[Histonet] Question on Sanderson Rapid Bone Stain

Hello everyone, I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain. My first attempt at this stain resulted in a very faint stain from the RBS (I could see very fa

[Histonet] Villanueva Osteochrome Bone Stain on already embedded ground sections

Hello everyone, I was just wondering if anyone has a protocol for staining undecalcified bone already embedded in poly(methyl methacylate) using the Villanueva Osteochrome Bone Stain? I have 30-40 micron thick samples and I cannot deplasticize my samples as suggested by the manufacturer's pro

[Histonet] Buffer concentration in 4% paraformaldehyde

Hello everyone, I was wondering if anyone knows if there is a difference between preserving tissue (bone to be specific) in 4% paraformaldehyde made with 10x phosphate buffer versus 1x phosphate buffer? We typically use 1x phosphate buffer when making 4% paraformaldehyde, but have some sampl