[Histonet] Warthin starry

Is there any way to lighten and over stainer WS stain? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] warthin-starry

Hi all thanx for the tips-I am not really allowed to use acid washes-don't have access to a reliable microwave-the stain is done by hand with me assimilating the different reagents-or a mastertech kit. The idea of the bleach causing problems is interesting-before I was told not to use the stron

Re: [Histonet] warthin-starry stain

are you doing a microwave method or traditional? From: Jeff Halstead via Histonet To: "histonet@lists.utsouthwestern.edu" Date: 06/16/2016 10:17 AM Subject: [Histonet] warthin-starry stain Hello histonet---I am a tech in western Oregon, and I have been experienci

[Histonet] warthin-starry stain

Hello histonet---I am a tech in western Oregon, and I have been experiencing strange problems with the afore mentioned stain. When the stain is run a very strange grey precipitate deposits upon the slides. The deposition is not uniform across the slides and varies from slide to slide within the

[Histonet] Warthin Starry "snowflakes"

We are have been trying to troubleshoot our Warthin Starry stain. We have been seeing "snowflakes" appearing on the slides. The pathologists sometimes have a hard time as they sort of look like they could be spirochetes. Please help!! Thanks! Rhonda Gregoire, MLT Supervisor, Clinical Patholog

RE: [Histonet] Warthin Starry on Dako Artisan

5:13 AM To: Cooper, Brian; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Warthin Starry on Dako Artisan What protocol with uranyl nitrate do you use? Dako told us to use zinc formalin as a sensitizer but it doesn't seem to make much of a difference. Thanks! -O

RE: [Histonet] Warthin Starry on Dako Artisan

'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Warthin Starry on Dako Artisan What protocol with uranyl nitrate do you use? Dako told us to use zinc formalin as a sensitizer but it doesn't seem to make much of a difference. Thanks! -Original Message

RE: [Histonet] Warthin Starry on Dako Artisan

lf Of Rene J Buesa Sent: Tuesday, December 23, 2014 11:18 AM To: Cooper, Brian; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry on Dako Artisan Try a 0.10% solution of phosphotungstic acid.René J.  On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" wro

Re: [Histonet] Warthin Starry on Dako Artisan

Try a 0.10% solution of phosphotungstic acid.René J.  On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" wrote: Happy Holidays Histonetters! Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with

[Histonet] Warthin Starry on Dako Artisan

Happy Holidays Histonetters! Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate? With offline uranyl nitrate pretreatment, spirochetes stain brilliantly! But everything else we've tried leav

RE: [Histonet] Warthin Starry Help

MacDonald; Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Warthin Starry Help My hydroquonine was received in the lab in 1980 and it works. As long as my control is positive I am allowed to keep it but then I work in a veterinary diagnostic lab and am not under all

[Histonet] warthin starry problems

Dear Histonetters: Here is a message I am posting from Tasha...she hasn't yet joined the list but is eager for answers to her question. I will forward answers to her or you can send them directly to her at tmcampb...@fmh.org "I need help with warthin starry stain pl

RE: [Histonet] Warthin Starry Help

: [Histonet] Warthin Starry Help Hydroquinone has a short shelf life once opened. > On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." wrote: > > I need help with warthin starry stain please. I am a very small lab > trying to save money so I want to make the reagents myself. I

RE: [Histonet] Warthin Starry Help

14, 2014 10:16 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry Help Hydroquinone has a short shelf life once opened. > On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." wrote: > > I need help with warthin starry stain please.

Re: [Histonet] Warthin Starry Help

Hydroquinone has a short shelf life once opened. > On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." wrote: > > I need help with warthin starry stain please. I am a very small lab > trying to save money so I want to make the reagents myself. I have > sigma Aldrich dry reagents and have been ma

Re: [Histonet] Warthin Starry Help

for preparing your own reagents. You belong to a "dying breed" of histotechs. René J. From: "Campbell, Tasha M." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 14, 2014 9:55 AM Subject: [Histonet] Warthin Starry Help I ne

[Histonet] Warthin Starry Help

I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first sta

[Histonet] Warthin starry fading

Hi Gudrun I had this same problem some years back. I changed from a previous mounting medium to Merck Entellan New. The H&E's were fine but when I did warthin-starry staining they were completely faded the next day. I thought it must be the new mounting medium so I bought some Pertex and ran 2 c

AW: [Histonet] warthin starry fading

n.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Donnerstag, 15. September 2011 17:17 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] warthin starry fading Hi all! Today I stained Warthin Starry for Hp. This time I was lucky to have the first

[Histonet] warthin starry fading

Hi all! Today I stained Warthin Starry for Hp. This time I was lucky to have the first time purchased control tissue (Spirochetes control from Sigma) for comparison. In the package there was also a ready stained slide with really nice black spirochetes in yellow-brownish tissue/nuclei. This was s

[Histonet] Warthin Starry Stain

Greetings! Our usual reference lab does not do a spirochete stain. I would appreciate contact information for a lab that can do a Warthin Starry on FFPE tissue. Thanks. Dorothy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://

RE: [Histonet] Warthin-Starry Stain

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 31, 2010 10:55 AM To: histonet@lists.utsouthwestern.edu; Chakib Boussahmain Subject: Re: [Histonet] Warthin-Starry Stain Because of intrinsic difficulties and no special advantages, I switched

Re: [Histonet] Warthin-Starry Stain

lways good results, instead of struggling with a technique that eventually will delay the slides to be presented to the pathologist. René J. --- On Tue, 8/31/10, Chakib Boussahmain wrote: From: Chakib Boussahmain Subject: [Histonet] Warthin-Starry Stain To: histonet@lists.utsouthwestern.edu

RE: [Histonet] Warthin-Starry Stain

Sarah Goebel, B.A., HT (ASCP) Histotechnician <= /em> XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 1= 00 Austin, Texas 78744 (512)386-5107 Original Message ---- Subject: [Histonet] Warthin-Starry Stain From: Chakib Boussahmain <

RE: [Histonet] Warthin-Starry Stain

@lists.utsouthwestern.edu Subject: [Histonet] Warthin-Starry Stain Hi Histonet, Does anyone out there uses Warthin-Starry Stain, If so, Can you help me with troubleshooting, my slides get overstained even though i respect the developing time that is in the procedure which is 3.5min. thank you in

[Histonet] Warthin-Starry Stain

Hi Histonet, Does anyone out there uses Warthin-Starry Stain, If so, Can you help me with troubleshooting, my slides get overstained even though i respect the developing time that is in the procedure which is 3.5min. thank you in advance and your help will be very much appreciated! Chakib HTL(ASC

RE: [Histonet] Warthin-Starry // "Best before" dates

On Behalf Of V. Neubert Sent: Tuesday, November 25, 2008 2:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin-Starry // "Best before" dates Hello! I use the Warthin-Starry technique to stain spirochaetes in FFPE sections. I don't know how long I can kee

[Histonet] Warthin-Starry // "Best before" dates

Hello! I use the Warthin-Starry technique to stain spirochaetes in FFPE sections. I don't know how long I can keep the AgNO_3 solutions and the hydrochinone solution whitout getting them to "expire". Is there anyone who already had to deal with that question? Regards, Valentin _