade is a soft aluminum arm. 
If you make adjustments with the roll plate holder contacting the blade, it 
will damage the soft aluminum holder. 

I like the thermo design much better. It creeps a lot less. 

Here's a procedure for roll plate adjustment. 

1.  Mount block on specimen disc. 
2.  Place Specimen Disc in chuck, position Blade carrier, and face off.
3.  Turn knurling screw clockwise until sections do not pass under the 
Anti-roll plate.
4.  Turn knurling screw counter-clockwise by 20 degree increments until 
sections effortlessly pass under the Anti-Roll Plate WITHOUT hitting the block. 

*   If the block is hitting the anti-roll plate, it may cause damage to 
glass. 
*   Hard contact is visible and can be heard. 
*   Soft contact, which can also damage the Anti-roll plate, cannot be seen 
or heard. But, can be felt by gently placing finger on the anti-roll plate 
turning lever while taking a section.


Geri 


-Original Message-
From: Ken Marzinsky via Histonet  
Sent: Tuesday, May 24, 2022 3:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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[Histonet] Cryostat anti-roll plate

[Ken Marzinsky via Histonet]

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? 
Does it work well on the Leicas and Microms, or do you have to figit with it a 
lot? Does anyone have any tricks they can share to get it to work all the time? 
 I found that a little spritz of freeze spray on the plate and the bottom of 
the roll glass helps, but it is still seems a lot easier for us to use a brush 
or a teasing needle.

Ken
Durham, NC
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[Histonet] Cryostat cutting head temperatures

[Ken Marzinsky via Histonet]

I was asking about the cutting head temperature because the Leica CM3050 
literature says the cutting head can be adjusted down to -50c separately from 
the chamber temperature. Does anyone out there use this unit? It seems to be 
very popular with the research community.  Do you ever adjust the temperature 
of the cutting head down that low, or do you just adjust the chamber 
temperature? Why would you ever use this separate feature?  Why would you chose 
this unit over the 1850?

Ken
Durham, NC
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[Histonet] Cryostat vacuums

[Wooten, Jennifer via Histonet]

Does anyone use a vacuum to clean out their cryostats, either on or off-board?

Jennifer Wooten, BA, BS, HTL (ASCP)CM
Technical Supervisor | Anatomic Pathology | University Hospital

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Re: [Histonet] Cryostat validation

[Terri Braud via Histonet]

Tony - We were on the same page.  We purchased fresh beef liver, sectioned and 
froze it, cut and stained duplicate blocks and had the pathologist sign off on 
the process.  We were also able to get some tonsils delivered fresh and did 
them, too.  Terri

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
Today's Topics:

   
   2. Re: New cryostat validation procedure (Tony Henwood (SCHN))
Message: 2
Date: Mon, 23 Aug 2021 20:31:19 +
From: "Tony Henwood (SCHN)" 
To: Greg Dobbin 
Subject: Re: [Histonet] New cryostat validation procedure

Hi Greg,

I would just grab some kidneys from the butcher, freeze and section as usual. 
They should give equivalent results to the older unit.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal 
Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of 
Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead 
NSW 2145, AUSTRALIA



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Re: [Histonet] Cryostat

[Colleen Forster via Histonet]

I agree with Terri. I also have an 1850 and will use it until parts are no
longer available which could be,. like she said, years. We also have a
local company do the PM and repairs and will use it until
it absolutely cannot be repaired.

Colleen Forster HT(ASCP)QIHC
University of Minnesota
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 555455
612-626-1930

On Tue, Aug 10, 2021 at 10:47 AM Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> I'm not sure I understand why you would want to replace your 1850s just
> because Leica is closing the support.  I have 2 and they are real
> workhorses.  There are 2 independent companies in the area that can repair
> and perform PMs.  One is Bel-Air and the other is Advantik.  I'm sure there
> are others, but I've used both companies and can highly recommend them.  If
> you like your Leica, keep it until parts are no longer available, which
> could be years.
> I have two 1850s and I'm sure they will be here for a long time.  Terri
>
> Terri L. Braud, HT(ASCP)
> HNL Laboratories for
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> Ph: 215-938-3689
> Fax: 215-938-3874
> Today's Topics:
>
>1. cryostat (Histology)
>
> Date: Mon, 9 Aug 2021 13:00:30 +
> Subject: [Histonet] cryostat
> Hello,
> Trying to get everyone's opinion on cryostats.  We have had the Leica
> CM1850 forever and it is not being supported by Leica anymore.  Thinking of
> trying a different company maybe Tanner or Rankin.  What does everyone use
> and like.  We do not cut Mohs, only DIFs.  Maybe 1 a day.
> Thanks to all,
> Mehndi Helgren
> Dominion Pathology Laboratories
> 733 Boush Street
> Suite 200
> Norfolk, VA 23510
> 757-664-7901
>
>
>
> --
>
> Message: 2
> Date: Mon, 9 Aug 2021 13:56:42 +
> From: "Morken, Timothy" 
> To: Histology 
> Cc: Histonet 
> Subject: Re: [Histonet] cryostat
> Message-ID:
> <
> co1pr05mb8053d300dfe09b8f30e66acbe7...@co1pr05mb8053.namprd05.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We
> have two Epredia NX70 cryostats. We've used them for 8 years now and really
> like them.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies Department
> of Pathology UC San Francisco Medical Center
>
>
> -Original Message-
> From: Histology via Histonet 
> Sent: Monday, August 09, 2021 6:01 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] cryostat
>
> Hello,
>
> Trying to get everyone's opinion on cryostats.  We have had the Leica
> CM1850 forever and it is not being supported by Leica anymore.  Thinking of
> trying a different company maybe Tanner or Rankin.  What does everyone use
> and like.  We do not cut Mohs, only DIFs.  Maybe 1 a day.
>
> Thanks to all,
>
> Mehndi Helgren
>
> Dominion Pathology Laboratories
> 733 Boush Street
> Suite 200
> Norfolk, VA 23510
> 757-664-7901
>
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>
>
>
> --
>
> Subject: Digest Footer
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-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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Re: [Histonet] Cryostat

[Terri Braud via Histonet]

I'm not sure I understand why you would want to replace your 1850s just because 
Leica is closing the support.  I have 2 and they are real workhorses.  There 
are 2 independent companies in the area that can repair and perform PMs.  One 
is Bel-Air and the other is Advantik.  I'm sure there are others, but I've used 
both companies and can highly recommend them.  If you like your Leica, keep it 
until parts are no longer available, which could be years.
I have two 1850s and I'm sure they will be here for a long time.  Terri

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
Today's Topics:

   1. cryostat (Histology)

Date: Mon, 9 Aug 2021 13:00:30 +
Subject: [Histonet] cryostat
Hello,
Trying to get everyone's opinion on cryostats.  We have had the Leica CM1850 
forever and it is not being supported by Leica anymore.  Thinking of trying a 
different company maybe Tanner or Rankin.  What does everyone use and like.  We 
do not cut Mohs, only DIFs.  Maybe 1 a day.
Thanks to all,
Mehndi Helgren
Dominion Pathology Laboratories
733 Boush Street
Suite 200
Norfolk, VA 23510
757-664-7901



--

Message: 2
Date: Mon, 9 Aug 2021 13:56:42 +
From: "Morken, Timothy" 
To: Histology 
Cc: Histonet 
Subject: Re: [Histonet] cryostat
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two 
Epredia NX70 cryostats. We've used them for 8 years now and really like them. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center


-Original Message-
From: Histology via Histonet 
Sent: Monday, August 09, 2021 6:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat

Hello,

Trying to get everyone's opinion on cryostats.  We have had the Leica CM1850 
forever and it is not being supported by Leica anymore.  Thinking of trying a 
different company maybe Tanner or Rankin.  What does everyone use and like.  We 
do not cut Mohs, only DIFs.  Maybe 1 a day.

Thanks to all,

Mehndi Helgren

Dominion Pathology Laboratories
733 Boush Street
Suite 200
Norfolk, VA 23510
757-664-7901

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Re: [Histonet] cryostat

[Haiyan via Histonet]

Hi Mehndi,

Same here, I had been using Epredia cryostat for the last several years. I
like it very much.  A video on youtube shows its beautiful features.
https://youtu.be/oMNt6RT5cWI

Best,
Haiyan

On Mon, Aug 9, 2021 at 7:04 AM Morken, Timothy via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We
> have two Epredia NX70 cryostats. We've used them for 8 years now and really
> like them.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
> -Original Message-
> From: Histology via Histonet 
> Sent: Monday, August 09, 2021 6:01 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] cryostat
>
> Hello,
>
> Trying to get everyone's opinion on cryostats.  We have had the Leica
> CM1850 forever and it is not being supported by Leica anymore.  Thinking of
> trying a different company maybe Tanner or Rankin.  What does everyone use
> and like.  We do not cut Mohs, only DIFs.  Maybe 1 a day.
>
> Thanks to all,
>
> Mehndi Helgren
>
> Dominion Pathology Laboratories
> 733 Boush Street
> Suite 200
> Norfolk, VA 23510
> 757-664-7901
>
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Re: [Histonet] cryostat

[Morken, Timothy via Histonet]

Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two 
Epredia NX70 cryostats. We've used them for 8 years now and really like them. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Histology via Histonet  
Sent: Monday, August 09, 2021 6:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat

Hello,

Trying to get everyone's opinion on cryostats.  We have had the Leica CM1850 
forever and it is not being supported by Leica anymore.  Thinking of trying a 
different company maybe Tanner or Rankin.  What does everyone use and like.  We 
do not cut Mohs, only DIFs.  Maybe 1 a day.

Thanks to all,

Mehndi Helgren

Dominion Pathology Laboratories
733 Boush Street
Suite 200
Norfolk, VA 23510
757-664-7901

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[Histonet] cryostat

[Histology via Histonet]

Hello,

Trying to get everyone's opinion on cryostats.  We have had the Leica CM1850 
forever and it is not being supported by Leica anymore.  Thinking of trying a 
different company maybe Tanner or Rankin.  What does everyone use and like.  We 
do not cut Mohs, only DIFs.  Maybe 1 a day.

