Re: [Histonet] Microtome block holder

[Colleen Forster via Histonet]

You are very welcome!

Colleen

On Tue, Nov 7, 2023 at 4:02 PM Naira Margaryan 
wrote:

> Awesome, thank you so much Colleen, for such quick response,
> Naira
>
> On Tue, Nov 7, 2023 at 4:01 PM Colleen Forster  wrote:
>
>> Naira,
>>
>> Contact Lee Dickey with Ted Pella. They carry the whole line of large
>> block adapters for the Leica microtomes.
>>
>> lee_dic...@tedpella.com
>>
>> I have this set up and it works great.
>>
>> Colleen Forster HT(ASCP)QIHC
>>
>> On Tue, Nov 7, 2023 at 3:58 PM Naira Margaryan via Histonet <
>> histonet@lists.utsouthwestern.edu> wrote:
>>
>>> Hello,
>>>
>>> We are thinking to process the dog stifle joints. For that reason we will
>>> need large cassette/slides (3x2) and more importantly to find a large
>>> block
>>> holder for the Leica Microtome.
>>>
>>> Any advice and suggestions where to purchase all large parts would be
>>> appreciated.
>>>
>>> Thanks in advance,
>>> Naira
>>>
>> ___
>>> Histonet mailing list
>>> Histonet@lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>>
>>
>> --
>> Colleen Forster HT(ASCP)QIHC
>> BLS Histology and IHC Laboratory
>> Jackson Hall, Room 2-155
>> 321 Church St. SE
>> 
>> Minneapolis, MN 55455
>> 
>> 612-626-1930
>>
>>
>>
>>
>>
>>
>>

-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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Re: [Histonet] Microtome block holder

[Naira Margaryan via Histonet]

Awesome, thank you so much Colleen, for such quick response,
Naira

On Tue, Nov 7, 2023 at 4:01 PM Colleen Forster  wrote:

> Naira,
>
> Contact Lee Dickey with Ted Pella. They carry the whole line of large
> block adapters for the Leica microtomes.
>
> lee_dic...@tedpella.com
>
> I have this set up and it works great.
>
> Colleen Forster HT(ASCP)QIHC
>
> On Tue, Nov 7, 2023 at 3:58 PM Naira Margaryan via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>> Hello,
>>
>> We are thinking to process the dog stifle joints. For that reason we will
>> need large cassette/slides (3x2) and more importantly to find a large
>> block
>> holder for the Leica Microtome.
>>
>> Any advice and suggestions where to purchase all large parts would be
>> appreciated.
>>
>> Thanks in advance,
>> Naira
>>
> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>>
>
> --
> Colleen Forster HT(ASCP)QIHC
> BLS Histology and IHC Laboratory
> Jackson Hall, Room 2-155
> 321 Church St. SE
> 
> Minneapolis, MN 55455
> 
> 612-626-1930
>
>
>
>
>
>
>
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Re: [Histonet] Microtome block holder

[Colleen Forster via Histonet]

Naira,

Contact Lee Dickey with Ted Pella. They carry the whole line of large block
adapters for the Leica microtomes.

lee_dic...@tedpella.com

I have this set up and it works great.

Colleen Forster HT(ASCP)QIHC

On Tue, Nov 7, 2023 at 3:58 PM Naira Margaryan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello,
>
> We are thinking to process the dog stifle joints. For that reason we will
> need large cassette/slides (3x2) and more importantly to find a large block
> holder for the Leica Microtome.
>
> Any advice and suggestions where to purchase all large parts would be
> appreciated.
>
> Thanks in advance,
> Naira
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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[Histonet] Microtome block holder

[Naira Margaryan via Histonet]

Hello,

We are thinking to process the dog stifle joints. For that reason we will
need large cassette/slides (3x2) and more importantly to find a large block
holder for the Leica Microtome.

Any advice and suggestions where to purchase all large parts would be
appreciated.

Thanks in advance,
Naira
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Re: [Histonet] microtome pm

[Travis via Histonet]

Depends on the make and model, same way a classic car would need more upkeep
compared to 2022 model that is still under manufacturer warranty. 6 months
for an old microtome might be necessary 

Travis O’Brien
 
International Medical Equipment, Inc.
170 Vallecitos De Oro
San Marcos, CA 92069
 
800.543.8496  Phone
760.761.0859  Fax
www.imebinc.com

-Original Message-
From: Paula via Histonet  
Sent: Tuesday, June 28, 2022 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome pm

Hi everyone,

 

How often do you PM your microtomes?  Currently, we have them done every 6
months but I'm thinking once a year is sufficient and I wanted to get some
input before we decide.

 

Thank you in advance,

Paula

Bio-Path Medical Group

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Re: [Histonet] microtome pm

[Cooper, Brian via Histonet]

Once a year is good for us.  Just an added thought: we always make sure they 
change the extension springs in the cassette clamp with each PM as well.  

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Paula via Histonet  
Sent: Tuesday, June 28, 2022 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome pm (EXTERNAL EMAIL)

CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside 
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Hi everyone,

 

How often do you PM your microtomes?  Currently, we have them done every 6 
months but I'm thinking once a year is sufficient and I wanted to get some 
input before we decide.

 

Thank you in advance,

Paula

Bio-Path Medical Group

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[Histonet] microtome pm

[Paula via Histonet]

Hi everyone,

 

How often do you PM your microtomes?  Currently, we have them done every 6
months but I'm thinking once a year is sufficient and I wanted to get some
input before we decide.

 

Thank you in advance,

Paula

Bio-Path Medical Group

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Re: [Histonet] microtome blades and wax

[Rathborne, Toni via Histonet]

Jessica,

What has Leica said about the problem? I have always found them to be 
responsive and able to provide suggestions. This would be especially true with 
a new instrument, still under warranty. We have never had any inconsistencies 
with our Autocut, which was purchased last spring.

Toni




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and destroy any printouts.

-Original Message-
From: Piche, Jessica via Histonet 
Sent: Friday, January 14, 2022 8:43 AM
To: Histonet 
Subject: [Histonet] microtome blades and wax


*** This is an External Email ***

Good Morning,

We have been using Leica Histocore Autocut microtomes for a few months and have 
been having inconsistency with cutting on them. We have tried so many different 
blades, high and low, and have tried changing the blade angle. Nothing is a 
perfect fix, and we feel like we are really having to work much harder to get a 
great section. We were wondering what everyone else uses. I was also wondering 
if the wax could be an issue. We use EM-400 for embedding. I'd appreciate 
anyone's thoughts.

Thank you and have a nice weekend!

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
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Re: [Histonet] microtome blades and wax

[P Sicurello via Histonet]

Hi Jessica,

Happy Friday. 

Do you use the same EM-400 paraffin for processing?  If you use different 
paraffins that might contribute to your sectioning problems.   

If the waxes are different the tissue and the rest of the block are not one 
continuous unit in the "eyes" of the knife. The waxes have different properties 
so they cut differently because the embedding wax does not infiltrate into the 
tissue but wraps around it like a blanket.  

I'm not sure if that's what your lab does but if it is I suggest using the same 
paraffin for processing and embedding. 

Sincerely,

Paula Sicurello

Sent from my iPhone

> On Jan 14, 2022, at 5:43 AM, Piche, Jessica via Histonet 
>  wrote:
> 
> Good Morning,
> 
> We have been using Leica Histocore Autocut microtomes for a few months and 
> have been having inconsistency with cutting on them. We have tried so many 
> different blades, high and low, and have tried changing the blade angle. 
> Nothing is a perfect fix, and we feel like we are really having to work much 
> harder to get a great section. We were wondering what everyone else uses. I 
> was also wondering if the wax could be an issue. We use EM-400 for embedding. 
> I'd appreciate anyone's thoughts.
> 
> Thank you and have a nice weekend!
> 
> Jessica
> 
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory
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[Histonet] microtome blades and wax

[Piche, Jessica via Histonet]

Good Morning,

We have been using Leica Histocore Autocut microtomes for a few months and have 
been having inconsistency with cutting on them. We have tried so many different 
blades, high and low, and have tried changing the blade angle. Nothing is a 
perfect fix, and we feel like we are really having to work much harder to get a 
great section. We were wondering what everyone else uses. I was also wondering 
if the wax could be an issue. We use EM-400 for embedding. I'd appreciate 
anyone's thoughts.

Thank you and have a nice weekend!

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
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[Histonet] microtome

[Silvia Bonner via Histonet]

Hello,

We are in the market for a new/refurbished microtome.  We currently have a 
Micron HM355s, and Micron HM 335E.  We are interested in buying something 
comparable.  Any suggestions?  We are looking at Micron and Leica.  Good or bad 
reviews would be appreciated.

Thanks,


Silvia Bonner, BS, HT(ASCP) CM

Histology Supervisor



sbon...@pathregional.com

Pathologists’ Regional Laboratory

Anatomical Pathology

2841 Juniper Dr. Suite 1

Lewiston, ID 83501

(208)746-5533



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Re: [Histonet] microtome part

[LEROY H BROWN via Histonet]

Anyone happen to have a spare low profile knife holder laying around?  

Looking for one to fit my MICROM MODEL HM 330.  

Happy to pay you.   It is for my histo tech.  She needs a replacement for
her microtome.

 

Thanks,

LeRoy Brown HT(ASCP) HTL

HCS

 

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Re: [Histonet] Microtome Recommendations

[Bacon, Charles via Histonet]

Hi Kelly,

We have tried several manufactures, as well as model types over the years. We 
have had sliding microtomes (Microm, no longer available), and rotary 
microtomes from, Leizt (old), AO (very old), and Leica. We have had several 
models from Leica and prefer their durability. If you are manual, you are hard 
pressed to find a more durable and reliable microtome than the "Leica RM2125 
RTS - The Essential Microtome" you can get the gross feed wheel in "American 
(toward you)" or "European (away from you)" so be careful to ask when ordering. 
If you are looking for Automatic we have 10 of the  RM2255 from Leica, which I 
think is now called "HistoCore AUTOCUT - Automated Rotary Microtome." These are 
great and several of my techs have adopted the use of a foot pedal to preserve 
their shoulders.  Good luck and happy shopping!

