I also noticed stain fading with time - controls cut way ahead of staining?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com
On 1/23/15, 1:00 PM, Wheelock, Timothy R. twheel...@mclean.harvard.edu
wrote:
Hi Jeffery:
Yes, I notice the same paraffin dust bunnies, perhaps especially since
I use a embedding paraffin that has a fair amount of plastic.
Before I embed my brain tissue, I mix the embedding media thoroughly,
until the solution is clear.
As for your Congo Red problem, I wish that I could help you.
I am now experiencing the same problem, except that it affects the
positive controls as well.
The staining is there, but much fainter than it should be.
I usually immerse albumin coated slides in 1% Congo red for 15 minutes,
differentiate them with Potassium Hydroxide for 3 dips, counterstain with
Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10
dips, immerse in running tap water for 10 minutes, immerse in 95% ethanol
for 2 dips, then dehydrate and mount.
Tim
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