Thanks to all,

Mehndi Helgren

Dominion Pathology Laboratories
733 Boush Street
Suite 200
Norfolk, VA 23510
757-664-7901

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Re: [Histonet] Cryostat

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet]

Hi Brittany,
Personally I do not like the HM525NX.  It is awkward and difficult to control 
with no manual advance except the control panel.  When advancing /trimming you 
are never certain how far forward the block will travel.  The UV light fitting 
in the lid always seems to get in the way of my field of view.  Ours doesn't 
have temperature control of the block holder which can make sectioning more 
difficult.

Looking at the specs of the cryostar I would actually opt for the NX70.

Kind regards,
Penny :)

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.m...@nhs.net

0300 131 4500 ext 734914
0300 131 4914

-Original Message-
From: Brittany Hethcox [mailto:bheth...@hcmhcares.org]
Sent: 19 May 2021 15:21
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker,
Rhonda Ford. She officially retired and I am so happy for her! With that
being said, I am in a pickle because the cryostat we had completely broke
down. We were having issues with it during my training period, so I never
officially got to use it. We are now looking for a new cryostat for our
department. My supervisor is asking me about different models and features,
but I don't know much about any of this. So I was hoping you all could
help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically
the "Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our
purchase (e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

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Re: [Histonet] Cryostat

[Morken, Timothy via Histonet]

Guess I missed your other questions. 

I think the fog mist (Cold D) may be better - it reaches all area of the 
chamber. UV light is shaded from some areas, though probably hits most areas 
you might be touching. 

The misting requires a specific solution, not just anything. Hydrogen peroxide 
is the disinfecting agent. 

Other features:
We have the vacutome on the original units but we never use it. We do all 
kidney and muscle sectioning and these small samples do not produce much 
debris. On our new replacement unit (to replace a 7-year old NX70) we opted out 
of the vacuum.  If using in a gross room/Or setting the vacuum may be very 
useful. It only adds a hose and suction manifold in the unit and does not get 
in the way of anything. 




Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Morken, Timothy via Histonet  
Sent: Wednesday, May 19, 2021 8:26 AM
To: Brittany Hethcox 
Cc: Histonet 
Subject: Re: [Histonet] Cryostat

Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're 
reliable and easy to use. We have the elevator on ours and it makes a 
difference for different size people in the lab. It also can have a vapor 
disinfectant system which we use and it works great. 

We use ours for kidney and muscle cryosectioning, not gross room/OR sections 
but these would work fine for that. They have a lot of room inside for 
specimens. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center


-Original Message-
From: Brittany Hethcox via Histonet 
Sent: Wednesday, May 19, 2021 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker, Rhonda 
Ford. She officially retired and I am so happy for her! With that being said, I 
am in a pickle because the cryostat we had completely broke down. We were 
having issues with it during my training period, so I never officially got to 
use it. We are now looking for a new cryostat for our department. My supervisor 
is asking me about different models and features, but I don't know much about 
any of this. So I was hoping you all could help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically the 
"Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones 
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our purchase 
(e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

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Re: [Histonet] Cryostat

[Morken, Timothy via Histonet]

Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're 
reliable and easy to use. We have the elevator on ours and it makes a 
difference for different size people in the lab. It also can have a vapor 
disinfectant system which we use and it works great. 

We use ours for kidney and muscle cryosectioning, not gross room/OR sections 
but these would work fine for that. They have a lot of room inside for 
specimens. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Brittany Hethcox via Histonet  
Sent: Wednesday, May 19, 2021 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker, Rhonda 
Ford. She officially retired and I am so happy for her! With that being said, I 
am in a pickle because the cryostat we had completely broke down. We were 
having issues with it during my training period, so I never officially got to 
use it. We are now looking for a new cryostat for our department. My supervisor 
is asking me about different models and features, but I don't know much about 
any of this. So I was hoping you all could help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically the 
"Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones 
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our purchase 
(e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

--
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[Histonet] Cryostat

[Brittany Hethcox via Histonet]

Hello everyone!

I am new to the Histology world. You all may know my previous coworker,
Rhonda Ford. She officially retired and I am so happy for her! With that
being said, I am in a pickle because the cryostat we had completely broke
down. We were having issues with it during my training period, so I never
officially got to use it. We are now looking for a new cryostat for our
department. My supervisor is asking me about different models and features,
but I don't know much about any of this. So I was hoping you all could
help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically
the "Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our
purchase (e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

-- 
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

-- 
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[Histonet] Cryostat Samples for Molecular Testing

[Cooper, Brian via Histonet]

Good afternoon Histonet!

We would like to know what decontamination procedures you are performing to 
prevent cross-contamination in between cutting scrolls for frozen samples for 
molecular testing.  We've had a few complaints of trace carryover from one 
sample to the next.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Cryostat cooling time

[Lynn Whitmore via Histonet]

pient, please contact the sender by reply e-mail 
>immediately.  Please destroy all copies of the original message and all 
>attachments. Your cooperation is greatly appreciated.
>Columbus Regional Hospital
>2400 East 17th Street
>Columbus, Indiana 47201
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>[https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg]
>
>--
>
>Message: 3
>Date: Wed, 4 Sep 2019 14:44:20 -0400
>From: Victoria Baker 
>To: "Knutson, Deanne" 
>Cc: Histo Net list server 
>Subject: Re: [Histonet] CPT Code 88172
>Message-ID:
>   
>Content-Type: text/plain; charset="UTF-8"
>
>Hi,
>
>I just referred back to my sometimes frustrating clear as mud 2019 CPT
>coding binder.
>88172 is not saying that it has to be done by a pathologist.  In many cases
>it is completed by a Cytotechnologist.
>The precise wording says
>"Cytopathology, evaluation of fine needle aspirate; immediate
>cytohistologic study to determine adequacy for diagnosis, first evaluation
>episode each site
>The professional code comes in on 88173 where it involves the
>interpretation and report.
>
>The stance that it is a tech component comes from the
>Cytologist/pathologist obtaining the specimen.  However they are
>responsible for the adequacy of of the specimen.  The argument goes both
>ways.
>
>At this institution we use it as a professional code only.
>
>Vikki
>
>On Wed, Sep 4, 2019, 11:43 AM Knutson, Deanne via Histonet <
>histonet@lists.utsouthwestern.edu> wrote:
>
>> Hello Histonetters!
>>
>> I am wondering how others may be using the cpt code 88172-T?  Like in what
>> type of scenarios?
>>
>> Thank you for your responses.
>>
>> Deanne Knutson
>> Supervisor
>> Anatomic Pathology
>> dknut...@primecare.org<mailto:dknut...@primecare.org>
>>
>>
>>
>>
>>
>>
>>   
>> This email may include confidential and privileged information. If this is
>> not intended for your use, please destroy immediately and contact the
>> sender of the message.
>>
>> This email and attachments contain information that may be confidential or
>> privileged. If you are not the intended recipient, notify the sender at
>> once and delete this message completely from your information system.
>> Further use, disclosure, or copying of information contained in this email
>> is not authorized, and any such action should not be construed as a waiver
>> of privilege or other confidentiality protections.
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>>
>
>
>--
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>Message: 4
>Date: Wed, 4 Sep 2019 19:47:30 +
>From: "Moe, Barbi A" 
>To: "histonet@lists.utsouthwestern.edu"
>   
>Subject: [Histonet] PPE for frozen sections
>Message-ID:
>   
> 
>   
>Content-Type: text/plain; charset="us-ascii"
>
>Could anyone share what PPE you are required to wear when performing a frozen 
>section?
>
>If doing a frozen section on a piece of lung or mediastinal nodes are you 
>required to wear a respirator such as N95 or even a PAP-R?
>
>Any thoughts are appreciated.
>
>Barb Moe
>Histology Lab
>Gundersen Health Systems
>La Crosse WI
>ba...@gundersenhealth.org<mailto:ba...@gundersenhealth.org>
>
>
>--
>
>Message: 5
>Date: Wed, 4 Sep 2019 19:51:41 +
>From: "Moe, Barbi A" 
>To: "histonet@lists.utsouthwestern.edu"
>   
>Subject: [Histonet] DAKO Artisan special stain kit validations
>Message-ID:
>   
> 
>   
>Content-Type: text/plain; charset="us-ascii"
>
>For those that are using the DAKO Artisan for special stains could you comment 
>on how you are handling implementing new kits?
>
>When a new lot number on a kit is received do you run a positive AND negative 
>control either prior to use or concurrent with a patient case?
>
>Any thoughts are appreciated.
>
>Barb Moe
>Hist

[Histonet] Cryostat to room temperature

[Moe, Barbi A via Histonet]

Does anyone know how long it would take a cryostat that is maintained at 
approx. -22 degrees C  to come to room temperature after being turned off?

Any thoughts are appreciated.

Barb Moe
Histology Lab
Gundersen Health Systems
La Crosse WI
ba...@gundersenhealth.org

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[Histonet] cryostat vs sliding microtome

[Emily Stevenson via Histonet]

Hello,
Looking for information on using a cryostat vs a sliding microtome for
ENFD/small nerve fiber tissue cutting.

Thanks!

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[Histonet] Cryostat question

[Charles Riley via Histonet]

Does anyone out there use the Avantik QS12 cryostats? We have a machine that is 
only a year and a half old and have had to get the Peltier system (the cryobar) 
replaced 4 times. Is anyone else having this problem consistently? 