Best,

Chuck Bacon, HTL(ASCP)CM 
Supervisor Histology
Baystate Medical Center
361 Whitney Ave., Holyoke, MA 01040
Telephone: 413-322-4786  Fax: 413-322-4790
charles.ba...@baystatehealth.org

-Original Message-
From: Pairan, Kelly  
Sent: Tuesday, February 09, 2021 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome Recommendations

Good Morning Histoland,
We're looking into requesting capital for a new microtome and we were wondering 
what other labs were using and would recommend.

Thanks for your feedback!
Kelly

Kelly Pairan,  BS, HT (ASCP)CM, QIHC (ASCP)
Histology Supervisor-Anatomic Pathology
Department of Pathology and Laboratory Medicine
Email:  kelly.pai...@nationwidechildrens.org
ph: 614-722-5414
fx: 614-722-3033



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[Histonet] Microtome Recommendations

[Pairan, Kelly via Histonet]

Good Morning Histoland,
We're looking into requesting capital for a new microtome and we were wondering 
what other labs were using and would recommend.

Thanks for your feedback!
Kelly

Kelly Pairan,  BS, HT (ASCP)CM, QIHC (ASCP)
Histology Supervisor-Anatomic Pathology
Department of Pathology and Laboratory Medicine
Email:  kelly.pai...@nationwidechildrens.org
ph: 614-722-5414
fx: 614-722-3033

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[Histonet] Microtome

[Silvia Bonner via Histonet]

Hi Everyone,

We are looking to purchase new microtomes.   I would welcome any feedback 
positive or negative.  We are currently looking at HM355 S manufactured in 2018.

Thank you for your help,


Silvia Bonner, BS, HT(ASCP) CM

Histology Supervisor



sbon...@pathregional.com

Pathologists’ Regional Laboratory

Anatomical Pathology

2841 Juniper Dr. Suite 1

Lewiston, ID 83501

(208)746-5533



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confidential or privileged for the sole use of the intended recipient. YOU ARE 
NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF 
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are not the intended recipient, please notify us immediately by telephone at 
(208) 746-0516 or (208) 746-5533 and delete all copies.



This email may contain physician, patient and/or attorney material that is 
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are not the intended recipient, please notify us immediately by telephone at 
(208) 746-0516 or (208) 746-5533 and delete all copies.

Pathologist's Regional Laboratory 

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[Histonet] Microtome Suggestions/Demos

[Charles Riley via Histonet]

My company is seeking thoughts on new microtomes to purchase.  We have demoed a 
Sakura and Leica HistoCore BioCut but would like to test out at least 1/2 more 
options before deciding on where to proceed.

Does anyone have any suggestions on microtomes that are good for both 
experienced tech and new graduates?

We are also specifically searching for microtomes that have the capability to 
reverse the advance wheel directions (i.e spinning away from the body moves the 
block holder backwards but can be changed to do the reverse back on tech 
preference)
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Re: [Histonet] Microtome at home

[Patsy Ruegg via Histonet]

I have done it, but you are right, I had my own private business, not sure why 
it would be a problem, especially for research.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



From: Jamie Watson 
Sent: Wednesday, April 15, 2020 5:44 PM
To: Histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Microtome at home

Hello all,

Our pathologist has come up with the idea of sending a microtome and waterbath 
home to someone that cannot come to work due to COVID 19.  We are a research 
lab and work with mouse and rat tissue.  Does anyone know of any issues with 
doing this?  I have never heard of anyone cutting slides at home other than 
someone with a private business.

Thank you.

Jamie

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Re: [Histonet] Microtome at home

[raestask via Histonet]

I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from 
my Verizon, Samsung Galaxy smartphone
 Original message From: Jamie Watson via Histonet 
 Date: 4/15/20  6:44 PM  (GMT-06:00) To: 
Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello 
all,Our pathologist has come up with the idea of sending a microtome and 
waterbath home to someone that cannot come to work due to COVID 19.  We are a 
research lab and work with mouse and rat tissue.  Does anyone know of any 
issues with doing this?  I have never heard of anyone cutting slides at home 
other than someone with a private business.Thank 
you.Jamie___Histonet mailing 
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[Histonet] Microtome at home

[Jamie Watson via Histonet]

Hello all,

Our pathologist has come up with the idea of sending a microtome and waterbath 
home to someone that cannot come to work due to COVID 19.  We are a research 
lab and work with mouse and rat tissue.  Does anyone know of any issues with 
doing this?  I have never heard of anyone cutting slides at home other than 
someone with a private business.

Thank you.

Jamie
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[Histonet] Microtome point of views

[Blanca Lopez via Histonet]

Morning everybody!
I want to ask if anybody had been use the Microtome from Ted Pella HTM 2258...

Blanca Lopez HT (ASCP)cm
Senior Histotechnologist

UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center
UTSTR Biorepository Tissue Lab
5323 Harry Hines Blvd K1-210
214-648-7598
blanca.lo...@utsouthwestern.edu





UT Southwestern


Medical Center



The future of medicine, today.

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[Histonet] Microtome Accident Prevention Ideas

[Pairan, Kelly via Histonet]

Good Afternoon Histonetters,
Three weeks ago, I was cutting at a manual microtome and when I finished laying 
my ribbon on the waterbath and turned back to my block, I happened to nick my 
finger on the end of my microtome blade.  It was a freak accident of my finger 
being in the wrong place at the wrong time.  I required 2 stitches.  No big 
deal.  Histotechs often have scars.  In fact this is my second one on this 
finger.  The last incident was over 10 years ago at another institution.  I was 
invited today to a meeting of some higher level management to discuss the 
incident and to come up with interventions to prevent these incidents in the 
future.  This was a freak accident that couldn't have been prevented, in my 
opinion. It is impossible to transfer a ribbon to your waterbath and put the 
blade guard up at the same time.  I do want to come to the meeting with ideas 
though because I am walking into a room full of people who have never laid eyes 
on a microtome and I don't want them to come up with ideas for me.  Here are 
some measures that we already have in place:
1) We always lock our wheel when we are not turning it.
2) We put our blade guard up when we are changing out a block or when we walk 
away from our microtome.
3) After cleaning our microtomes, we put them back together and check to make 
sure they are locked appropriately.
4) We never leave a blade in the microtome when we are leaving for the day.

Thanks for your help,
Kelly

Kelly Pairan,  HT (ASCP)CM, QIHC (ASCP)
Histology Supervisor-Anatomic Pathology
Department of Pathology and Laboratory Medicine
Email:  kelly.pai...@nationwidechildrens.org
ph: 614-722-5414
fx: 614-722-3033

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[Histonet] microtome reviews

[Lauren Sweeney via Histonet]

Hi Everyone! We are looking into getting an embedding center and I was 
wondering if I could get some reviews from you about them. Specifically we are 
looking at the Tanner Scientific TN1600, the Avantik EM8, and the Leica 
Arcadia. Any thoughts on any of these would be much appreciated!


Have a wonderful day,
Lauren
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[Histonet] microtome woes

[Underwood, Fred via Histonet]

Greetings to all,

I have a microtome, purchased in 2013, that now needs both circuit boards 
replaced.  For the low, low price of $6400.  This seems like it may not be far 
off from the price of a new unit.  Would some of you be willing to share what 
brand of microtome you have, and what you paid?  I am hesitant to share on a 
public forum what my unit is, but privately


Thanks for your input,
Fred
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[Histonet] Microtome Knife Holder

[Maggie DeBell via Histonet]

Hello,


I am an undergraduate student looking for a universal knife holder for the
Microm HM 355 S-2 microtome I am using. Does anybody have one that they
can't use anymore, or know where I could find one inexpensively?


Thank you,


Maggie DeBell
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[Histonet] Microtome maintenance

[Johnson, Teri via Histonet]

Hi fellow histonetters,

I am interested in finding out what those of you in CAP/CLIA labs are doing for 
routine maintenance of your microtomes, especially for smaller volume labs. Do 
you have service contracts set up? Or regular (annual or every other year) 
maintenance schedules? The cost of "periodic inspection" is pretty significant.

Thanks in advance and Happy Friday!

Teri Johnson, HT(ASCP)QIHC
Manager, Histopathology and IHC

T +1 760 602 1402 (new phone number)
teri.john...@navigatebp.com

Navigate BioPharma Services, Inc.
A Novartis Subsidiary
1890 Rutherford Rd.,
Carlsbad, CA 92008
USA

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[Histonet] Microtome needed

[Blanca Lopez via Histonet]

Histoneths!!!
I have a doctor looking to buy a Histology instruments mainly microtome used or 
new. Does somebody knows any company or vendor that I can contact?
thanks


Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




UT Southwestern


Medical Center



The future of medicine, today.

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Re: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades

[Jessica Riggleman via Histonet]

Hello,

I send mine out to IMEB in California. You may be able to sell the blades on 
Ebay.

Thank you,
Jessica



_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
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affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


-Original Message-
From: Michelle Aono [mailto:aono...@auburn.edu]
Sent: Wednesday, October 26, 2016 10:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome Cleaning Company and What to do with Old Metal 
Blades

Can anyone suggest a good company that works on old microtomes?  Ours is not 
broken, it's just in need of some TLC.  Also, what is a reasonable price for 
such work (we have a RJ 2040)?

Also, we have a lot of the old metal blades that you have to sharpen.  Any 
suggestions on how to sell, donate, recycle such items?  Do people even use 
these anymore?  I would hate to have them thrown out.

