The optimal temperature for most enzymes in 37 degrees Celsius.
From: "Lager, Loree via Histonet"
To: "'histonet@lists.utsouthwestern.edu'"
Date: 11/03/2015 12:38 PM
Subject:[Histonet] Pas Digestion
Hello,
This is my first time using the histonet, as our veteran user ret
In one of our classes the students do IHC staining by hand. They perform
multiple procedures over the semester. We currently use Biocare reagents.
The polymer based detection systems are simple to use. The data sheets
that accompany the antibodies and detection reagents are really helpful
w
Akemi,
I think this offer is low for a certified HT/HTL, particularly in that
area. I have had graduates go up to Northern CA and the minimum starting
wage that they have accepted is $25. These are brand new graduates, with
minimal experience, but I don't believe their titles are "trainees".
J
Looking for the contact information for Leica sales in Southern
California.
Thanks,
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Do you using "freezing spray" on these specimens? Direct spray can cause
the tissue to appear burnt.
From: Charles Riley via Histonet
To: histonet@lists.utsouthwestern.edu
Date: 01/05/2016 06:00 AM
Subject:[Histonet] Processing of breast tissue
We have been experiencing so
There are a couple of things that it might be.
1. Uneven deparaffinization before staining.
2. Water in your last reagents/paraffin on the processor.
From: mohamed abd el razik via Histonet
To: "Histonet@lists.utsouthwestern.edu"
Date: 01/26/2016 11:33 AM
Subject:[Histone
There is no need to be rude. He has tried the drying option and is still
having nuclear bubbling. He is exploring other possible issues. You
would see this if you read the email in its entirety.
From: Rene J Buesa via Histonet
To: "Vickroy, James" ,
"histonet@lists.utsouthwestern.e
There is a reference to erythrosin in Lynch's Medical Laboratory
Technology, but it is brief. Culling is the author of the histology
chapter, so perhaps he has something more in depth.
From: "Tony Henwood (SCHN) via Histonet"
To: "histonet@lists.utsouthwestern.edu"
Date: 04/03/201
In addition to the Histonet, I am on a listserve for clinical laboratory
educators. Dr. Raff posts his blog link on that listserve as well. He
also responds to some of the questions. There has never been one public
complaint regarding his posts. It is very clear in the subject line and
the
I'm told that it is the ammonia in urine that is the "active ingredient".
Perhaps trying ammonia water?
From: "deGuzman, Jose R via Histonet"
To: "histonet@lists.utsouthwestern.edu"
Date: 04/27/2016 01:58 PM
Subject:Re: [Histonet] Sea urchin Spines
Have you tried urine or
We switched form malt diastase to alpha amylase and have seen a
significant improvement in digestion, but as others have said enzyme
activity will be destroyed at 60 degrees C. We put ours into a 37 degree C
water bath. Enzymes work optimally at 37C, body temperature. As the
temperature rises
clse...@list.apsu.edu
This is a list serve for MT/MLT Educators.
Sent from my iPhone
> On May 23, 2016, at 6:09 AM, Pam Barker via Histonet
wrote:
>
> Hi Histonetters!
> I hope everyone is looking forward to a great week and a fantastic
Memorial
> Day Weekend. I am hoping someone can help me w
0.5 grams in 400 mL is 0.125%. Dilute your 1.3% tenfold and it will be
close enough.
From: Amy Johnson via Histonet
To: "histonet@lists.utsouthwestern.edu"
Date: 06/13/2016 10:47 AM
Subject:[Histonet] Powder Picric Acid vs Liquid Picric Acid
Our current procedure for the
are you doing a microwave method or traditional?
From: Jeff Halstead via Histonet
To: "histonet@lists.utsouthwestern.edu"
Date: 06/16/2016 10:17 AM
Subject:[Histonet] warthin-starry stain
Hello histonet---I am a tech in western Oregon, and I have been
experiencing strange
Heat can cause poor chromatin staining. Is the two shades of eosin only
on the biopsies, or all tissues?
From: Charles Riley via Histonet
To: histonet@lists.utsouthwestern.edu
Date: 06/16/2016 11:19 AM
Subject:[Histonet] Poor Staining
Can over processing small biopsies lea
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Lecia makes the Sakura microtome. We have both Sakura and Microm
microtomes in our student lab. They have both proved to be reliable.
There is a difference between a Shandon microtome and a Microm microtome.
From: Rene J Buesa via Histonet
To: Mary Faith Encarnacion ,
"histonet@list
Your ignorant sarcasm isn't appreciated. Leica makes the microtome and
private labels it for Sakura.