Sent from my iPhone
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[Histonet] Cryostat and Parts

[Travis Troyer via Histonet]

We are in the process of moving and we have some equipment and supplies
that we are not taking with us.We have 2 Microm HM 505E cryostats and
all of the internal components.One of the cryostats doesn't cool down
and the other one doesn't advance properly but is otherwise in working
order.If anyone is interested in the cryostats or the any of the parts
please let me know.We also have 12 bottles of Polarstat Plus cryostat
mounting media (exp 6/18).If anyone is interested please let me know.

Travis Troyer

Advanced Dermatology and Skin Cancer Center
1133 College Ave., Suite E230
Manhattan, KS 66502
Tel: 785-537-4990 <(785)%20537-4990>
Fax: 785-537-1938 <(785)%20537-1938>
d...@ksderm.com
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Re: [Histonet] cryostat decontamination

[Duddey, Aimee via Histonet]

Our last discussion with TJC to clarify their definition of "regular interval" 
was to follow the manufacturers guidelines.  I recommend determining what 
manufacturer suggests with the use of the UV decon and write that in your 
policy that addresses this standard.  If you are following their guidelines, 
you should be in compliance.

Aimee

-Original Message-
From: Nancy Schmitt via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, August 15, 2016 9:58 AM
To: '
Subject: [Histonet] cryostat decontamination

ANP.23410 states that cryostat is defrosted and wiped down at regular 
intervals.  What if you have UV decontam?  Is there a ruling on that?

Thanks

Nancy Schmitt MLT, HT(ASCP)
Dubuque, IA

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[Histonet] cryostat decontamination

[Nancy Schmitt via Histonet]

ANP.23410 states that cryostat is defrosted and wiped down at regular 
intervals.  What if you have UV decontam?  Is there a ruling on that?

Thanks

Nancy Schmitt MLT, HT(ASCP)
Dubuque, IA

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attachments. Thank you.




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[Histonet] Cryostat sectioning of frozen leaves

[Jo-Ann Bader, Ms. via Histonet]

I am trying to cut frozen leaf sections at 6 and 8 microns.  All I am getting 
is bunched up leaf.  What is the trick/  I am cutting at -20 should be cutting 
at a lower temp and cutting thicker?


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
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[Histonet] Cryostat decontamination ANP23410

[Piche, Jessica via Histonet]

Hi Everyone,
Just a quick question. If you defrost your cryostat to room temperature and 
wipe it out with 70% alcohol is that considered decontamination? Or does it 
have to be fumigated with formaldehyde, or have a UV function on board the 
machine?
Thanks,
Jessica Piche, HT(ASCP)
Waterbury Hospital



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Re: [Histonet] Cryostat Chirp

[Elizabeth Chlipala via Histonet]

Gary

I think that might have to do with the fan motor inside the unit, it might need 
to be replaced.   We had ours replaced a few years ago.  Do you service/PM the 
cryostat yearly?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Martin, Gary via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 03, 2016 2:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Chirp

My Leica CM 1850 cryostat is making a chirping sound and I can't figure out 
what is doing this. Has anyone experienced this sound, if what was the resolve. 

Thanks 

Gary

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[Histonet] Cryostat Chirp

[Martin, Gary via Histonet]

My Leica CM 1850 cryostat is making a chirping sound and I can't figure
out what is doing this. Has anyone experienced this sound, if what was
the resolve. 

Thanks 

Gary

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Re: [Histonet] Cryostat with UV disinfection

[Pfaff, Karen via Histonet]

Our Mohs Lab is CAP accredited. Every seven  days of use, cryostats are shut 
down. Once machine has been cleaned and dried we use Cavi-wipes(super 
Sani-Cloth from PDI Inc.)  We then document disinfection date on QC Log.   We 
have three cryostats, we rotate a cryostat a week for disinfecting purposes. We 
added this to our Equipment: Cryostats/Microtomes policy.
Hope this helps.
Karen Pfaff, HT (ASCP), BA
Lead Histotechnician
Skin Cancer Center
Froedtert Health
Phone: 414-805-6010    Fax: 414-805-5325


-Original Message-
From: Terri Braud [mailto:tbr...@holyredeemer.com] 
Sent: Friday, March 11, 2016 12:52 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Cryostat with UV disinfection

For those of you that are CAP accredited, and use a cryostat with UV 
disinfection, how do you get around the CAP requirement listed below? We were 
told the UV disinfection did not replace wiping down with a tuberculocidal 
disinfectant at room temp. Do you have an approved tuberculocidal disinfectant 
that will work at cryostat temps?  Curious minds would like to know!  
ANP.23410   Cryostat DecontaminationPhase II
There is a written procedure for the decontamination of the cryostat at defined 
intervals, and under defined circumstances, and decontamination records are 
evident.
NOTE:  The cryostat must be defrosted and decontaminated by wiping all exposed 
surfaces with tuberculocidal disinfectant. The cryostat should be at room 
temperature during decontamination unless otherwise specified by the 
manufacturer. This should be done at an interval appropriate for the 
institution; this must be weekly for instruments used daily. Trimmings and 
sections of tissue that accumulate inside the cryostat must be removed during 
decontamination. Although not a requirement, steel mesh gloves should be worn 
when changing knife blades. 
Terri L. Braud, HT(ASCP)





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Re: [Histonet] Cryostat with UV disinfection

[Terri Braud via Histonet]

For those of you that are CAP accredited, and use a cryostat with UV 
disinfection, how do you get around the CAP requirement listed below? We were 
told the UV disinfection did not replace wiping down with a tuberculocidal 
disinfectant at room temp. Do you have an approved tuberculocidal disinfectant 
that will work at cryostat temps?  Curious minds would like to know!  
ANP.23410   Cryostat DecontaminationPhase II
There is a written procedure for the decontamination of the cryostat at defined 
intervals, and under defined circumstances, and decontamination records are 
evident.
NOTE:  The cryostat must be defrosted and decontaminated by wiping all exposed 
surfaces with tuberculocidal disinfectant. The cryostat should be at room 
temperature during decontamination unless otherwise specified by the 
manufacturer. This should be done at an interval appropriate for the 
institution; this must be weekly for instruments used daily. Trimmings and 
sections of tissue that accumulate inside the cryostat must be removed during 
decontamination. Although not a requirement, steel mesh gloves should be worn 
when changing knife blades. 
Terri L. Braud, HT(ASCP)



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[Histonet] cryostat

[Silvia Bonner via Histonet]

Hi Everyone,

Can you recommend someone for preventative maintenance on cryostats?  We have 
both Shandon and Leica cryostats.

Thanks,

Silvia Bonner, HT(ASCP) CM
Histology Supervisor

sbon...@pathregional.com
Pathologists' Regional Laboratory
1225 Highland Ave
Clarkston, WA 99403
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[Histonet] Cryostat sectioning of FF lymph node

[Martin, Erin via Histonet]

Good morning!  My pathologists would like us to cut formalin fixed (not yet 
processed) tonsil tissue on a cryostat for DIF staining.  Has anyone done this? 
 I did a quick search that seemed to indicate that it was possible but that the 
architecture of the cells would be altered because of ice crystals and that it 
would be difficult to get the sections to stick.  If you have any advice I 
would greatly appreciate it!



Thanks,

Erin



Erin Martin, Histology Supervisor
UCSF  Dermatopathology and Oral Pathology Service
1701 Divisadero St, San Francisco, CA 94044
415-353-7248

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[Histonet] Cryostat vacuum

[Morken, Timothy via Histonet]

I just bought one from IMEB :

http://www.imebinc.com/



Hello Histonet,



Does anyone know where I can find a cryostat vacuum?  I've used them in the 
past but can't seem to find a manufacturer.



Thanks!



Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre 
General Hospital | An Affiliate of Commonwealth Health | mjdessoye at 
commonwealthhealth.net>
 | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
570-552-1486


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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[Histonet] Cryostat purchasing

[Lewis, Patrick]

Hi Everyone,

Can anyone recommend a good Cryostat to buy for use with Human Tissues.

I'd like to look for a reasonably priced model.
Also, I'd consider paying extra for a model that is designed for human tissues 
so it has disinfection ability or is easier to disinfect than older models.
I'd also consider going with a refurbished model if the price was right.

I am used to cutting on CM3000 and CM3050s which are quite old.

Thanks

Patrick Lewis
Research Associate II Bench
Seattle Childrens Research Institute
206-884-1115

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[Histonet] Cryostat maintenance personnel

[Lewis, Patrick]

Hi everyone,

Does anyone know who I would contact about servicing an old CM3000 Cryostat.

This would be in the Seattle area and we have no service contract.

I am going to call Leica tomorrow, but if anyone has information about 
repair/servicing that would be helpful.

Thanks

Patrick.



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[Histonet] Cryostat troublshooting. CM3050

[Lewis, Patrick]

H everyone,

We have been using this old CM3050 cryostat for our Frozen OCT blocks.

I am cutting at 5 uM.

My issue is that when I use the hand rotator to cut my sections it doesn't seem 
to advance with each rotation.  It can take as many as 4 rotations to go from 
one section to another.

What would cause this, and is there an easy solution to this problem?

I can use the button to automatically move the chick holder all the forward and 
back from blinking to blinking, so I am wondering if ice crystals or debris can 
be ruled out as  the issue.

Any advice welcome.

Thanks

Patrick.

ps: when I do get the blade to cut into the block, I have to wonder if the 
section I am getting is really 5 uM, or is it thicker.  Sometimes you can tell 
if the section looks particularly thick, but other times it's hard to tell.


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Re: [Histonet] Cryostat troublshooting. CM3050

[Hans B Snyder]

Hello Patrick,

We have this same cryostat except it's the CM 3050S.  Ours also has
this issue usually after it's been running for many months with a
defrost.  The typical cause of your problem is the cylinder on which
the block holder is attached has ice crystals or is frozen.  I feel
the cause of this is because the glass was left open too long while
cutting.  This allowed moister from the room to condense on the
cylinder and subsequently causes a stop go motion.