Thanks!
Michelle (Shelly) Aono
~~~
Research Associate II
107B/124 Greene Hall
Auburn University, Dept of APP
Auburn, AL 36849
(334) 844-5594


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Re: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades

[Carol G Fields via Histonet]

Hi,
I have used Michael Dietrich and his team at Southeast Pathology Instrument 
Servicefor years.  They are the best and the nicest!
Carole

Carole Fields, HT (ASCP)
Lead Histotechnologist, Pathology Laboratory
Martin Luther King Jr. Community Hospital

1680 E. 120th Street
Los Angeles, CA 90059
O:424-338-8000 x 8341
cfie...@mlkch.org


-Original Message-
From: Drew Meyer via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, October 26, 2016 9:59 AM
To: Michelle Aono <aono...@auburn.edu>
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Cleaning Company and What to do with Old 
Metal Blades

Michael Dietrich and his team at Southeast Pathology Instrument Service is 
outstanding!

www.southeastpathology.com<http://www.southeastpathology.com>

Thanks,
Drew Meyer, HT
Anatomic Pathology Independent Contractor

On Wed, Oct 26, 2016 at 10:56 AM, Michelle Aono via Histonet < 
histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> 
wrote:

> Can anyone suggest a good company that works on old microtomes?  Ours
> is not broken, it's just in need of some TLC.  Also, what is a
> reasonable price for such work (we have a RJ 2040)?
>
> Also, we have a lot of the old metal blades that you have to sharpen.
> Any suggestions on how to sell, donate, recycle such items?  Do people
> even use these anymore?  I would hate to have them thrown out.
>
> Thanks!
> Michelle (Shelly) Aono
> ~~~
> Research Associate II
> 107B/124 Greene Hall
> Auburn University, Dept of APP
> Auburn, AL 36849
> (334) 844-5594
>
> ___
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Re: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades

[Drew Meyer via Histonet]

Michael Dietrich and his team at Southeast Pathology Instrument Service is
outstanding!

www.southeastpathology.com

Thanks,
Drew Meyer, HT
Anatomic Pathology Independent Contractor

On Wed, Oct 26, 2016 at 10:56 AM, Michelle Aono via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Can anyone suggest a good company that works on old microtomes?  Ours is
> not broken, it's just in need of some TLC.  Also, what is a reasonable
> price for such work (we have a RJ 2040)?
>
> Also, we have a lot of the old metal blades that you have to sharpen.  Any
> suggestions on how to sell, donate, recycle such items?  Do people even use
> these anymore?  I would hate to have them thrown out.
>
> Thanks!
> Michelle (Shelly) Aono
> ~~~
> Research Associate II
> 107B/124 Greene Hall
> Auburn University, Dept of APP
> Auburn, AL 36849
> (334) 844-5594
>
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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[Histonet] Microtome Cleaning Company and What to do with Old Metal Blades

[Michelle Aono via Histonet]

Can anyone suggest a good company that works on old microtomes?  Ours is not 
broken, it's just in need of some TLC.  Also, what is a reasonable price for 
such work (we have a RJ 2040)?

Also, we have a lot of the old metal blades that you have to sharpen.  Any 
suggestions on how to sell, donate, recycle such items?  Do people even use 
these anymore?  I would hate to have them thrown out.

Thanks!
Michelle (Shelly) Aono
~~~
Research Associate II
107B/124 Greene Hall
Auburn University, Dept of APP
Auburn, AL 36849
(334) 844-5594

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Re: [Histonet] microtome validation

[Jacox, Robert A. via Histonet]

Ann,

I would recommend validating manual microtomes more often then automatic. Any 
traditional gear driven microtome does change overtime as gears wear. This 
eventually will lead to the micron indicator on the microtome not reading what 
you are truly cutting. Automated, microtomes driven by stepper motors tend to 
remain accurate longer but you may want to document the accuracy every couple 
of years. 

To check calibration most service engineers will mount a micrometer to the 
microtome and measure the feed of the microtome as the hand wheel turns. The 
procedure generally takes 5 minutes. Check with your microtome manufacture to 
see if they can assist you.

Robert Jacox
Thermo Fisher Scientific
Commercial Marketing Manager 



Sent from my iPad

> On Jul 9, 2016, at 1:39 PM, Ann Specian via Histonet 
>  wrote:
> 
> Has anyone had to validate a microtome (manual/automatic).  If so, how did 
> you do it?
> Ann
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[Histonet] microtome validation

[Ann Specian via Histonet]

Has anyone had to validate a microtome (manual/automatic).  If so, how did you 
do it?
Ann
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[Histonet] Microtome response and unrelated blog psot

[Lester Raff MD via Histonet]

We are very satisfied with Leica microtomes and will probably be getting an 
additional one soon. Yes, they are adjustable.

Unrelated blog post:
http://www.chicagonow.com/downsize-maybe/2016/04/whats-in-your-wallet-theres-a-little-aluminum-foil-package-in-mine/
Please note, if you cut and paste, grab that last "/"

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] Microtome workstations

[Richardson, Pam K via Histonet]

Hi, we are looking for individual microtome workstations.  What are you using 
for your microtome workstations? Are they adjustable? 

Cordially,

Pam ~
+++
Pam Richardson
Clinical Manager
Gundersen Health System Laboratory Services
Email: pkric...@gundersenhealth.org
Phone: 608 775-4133
Fax: 608 775-6136
Interdepartmental Mail Stop: H04-007
E-visit us at: http://www.gundersenhealth.org



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[Histonet] MICROTOME KNIFE SHARPENING

[Hannen, Valerie via Histonet]

Hi all...  Hoping you might be answer a couple of questions that I have.  1) 
Does anyone use a microtome knife sharpening kit made by Pathco?

If so, 2) How well does it sharpen the blades?  3) Is there any issue on 
attaching the abrasive sheets to the honing plate?  4) Do the abrasive sheets 
tend to "move"

during the sharpening process, that would cause the blade not be on them but on 
the plate instead?

  I currently am using coarse and fine abrasive liquids along with the honing 
plate... but the coarse, fine and honing liquids are becoming quite expensive 
and hard to come by.

Any and all replies are welcomed.

Thank you in advance,



Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com

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Re: [Histonet] microtome issues: thick and no sections (Tyrone Genade)

[Tyrone Genade via Histonet]

Hello,

Thanks to all who responded to my question. The solution, it seems was very
simple.

Several people reported similar experiences and issues with the specimen
clamp not fitting tightly and that I should clean the clamp or block. My
blocks are clean and I thought the clamp was too... but when I ran a set of
forceps along the inner, upper edge of the clamp a tiny blob of wax was
found. A test block cut normally.

I'm amazed a tiny piece of wax could cause so much  trouble!

Thanks to everyone who offered help.

Bye
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[Histonet] microtome issues: thick and no sections

[Tyrone Genade via Histonet]

Hello,

I am having a problem with sectioning.

I'm cutting 5 um sections but with the turn of the microtome I will miss
the block, or take a small piece of the block/tissue and then the next
section ends up thick. This missing can take several turns of the wheel so
I can get really big sections. I have checked the angle of the knife to the
block and the tension along the blade but these are fine. I worry that the
issue might be temperature. The microtome is on a bench against an outside
wall. The temperature is about 15 oC. Could this be the issue?

I am not cooling the block on ice. Previous experience with block-cooling
was that I would get thick and thin sections that way... this is a very
different problem.

Thanks

-- 
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org

Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.
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[Histonet] Microtome suggestions

[Goetz, Freya E. via Histonet]

Good morning!
I was wondering if anyone has microtome opinions they would like to share. We 
have an ultramicrotome but I am looking for a microtome that could cut larger 
pieces of tissue embedded in plastic. I am hesitant to go with Leica because 
service is so expensive but I’m looking for honest opinions.

Thanks and have a nice day!

Freya
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Re: [Histonet] Microtome suggestions

[Rene J Buesa via Histonet]

For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) 
manufactures the best, but you could try an OMS from Reichert although I am not 
sure they stll are manufactured because Leica swallowed Reichert some years 
ago.Another alternative, and probably even better than the Leitz (Leica), is a 
Spencer (American Optical) sliding microtome Model 860 For me this is the best 
all-around ever manufactured sliding microtome and not too long ago a saw one 
for sale in e-Bay. Why don't you try looking for it. It will be much cheaper 
than any modern horizontal microtome and far better than any new one.René J. 


 On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" 
 wrote:
   

 Good morning!
I was wondering if anyone has microtome opinions they would like to share. We 
have an ultramicrotome but I am looking for a microtome that could cut larger 
pieces of tissue embedded in plastic. I am hesitant to go with Leica because 
service is so expensive but I’m looking for honest opinions.

Thanks and have a nice day!

Freya
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Re: [Histonet] Microtome suggestions

[Goetz, Freya E. via Histonet]

Hi Rene and histonet,

Thank you for your suggestions. I must amend my earlier request. We have an 
ultra microtome from RMC and a Spencer 820 microtome. I would like to section 
epoxy embedded material that is larger than an ultramicrotome could handle but 
smaller than a sledge microtome. Sorry I wasn’t specific.

Thank you again!

Freya


On Oct 7, 2015, at 12:01 PM, Rene J Buesa 
> wrote:

For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) 
manufactures the best, but you could try an OMS from Reichert although I am not 
sure they stll are manufactured because Leica swallowed Reichert some years ago.
Another alternative, and probably even better than the Leitz (Leica), is a 
Spencer (American Optical) sliding microtome Model 860
For me this is the best all-around ever manufactured sliding microtome and not 
too long ago a saw one for sale in e-Bay.
Why don't you try looking for it. It will be much cheaper than any modern 
horizontal microtome and far better than any new one.
René J.



On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" 
> 
wrote:


Good morning!
I was wondering if anyone has microtome opinions they would like to share. We 
have an ultramicrotome but I am looking for a microtome that could cut larger 
pieces of tissue embedded in plastic. I am hesitant to go with Leica because 
service is so expensive but I’m looking for honest opinions.

Thanks and have a nice day!

Freya
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Re: [Histonet] Microtome suggestions

[Hawkins, Hal K. via Histonet]

The old Sorvall JB-4 microtome was designed for this purpose and works well.  
They are available at a reasonable price from several used equipment providers 
and on Ebay. 



From: Goetz, Freya E. via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Wednesday, October 07, 2015 11:07 AM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome suggestions

Hi Rene and histonet,

Thank you for your suggestions. I must amend my earlier request. We have an 
ultra microtome from RMC and a Spencer 820 microtome. I would like to section 
epoxy embedded material that is larger than an ultramicrotome could handle but 
smaller than a sledge microtome. Sorry I wasn’t specific.

Thank you again!

