Re: the absorbance value 1.05 at 260/280 of protein solution

Hi all Thank you very much for the replies. On Wed, Oct 28, 2015 at 7:40 PM, Nair, Jayakumar < r.jayaku...@roswellpark.org> wrote: > Nothing if you are measuring only protein concentration. A Lot if you > were measuring DNA concentration. > Jay > > > -Original Message- > From:

RE: the absorbance value 1.05 at 260/280 of protein solution

Nothing if you are measuring only protein concentration. A Lot if you were measuring DNA concentration. Jay -Original Message- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of Sudheer Sangeetham Sent: Wednesday, October 28, 2015 7:14

RE: the absorbance value 1.05 at 260/280 of protein solution

You can use the A260/A280 as an indicator of protein purity. Ideally, pure protein should have an A260/A280 value of 0.6, so 1.05 is very high, and indicates that you have nucleic acid contamination (the A260 component is too high). From:

Re: the absorbance value 1.05 at 260/280 of protein solution

It means you have to get a good text book on spectrophotometry and read it. That will give you a good idea of what exactly it means. :) Good luck! Pow On 28 October 2015 at 07:13, Sudheer Sangeetham wrote: > Hello people > > What does it mean by the absorbance