 Since I am in charge of all histology equipment maintenance, my
solution is to turn off the cryostat, take the microtome out of the
cavity and let it dry overnight.  Then in the morning, I physically
dry the microtome.  Using cryostat oil, oil all the moving parts
including the cylinder, then wipe off excess.  Before putting the
microtome back into the cavity, the cavity itself must also be
thoroughly dried.  The final cool down process usually takes about 1-2
hours.

Feel free to email any specific questions.

Hope this helps.
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com


On Wed, Jan 7, 2015 at 6:42 PM, Lewis, Patrick
patrick.le...@seattlechildrens.org wrote:
 H everyone,

 We have been using this old CM3050 cryostat for our Frozen OCT blocks.

 I am cutting at 5 uM.

 My issue is that when I use the hand rotator to cut my sections it doesn't 
 seem to advance with each rotation.  It can take as many as 4 rotations to go 
 from one section to another.

 What would cause this, and is there an easy solution to this problem?

 I can use the button to automatically move the chick holder all the forward 
 and back from blinking to blinking, so I am wondering if ice crystals or 
 debris can be ruled out as  the issue.

 Any advice welcome.

 Thanks

 Patrick.

 ps: when I do get the blade to cut into the block, I have to wonder if the 
 section I am getting is really 5 uM, or is it thicker.  Sometimes you can 
 tell if the section looks particularly thick, but other times it's hard to 
 tell.


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 of the original message.
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[Histonet] CRYOSTAT RECOMMENDATIONSRE: Histonet Digest, Vol 125, Issue 24

[Karen Kay]

Good ;Morning,
We have the Leica 1950 in our Laboratory and are extremely pleased with the 
insturment.

Karen J Kay, MLT
Supervisor - Histopathology and Cytology Laboratory
Chinook Regional Hospital - South Zone West - Alberta Health Services
Lethbridge, Alberta, Canada
karen@albertahealthservices.ca


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: April 21, 2014 11:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 125, Issue 24

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When replying, please edit your Subject line so it is more specific than Re: 
Contents of Histonet digest...


Today's Topics:

   1. RE: Xylene substitute for processing GI biopsies
  (Joe W. Walker, Jr.)
   2. Alport's testing (Houston, Ronald)
   3. Re: Xylene substitute for processing GI biopsies (Rene J Buesa)
   4. Recommendations cryostat (Pathology-Histology Sr. Supervisor)
   5. RE: Paraffin (Cameron, Elizabeth)
   6. COM 30450 (Piche, Jessica)
   7. RE: Recommendations cryostat (WILLIAM DESALVO)
   8. RE: Recommendations cryostat (Bea DeBrosse-Serra)
   9. anti mouse iNOS (Anna Hughes)
  10. Re: RE: Recommendations cryostat (Kim Tournear)


--

Message: 1
Date: Sun, 20 Apr 2014 23:36:05 +
From: Joe W. Walker, Jr. joewal...@rrmc.org
Subject: RE: [Histonet] Xylene substitute for processing GI biopsies
To: Rene J Buesa rjbu...@yahoo.com, Lori Gemeinhardt
loreli...@mac.com,histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
3c2378778400ad448ada6fd6bdb7182a0...@rrmbx03.rrmc.local
Content-Type: text/plain; charset=iso-8859-1

Are you required to revalidate all of your IHC antibodies once you switch to a 
xylene sub?

We had utilized a xylene free process in our Peloris, which utilized alcohol 
but we ran into issue with FISH for Her2 and some of our other predictive 
markers and abandoned the alcohol.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional 
Medical Center 160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
Email joewal...@rrmc.orgwww.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet 
Recognition? and the Governor's Award for Performance Excellence


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies

Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl 
alcohol) and after sectioning you can use dishwasher soap before going directly 
to the staining procedure. After staining you need nothing but an oven to dry 
out the stained sections and go diractly to coverslipping.
If you want details on the above please go to: 
http://www.histosearch.com/rene.html and read my articles on the subject, 
including processing protocols.
If you follow the instructions, you will have a totally xylene free lab.
Ren? J.
On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt loreli...@mac.com wrote:

Hi there!
I have spent a lot of time reading the archives, but still need some specific 
and current input/advice.
I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with 
a xylene substitute.  I need insight on protocols, brands, effects on 
staining. The good, the bad, the ugly. I have only used xylene in my many 
years of experience!  This is completely new (to me), and rather overwhelming.
It would be ideal to use the same substitute in the staining process, so any 
consideration with that is also appreciated.
I understand this has been discussed, at length in the past, and I 
apologize I am just hoping to zero in on the best case scenario as a 
starting point.

Thanks, in advance, for your help!
Lori

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[Histonet] cryostat design

[Kolman, Kimberly D.]

Hello all;

Is anyone aware of a cryostat design in which the microtome cabinet
would sit at bench level?  In other words, it would function like any
regular bench microtome but be housed in a cold cabinet like a cryostat?
This would enable the operator to work in a desk position instead of
uncomfortably being hunched over a cryostat cabinet.  

 

Thanks in advance,

Kim

 

 

Kimberly D. Kolman, HT  (ASCP)

VA Eastern Kansas Health Care System

4101 S 4th St Trfwy

Leavenworth KS 66048

913-682-2000x 52537

Fax: 913-758-4193

 

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Re: [Histonet] cryostat design

[Alan Bright]

Dear Kim,

Yes the Starlet cryostat made by Bright Instrument Company, it is in a small 
bench level cabinet as there is no other way of achieving good results on the 
refrigeration. Apart from freezing microtones which were highly inefficient on 
retrieving good quality thin sections.
Hope this assists you.

Alan Bright
BIC..UK

Sent from my iPhone

 On 5 Mar 2014, at 16:55, Kolman, Kimberly D. kim.kol...@va.gov wrote:
 
 Hello all;
 
 Is anyone aware of a cryostat design in which the microtome cabinet
 would sit at bench level?  In other words, it would function like any
 regular bench microtome but be housed in a cold cabinet like a cryostat?
 This would enable the operator to work in a desk position instead of
 uncomfortably being hunched over a cryostat cabinet.  
 
 
 
 Thanks in advance,
 
 Kim
 
 
 
 
 
 Kimberly D. Kolman, HT  (ASCP)
 
 VA Eastern Kansas Health Care System
 
 4101 S 4th St Trfwy
 
 Leavenworth KS 66048
 
 913-682-2000x 52537
 
 Fax: 913-758-4193
 
 
 
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[Histonet] Cryostat Disinfection

[Vickroy, Jim]

In the CAP checklist it states that The cryostat must be4 defrosted and 
decontaminated by wiping all exposed surfaces with a tuberculocidal 
disinfectant.  The cryostat should be at room temperature during 
decontamination unless otherwise specified by the manufacturer.   The newest 
cryostat by Thermofisher uses cold disinfection with a chemical called Sanosil. 
  Am I right thinking that on that cryostat we do not have to disinfect at room 
temperature?

Your thoughts?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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Re: [Histonet] Cryostat

[Hugh Luk]

Tanya and Madeline,

Jan Minshew of Leica sent a nicely detailed response to Decontaminating a 
cryostat in 
January of 2010. She apparently wrote it to put in a Leica How To manual, 
although I did
not notice it in this form.  Everything she states is well documented, although 
I also 
incorporate a known virucide and tuberculocidal product (following it's 
directions, naturally).

http://lists.utsouthwestern.edu/pipermail/histonet/2010-January/048750.html

If you have a UV cryostat, use Leica's Cryofect product or follow Tim Morken's 
previous
(2000 histosearch)formaldehyde method, they should work for killing most bugs 
too(with the 
exception of eradicating some prions like CJD/BSE).


If I am misunderstanding what you mean by Decon I apologize, as there is no 
reason why
you cannot clean up tissue debris in a cold cryostat with 100% alcohol.

Hugh
Hawaii

--
 
Date: Thu, 16 Jan 2014 10:43:09 -0800 (PST)
From: Madeline Gi madelin...@yahoo.com
Subject: Re: [Histonet] Cryostat
To: Abbott, Tanya tanyaabb...@catholichealth.net,
Histonet@lists.utsouthwestern.edu
Histonet@lists.utsouthwestern.edu
Message-ID:
1389897789.16602.yahoomail...@web121504.mail.ne1.yahoo.com
Content-Type: text/plain; charset=iso-8859-1
 
I use to decon my cryostat with 100% Alco this keeps it from freezing up and it 
clean it. 
best of luck. 
 
Madeline Rotger Milanese H.T. BSHCS
500 New Hempstead Rd.
New City N.Y. 10965
845-362-3200 Ext 129
madelin...@yahoo.com
 
 
 
On Wednesday, January 15, 2014 2:52 PM, Abbott, Tanya 
tanyaabb...@catholichealth.net wrote:
 
Need to decon my cryostat
 
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net
 
This electronic mail and any attached documents are intended solely for the 
named addressee(s) 
and contain confidential information. If you are not an addressee, or 
responsible for delivering 
this email to an addressee, you have received this email in error and are 
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copying, or disclosing this email is prohibited. If you received this email in 
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Re: [Histonet] Cryostat

[Madeline Gi]

I use to decon my cryostat with 100% Alco this keeps it from freezing up and it 
clean it. 
best of luck. 
 
Madeline Rotger Milanese H.T. BSHCS
500 New Hempstead Rd.
New City N.Y. 10965
845-362-3200 Ext 129
madelin...@yahoo.com



On Wednesday, January 15, 2014 2:52 PM, Abbott, Tanya 
tanyaabb...@catholichealth.net wrote:
 
Need to decon my cryostat

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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immediately reply to the sender and delete the message completely from your 
computer system.
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[Histonet] Cryostat Decontamination

[Abbott, Tanya]

I am looking for a procedure and product to use for decontamination after a 
potential TB case, help please!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Cryostat

[Abbott, Tanya]

Need to decon my cryostat

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] cryostat

[Webb, Dorothy L]

Does anyone have any current information on the cryostats with UV for 
decontamination?  Specifically, is it accepted as a means of decontamination by 
CAP or JS or any other regulatory agencies?  Basically, is it worth the extra 
dollars to go that route?