Freya


On Oct 7, 2015, at 12:01 PM, Rene J Buesa 
<rjbu...@yahoo.com<mailto:rjbu...@yahoo.com>> wrote:

For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) 
manufactures the best, but you could try an OMS from Reichert although I am not 
sure they stll are manufactured because Leica swallowed Reichert some years ago.
Another alternative, and probably even better than the Leitz (Leica), is a 
Spencer (American Optical) sliding microtome Model 860
For me this is the best all-around ever manufactured sliding microtome and not 
too long ago a saw one for sale in e-Bay.
Why don't you try looking for it. It will be much cheaper than any modern 
horizontal microtome and far better than any new one.
René J.



On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" 
<histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> 
wrote:


Good morning!
I was wondering if anyone has microtome opinions they would like to share. We 
have an ultramicrotome but I am looking for a microtome that could cut larger 
pieces of tissue embedded in plastic. I am hesitant to go with Leica because 
service is so expensive but I’m looking for honest opinions.

Thanks and have a nice day!

Freya
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[Histonet] microtome blade

[Zerfas, Patricia (NIH/OD/ORS) [E] via Histonet]

Dear Histologists,
After you have completed sectioning for the day do you remove the blade?  Is it 
safer to keep the blade locked in place?  Is it a safety issue?  Or is it a 
policy set by your workplace?

Thanks,

Patricia M. Zerfas
National Institutes of Health
Bldg 28A Room 112 MSC 5230
9000 Rockville Pike
Bethesda, MD  20895  USA
(301) 496-4464
(301) 402-1068

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Re: [Histonet] microtome blade

[Jay Lundgren via Histonet]

NEVER leave a blade in a microtome when not sectioning!

  Sincerely,

Jay A. Lundgren, M.S., HTL
(ASCP)

On Wed, Aug 19, 2015 at 9:12 AM, Zerfas, Patricia (NIH/OD/ORS) [E] via
Histonet histonet@lists.utsouthwestern.edu wrote:

 Dear Histologists,
 After you have completed sectioning for the day do you remove the blade?
 Is it safer to keep the blade locked in place?  Is it a safety issue?  Or
 is it a policy set by your workplace?

 Thanks,

 Patricia M. Zerfas
 National Institutes of Health
 Bldg 28A Room 112 MSC 5230
 9000 Rockville Pike
 Bethesda, MD  20895  USA
 (301) 496-4464
 (301) 402-1068

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[Histonet] Microtome ergonimic evaluation

[Kristina Wyatt]

Has anyone ever had an ergonomic evaluation specific to their microtome 
station?  If so, could you please give me the contact information of the 
evaluator?

Thanks,

Kristina Wyatt, M.A.
Supervisor: Histopathology/Immunohistochemistry
L216 Mosier Hall
Kansas State Veterinary Diagnostic Laboratory
785-532-4464
kdwy...@vet.k-state.edu

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Re: [Histonet] microtome stage

[Alan Bright]

Donna, I would take the powder coating off with a paint stripper, try 
sectioning, if happy have the holder electro plated which will be a conductive 
coating that will protect the base metal from corrosion. Cost under $20.
BR Alan Bright, BIC...Cambs..UK

Sent from my iPhone

 On 30 Sep 2014, at 02:53, Donna Maney dmane...@yahoo.com wrote:
 
 I have a Leica SM2000 microtome, circa 2004, that has recently developed an 
 issue with the dry ice tray. Up until this year, we've never had any problems 
 with the specimen adhering to the platform. But suddenly we cannot cut 
 specimens because they easily become dislodged. We use 30% sucrose to stick 
 the specimen to the dry ice tray. When we borrow a dry ice tray from another 
 lab, the problem goes away.
 
 Our dry ice tray is powder coated and we can see the the coating is rubbing 
 away, but it does not seem to be coming off with the specimen. Rotating the 
 tray so that the specimen is attached at a different location on the platform 
 does not help. We have cleaned the dry ice tray with hot soapy water, rinsed 
 with distilled water and then with ethanol. 
 
 Leica told me that the new trays do not have this powder coating, which makes 
 me think they know about a problem, but the person I spoke to on the phone 
 was not aware of one.
 
 I'd be glad to hear from anyone who has experienced the same thing or who has 
 suggestions. A new tray will cost $500-$1000 so I'd like to troubleshoot with 
 this one to the extent that it is appropriate.
 
 Thanks,
 Donna
 
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[Histonet] microtome laceration not an exposure

[Bitting, Angela K.]

Does anyone have something that I can show to nursing to explain why a 
microtome laceration is not an exposure? I can't get the fixed tissue 
explanation to sink in. Anyone else had issues with this?




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[Histonet] Microtome for HS Biotech Class

[Patsy Ruegg]

Dear Histo Peeps,

I am on the advisory board for a local high school Biotech class and have been 
helping them process, embed, cut, and stain tissue (they use chicken livers 
can't use human).  My husband fixed up an old black AO microtome for them a few 
years ago so they actually cut sections.  This year the teacher contacted me 
and told me that her usual class of about 20-24 has gone to 32 and she doesn't 
think she will have enough time to teach everyone to cut a section with just 
one microtome.  She asked me if I knew anyone locally they could borrow a 
microtome from, so I have been asking around but since I am retired I do not 
have access to many resources anymore.  I thought I would put this out to our 
histo community especially if there is someone local in Colorado that could 
help us out.  I also thought that one of the equipment vendors like Rankin 
might be able to help out.  This is a Cherry Creek High School (Overland) and 
the teacher said the school would give out receipts for tax purposes for any 
donations received.  Thank you all for considering this request.

Best regards,
Patsy

PS
Maybe our past and new Region VII directors (Janet and Jane) could help with 
this???

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net
  
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Re: [Histonet] Microtome calibration

[Pam Marcum]

We have a service contract with an annual PM.  The aligments and calibration 
are done then. 



Pam 




- Original Message -
From: Michael Ann Jones mjo...@metropath.com 
To: Ronda Mire rm...@cvpath.org, Denise Long denise.l...@uconn.edu 
Cc: histonet@lists.utsouthwestern.edu 
Sent: Wednesday, July 16, 2014 8:07:37 AM 
Subject: Re: [Histonet] Microtome calibration 

We do ours by hand? You mean line them up or PM? 
Michael Ann Jones, HT (ASCP) 
Histology Manager 
Metropath 
7444 W. Alaska Dr. #250 
Lakewood, CO 80226 
303.634.2511 
mjo...@metropath.com 




On 7/15/14, 1:55 PM, Ronda Mire rm...@cvpath.org wrote: 

Hi Denise, 
 
Check with the company that does your PM service.  This may be something 
that is already done during preventative maintenance. 
 
On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote: 
 
 Hi Everyone, 
 During a recent inspection we were asked by the inspection team to 
start calibrating our microtomes.  Anyone know of a service vendor that 
will do this? Referrals, including self-referrals would be greatly 
appreciated. 
 Thank, 
 
 Denise M. Long, MS, HTL (ASCP), QIHC 
 University of Connecticut 
 Dept. of Pathobiology and Veterinary Sciences 
 Connecticut Veterinary Medical Diagnostic Laboratory 
 61 N. Eagleville Road, Unit 3089 
 Storrs, Connecticut 06269-3089 
 (860) 486-0851 
 
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[Histonet] Microtome calibration

[Long, Denise]

Hi Everyone,
During a recent inspection we were asked by the inspection team to start 
calibrating our microtomes.  Anyone know of a service vendor that will do this? 
Referrals, including self-referrals would be greatly appreciated.
Thank,

Denise M. Long, MS, HTL (ASCP), QIHC
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences
Connecticut Veterinary Medical Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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Re: [Histonet] Microtome calibration

[Ronda Mire]

Hi Denise,

Check with the company that does your PM service.  This may be something that 
is already done during preventative maintenance.

On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote:

 Hi Everyone,
 During a recent inspection we were asked by the inspection team to start 
 calibrating our microtomes.  Anyone know of a service vendor that will do 
 this? Referrals, including self-referrals would be greatly appreciated.
 Thank,
 
 Denise M. Long, MS, HTL (ASCP), QIHC
 University of Connecticut
 Dept. of Pathobiology and Veterinary Sciences
 Connecticut Veterinary Medical Diagnostic Laboratory
 61 N. Eagleville Road, Unit 3089
 Storrs, Connecticut 06269-3089
 (860) 486-0851
 
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Re: [Histonet] Microtome calibration

[Michael Ann Jones]

We do ours by hand? You mean line them up or PM?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 7/15/14, 1:55 PM, Ronda Mire rm...@cvpath.org wrote:

Hi Denise,

Check with the company that does your PM service.  This may be something
that is already done during preventative maintenance.

On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote:

 Hi Everyone,
 During a recent inspection we were asked by the inspection team to
start calibrating our microtomes.  Anyone know of a service vendor that
will do this? Referrals, including self-referrals would be greatly
appreciated.
 Thank,
 
 Denise M. Long, MS, HTL (ASCP), QIHC
 University of Connecticut
 Dept. of Pathobiology and Veterinary Sciences
 Connecticut Veterinary Medical Diagnostic Laboratory
 61 N. Eagleville Road, Unit 3089
 Storrs, Connecticut 06269-3089
 (860) 486-0851
 
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RE: [Histonet] Microtome calibration

[Weems, Joyce K.]

Could they have meant an aligner -  something like this?

http://www.newcomersupply.com/products/microtome-cryostat-aligners?page=M#1130


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Long, Denise
Sent: Tuesday, July 15, 2014 3:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome calibration

Hi Everyone,
During a recent inspection we were asked by the inspection team to start 
calibrating our microtomes.  Anyone know of a service vendor that will do this? 
Referrals, including self-referrals would be greatly appreciated.
Thank,

Denise M. Long, MS, HTL (ASCP), QIHC
University of Connecticut
Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical 
Diagnostic Laboratory
61 N. Eagleville Road, Unit 3089
Storrs, Connecticut 06269-3089
(860) 486-0851

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Re: [Histonet] Microtome

[Ronda Mire]

Agree, Leica is the best hands down.

