Hi all,
I end up in the same problem when installing TPP. The Apache error log
looks like below. I have re-installed pearl, both the recomended
ActivePerl 5.8.9.826 MSI version and the latest. Looks like port 80 is
occupied by something else. I am trying to figure out with what.
Installing the
Hi all,
Thanks a lot for you advices.
Here I post the output for running the peptideProphet at the command
line:
C:\Inetpub\wwwroot\ISB\data\probidoutput\ipimousexinteract -
Ninteract.pep.xml
-p0.05 -l7 -OAFm -I2 -I3 -I4 -I5 -I6 -I7 analysis100.xml
analysis125.xml analy
sis150.xml
Hi Ben,
50gb for one file? That's impressive. That could be much improved with
compression (which the Agilent MH format also does), but since you need
to peak pick the MSn to identify them in most search engines, try this
command:
msconvert source.d --mzXML --filter peakPicking true [2,2]
Hi Melody,
I've tracked down and fixed the bug using the files you sent. My new
code is in the sashimi subversion code repository. Maybe someone can
build the PeptideProphetParser executable on windows and share that
with you until a new release is made available that includes this fix.
MassHunter does it if you pass 'true', ProteoWizard uses a basic local
maximum algorithm if you pass 'false'.
-Matt
Ben Collins wrote:
Hi Matt,
Thanks, this seems to have done the trick. I now have a 14 GB mzXML
file instead of 50 GB.
I am interested to know whether the peak picking is
We are testing algorithms with a yeast dataset from Peptide Atlas,
accession number PAe000145, contributed by Steven Gygi et al.
(Harvard). This entry does not list any related publications. Is there
any annotation that describes this dataset? We have already tried
contacting Dr. Gygi.
Thanks,