Much thanks and Happy 2014!!

Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist
651-254-2962



  
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[Histonet] cryostat service

[Jean Ross]

Hi everyone,

I am interested in finding out if there is anyone in or close to Delaware
that services and repairs cryostats.  Any recommendations would be much
appreciated.

Thanks,
Jean
-- 
Jean Ross
Delaware Biotechnology Institute, BioImaging Center
University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
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RE: [Histonet] cryostat service

[Rathborne, Toni]

Avantik is located in NJ and services cryostats in addition to microtomes, 
processors and stainers.
www.avantik-us.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Ross
Sent: Monday, October 28, 2013 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat service

Hi everyone,

I am interested in finding out if there is anyone in or close to Delaware that 
services and repairs cryostats.  Any recommendations would be much appreciated.

Thanks,
Jean
--
Jean Ross
Delaware Biotechnology Institute, BioImaging Center University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
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RE: [Histonet] cryostat service

[Walter Benton]

Bel Air Instruments and Dolbey Jamison

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni 
[trathbo...@somerset-healthcare.com]
Sent: Monday, October 28, 2013 2:29 PM
To: 'Jean Ross'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat service

Avantik is located in NJ and services cryostats in addition to microtomes, 
processors and stainers.
www.avantik-us.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Ross
Sent: Monday, October 28, 2013 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat service

Hi everyone,

I am interested in finding out if there is anyone in or close to Delaware that 
services and repairs cryostats.  Any recommendations would be much appreciated.

Thanks,
Jean
--
Jean Ross
Delaware Biotechnology Institute, BioImaging Center University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
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[Histonet] Cryostat cleaning

[Cynthia Baranowski]

The only method acceptable to CAP is bringing the cryostat to room temp and 
disinfecting weekly UV light will not be an acceptable alternative by CAP.
We clean out all tissue debris, wipe down with abs alcohol, bring cryostat to 
room temp, use Cavicide to disinfect, wipe down again with abs alcohol. We use 
70 % alcohol for daily cleaning.
Cindy Baranowski
Emory Healthare
Atlanta, GA

Sent from my iPad
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[Histonet] Cryostat decontamination

[anita dudley]

Just curious how often everyone is taking their cryostats down,  I know cap 
says every week if used daily.  Thanks ahead for the info.  Everyone have a 
great weekend

 

Anita Dudley

Providence Hospital

Mobile Alabama
  
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[Histonet] Cryostat cleaning

[Ann Specian]

ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a 
tuberculocidal.

Can someone tell me what they use that meets the CAP criteria listed above.

thankis, 
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RE: [Histonet] Cryostat cleaning

[Morken, Timothy]

Ann, 

There are many tuberculocidal cleaners. Just search for it. If you are in a 
hospital the central supply will most likely have some in stock.  
We use PD-128 by Spartan Chemical  

http://www.spartanchemical.com/products/product/101604


Here are some references for cryostat cleaning:

Cryostat decontamination references:

College of American Pathologists, Commentary on certification questionnaire:
1)  Decontaminate regularly with 70% alcohol.
2)  Defrost, remove trimmings and decontaminate with a tuberculocidal 
disinfectant, preferably weekly for instruments used daily
3)  Reference: National Committee for Clinical Laboratory Standards 
(NCCLS), Protection of Laboratory Workers from Instrument Biohazards and 
Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved 
Guideline M29-A. Wayne, PA, NCCLS, 1997

NCCLS site: http://www.nccls.org/


Decontamination for HIV
Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., 
Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986)
Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is 
effective against HIV).

Department of Agriculture for Northern Ireland, Safe Working and the Prevention 
of Infection in Clinical Laboratories, Health Services Advisory Committee. 
Recommends formalin fuming for 24-48 hours at room temperature. Followed by 
ammonia for one hour (presumably as a neutralizer).

Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill 
Livingstone, 1994.
Recommends defrosting , washing with 70% alcohol, complete cleaning. (no 
references given).

For those who like to read a lot:
APIC Guideline for Selection and Use of Disinfectants, Association for 
Professionals in Infection Control (APIC), American Journal of Infection 
Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in 
use and the pros and cons of each.


Tim Morken
UCSF Medical Center

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Friday, August 16, 2013 8:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat cleaning


ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a 
tuberculocidal.

Can someone tell me what they use that meets the CAP criteria listed above.

thankis, 
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RE: [Histonet] Cryostat cleaning

[Hannen, Valerie]

We wipe out the excess tisue( if there is any),  wipe down the chamber with 
abs. Alcohol then spray the chamber with CaviCide, let it sit for @ 3-5 
minutes and then wipe the chamber out again.


Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Friday, August 16, 2013 11:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat cleaning


ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a 
tuberculocidal.

Can someone tell me what they use that meets the CAP criteria listed above.

thankis,
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of the individual to
whom it is addressed and may contain information that is
privileged, confidential or otherwise exempt from disclosure
under applicable law. If the reader of this email is not the
intended recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution, or
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[Histonet] Cryostat recommendations

[Rathborne, Toni]

Happy Friday everyone!
I'm looking for some feedback on cryostat preferences. We are replacing one of 
ours now and will be evaluating a few before making a final decision. The 2 
I've looked into so far are the Leica 1860 and the HM525 by Thermo Scientific. 
I'm interested in the UV feature that many now offer too and want to know what 
your experiences are. Does it require extra maintenance? Is it costly to 
repair/replace?  Do either of these models (or previous versions) have any 
known problems? They both look very nice on the outside, but are they made of 
durable materials internally? Thanks in advance for your responses, and have a 
great weekend!

Toni

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[Histonet] Cryostat Cleaning

[Ann Specian]

Can someone tell me the CAP approved chemical that can be used to clean the 
cryostat?
thanks, Ann
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RE: [Histonet] Cryostat Cleaning

[Morken, Timothy]

Ann,

Cryostat decontamination references:

 

College of American Pathologists, Commentary on certification questionaire:

1) Decontaminate regularly with 70% alcohol.

2) Defrost, remove trimmings and decontaminate with a tuberculocidal 
disinfectant, preferably weekly for instruments used daily

3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), 
Protection of Laboratory Workers from Instrument Biohazards and Infectious 
Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline 
M29-A. Wayne, PA, NCCLS, 1997

http://www.cap.org/HTML/checklist_html/cklst08p.html

 

NCCLS site: http://www.nccls.org/

 

 

Decontamination for HIV

Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., 
Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986)

Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is 
effective against HIV).

 

Department of Agriculture for Northern Ireland, Safe Working and the Prevention 
of Infection in Clinical Laboratories, Health Services Advisory Committee. 

Recommends formalin fuming for 24-48 hours at room temperature. Followed by 
ammonia for one hour (presumably as a neutralizer).

 

Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill 
Livingstone, 1994.

Recommends defrosting , washing with 70% alcohol, complete cleaning. (no 
references given).

 

For those who like to read a lot:

APIC Guideline for Selection and Use of Disinfectants, Association for 
Professionals in Infection Control (APIC), American Journal of Infection 
Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in 
use and the pros and cons of each.


Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Tuesday, August 06, 2013 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Cleaning


Can someone tell me the CAP approved chemical that can be used to clean the 
cryostat?
thanks, Ann
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[Histonet] Cryostat Repair Service in San Diego

[dusko trajkovic]

Good Morning,
Can anyone recomend a good, reliable cryostat repair service in the San diego 
area?
thanks
Dusko
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[Histonet] cryostat decontamination

[Kolman, Kimberly D.]

Has anyone come up with a documented and referenced procedure for
decontamination of a cryostat using 37% Formaldehyde?  

 

I pulled up old notes in the Histonet archives and saw that Tim Morken
was working on this but that was a number of years ago.

 

Thanks, Kim

 

 

Kimberly D. Kolman, HT, (ASCP)

Diagnostics 115 

VA Eastern Kansas Health Care System

4101 S. 4th St. Trfwy.

Leavenworth, KS 66048

ph: 913-682-2000 x 52537/52539

 

 

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[Histonet] cryostat

[IRENA SREBOTNIK KIRBIS]

Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in 
other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw 
this in one lab!?thanksIrena 
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Re: [Histonet] cryostat

[Alan Bright]

I would not vacuum the trimmings out of cryostat, if it were infected It would 
make a good job of contaminating a laboratory etc.
Terrified Alan Bright
Sent from my iPhone



 
 
 
 
 Hi,I wonder what is the way of removing shavings/trimmings from the cryostat 
 in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I 
 saw this in one lab!?thanksIrena 
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 -- 
 

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RE: [Histonet] cryostat

[Elizabeth Chlipala]

Happy Wednesday Everyone

There are vacumes specifically made for cryostats that are hepa filtered, etc.  
We have one of those.  The company is called MARMED.  We use a shop vac for the 
paraffin microtomes, that works nicely.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alan Bright 
[abri...@brightinstruments.com]
Sent: Wednesday, February 06, 2013 9:31 AM
To: IRENA SREBOTNIK KIRBIS
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryostat

I would not vacuum the trimmings out of cryostat, if it were infected It would 
make a good job of contaminating a laboratory etc.
Terrified Alan Bright
Sent from my iPhone







 Hi,I wonder what is the way of removing shavings/trimmings from the cryostat 
 in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I 
 saw this in one lab!?thanksIrena 
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RE: [Histonet] cryostat

[Jones, Laura]

Hi Irena!

If you go to IMEB Inc.'s website, and type vacuum into the search, it will 
take you to what we use.  They sell a vacuum cleaner and accessories.  We 
currently use the filtered hoses with an old canister vacuum that's been around 
longer than I have.  When the filter gets full, we discard it in the biohazard 
trash and replace it with a new one.

Hope this helps!