On Jun 3, 2014, at 5:37 PM, nmhi...@comcast.net wrote:

 If someone promised me a brand new FREE microtome and it wasn't a LEICA, I'd 
 tell them, no, thanks!.  I am not inclined to endorse any other brand and I 
 cannot imagine changing my mind about that. 
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Re: [Histonet] Microtome

[Tony Auge]

Of the three microtomes that you listed I would highly recommend the Thermo
Shandon Finesse. It's of superior quality than the Microms I have used.
Cutting is excellent and the fly wheel action is light yet fluid. You do
have to oil it manually once a month but it's easy and you can clean it out
in the process and keep it in good operating condition. I haven't used the
Titan 5000 but I have used slides from Tanner that were of poor quality and
I ended up returning them.

I'm cutting on a used Leica 2125 right now. It is functional but it does
skip when facing and block orientation is terrible. Getting used lab
equipment is always a gamble so make sure your supplier is pleasant to deal
with and offers a warranty. Not sure what your budget is but a new Finesse
isn't too expensive for a new microtome and I'm sure you will be very happy
with it.


Tony Auge HTL (ASCP) QIHC
Histology Supervisor - Chandler Pathology Services
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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RE: [Histonet] Microtome

[Susan.Walzer]

Leica 2125

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 02, 2014 10:44 PM
To: Sylvia Shockey
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome

Leica all the way 2025

Sent from my iPhone

 On Jun 2, 2014, at 5:19 PM, Sylvia Shockey 
 sylvia.shoc...@clinicalpathologyassoc.com wrote:
 
 
 Hello,
   My name is Sylvia Shockey I work in a histology lab. One of 
 our microtomes is not working properly and would appreciate some advice on 
 purchasing a new one or maybe refurbished.  I have had some quotes on a 
 Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon 
 Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has 
 new and used on both of these models. Help me please I don't want to buy a 
 clunker.  Thanks for your time.
 
 
 
 
 
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RE: [Histonet] Microtome

[steven Mello]

Leica is an outstanding product.  Highly recommend there microtome.

Steven Mello,HT(ASCP)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 2, 2014 10:44 PM
To: Sylvia Shockey
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome

Leica all the way 2025

Sent from my iPhone

 On Jun 2, 2014, at 5:19 PM, Sylvia Shockey
sylvia.shoc...@clinicalpathologyassoc.com wrote:
 
 
 Hello,
   My name is Sylvia Shockey I work in a histology lab. One
of our microtomes is not working properly and would appreciate some advice
on purchasing a new one or maybe refurbished.  I have had some quotes on a
Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon
Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has
new and used on both of these models. Help me please I don't want to buy a
clunker.  Thanks for your time.
 
 
 
 
 
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RE: [Histonet] Microtome

[Anne Murvosh]

Most companies will let you test run their newest microtomes. Let them
come to you and test for a week or so than pick the one you like best.
Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sylvia
Shockey
Sent: Monday, June 02, 2014 2:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome


Hello,
   My name is Sylvia Shockey I work in a histology lab.
One of our microtomes is not working properly and would appreciate some
advice on purchasing a new one or maybe refurbished.  I have had some
quotes on a Tanner scientific manual rotary microtome, titan 5000 .
Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with
Thermo fisher has new and used on both of these models. Help me please I
don't want to buy a clunker.  Thanks for your time.





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Re: [Histonet] Microtome

[Rene J Buesa]

When a microtome starts to act up the best solution is to give it an 
overhaul. If with the possibility of buying one, contact Leica or Sakura first. 
Both manufacture very reliable and durable instruments.
René J.  


On Tuesday, June 3, 2014 10:12 AM, Anne Murvosh amurv...@advancederm.net 
wrote:
  


Most companies will let you test run their newest microtomes. Let them
come to you and test for a week or so than pick the one you like best.
Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sylvia
Shockey
Sent: Monday, June 02, 2014 2:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome


Hello,
                   My name is Sylvia Shockey I work in a histology lab.
One of our microtomes is not working properly and would appreciate some
advice on purchasing a new one or maybe refurbished.  I have had some
quotes on a Tanner scientific manual rotary microtome, titan 5000 .
Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with
Thermo fisher has new and used on both of these models. Help me please I
don't want to buy a clunker.  Thanks for your time.





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RE: [Histonet] Microtome

[Bea DeBrosse-Serra]

Leica 2125

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Monday, June 02, 2014 7:44 PM
To: Sylvia Shockey
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome

Leica all the way 2025

Sent from my iPhone

 On Jun 2, 2014, at 5:19 PM, Sylvia Shockey 
 sylvia.shoc...@clinicalpathologyassoc.com wrote:
 
 
 Hello,
   My name is Sylvia Shockey I work in a histology lab. One of 
 our microtomes is not working properly and would appreciate some advice on 
 purchasing a new one or maybe refurbished.  I have had some quotes on a 
 Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon 
 Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has 
 new and used on both of these models. Help me please I don't want to buy a 
 clunker.  Thanks for your time.
 
 
 
 
 
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Re: [Histonet] Microtome

[nmhisto]

If someone promised me a brand new FREE microtome and it wasn't a LEICA, I'd 
tell them, no, thanks!.  I am not inclined to endorse any other brand and I 
cannot imagine changing my mind about that. 
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[Histonet] Microtome

[Sylvia Shockey]

Hello,
   My name is Sylvia Shockey I work in a histology lab. One of 
our microtomes is not working properly and would appreciate some advice on 
purchasing a new one or maybe refurbished.  I have had some quotes on a Tanner 
scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a 
Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on 
both of these models. Help me please I don't want to buy a clunker.  Thanks for 
your time.





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Re: [Histonet] Microtome

[Sue]

Leica all the way 2025

Sent from my iPhone

 On Jun 2, 2014, at 5:19 PM, Sylvia Shockey 
 sylvia.shoc...@clinicalpathologyassoc.com wrote:
 
 
 Hello,
   My name is Sylvia Shockey I work in a histology lab. One of 
 our microtomes is not working properly and would appreciate some advice on 
 purchasing a new one or maybe refurbished.  I have had some quotes on a 
 Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon 
 Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has 
 new and used on both of these models. Help me please I don't want to buy a 
 clunker.  Thanks for your time.
 
 
 
 
 
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RE: [Histonet] Microtome Service

[joelle weaver]

Thanks for this information Joyce. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
From: joyce.we...@emoryhealthcare.org
To: Histonet@lists.utsouthwestern.edu
Date: Tue, 17 Dec 2013 23:03:02 +
CC: 
Subject: [Histonet] Microtome Service

Hello Folks,
 
I know that you know that every time someone mentions Leitz or Leica problems 
with a microtome or a cryotstat, I send out the name of Klaus Dern, my good 
friend for so many years I've lost count.
 
I want to let you know about something he can do that save your labs a lot of 
money, if you want service instead of replacing expensive parts because the one 
present on the newer microtomes can not be repaired.
 
For the following microtomes,
Leica RM2125
Leica 2030 Biocut
Leica/Jung 2035
Leica CM-1850 Cryostat
Sakura SRM200
 
The advance mechanism can not be repaired, according to the company. Klaus can 
retool or whatever you call it,  the advance mechanism. Excessive play can 
develop in the advance mechanism, causing chunking of the block and the agony 
and gnashing of teeth. The company will tell you the whole part needs to be 
replaced or perhaps you even need to get a new microtome.
 
Micro Precision Instruments Co (none other than Klaus Dern) can repair the 
instrument for about half of what the new part would cost.  The best thing 
about the repair is that it becomes adjustable and can forever work with little 
maintenance.
 
I just wanted to share this with you - I am not the vendor. I just want to let 
you know of something that can help in these trying times of budget constraints 
and replacing equipment. He can repair the microtomes out of the cryostats. You 
can remove and send to him. He has loaners (I asked).   I just had a cryostat 
repaired. He is close enough to me that he can come on site and I don't need to 
ship.
 
Here is his info for those who might be interested.
Phone: 706-635-8840
Fax: 706-635-3074
klaus.der...@gmail.com
 
Hope you find this helpful!
 
Hope everyone is enjoying the hustle and bustle of the season. Merry Christmas, 
ya'll!!   j
 
 
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
 
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
 
This e-mail, including any attachments is the property of Saint Joseph’s 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.
 

 
This e-mail message (including any attachments) is for the sole use of
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[Histonet] microtome

[Rui TAHARA]

Hello, 
 
I have undecalcified biological sample embedded in plastic media (MMA). 
I am looking for faclities that offers self-service microtome (for plastic 
embedding sectioining) or short time rental microtome with some training around 
Quebec, Ontraio, NY. I have been trying to find ones in Montreal, however, its 
been difficult to find self-service microtome. 
I appreciate if you would provide some information for this. 
 
Thank you in advance, 
 
Rui 
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[Histonet] Microtome Blade safety, in or out when not in use?

[Nancy Schmitt]

Hi Leah-
We leave the blade in behind the guard and we also lock the wheel when we leave 
the microtome.
Nancy
Histology Coordinator
Dubuque, IA
___
Message: 8
Date: Fri, 25 Oct 2013 12:33:43 +1100
From: Leah Simmons leah_simmon...@hotmail.com
Subject: [Histonet] Microtome Blade safety, in or out when not in use?
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID: col129-w78785db134d1d1265ca33ff8...@phx.gbl
Content-Type: text/plain; charset=iso-8859-1

Hello all :-)
I am doing a quick microtome blade safety survey,
When you finish work, do you leave your blade in the microtome behind the blade 
guard or do you take it out?
If you take it out and it is a new blade or a blade still useful for trimming  
where do you store it?
Thank you for your feedback, I really appreciate it.
Regards
Leah Simmons



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Re: [Histonet] Microtome Blade safety, in or out when not in use?

[Paula Sicurello]

Left in but covered with the blade guard.  Not spanking new, but usable
(for facing) get stored in an old box that the slides came in.  I like the
slide mailer idea, and will switch to that.

-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091

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hereby notified that you have received this document in error and that any
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contents of this information is strictly prohibited . If you have received
this communication in error, please notify us immediately by e-mail, and
delete the original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey,
 When you finish work, do you leave your blade in the microtome behind the
 blade guard or do you take it out?
 If you take it out and it is a new blade or a blade still useful for
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[McAnn, Sherrian]

I was taught that when leaving your microtome for any length of time to
always take the blade out. We had a tech that had the habit of leaving
the blade on her microtome and even though she had the safety guard up
someone from  biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the
next time then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a
blade is used and taken out then it should be thrown away..because the
more it is being handled the greater the chance of injury. Just saying
what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in
use?

