Hello Elisabeth, For the ReAdW tool (which converts Thermo .raw files to mzXML), you need to have the same (or higher) version of Xcalibur used for the acquisition. We're bound by the Thermo license agreement and cannot redistribute the necessary Thermo library files.
For reference, http://tools.proteomecenter.org/wiki/index.php?title=Software:ReAdW#Requirements Best of luck, and please feel free to ask any other questions here once you get past this step. -Natalie On Tue, Jan 27, 2009 at 2:29 PM, lilly <[email protected]>wrote: > > I'm a very new user to this analysis tool, could anyone please help?? > When trying to convert RAW to pepXML I keep getting this message for > each RAW file. Could this be because I don't have Xcaliber or Bioworks > on my laptop? I've had to use my laptop as didn't have the admin > priviledges on our labs computer > > Thanks in advance, > > Elisabeth (PhD student) > > > ReAdW -v --mzXML c:/Inetpub/wwwroot/ISB/data/SILAC/RAW/ > elisabeth_3nov_08_01.RAW > > -------------------------------------------------------------------------------- > > Settings: > centroid scans: 0 > compress scans: 0 > verbose mode: 1 > lockspray: 1 > MS^E: 0 > threshold: 0 > mzML mode: 0 > mzXML mode: 1 > input filename: c:/Inetpub/wwwroot/ISB/data/SILAC/RAW/ > elisabeth_3nov_08_01.RAW > output filename: c:/Inetpub/wwwroot/ISB/data/SILAC/RAW/ > elisabeth_3nov_08_01.mzXML > > Unable to initialize Xcalibur 2.0 interface; falling back to 1.4 > interface > Unable to initialize XCalibur 1.4 interface; check installation > hint: try running the command "regsvr32 C:\<path_to_Xcalibur_dll> > \XRawfile2.dll" > unable to interface with Thermo library > Command FAILED > > > > --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
