Re: [Histonet] Weigert's hematoxylin for IHC

[Rene J Buesa via Histonet]

Once you finish the IHC procedure, the DAB reaction is very stable and you can 
use Weigert's or any other iron hematoxylin.René 

On Tuesday, November 22, 2016 5:29 PM, Esther C Peters via Histonet 
 wrote:
 

 Could someone advise me on whether Weigert's iron hematoxylin can be used as 
the counterstain for nuclei in IHC? We need to avoid using Harris's hematoxylin 
because that will stain mucocytes in the coral tissues we are working with. If 
it can be used, would that be after all antibody steps have been done? Thank 
you!


Esther Peters


Esther C. Peters, Ph.D.
Term Associate Professor
Environmental Science & Policy
George Mason University
4400 University Drive, MS 5F2
Fairfax, VA 22030-
Office: David King Hall, Room 3050
Phone: 703-993-3462
Fax: 703-993-1066
e-mail: epete...@gmu.edu
http://esp.gmu.edu

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Re: [Histonet] Weigert's hematoxylin for IHC

[Caroline Miller via Histonet]

I would caution using Weigerts as a counterstain for IHC because it is
pretty dark in color and could be confused with the brown of the staining
you are looking for in your IHC. Have you tried Mayer's? That often
provides a pleasing stain with minimal effort.

You would counterstain after the DAB development steps, prior to mounting
the slides with coverslips.

I have never seen coral staining though, so this is only a guess from what
works in regular mammalian tissues, good luck!

yours,
mills

On Tue, Nov 22, 2016 at 2:24 PM, Esther C Peters via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Could someone advise me on whether Weigert's iron hematoxylin can be used
> as the counterstain for nuclei in IHC? We need to avoid using Harris's
> hematoxylin because that will stain mucocytes in the coral tissues we are
> working with. If it can be used, would that be after all antibody steps
> have been done? Thank you!
>
>
> Esther Peters
>
>
> Esther C. Peters, Ph.D.
> Term Associate Professor
> Environmental Science & Policy
> George Mason University
> 4400 University Drive, MS 5F2
> Fairfax, VA 22030-
> Office: David King Hall, Room 3050
> Phone: 703-993-3462
> Fax: 703-993-1066
> e-mail: epete...@gmu.edu
>  xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPP
> KrrDjhwOvk.=http%3a%2f%2fesp.gmu.edu>http://esp.gmu.edu
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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[Histonet] Weigert's hematoxylin for IHC

[Esther C Peters via Histonet]

Could someone advise me on whether Weigert's iron hematoxylin can be used as 
the counterstain for nuclei in IHC? We need to avoid using Harris's hematoxylin 
because that will stain mucocytes in the coral tissues we are working with. If 
it can be used, would that be after all antibody steps have been done? Thank 
you!


Esther Peters


Esther C. Peters, Ph.D.
Term Associate Professor
Environmental Science & Policy
George Mason University
4400 University Drive, MS 5F2
Fairfax, VA 22030-
Office: David King Hall, Room 3050
Phone: 703-993-3462
Fax: 703-993-1066
e-mail: epete...@gmu.edu
http://esp.gmu.edu

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