Left in but covered with the blade guard.  Not spanking new, but usable
(for facing) get stored in an old box that the slides came in.  I like
the slide mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University
Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents
are covered by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, and contain information intended for the specific individual
(s) only. This information is confidential. If you are not the intended
recipient or an agent responsible for delivering it to the intended
recipient, you are hereby notified that you have received this document
in error and that any review, dissemination, copying or the taking of
any action based on the contents of this information is strictly
prohibited . If you have received this communication in error, please
notify us immediately by e-mail, and delete the original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey, When you finish 
 work, do you leave your blade in the microtome behind the blade guard 
 or do you take it out?
 If you take it out and it is a new blade or a blade still useful for 
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[Marcum, Pamela A]

The rule here is a blade is cheaper than a cut.  Anytime you are walking away 
and returning within a few minutes to cut use the knife guard otherwise throw 
the blade out.  It is an accident looking to happen.  Recently we had a tech 
decide not use the knife guard and seriously cut himself by misjudging the 
distance from his elbow reaching an knife he was no longer able to use.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian
Sent: Friday, October 25, 2013 8:46 AM
To: Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use?

I was taught that when leaving your microtome for any length of time to always 
take the blade out. We had a tech that had the habit of leaving the blade on 
her microtome and even though she had the safety guard up someone from  
biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the next time 
then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a blade is 
used and taken out then it should be thrown away..because the more it is being 
handled the greater the chance of injury. Just saying what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in use?

Left in but covered with the blade guard.  Not spanking new, but usable (for 
facing) get stored in an old box that the slides came in.  I like the slide 
mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University Health 
System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents are 
covered by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, and contain information intended for the specific individual
(s) only. This information is confidential. If you are not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you are hereby notified that you have received this document in error and that 
any review, dissemination, copying or the taking of any action based on the 
contents of this information is strictly prohibited . If you have received this 
communication in error, please notify us immediately by e-mail, and delete the 
original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey, When you finish 
 work, do you leave your blade in the microtome behind the blade guard 
 or do you take it out?
 If you take it out and it is a new blade or a blade still useful for 
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[McAnn, Sherrian]

AMEN TO THAT!

-Original Message-
From: Marcum, Pamela A [mailto:pamar...@uams.edu] 
Sent: Friday, October 25, 2013 10:05 AM
To: McAnn, Sherrian; Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in
use?

The rule here is a blade is cheaper than a cut.  Anytime you are
walking away and returning within a few minutes to cut use the knife
guard otherwise throw the blade out.  It is an accident looking to
happen.  Recently we had a tech decide not use the knife guard and
seriously cut himself by misjudging the distance from his elbow reaching
an knife he was no longer able to use.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn,
Sherrian
Sent: Friday, October 25, 2013 8:46 AM
To: Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in
use?

I was taught that when leaving your microtome for any length of time to
always take the blade out. We had a tech that had the habit of leaving
the blade on her microtome and even though she had the safety guard up
someone from  biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the
next time then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a
blade is used and taken out then it should be thrown away..because the
more it is being handled the greater the chance of injury. Just saying
what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in
use?

Left in but covered with the blade guard.  Not spanking new, but usable
(for facing) get stored in an old box that the slides came in.  I like
the slide mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University
Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents
are covered by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, and contain information intended for the specific individual
(s) only. This information is confidential. If you are not the intended
recipient or an agent responsible for delivering it to the intended
recipient, you are hereby notified that you have received this document
in error and that any review, dissemination, copying or the taking of
any action based on the contents of this information is strictly
prohibited . If you have received this communication in error, please
notify us immediately by e-mail, and delete the original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey, When you finish 
 work, do you leave your blade in the microtome behind the blade guard 
 or do you take it out?
 If you take it out and it is a new blade or a blade still useful for 
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

 ___
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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] Microtome Blade safety, in or out when not in use?

[Morken, Timothy]

I always take it out. You never know if someone will come along and do 
something...

In our lab the mircrotomes do not strictly belong to any particular tech, and 
they are used by different people at different times of day or different 
shifts. 



Tim Morken
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leah Simmons
Sent: Thursday, October 24, 2013 6:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome Blade safety, in or out when not in use?

Hello all :-)
I am doing a quick microtome blade safety survey, When you finish work, do you 
leave your blade in the microtome behind the blade guard or do you take it out?
If you take it out and it is a new blade or a blade still useful for trimming  
where do you store it?
Thank you for your feedback, I really appreciate it.
Regards
Leah Simmons
  
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[Histonet] Microtome Blade safety, in or out when not in use? NO PHI

[Podawiltz, Thomas]

We do not re-use the blades or leave them in the microtome it is against our 
safety practices. We handle the blades twice, when we place them in the blade 
holder and when we remove them and drop them into the sharps container. 


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian
Sent: Friday, October 25, 2013 12:35 PM
To: Marcum, Pamela A; Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use?

AMEN TO THAT!

-Original Message-
From: Marcum, Pamela A [mailto:pamar...@uams.edu]
Sent: Friday, October 25, 2013 10:05 AM
To: McAnn, Sherrian; Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use?

The rule here is a blade is cheaper than a cut.  Anytime you are walking away 
and returning within a few minutes to cut use the knife guard otherwise throw 
the blade out.  It is an accident looking to happen.  Recently we had a tech 
decide not use the knife guard and seriously cut himself by misjudging the 
distance from his elbow reaching an knife he was no longer able to use.  

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian
Sent: Friday, October 25, 2013 8:46 AM
To: Paula Sicurello; Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microtome Blade safety, in or out when not in use?

I was taught that when leaving your microtome for any length of time to always 
take the blade out. We had a tech that had the habit of leaving the blade on 
her microtome and even though she had the safety guard up someone from  
biomed still  managed to lean on it and get cut (go
figure)
   If I want to save a slightly used blade to maybe trim with the next time 
then I will put it into a slide mailer (plastic with attached
lid) but that is a safety issue .  The safety officer says that when a blade is 
used and taken out then it should be thrown away..because the more it is being 
handled the greater the chance of injury. Just saying what they told me.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Friday, October 25, 2013 6:03 AM
To: Leah Simmons
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome Blade safety, in or out when not in use?

Left in but covered with the blade guard.  Not spanking new, but usable (for 
facing) get stored in an old box that the slides came in.  I like the slide 
mailer idea, and will switch to that.

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University Health 
System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents are 
covered by the Electronic Communications Privacy Act, 18 U.S.C.
2510-2521, and contain information intended for the specific individual
(s) only. This information is confidential. If you are not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you are hereby notified that you have received this document in error and that 
any review, dissemination, copying or the taking of any action based on the 
contents of this information is strictly prohibited . If you have received this 
communication in error, please notify us immediately by e-mail, and delete the 
original message.

On Thu, Oct 24, 2013 at 9:33 PM, Leah Simmons
leah_simmon...@hotmail.comwrote:

 Hello all :-)
 I am doing a quick microtome blade safety survey, When you finish 
 work, do you leave your blade in the microtome behind the blade guard 
 or do you take it out?
 If you take it out and it is a new blade or a blade still useful for 
 trimming  where do you store it?
 Thank you for your feedback, I really appreciate it.
 Regards
 Leah Simmons

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
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[Histonet] Microtome Blade safety, in or out when not in use?

[Leah Simmons]

Hello all :-)
I am doing a quick microtome blade safety survey,
When you finish work, do you leave your blade in the microtome behind the blade 
guard or do you take it out?
If you take it out and it is a new blade or a blade still useful for trimming  
where do you store it?
Thank you for your feedback, I really appreciate it.
Regards
Leah Simmons
  
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Re: [Histonet] Microtome Blade safety, in or out when not in use?

[Jennifer MacDonald]

We save the blade to use for trimming.  We store the blade in a plastic 
5-slide mailer.



From:   Leah Simmons leah_simmon...@hotmail.com
To: histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date:   10/24/2013 06:36 PM
Subject:[Histonet] Microtome Blade safety, in or out when not in 
use?
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hello all :-)
I am doing a quick microtome blade safety survey,
When you finish work, do you leave your blade in the microtome behind the 
blade guard or do you take it out?
If you take it out and it is a new blade or a blade still useful for 
trimming  where do you store it?
Thank you for your feedback, I really appreciate it.
Regards
Leah Simmons
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[Histonet] Microtome steel blade holder

[Hannen, Valerie]

Good Morning all...

I am having a problem with my Microtome steel blade holder.  One of the screws 
on the blade block that helps hold the blade tightly clamped has stripped 
itself out.  I am wondering if anyone in out there in Histo-land who no longer 
is using steel blades, but still has the knife block that is used with steel 
blades, might be willing to part with it.

I have a Leica 2025.

Thanks ever so much!!

Any and all help is greatly appreciated.

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com


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of the individual to
whom it is addressed and may contain information that is
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under applicable law. If the reader of this email is not the
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[Histonet] Microtome Service

[Fawn Bomar]

Does anyone know of any company that is able to service the Leica RM2155 
microtomes?  We are located in South Boston, Va.



Thank You



Fawn Bomar
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[Histonet] Microtome advise...

[Tom McNemar]

Hello all.  I am preparing my budget and am seeking recommendations on 
automated microtomes.  I am specifically interested in brands other than Leica 
(I already have two).  I would appreciate any thoughts you may have.  Thanks.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org


This e-mail, including attachments, is intended for the sole use of the 
individual and/or entity to whom it is addressed, and contains information from 
Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4641. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
arrive late or incomplete, or contain viruses. The sender therefore does not 
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Re: [Histonet] Microtome advise...

[Jack Ratliff]

How about the new Sakura with the auto orientation feature! I saw it on display 
at the Missouri Society for Histotechnology meeting and it was quite 
impressive! Of course if you have the budget, there's alway the non-contact 
laser microtome from Rowiak! I have seen and used that unit to cut fresh soft 
tissues (brain) and resin embedded bone and biomaterial implants!