Laura


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS 
[irena.kir...@hotmail.com]
Sent: Wednesday, February 06, 2013 8:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat

Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in 
other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw 
this in one lab!?thanksIrena 
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[Histonet] CRYOSTAT PROBLEM

[Lakshmi Kammili]

Hi all,
We have a old cryostat (Microm h505 model). It was making some sudden 
vibrating noises for a little while and then smooths it self. There  are no 
temperature alterations every thing was fine but suddenly it stopped working, 
no display more looks like dead :( 
I tried calling the service company which serviced before but I guess the 
company is no more in service (as it was a very old machine). I am very new to 
this maintenance issues , I would be glad if any one of you can help me in 
suggesting a company or person whom I can talk about the issue or some one who 
can come and look at the  instrument and suggest the best advice . I assume 
probably some electric motor is dead inside. 
Any advice will be really helpful.
Thank you,
Lakshmi,
Sr. research assistant,
Pathology core Lab,
George Washington University,
Washington, DC.

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[Histonet] Cryostat repair

[Michael J. Lyon, Ph.D.]

We have a:

Reichert HistoSTAT 

975CJ cryostat microtome 

model: 855 

serial #: 0819-3410

 

That needs attention.  Any suggestions?

 

Thanks

 

 

Michael J. Lyon, PhD

Otolaryngology Research Labs

SUNY Upstate Medical University

750 East Adams Street

Syracuse, NY 13210

 

Voice: 315-464-7253

Fax: 315-464-5572

 

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[Histonet] Cryostat sectioning

[Chen, Shu-Cheng]

Hi,

We are searching for a fee for service lab experienced in cryostat sectioning 
of animal and human tissues. Any recommendation is appreciated.

Thanks,
Shu-Cheng

Notice:  This e-mail message, together with any attachments, contains
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[Histonet] Cryostat

[Rolly Perez]

Anyone interested in a used, working Cryostat, contact me directly:

Leica/Reichert-Jung CRYOCUT 1800

Rolly Perez, HT(ASCP)
Histology Supervisor
MedSurg Pathology Associates, Inc.
Tigard, OR
503.443.2157




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[Histonet] Cryostat cutting?

[Francis OBrien]

Hello,

We need to cut clean and consistent 50 micron sections of OCT embedded
tissue on our leica cryostat (CM-1850). Does anyone on the list have
any tips or tricks for doing this when compared to cutting the usual
10 micron sections that we usually deal with in our lab.

-- 
Regards,

Francis

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[Histonet] Cryostat Maintenance and Repair - CA

[Eric Velazquez]

Hi,

Can somebody please recommend to me a company that does cryostat maintenance
and repair? I am looking for a company that is familiar with both Micron and
Leica models in the Irvine, CA area.

Thanks,
Eric
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RE: [Histonet] Cryostat Maintenance and Repair - CA

[Laurie Colbert]

Hi Eric,

Mikron Instruments services most of our instruments, including Micron
brand.  I don't have any Leica equipment now, but they have serviced my
Leica microtome in the past.  They are very good!  The service manager's
name is Steve Pike.  Mikron is in Vista, CA and their phone number is
(800) 377-5395.

Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric
Velazquez
Sent: Wednesday, May 04, 2011 2:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Maintenance and Repair - CA

Hi,

Can somebody please recommend to me a company that does cryostat
maintenance
and repair? I am looking for a company that is familiar with both Micron
and
Leica models in the Irvine, CA area.

Thanks,
Eric
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Re: [Histonet] cryostat repair--thanks!

[Emily Sours]

The compressor fan was loose, so when the Tech One guy fixed it, instead of
making a horrible noise when it turns off, it just rattles.  Oh well, time
for a new motor.
Which leads me to another question, is there any reason I should call Lecia
to replace this motor? The guy from Tech One Biomedical seemed to know his
stuff, but my boss seems to think we should call the people who made the
cryostat.  Personally, I think that would be spending a lot more money for
the same thing, but maybe I'm missing something.  Any suggestions?
By the way, the cryostat is from 2008 and isn't under warranty.

Emily


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Fri, Apr 1, 2011 at 3:15 PM, Victor Tobias 
vic...@pathology.washington.edu wrote:

 So what was the problem?

 Victor Tobias HT(ASCP)
 Clinical Applications Analyst
 University of Washington Medical Center
 Dept of Pathology Room BB220
 1959 NE Pacific
 Seattle, WA 98195
 vic...@pathology.washington.edu
 206-744-2735
 206-744-8240 Fax
 =
 Privileged, confidential or patient identifiable information may be
 contained in this message. This information is meant only for the use
 of the intended recipients. If you are not the intended recipient, or
 if the message has been addressed to you in error, do not read,
 disclose, reproduce, distribute, disseminate or otherwise use this
 transmission. Instead, please notify the sender by reply e-mail, and
 then destroy all copies of the message and any attachments.



 On 4/1/2011 12:09 PM, Emily Sours wrote:

 Thanks to all who answered my email, I ended up calling Tech One
 Biomedical
 and they came right out.  I recommend them to anyone who needs histology
 equipment repaired!!

 Emily
 ps. this is not an april fool's joke.
 pps. really!

 It has become almost a cliche to remark that nobody boasts of ignorance of
 literature, but it is socially acceptable to boast ignorance of science
 and
 proudly claim incompetence in mathematics.
 -Richard Dawkins
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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RE: [Histonet] cryostat repair--thanks!

[Weems, Joyce]

The Tech One guy would do the same thing for much cheaper. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, April 05, 2011 10:11
To: Victor Tobias; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryostat repair--thanks!

The compressor fan was loose, so when the Tech One guy fixed it, instead of 
making a horrible noise when it turns off, it just rattles.  Oh well, time for 
a new motor.
Which leads me to another question, is there any reason I should call Lecia to 
replace this motor? The guy from Tech One Biomedical seemed to know his stuff, 
but my boss seems to think we should call the people who made the cryostat.  
Personally, I think that would be spending a lot more money for the same thing, 
but maybe I'm missing something.  Any suggestions?
By the way, the cryostat is from 2008 and isn't under warranty.

Emily


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Fri, Apr 1, 2011 at 3:15 PM, Victor Tobias  
vic...@pathology.washington.edu wrote:

 So what was the problem?

 Victor Tobias HT(ASCP)
 Clinical Applications Analyst
 University of Washington Medical Center Dept of Pathology Room BB220
 1959 NE Pacific
 Seattle, WA 98195
 vic...@pathology.washington.edu
 206-744-2735
 206-744-8240 Fax
 =
 Privileged, confidential or patient identifiable information may be 
 contained in this message. This information is meant only for the use 
 of the intended recipients. If you are not the intended recipient, or 
 if the message has been addressed to you in error, do not read, 
 disclose, reproduce, distribute, disseminate or otherwise use this 
 transmission. Instead, please notify the sender by reply e-mail, and 
 then destroy all copies of the message and any attachments.



 On 4/1/2011 12:09 PM, Emily Sours wrote:

 Thanks to all who answered my email, I ended up calling Tech One 
 Biomedical and they came right out.  I recommend them to anyone who 
 needs histology equipment repaired!!

 Emily
 ps. this is not an april fool's joke.
 pps. really!

 It has become almost a cliche to remark that nobody boasts of 
 ignorance of literature, but it is socially acceptable to boast 
 ignorance of science and proudly claim incompetence in mathematics.
 -Richard Dawkins
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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It may contain information that is privileged and 
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not the intended recipient, please delete this message, and 
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[Histonet] cryostat repair--thanks!

[Emily Sours]

Thanks to all who answered my email, I ended up calling Tech One Biomedical
and they came right out.  I recommend them to anyone who needs histology
equipment repaired!!

Emily
ps. this is not an april fool's joke.
pps. really!

It has become almost a cliche to remark that nobody boasts of ignorance of
literature, but it is socially acceptable to boast ignorance of science and
proudly claim incompetence in mathematics.
-Richard Dawkins
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[Histonet] cryostat repair

[Emily Sours]

Our cryostat (CM3050S) is making a terrible noise when the fan stops
running, yet I don't want to call Leica (the manufacturer) because they
charge more money than anyone could ever afford.  How would I go about
finding someone who could repair a cryostat in Pittsburgh? I don't think
it's the refrigeration that's broken, it sounds like something mechanical.
Any suggestions?
Has anyone ever tried to open the back of one to see if it's fixable? I'm no
mechanic, but I think I can spot a worn belt or loose screw.  I'm just not
sure I want to open it up myself.

Emily

It has become almost a cliche to remark that nobody boasts of ignorance of
literature, but it is socially acceptable to boast ignorance of science and
proudly claim incompetence in mathematics.
-Richard Dawkins
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RE: [Histonet] cryostat repair

[DANIEL HEWITT]

Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars
and do most of our repairs. Ray does an excellent job at a good price.
It sounds like the bearings in your fan or maybe the compressor.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
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is prohibited.  If you are not the intended recipient, or an agent
responsible for delivering the message to the intended recipient, please
contact the sender by reply e-mail and delete and destroy all copies of
the original message, including attachments.

Please note that any views or opinions presented in this e-mail are
solely those of the author and do not necessarily represent those of
Heritage Valley Health System.  The integrity and security of this
message cannot be guaranteed on the internet.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Thursday, March 31, 2011 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat repair

Our cryostat (CM3050S) is making a terrible noise when the fan stops
running, yet I don't want to call Leica (the manufacturer) because they
charge more money than anyone could ever afford.  How would I go about
finding someone who could repair a cryostat in Pittsburgh? I don't think
it's the refrigeration that's broken, it sounds like something
mechanical.
Any suggestions?
Has anyone ever tried to open the back of one to see if it's fixable?
I'm no
mechanic, but I think I can spot a worn belt or loose screw.  I'm just
not
sure I want to open it up myself.

