Re: [spctools-discuss] why N-terminus protonated for dimethyl label on ASAPratio?

2011-01-11 Thread David Shteynberg
The total mass of the modified amino-acid / terminus is specified to ASAPRatio. The unmodified n-terminus of any peptide is 1.007825 (or the mass of H), since your termini are modified the mass of the modification will be added to 1.0078. Also, please update to the latest version of the TPP (4.4

[spctools-discuss] why N-terminus protonated for dimethyl label on ASAPratio?

2011-01-11 Thread gkayihan
Hi all, I have been trying to quantify our dimethyl labeled samples for the last week using TPP. I got it to work when I ran heavy and light label SEQUEST searches separate and in ASAP ratio I chose the option of "static modification quantification" and chose K and n-terminus as the "labeled residu