Re: [spctools-discuss] MS3 quantification

2018-06-18 Thread chanyeric
Thanks, Jimmy. Libra has completed successfully with the new mzXML files. I did have to follow your earlier instructions and first get MSFileReader3.0 before running ReAdW: http://proteomicsresource.washington.edu/protocols06/wine/ -Eric On Monday, June 18, 2018 at 2:48:31 PM UTC-4, Jimmy Eng

Re: [spctools-discuss] MS3 quantification

2018-06-18 Thread Jimmy Eng
Eric, Unfortunately the Libra's MS3 TMT support is based on an optional "filterLine" attribute in the mzXML file that msconvert does not write out as far as I'm aware. Libra uses the information in the filter line to identify the ms3 scan number associated with the ms2 scan. As a workaround,

Re: [spctools-discuss] MS3 quantification

2015-07-07 Thread hh1985
Cool! I will try that. Thank you so much, Jimmy! -Han On Tuesday, July 7, 2015 at 11:52:33 AM UTC-4, Jimmy Eng wrote: Libra, the TPP's tool for quantifying TMT/iTRAQ data, will now work with MS3 quantification. Make sure you're using the latest TPP and have this setting in your Libra

Re: [spctools-discuss] MS3 quantification

2015-07-07 Thread Jimmy Eng
Libra, the TPP's tool for quantifying TMT/iTRAQ data, will now work with MS3 quantification. Make sure you're using the latest TPP and have this setting in your Libra condition file: reporterFromMS3 value=1 / On Tue, Jul 7, 2015 at 8:19 AM, hh1985 hh.earlyd...@gmail.com wrote: Hi all, I