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Journal of Economic Entomology 101(4):1389-1396. 2008

> <http://www.bioone.org/doi/abs/10.1603/0022-0493%282008%29101%5B1384%3ATOFAST%5D2.0.CO%3B2>
>
> *Ability of Bed Bug-Detecting Canines to Locate Live Bed Bugs and Viable
> Bed Bug Eggs*
>
> Margie 
> Pfiester1<http://www.bioone.org/doi/abs/10.1603/0022-0493%282008%29101%5B1389%3AAOBBCT%5D2.0.CO%3B2?journalCode=ecen#FN1>,
> Philip G. Koehler, Roberto M. Pereira
>
> Department of Entomology, Building 970 Natural Area Drive, University of
> Florida, Gainesville, FL 32611–0620
>
>  1Corresponding author, e-mail: [email protected].
>  Abstract
>
> The bed bug, *Cimex lectularius* L., like other bed bug species, is
> difficult to visually locate because it is cryptic. Detector dogs are useful
> for locating bed bugs because they use olfaction rather than vision. Dogs
> were trained to detect the bed bug (as few as one adult male or female) and
> viable bed bug eggs (five, collected 5–6 d after feeding) by using a
> modified food and verbal reward system. Their efficacy was tested with bed
> bugs and viable bed bug eggs placed in vented polyvinyl chloride containers.
> Dogs were able to discriminate bed bugs from *Camponotus floridanus*Buckley,
> *Blattella germanica* (L.), and *Reticulitermes flavipes* (Kollar), with a
> 97.5% positive indication rate (correct indication of bed bugs when present)
> and 0% false positives (incorrect indication of bed bugs when not present).
> Dogs also were able to discriminate live bed bugs and viable bed bug eggs
> from dead bed bugs, cast skins, and feces, with a 95% positive indication
> rate and a 3% false positive rate on bed bug feces. In a controlled
> experiment in hotel rooms, dogs were 98% accurate in locating live bed bugs.
> A pseudoscent prepared from pentane extraction of bed bugs was recognized by
> trained dogs as bed bug scent (100% indication). The pseudoscent could be
> used to facilitate detector dog training and quality assurance programs. If
> trained properly, dogs can be used effectively to locate live bed bugs and
> viable bed bug eggs.
>
>

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