Jack


On Jul 18, 2013, at 7:57 AM, Tom McNemar tmcne...@lmhealth.org wrote:

 Hello all.  I am preparing my budget and am seeking recommendations on 
 automated microtomes.  I am specifically interested in brands other than 
 Leica (I already have two).  I would appreciate any thoughts you may have.  
 Thanks.
 
 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
 www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org
 
 
 This e-mail, including attachments, is intended for the sole use of the 
 individual and/or entity to whom it is addressed, and contains information 
 from Licking Memorial Health Systems which is confidential or privileged. If 
 you are not the intended recipient, nor authorized to receive for the 
 intended recipient, be aware that any disclosure, copying, distribution or 
 use of the contents of this e-mail and attachments is prohibited. If you have 
 received this in error, please advise the sender by reply e-mail and delete 
 the message immediately. You may also contact the LMH Process Improvement 
 Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be 
 secure or error-free as information could be intercepted, corrupted, lost, 
 destroyed, arrive late or incomplete, or contain viruses. The sender 
 therefore does not accept liability for any errors or omissions in the 
 contents of this message, which arise as a result of e-mail transmission. 
 Thank you.
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Re: [Histonet] Microtome advise...

[Jack Ratliff]

BTW. I'm certain that the Sakura unit will be on display at the NSH S/C meeting 
in Providence, RI (20-25 Sept 2013) and I know for a fact that the laser 
microtome will be on display there as well because it will be demonstrated and 
used, along with a Leica rotary microtome for part of a workshop I am 
co-presenting with Bob Skinner (WS # 61 - A Detailed Examination of Working 
With Decalcified and Undecalcified Bone In Support of Preclinical and Clinical 
Research).

Jack


On Jul 18, 2013, at 8:55 AM, Jack Ratliff ratliffj...@hotmail.com wrote:

 How about the new Sakura with the auto orientation feature! I saw it on 
 display at the Missouri Society for Histotechnology meeting and it was quite 
 impressive! Of course if you have the budget, there's alway the non-contact 
 laser microtome from Rowiak! I have seen and used that unit to cut fresh soft 
 tissues (brain) and resin embedded bone and biomaterial implants!
 
 Jack
 
 
 On Jul 18, 2013, at 7:57 AM, Tom McNemar tmcne...@lmhealth.org wrote:
 
 Hello all.  I am preparing my budget and am seeking recommendations on 
 automated microtomes.  I am specifically interested in brands other than 
 Leica (I already have two).  I would appreciate any thoughts you may have.  
 Thanks.
 
 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
 www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org
 
 
 This e-mail, including attachments, is intended for the sole use of the 
 individual and/or entity to whom it is addressed, and contains information 
 from Licking Memorial Health Systems which is confidential or privileged. If 
 you are not the intended recipient, nor authorized to receive for the 
 intended recipient, be aware that any disclosure, copying, distribution or 
 use of the contents of this e-mail and attachments is prohibited. If you 
 have received this in error, please advise the sender by reply e-mail and 
 delete the message immediately. You may also contact the LMH Process 
 Improvement Center at 740-348-4641. E-mail transmissions cannot be 
 guaranteed to be secure or error-free as information could be intercepted, 
 corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. 
 The sender therefore does not accept liability for any errors or omissions 
 in the contents of this message, which arise as a result of e-mail 
 transmission. Thank you.
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[Histonet] microtome aligner

[Maria Mejia]

Hello,

Can anyone provide any additional information regarding microtome aligners?
Do they work?  I have never used or seen this particular tool, so which brand 
works 
best?  I know of only one company that sells a microtome aligner (universal 
type).
Any information any one can provide will be most appreciated.

Best
Maria Mejia
San Francisco, CA


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Re: [Histonet] microtome aligner

[Grantham, Andrea L - (algranth)]

They do work. I saw one at NSH some years ago and came home and had the guys in 
our shop make one for me exactly like the one I saw and it cost next to 
nothing. We bought the little round level at home depot.

Andi




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edumailto:algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] microtome aligner

[Goins, Tresa]

Yikes.  There is a lot of play getting that bubble in the middle of the 
universal aligner that may translate to large variations in alignment each 
time the thing is used.  The high cost is probably for the clamp, but if the 
bubble level is worth $1.50 the quality of the clamp doesn't matter.

Save your money and use a blank paraffin block.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Mejia
Sent: Wednesday, July 17, 2013 8:12 AM
To: HistoNet
Subject: [Histonet] microtome aligner

Hello,

Can anyone provide any additional information regarding microtome aligners?
Do they work?  I have never used or seen this particular tool, so which brand 
works best?  I know of only one company that sells a microtome aligner 
(universal type).
Any information any one can provide will be most appreciated.

Best
Maria Mejia
San Francisco, CA


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Re: [Histonet] microtome aligner

[Paula Sicurello]

I used an even cheaper version of the aligner.  My microtome service
engineer suggested an L level or a T level that you can buy at Home
Depot.  The L looks just like an L, it is actually a right angle measuring
device, used to make sure the corners or something are straight at 90
degrees.

You take the knife holder out and place one side of the L on the knife
holder place and the other against the block face.

Paula  :-)

-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091

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delete the original message.

On Wed, Jul 17, 2013 at 11:14 AM, Grantham, Andrea L - (algranth) 
algra...@email.arizona.edu wrote:

 They do work. I saw one at NSH some years ago and came home and had the
 guys in our shop make one for me exactly like the one I saw and it cost
 next to nothing. We bought the little round level at home depot.

 Andi




 Andrea Grantham, HT (ASCP)
 Senior Research Specialist
 University of Arizona
 Cellular and Molecular Medicine
 Histology Service Laboratory
 P.O.Box 245044
 Tucson, AZ 85724

 algra...@email.arizona.edumailto:algra...@email.arizona.edu
 Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] microtome aligner

[joelle weaver]

Yes you can certainly align the old fashioned way easy enough, but I find these 
are very handy and do save some time. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: algra...@email.arizona.edu
 CC: histonet@lists.utsouthwestern.edu
 Date: Wed, 17 Jul 2013 15:14:16 +
 Subject: Re: [Histonet] microtome aligner
 
 They do work. I saw one at NSH some years ago and came home and had the guys 
 in our shop make one for me exactly like the one I saw and it cost next to 
 nothing. We bought the little round level at home depot.
 
 Andi
 
 
 
 
 Andrea Grantham, HT (ASCP)
 Senior Research Specialist
 University of Arizona
 Cellular and Molecular Medicine
 Histology Service Laboratory
 P.O.Box 245044
 Tucson, AZ 85724
 
 algra...@email.arizona.edumailto:algra...@email.arizona.edu
 Tel: 520.626.4415 Fax: 520.626.2097
 
 
 
 
 
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[Histonet] Microtome repair

[Bruce Gapinski]

Jeff Myers is the best I've seen in my 35 years. This guy will cut beautiful 
sections as his final test after repair. He's one of us.
(408) 469-0957

Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF




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Re: [Histonet] Microtome upgrade, planning stages

[Maxim Peshkov]

I tried some different microtomes
(Leica, Microm, Thermo Shandon,
Sakura), as sledge as rotary types.
My choise is Leica RM2245.
Leica RM2245 is semi automatic? and
RM2255 or RM2265 are full automatic
models.
I like RM2245 because this model
have a rock mode, when operator
does for a section only 1/4 rotation.
It is very ergonomic!

Shandon Finesse ME also have rock
mode, but I have not happy with it.

Leica RM series have only one
disadvantage - two different type for
disposable blade holder (for low and high
profile, but not universal). Buy both
type holders.
RM2245 cheaper than 55 or 65.
Series 2125 is the same as Microm 325.

I do not try Leica Cool Clamp.

Hope this help.

Maxim Peshkov
Russia,
Taganrog.


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Re: [Histonet] Microtome upgrade, planning stages...

[Rene J Buesa]

Add another Leica to your list
René J,

From: kgrob...@rci.rutgers.edu kgrob...@rci.rutgers.edu
To: histonet histonet@lists.utsouthwestern.edu 
Sent: Thursday, March 7, 2013 2:32 PM
Subject: [Histonet] Microtome upgrade, planning stages...

We are starting to look into upgrading to higher-end, but used,
microtomes.  What we have is still working, but the writing is on the wall
for them in terms of repair/parts.

Which brands/models are considered higher-end in the used market these
days?  (Leica is already on my list, but which models?)

Thanks so much!

Kathleen


Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology  Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905

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[Histonet] Microtome blades

[Laurie Colbert]

Does anyone know who I can purchase Accu-edge blades from, other than Cardinal 
or American Master Tech?

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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Re: [Histonet] Microtome blades

[Rene J Buesa]

Sakura
René J.

From: Laurie Colbert lcolb...@pathmdlabs.com
To: Histonet Post (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu 
Sent: Wednesday, January 9, 2013 10:55 AM
Subject: [Histonet] Microtome blades

Does anyone know who I can purchase Accu-edge blades from, other than Cardinal 
or American Master Tech?

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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RE: [Histonet] Microtome blades

[Bartlett, Jeanine (CDC/OID/NCEZID)]

If you order them from Cardinal they get them from SakuraI think. Or maybe 
vice-versa...

Jeanine H. Bartlett
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, January 09, 2013 12:15 PM
To: Laurie Colbert; Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] Microtome blades

Sakura
René J.

From: Laurie Colbert lcolb...@pathmdlabs.com
To: Histonet Post (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu 
Sent: Wednesday, January 9, 2013 10:55 AM
Subject: [Histonet] Microtome blades

Does anyone know who I can purchase Accu-edge blades from, other than Cardinal 
or American Master Tech?

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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[Histonet] Microtome maintenance frequency

[Allen Keeping D .]

I have a question regarding scheduled maintenance of rotary microtomes. I run a 
simulation lab for students of lab technology. Part of their histology rotation 
involves learning how to produce acceptable sections on the microtome. I 
currently have 3 Leica rotary (manual) microtomes, which are used by students a 
total of 18-20 days per year (for educational purposes, not to produce 
diagnostic materials). In addition, I may use a microtome for a couple weeks 
total each year to produce control materials and to troubleshoot staining 
issues.

Currently, the microtomes are serviced by a Leica-educated professional 
annually for preventative maintenance. As these microtomes are only used 
infrequently, is this level of maintenance necessary (or recommended)? I am 
contemplating switching to a bi-annual schedule as a cost-saving measure.