Emily

It has become almost a cliche to remark that nobody boasts of ignorance
of
literature, but it is socially acceptable to boast ignorance of science
and
proudly claim incompetence in mathematics.
-Richard Dawkins
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RE: [Histonet] cryostat repair

[Bernice Frederick]

We use them too- Illinois office.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bouchal,
Rena L
Sent: Thursday, March 31, 2011 1:38 PM
To: Emily Sours; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat repair

We are using Tech One Biomedical... service reps in Pghfor almost all of
our routine instrument service really good and half the price of the
vendors!  Their number is 866-497-3033 or www.techoneweb.com

Please note that my email address  as of Jan 3, 2011 is
bouch...@wvuhealthcare.com  .   Please make the appropriate changes in
your address book.

Rena Bouchal, M.S.
Anatomic Pathology Manager
West Virginia University Hospitals
304-293-7765

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DANIEL
HEWITT
Sent: Thursday, March 31, 2011 2:15 PM
To: Emily Sours; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat repair

Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and
do most of our repairs. Ray does an excellent job at a good price.
It sounds like the bearings in your fan or maybe the compressor.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

This email, including any attachments, is for the sole use of the intended
recipient(s) and may contain confidential and privileged information.  Any
unauthorized review, use, disclosure or distribution is prohibited.  If you
are not the intended recipient, or an agent responsible for delivering the
message to the intended recipient, please contact the sender by reply e-mail
and delete and destroy all copies of the original message, including
attachments.

Please note that any views or opinions presented in this e-mail are solely
those of the author and do not necessarily represent those of Heritage
Valley Health System.  The integrity and security of this message cannot be
guaranteed on the internet.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Thursday, March 31, 2011 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat repair

Our cryostat (CM3050S) is making a terrible noise when the fan stops
running, yet I don't want to call Leica (the manufacturer) because they
charge more money than anyone could ever afford.  How would I go about
finding someone who could repair a cryostat in Pittsburgh? I don't think
it's the refrigeration that's broken, it sounds like something mechanical.
Any suggestions?
Has anyone ever tried to open the back of one to see if it's fixable?
I'm no
mechanic, but I think I can spot a worn belt or loose screw.  I'm just not
sure I want to open it up myself.

Emily

It has become almost a cliche to remark that nobody boasts of ignorance of
literature, but it is socially acceptable to boast ignorance of science and
proudly claim incompetence in mathematics.
-Richard Dawkins
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and privileged information.  Any unauthorized review, use, disclosure or
distribution is prohibited.
 If you are not the intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message.

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[Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

[Gladney, Diane C Ms CIV USA MEDCOM MACH]

Classification:  UNCLASSIFIED
Caveats: NONE

Dear Histonetters,

Our 11 year old cryostat is beginning to experience some unusual problems. Our 
Medical Maintenance says that they won't put a lot of money into fixing it 
because of the age of the unit. Therefore, I am asking for opinions, pros, cons 
of different brands of cryostats. I am interested in a cryostat that has the UV 
light or other source of easy decontamination. We don't do a lot of frozen 
sections but enough for us to have to have a good cryostat that is easy to 
operate and clean.  Any comments or suggestions would be greatly appreciated.

Thanks,
Diane Gladney


Diane C. Gladney, HT (ASCP)
Histology Supervisor
Department of Pathology
Moncrief Army Community Hospital
4500 Stuart Street
FT. Jackson, SC  29207

Phone:  803-751-2530
Fax:  803-751-7829




Classification:  UNCLASSIFIED
Caveats: NONE

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Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

[Phyllis Thaxton]

Hi Diane,
  We have been happy with the Leica cryostats over the years. We have an 1800 
that is about 15 years old, an 1850 that is about 5 years old with the UV light 
for decontamination.
 Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 





From: Gladney, Diane C Ms CIV USA MEDCOM MACH diane.glad...@us.army.mil
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Wed, March 30, 2011 7:50:23 AM
Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

Classification:  UNCLASSIFIED
Caveats: NONE

Dear Histonetters,

Our 11 year old cryostat is beginning to experience some unusual problems. Our 
Medical Maintenance says that they won't put a lot of money into fixing it 
because of the age of the unit. Therefore, I am asking for opinions, pros, cons 
of different brands of cryostats. I am interested in a cryostat that has the UV 
light or other source of easy decontamination. We don't do a lot of frozen 
sections but enough for us to have to have a good cryostat that is easy to 
operate and clean.  Any comments or suggestions would be greatly appreciated.

Thanks,
Diane Gladney


Diane C. Gladney, HT (ASCP)
Histology Supervisor
Department of Pathology
Moncrief Army Community Hospital
4500 Stuart Street
FT. Jackson, SC  29207

Phone:  803-751-2530
Fax:  803-751-7829




Classification:  UNCLASSIFIED
Caveats: NONE

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RE: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)

[Sheila Adey]

Hi Diane,
We just purchased a cryostat from Sakura, we love it. Great features. 
 
 Date: Wed, 30 Mar 2011 06:38:41 -0700
 From: dch...@yahoo.com
 To: diane.glad...@us.army.mil; histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
 CC: 
 
 Hi Diane,
   We have been happy with the Leica cryostats over the years. We have an 1800 
 that is about 15 years old, an 1850 that is about 5 years old with the UV 
 light 
 for decontamination.
  Phyllis Thaxton HT(ASCP)QIHC
 DCH Regional Medical Center
 Tuscaloosa, AL 
 
 
 
 
 
 From: Gladney, Diane C Ms CIV USA MEDCOM MACH diane.glad...@us.army.mil
 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
 Sent: Wed, March 30, 2011 7:50:23 AM
 Subject: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
 
 Classification:  UNCLASSIFIED
 Caveats: NONE
 
 Dear Histonetters,
 
 Our 11 year old cryostat is beginning to experience some unusual problems. 
 Our 
 Medical Maintenance says that they won't put a lot of money into fixing it 
 because of the age of the unit. Therefore, I am asking for opinions, pros, 
 cons 
 of different brands of cryostats. I am interested in a cryostat that has the 
 UV 
 light or other source of easy decontamination. We don't do a lot of frozen 
 sections but enough for us to have to have a good cryostat that is easy to 
 operate and clean.  Any comments or suggestions would be greatly appreciated.
 
 Thanks,
 Diane Gladney
 
 
 Diane C. Gladney, HT (ASCP)
 Histology Supervisor
 Department of Pathology
 Moncrief Army Community Hospital
 4500 Stuart Street
 FT. Jackson, SC  29207
 
 Phone:  803-751-2530
 Fax:  803-751-7829
 
 
 
 
 Classification:  UNCLASSIFIED
 Caveats: NONE
 
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[Histonet] Cryostat CM 3050

[bce_services]

Hi all,
 I'm looking for an old Leica CM3050 cryostat if anyone has a broken or 
mothballed one they want to sell for parts, Thanking you in advance 
Brian
B A Cornett-Earley
BCE Services,LLC
Web: bceservicesllc.com
Tel 770-329-6370
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[Histonet] Cryostat help

[Blundon, Kimberly]

Hello Histonetters,

 

I am new to the histology world and I was hoping to get some feedback
about staining after cutting sections in the cryostat. 

 

Does anyone have a protocol they use for staining after sectioning
tissue in a cryostat?

 

I have never done it before so I thought I would come to the experts.

 

Thanks for your help!

 

Kimberly

 

 

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Re: [Histonet] Cryostat help

[Rene J Buesa]

That depends on what staining you need to use.
If an IHC, just fix the sections in acetone, air dry and proceed without HIER
If HE fix quickly with formalin 10%, wash → hematoxylin for 30 secs without 
differentiation → eosin 15 sec→ dehydrate and mount.
Other HC procedures all will require fixation and using the specific protocol.
Always remember that it is a frozen section and therefore more delicate and 
less adhered than a paraffin section.
René J.

--- On Tue, 1/11/11, Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca wrote:


From: Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca
Subject: [Histonet] Cryostat help
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, January 11, 2011, 10:28 AM


Hello Histonetters,



I am new to the histology world and I was hoping to get some feedback
about staining after cutting sections in the cryostat. 



Does anyone have a protocol they use for staining after sectioning
tissue in a cryostat?



I have never done it before so I thought I would come to the experts.



Thanks for your help!



Kimberly





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RE: [Histonet] Cryostat help

[Setlak, Lisa]

We have a similar frozen section protocol but we fix the slide in 95% alcohol 
for ~1minute and then stain with Gill's Hematoxylin for 1 minute, rinse, blue 
and then alcohol, foloowed by Eosin for ~ 15secs.- dehydrate and mount.
Lisa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, January 11, 2011 10:29 AM
To: histonet@lists.utsouthwestern.edu; KimberlyBlundon
Subject: Re: [Histonet] Cryostat help

That depends on what staining you need to use.
If an IHC, just fix the sections in acetone, air dry and proceed without HIER
If HE fix quickly with formalin 10%, wash → hematoxylin for 30 secs without 
differentiation → eosin 15 sec→ dehydrate and mount.
Other HC procedures all will require fixation and using the specific protocol.
Always remember that it is a frozen section and therefore more delicate and 
less adhered than a paraffin section.
René J.

--- On Tue, 1/11/11, Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca wrote:


From: Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca
Subject: [Histonet] Cryostat help
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, January 11, 2011, 10:28 AM


Hello Histonetters,



I am new to the histology world and I was hoping to get some feedback
about staining after cutting sections in the cryostat. 



Does anyone have a protocol they use for staining after sectioning
tissue in a cryostat?



I have never done it before so I thought I would come to the experts.



Thanks for your help!



Kimberly





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RE: [Histonet] Cryostat Question Please

[Sherwood, Margaret]

We have this model as well and like it.  We got the vacuum attachment.  It's not
perfect, but does facilitate cleaning up OCT and tissue scraps.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Wednesday, November 24, 2010 4:14 PM
To: Paula Lucas; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cryostat Question Please

Very consistant, easy to use. 

