I'm a very new user to this analysis tool, could anyone please help??
When trying to convert RAW to pepXML I keep getting this message for
each RAW file. Could this be because I don't have Xcaliber or Bioworks
on my laptop? I've had to use my laptop as didn't have the admin
priviledges on our labs computer

Thanks in advance,

Elisabeth (PhD student)


ReAdW -v --mzXML c:/Inetpub/wwwroot/ISB/data/SILAC/RAW/
elisabeth_3nov_08_01.RAW
--------------------------------------------------------------------------------

Settings:
  centroid scans: 0
  compress scans: 0
  verbose mode: 1
  lockspray: 1
  MS^E: 0
  threshold: 0
  mzML mode: 0
  mzXML mode: 1
  input filename: c:/Inetpub/wwwroot/ISB/data/SILAC/RAW/
elisabeth_3nov_08_01.RAW
  output filename: c:/Inetpub/wwwroot/ISB/data/SILAC/RAW/
elisabeth_3nov_08_01.mzXML

Unable to initialize Xcalibur 2.0 interface; falling back to 1.4
interface
Unable to initialize XCalibur 1.4 interface; check installation
hint: try running the command "regsvr32 C:\<path_to_Xcalibur_dll>
\XRawfile2.dll"
unable to interface with Thermo library
Command FAILED

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