Re: [spctools-discuss] running ASAPratio for SILAC data

2023-12-04 Thread 'David Shteynberg' via spctools-discuss
Of course, I am glad to help. The old and the new versions should run faster on the centroided data. There is no way to tell *a priori* with ASAPRatio how long it will take, but since it ran to completion on my machine and it generates one dot per ten PSMs it has processed; based on the dots you

Re: [spctools-discuss] running ASAPratio for SILAC data

2023-12-04 Thread sudarshan kumar
Thank you so much David for doing this for me. In fact I was running asapratio again on the centroid mzml file. But I see that though express completed easily but the asapratio is still running. Is there any way in tpp to see how much time will it take to complete. But I will try with the version

Re: [spctools-discuss] Hi I cannot access the gui anymore is there something I'm missing

2023-12-04 Thread David Shteynberg
Hello, If you could provide some additional information such as: your operating system, which version you installed and relevant configuration during installation, that would be helpful in troubleshooting your issue. Thanks! -David > On Dec 4, 2023, at 2:21 PM, Aarthie Senathirajah >

Re: [spctools-discuss] running ASAPratio for SILAC data

2023-12-04 Thread 'David Shteynberg' via spctools-discuss
Hello Sudarshan, again! I was able to update the ASAPRatioPeptideParser.exe tool for your testing purposes to see if it runs a bit faster after some optimization I was able to implement. Please download the following version:

[spctools-discuss] Re: Hi I cannot access the gui anymore is there something I'm missing

2023-12-04 Thread Aarthie Senathirajah
Hi I keep clicking on the TPP link and I get the same problem. Is there some file that I am missing? http://localhost:10401/tpp Any help would be appreciated On Friday, December 1, 2023 at 3:49:51 PM UTC-5 Aarthie Senathirajah wrote: > This site can’t be reached > > *localhost* refused to

Re: [spctools-discuss] running ASAPratio for SILAC data

2023-12-04 Thread 'David Shteynberg' via spctools-discuss
Hopefully, I am understanding your queries correctly, here goes. Centroiding the data will reduce the number of peaks in the spectra and reduce the running time of the analysis. Compressing the files to make them smaller will require that the tools reading the files uncompress the data (usually

Re: [spctools-discuss] running ASAPratio for SILAC data

2023-12-04 Thread sudarshan kumar
one more query- I used same samples - for label free and SILAC express? Ideally I should get a similar conclusion from both the experiments. But the differentially expressed proteins are different in the two analyses. NOte: I have used only one run file of control and one run file of treatment

Re: [spctools-discuss] running ASAPratio for SILAC data

2023-12-04 Thread sudarshan kumar
Hi David, I am using this tutorial for Expressan dASAPratio . can yuo please comment on if I am using the right one.? Regards, Sud On Thu, Nov 30, 2023 at 10:53 AM sudarshan kumar wrote: > can i select all the three options? > Will it impact Express analysis? > Centroid all scans (MS1 and MS2)