2 drawbacks  

1. We got the decontamination add-on trying to automate the CAP
requirement, but now it seems Thermo says it is not an adequate process.
I believe they are doing more intense studies, but for the time being, I
have to decontaminate it by hand. 

2. OCT and tissue scraps build up under collection device and cause the
mechanism to clunk unless buildup is removed. 

These two things add a good chunk of time to my day.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Wednesday, November 24, 2010 1:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Question Please

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula
Lab Manager
BP Medical Group
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[Histonet] Cryostat Question Please

[Paula Lucas]

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula 
Lab Manager
BP Medical Group
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RE: [Histonet] Cryostat Question Please

[O'Donnell, Bill]

Very consistant, easy to use. 

2 drawbacks  

1. We got the decontamination add-on trying to automate the CAP
requirement, but now it seems Thermo says it is not an adequate process.
I believe they are doing more intense studies, but for the time being, I
have to decontaminate it by hand. 

2. OCT and tissue scraps build up under collection device and cause the
mechanism to clunk unless buildup is removed. 

These two things add a good chunk of time to my day.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Lucas
Sent: Wednesday, November 24, 2010 1:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Question Please

Hello all
 
Has anyone used a Richard-Allan/Thermo-Fisher HM550 MP cryostat?  If so,
what is your opinion/pros and cons?
 
Thanks so much in advance,
Paula
Lab Manager
BP Medical Group
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Re: [Histonet] cryostat question

[Alan Bright]

Dear Susan,
Our Starlet 2212 Bench Top Cryostat has an option for orientation. There are 
many used for Mohs, as holding a low temperature is not a problem. I can email 
you some user references  if. You want.
Regards: Alan Bright
Bright Instrument Co. Ltd.
England 
Sent from my BlackBerry® wireless device

-Original Message-
From: histo...@aol.com
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Fri, 29 Oct 2010 22:10:21 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat question


Does anyone have 'hands on'  experience performing Mohs using a countertop 
cryostat?  Can the specimen head be manipulated to acccomodate a badly embedded 
block? Does the machine stay cold enough to perform Mohs? Any advice would be 
appreciated.  
My e-mail is histo...@aol.com. Thanks, Susan
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[Histonet] cryostat question

[historsd]

Does anyone have 'hands on'  experience performing Mohs using a countertop 
cryostat?  Can the specimen head be manipulated to acccomodate a badly embedded 
block? Does the machine stay cold enough to perform Mohs? Any advice would be 
appreciated.  
My e-mail is histo...@aol.com. Thanks, Susan
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[Histonet] Cryostat correction

[Andrew Burgeson]

lol...At the end of my last post I meant to say, I DO NOT
think he knows what he is doing!

oops

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[Histonet] Cryostat decontamination

[thisisann]

Can someone send me a procedure on how to decontaminate a cryostat?  Thank you,

Ann



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RE: [Histonet] cryostat

[histotech]

Jeff,

We have both the Leica CM1850 and the CM1850 UV. These are not top of the
line models, but they work very well for us.  You can find out more
information on the unit at the following URL:

http://www.leica-microsystems.com/products/histology-systems/sectioning/cryo
stats/details/product/leica-cm1850-uv/


Michelle

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey
Thompson
Sent: Friday, August 20, 2010 5:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat


Hello,
 
I am writing to ask about what the community views are on the best -or worst
- cryostats. We may have the opportunity to purchase one for the first time
in many years and we would like to get a good, solid, reliable model. We're
not really looking for one (and can't really afford one) with all the bells
and whistles, but would like to know what folks think about the ones to look
for as well as the ones to steer clear of.
 
All input is appreciated.
 
Thanks,
 
Jeff Thompson  
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RE: [Histonet] cryostat

[histotech]

Jeff, please note that the URL posted below wrapped into two lines.

Michelle



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Sunday, August 22, 2010 12:11 PM
To: 'Jeffrey Thompson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat


Jeff,

We have both the Leica CM1850 and the CM1850 UV. These are not top of the
line models, but they work very well for us.  You can find out more
information on the unit at the following URL:

http://www.leica-microsystems.com/products/histology-systems/sectioning/cryo
stats/details/product/leica-cm1850-uv/


Michelle

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey
Thompson
Sent: Friday, August 20, 2010 5:55 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat


Hello,
 
I am writing to ask about what the community views are on the best -or worst
- cryostats. We may have the opportunity to purchase one for the first time
in many years and we would like to get a good, solid, reliable model. We're
not really looking for one (and can't really afford one) with all the bells
and whistles, but would like to know what folks think about the ones to look
for as well as the ones to steer clear of.
 
All input is appreciated.
 
Thanks,
 
Jeff Thompson  
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Checked by AVG - www.avg.com 
Version: 8.5.441 / Virus Database: 271.1.1/3071 - Release Date: 08/15/10
18:35:00


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Re: [Histonet] cryostat cutting problems

[Emily Sours]

It may help to let your block equilibrate for about 20 minutes in the
cryostat.  I've found blocks do not section well if you start sectioning
right away, especially snap frozen ones.  Also, do you embed in OCT/sucrose?
A 1:1 OCT: 30% sucrose solution is much softer than just OCT (or whatever
embedding medium you are using).
What do you mean by the tissue is condensed? It's folding up? I've found
sectioning fresh unfixed tissue on a cryostat is impossible (we tried chick
embryo trunks, which may be harder to section fresh than other tissue)--it
doesn't section but get smushed to a pulp.  You need to use a sledge
microtome, unfortunately.
What thickness are you sectioning at? I've found sectioning above sixty
microns doesn't work as well on a cryostat, but again this is on fixed chick
embryo tissue.
If your cryostat is old, I would suggest you look for a new anti-roll plate
made of glass.  They work so much better.  As long as your cryostat isn't
older than about ten years, you should be able to find one that fits your
knife holder very easily.
That said, I've used a Rei-something cryostat that was 15 years old and it
sucked.  The anti-roll plate holder was too loose to do anything useful and
the adjustment of the blade holder was impossible.  Can you use a newer
cryostat in your department from another lab? Or just go back to sledge
microtome sectioning--if you know how to do it, it's the same principle
anyway.

Emily
--
Outside of a dog, a book is man's best friend. Inside of a dog it's too dark
to read.
--Groucho Marx


On Fri, Aug 13, 2010 at 4:53 PM, Entwistle, Laura lentwis...@ucsd.eduwrote:

 I am new to using a cryostat and am having some issues with my tissue.


 1.Every slice seems to shatter and fragment.  When I have used a
 microtome it was because the tissue was too cold.

 2.   The tissue itself was flash frozen and so was not fixed and the
 tissue did not go through the perfusion process.

 3.   Each slice is condensed and doesn't lay flat.

 4.   The cryostat is old.

 Any tips would be very helpful.
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[Histonet] cryostat cutting problems

[Entwistle, Laura]

I am new to using a cryostat and am having some issues with my tissue.


1.Every slice seems to shatter and fragment.  When I have used a 
microtome it was because the tissue was too cold.

2.   The tissue itself was flash frozen and so was not fixed and the tissue 
did not go through the perfusion process.

3.   Each slice is condensed and doesn't lay flat.

4.   The cryostat is old.

Any tips would be very helpful.
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[Histonet] Cryostat Decontamination

[Myers, Karyn]

Hello,
Our Hospital has several Frozen Section Rooms, each of which have 1 to 2 
cryostats.
The majority of our cryostats are the newer models, with the built in 
Ultraviolet Radiation Disinfection cycle (runs every afternoon.)  However, we 
still have a couple of the older models that do not have an automatic 
disinfection cycle.What are other institutions currently doing to disinfect 
their cryostats.
Any information/policy will be greatly appreciated,
Karyn




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[Histonet] Cryostat Decontamination

[malbena...@gmail.com]

Hy Karyrn.

As my lab carry frozen section daily, the decontamination/fumigation of the
cryostat on a daily basis is not practical as we do not have a back up
cryostat. Also after every frozen section, we make sure that the Cryostat is
get disinfected of any fresh tissue trimming with 70 % alcohol.
Every friday night the cryostat get set-up for fumigation and get
decontaminated weekly using Formaldehyde vapour/ then we neutralise them
with ammonia, according to manufacturer recommendation (Thermo Scientific),
though the fumigation will not activate until Sunday night so Week-end
On-Call staff can carry urgent Frozen sections if needed.  Each
decontamination/fumigation cycle is then Log, for CPA purposes.

Hope this helps

Malika



-- Forwarded message --
From: Myers, Karyn karynmy...@texashealth.org
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu

Date: Wed, 21 Jul 2010 08:57:35 -0500
Subject: [Histonet] Cryostat Decontamination

Hello,
Our Hospital has several Frozen Section Rooms, each of which have 1 to 2
cryostats.
The majority of our cryostats are the newer models, with the built in
Ultraviolet Radiation Disinfection cycle (runs every afternoon.)  However,
we still have a couple of the older models that do not have an automatic
disinfection cycle.What are other institutions currently doing to
disinfect their cryostats.
Any information/policy will be greatly appreciated,
Karyn




The information contained in this message and any attachments is intended
only for the use of the individual or entity to which it is addressed, and
may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from
disclosure under applicable law.  If you are not the intended recipient, you
are prohibited from copying, distributing, or using the information.  Please
contact the sender immediately by return e-mail and delete the original
message from your system.

-- 
 Smile  it confuses people  
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[Histonet] Cryostat vs decal sections of bone

[louise renton]

Hi all,

last year sometime I was asked to budget for some equipment for our unit.
Not expecting to get anything i aimed high, and requested a cryostat and
cryoJane system.
Lo and behold, the planetary influences were just right, and my request was
approved. Now, I've got cold feet (pardon the pun) and I'm wondering if
there *are*  any distinct advantages of cryosections of bone over
demineralised wax embedded samples in regards to immuno and in-situ ?.

-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel  fax)
073 5574456 (emergencies only)
There are nights when the wolves are silent and only the moon howls.
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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