Does anyone have any advice or experience regarding maintaining microtomes in a 
low-volume setting?

Cheers,

Allen



  
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Re: [Histonet] Microtome maintenance frequency

[Rene J Buesa]

For your usage level  it is unnecessary an annual maintenance. As a matter of 
fact I am sure you will be able to use those Leica microtomes for many years ar 
the same usage level without needing any capital maintenance.

René J.

From: Allen Keeping D. allen.keep...@albertahealthservices.ca
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu 
Sent: Thursday, December 27, 2012 1:40 PM
Subject: [Histonet] Microtome maintenance frequency

I have a question regarding scheduled maintenance of rotary microtomes. I run a 
simulation lab for students of lab technology. Part of their histology rotation 
involves learning how to produce acceptable sections on the microtome. I 
currently have 3 Leica rotary (manual) microtomes, which are used by students a 
total of 18-20 days per year (for educational purposes, not to produce 
diagnostic materials). In addition, I may use a microtome for a couple weeks 
total each year to produce control materials and to troubleshoot staining 
issues.

Currently, the microtomes are serviced by a Leica-educated professional 
annually for preventative maintenance. As these microtomes are only used 
infrequently, is this level of maintenance necessary (or recommended)? I am 
contemplating switching to a bi-annual schedule as a cost-saving measure.

Does anyone have any advice or experience regarding maintaining microtomes in a 
low-volume setting?

Cheers,

Allen



  
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recipient(s), are confidential and may contain privileged information. Any 
unauthorized review, use, retransmission, or other disclosure is strictly 
prohibited. If you have received this message in error, please notify the 
sender immediately, and then delete the original message. Thank you.
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RE: [Histonet] Microtome knives

[Patsy Ruegg]

There are knife sharpening services Sturkey and Dorn and Hart are two that
come to mind.  You can also find refurbished disposable blade holders and
buy disposable blades, the blade holder you get will determine if you use
low profile or high profile blades.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pru...@ihctech.net

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo
Sent: Sunday, November 11, 2012 2:45 AM
To: Jon Krupp; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome knives

I prefer to sharpen my microtome knives by myself by hand.
I have a vintage Cambridge Rocking Microtome and despite its age it works
very well.
Sharpening is a time consuming for the first time, it's depends on the
conditions of the blade edge.
Once you have a nice cutting profile its maintenance it's quite easy and it
takes a few minutes by
stroking the knife on a flat glass with oil and a bit of aluminium oxide
powder (3 -1 micron grits).
For me sharpening and honing of a microtome knife has became a secondary
hobby.
A solid knife has the advantage, compared to a disposable blade, to be
liable to less vibrations.

Kind Regards,
Massimo Tosi


A humble Chemical
Engineer who loves Histology






 Da: Jon Krupp jkr...@deltacollege.edu
A: Histonet@lists.utsouthwestern.edu 
Inviato: Venerdì 9 Novembre 2012 19:49
Oggetto: [Histonet] Microtome knives
 
Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did
all my sectioning in a plant research lab, but now I find myself needing to
learn more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need
advice about whether it would be better to find a knife sharpener and use
the microtome knives we have, or check into getting a disposable knife
holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see
references to high profile and low profile blades and holders, and I don't
know the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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Re: [Histonet] Microtome knives

[Massimo]

I prefer to sharpen my microtome knives by myself by hand.
I have a vintage Cambridge Rocking Microtome and despite its age it works very 
well.
Sharpening is a time consuming for the first time, it's depends on the 
conditions of the blade edge.
Once you have a nice cutting profile its maintenance it's quite easy and it 
takes a few minutes by
stroking the knife on a flat glass with oil and a bit of aluminium oxide powder 
(3 -1 micron grits).
For me sharpening and honing of a microtome knife has became a secondary 
hobby.
A solid knife has the advantage, compared to a disposable blade, to be liable 
to less vibrations.

Kind Regards,
Massimo Tosi


A humble Chemical
Engineer who loves Histology






 Da: Jon Krupp jkr...@deltacollege.edu
A: Histonet@lists.utsouthwestern.edu 
Inviato: Venerdì 9 Novembre 2012 19:49
Oggetto: [Histonet] Microtome knives
 
Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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RE: [Histonet] Microtome knives

[HARRISON,Sharon R]

Hi All,
I am Sharon Harrison, supervisor of Histopathology lab at UWI Mona in Kingston 
Jamaica.

I agree with you that we still need to have the solid blades for the cutting of 
hard tissue such as cervix fibroids and bone. Hence we have to maintain some 
vestige of the old technology of knife sharpening, as the disposable blades are 
quite expensive.

The disposable blades are great for biopsies and softer tissue but a real pain 
for getting sections of the harder tissues.

Therefore I advice having both systems especially if your laboratory handles 
all types of tissue specimen.
Sharon Harrison
Chief Medical Technologist
DMT, BSc, MPH




From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo 
[max_histo...@yahoo.it]
Sent: Sunday, November 11, 2012 4:44 AM
To: Jon Krupp; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome knives

I prefer to sharpen my microtome knives by myself by hand.
I have a vintage Cambridge Rocking Microtome and despite its age it works very 
well.
Sharpening is a time consuming for the first time, it's depends on the 
conditions of the blade edge.
Once you have a nice cutting profile its maintenance it's quite easy and it 
takes a few minutes by
stroking the knife on a flat glass with oil and a bit of aluminium oxide powder 
(3 -1 micron grits).
For me sharpening and honing of a microtome knife has became a secondary 
hobby.
A solid knife has the advantage, compared to a disposable blade, to be liable 
to less vibrations.

Kind Regards,
Massimo Tosi


A humble Chemical
Engineer who loves Histology






 Da: Jon Krupp jkr...@deltacollege.edu
A: Histonet@lists.utsouthwestern.edu
Inviato: Venerdì 9 Novembre 2012 19:49
Oggetto: [Histonet] Microtome knives

Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder.

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference.

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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[Histonet] Microtome knives

[Jon Krupp]

Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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Re: [Histonet] Microtome knives

[Rene J Buesa]

Because of your donated knives  you will have to buy a knives sharpener which 
are costly and not very easy to find.
Your best option is to buy a high profile disposable blades holder (that will 
be cheaper), buy disposable blades and avoid all the frustrations and waste of 
time sharpening knives.
René J.




From: Jon Krupp jkr...@deltacollege.edu
To: Histonet@lists.utsouthwestern.edu 
Sent: Friday, November 9, 2012 1:49 PM
Subject: [Histonet] Microtome knives

Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







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RE: [Histonet] Microtome knives

[Connolly, Brett M]

Hi Jon,

Depends on your cash flow. You could get a used sharpener somewhere off the web 
somewhere such as 
http://www.labx.com/v2/adsearch/resultsnew.cfm?sw=sharpenermr=25te=cat , or 
http://www.medwow.com/used-microtome-knife-sharpener-equipment/63.med but 
sharpening knives is a pain IMO and steel knives present more of a safety 
hazard. I would recommend a sharpener that uses the glass honing plates. You 
would also need the coarse and fine abrasives.

Personally, I would opt for a low profile disposable blade holder that fits 
your 820. Low and high profile refer to the size (height) of the blade. We use 
low profile for paraffin block sectioning and high profile for cryostat 
sectioning.

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jon Krupp
Sent: Friday, November 09, 2012 1:50 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome knives

Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

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Electron Microscopy at SJ Delta College







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Re: [Histonet] microtome

[E. Wayne Johnson]

I got it open and cleaned the wheel stop mechanism and
the belt in front and it works good now and we now have a backup and
my people are smiling.

Thanks for everyone's helpful advices.

Wayne Johnson
Enruikang (Enable) Ag Tech
Beijing

On 9/22/2012 4:34 AM, Burton, Lynn wrote:

Perhaps they have a contact person since they do so much work throughout the US.

From: jsjurc...@comcast.net [mailto:jsjurc...@comcast.net]
Sent: Friday, September 21, 2012 3:16 PM
To: Jay Lundgren
Cc: Histonet; Burton, Lynn
Subject: Re: [Histonet] microtome

As I said, I'm not sure they have anybody in Beijing.

From: Jay Lundgrenjaylundg...@gmail.com
To: Lynn Burtonlynn.bur...@illinois.gov
Cc: Histonethistonet@lists.utsouthwestern.edu
Sent: Friday, September 21, 2012 2:27:59 PM
Subject: Re: [Histonet] microtome

Um.he is in Beijing.
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[Histonet] microtome

[E. Wayne Johnson]

We work in a previously underutilized histology lab.

There was a sliding horizontal Sakura microtome, a functioning Thermo 
microtome,

and a nonfunctioning Leica 5xxx (not a very fancy Leica).

The center where we work has no interest in fixing the Leica microtome.
I am supposing that it is merely dirty.  Beijing is very dusty and the 
windows to

the room leaked badly until we installed window seals.

I would like to try to take the Leica microtome apart and try to clean 
it but I cant find
the way to get it open.  There is no hatch to open like on an old AO 
Spencer.


We are told that it would cost way to much to have Leica service it.

Does anyone have any experience with maintenance or repair of a low-end 
Leica Microtome

and have any tips to share on how to open and clean it?

Wayne Johnson
Beijing.

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Re: [Histonet] microtome

[E. Wayne Johnson]

I think its a Leica 2125.

On 9/22/2012 12:32 AM, E. Wayne Johnson wrote:

We work in a previously underutilized histology lab.

There was a sliding horizontal Sakura microtome, a functioning Thermo 
microtome,

and a nonfunctioning Leica 5xxx (not a very fancy Leica).

The center where we work has no interest in fixing the Leica microtome.
I am supposing that it is merely dirty.  Beijing is very dusty and the 
windows to

the room leaked badly until we installed window seals.

I would like to try to take the Leica microtome apart and try to clean 
it but I cant find
the way to get it open.  There is no hatch to open like on an old AO 
Spencer.


We are told that it would cost way to much to have Leica service it.

Does anyone have any experience with maintenance or repair of a 
low-end Leica Microtome

and have any tips to share on how to open and clean it?

Wayne Johnson
Beijing.

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