Sent from my iPhone
> On Aug 2, 2016, at 7:46 AM, Rene J Buesa via Histonet
wrote:
>
> As far as I know Leica Microsystems has bought/absorbed along the years
many optical/instruments makers, su
Elizabeth,
You can try to lower the pH of the eosin a bit. Add some acetic acid. Use
absolute alcohol after the eosin for differentiating/dehydrating.
Jennifer
From: "Cameron, Elizabeth via Histonet"
To: "histonet@lists.utsouthwestern.edu"
Date: 08/30/2016 07:29 AM
Subject:
This decision could affect histology labs as well. Please read below and
consider the effects of having a nurse in charge of the lab.
Jennifer
Petition Urging CMS to Reconsider Ruling on Nursing Degree Equivalence
ASCLS, in conjunction with our partners on the Board of Certification,
urge the
This may be a better option:
https://www.labce.com/histology_exam_simulator.aspx
The NSH has partnered with media lab for exams. You have a full year
access to the questions. There are over 2,000 questions in the question
bank and there are images as well. NSH members pay the discounted rate
Fawn,
There is no specification as to what type of degree it must be. It can be
any baccalaureate degree as long as you have at least 30 semester hours of
biology and chemistry combined. I have had students with art and
psychology degrees sit for the HTL exam. They came back to college to get
We cut ours at 8 um within a couple of days of when we are going to use
them. We bake them as usual.
From: "Myles, Irene via Histonet"
To: "'histonet@lists.utsouthwestern.edu'"
Date: 11/14/2016 12:26 PM
Subject:[Histonet] Congo Red Controls
Good Afternoon Histonet,
How d
The California Society for Histotechnology will be hosting the 41st Annual
Symposium in San Jose May 19-21 at the Hilton. We are accepting abstracts
for workshops at this time.
Although it is the 41st we will be celebrating our 40th.
Please email me with your abstract.
jmacdonald...@gmail.com
This is a great opportunity for those teaching histotechnology. Although
many of the workshops are CLS oriented there are many that are about
teaching laboratory science in general. There are also workshops about
clinical rotation issues.
I have gone several years and always bring back great i
I respectively disagree with this analogy. Eggnog is dairy based, made
with milk, heavy cream and sugar. Dairy products have a much shorter life
than egg whites.
From: Rene J Buesa via Histonet
To: Nancy Schmitt ,
"'histonet@lists.utsouthwestern.edu'"
Date: 04/12/2017 09:07 AM
Su
Tim,
I have this method in one of medical technology books from many years ago.
Lynch's Medical Laboratory Technology II. The author of the Histology
section was Charles F. A. Culling. There are two procedures, one for
urine and one for nervous system tissue. I assume you want the nervous
s
The California Society for Histotechnology is hosting their annual
symposium May 18-21 in San Jose. Please join us for some great
workshops and California spring weather. San Jose is a great airport
to fly into.
More information can be found at:
http://californiahistology.org/e
It's not too late to register for the 2017 CSH 40th Annual symposium. Lots
of great workshops!
No late fees!
http://californiahistology.org/events.html#csh2017
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We have two of the Paratrimmers by Shandon. They really cut down on the
mess with scraping the blocks and the repetitive motion.
From: "Underwood, Fred via Histonet"
To: "histonet@lists.utsouthwestern.edu"
Date: 05/24/2017 06:04 AM
Subject:[Histonet] heated block trimmers
For many years we used ClearRite, both on our tissue processor and for
staining. We are now using a similar product from another company. We
did not see any problems with our IHC with the use of this product. We do
not do molecular testing. We found it to be far superior to the limonenes
an
Is anyone familiar with digital scanning microscopes? We are in the
market for one.
Thank you.
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The HM325 also has that feature.
From: Jay Lundgren via Histonet
To: Julio Benavides
Cc: histonet
Date: 09/01/2017 10:11 AM
Subject:Re: [Histonet] comparison between microtomes leica RM
2125RTS y thermo HM325
I prefer the Leica, because it has that nifty rough cut but
Hi All,
The California Society for Histotechnology will have its annual symposium
in Anaheim, CA May 4-6, 2018. If you are interested in presenting please
send me your abstract. Thank you,
Jennifer
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I have a colleague looking for a lab that can handle about 400 specimens
for H&E staining. Any recommendations?
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Thank you to all that responded. I have a little more information about
the project.
The specimens are subdermal implants in mouse. The specimens are
cylinders about 5 mm long. The first student has about 400 specimens and
there may be more to follow. The tissue has been fixed in formalin
We were able to find a local lab that experience with the specimens in
question. Thank you to all that responded with offers of help.
Thank you,
Jennifer MacDonald
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