Thank you, Moyez.  We appreciate your work!  By the way, I still
haven't received your file for testing.

Thanks,

Natalie


On Tue, Mar 17, 2009 at 2:38 PM, Moe <[email protected]> wrote:
>
> The memory leak appears to be isolated in class AnalystQS20Interface,
> method processMSSpecData, in the call to "m_ipGetRawDataSpec-
>>GetRawScanXYPoints()" when requesting the product spectrum's entire
> set of data points (origical code snip below).
>
>    /*** ORIGINAL CODE FROM RELEASE 4.2.0 ***/
>
>    inline void AnalystQS20Interface::processMSSpecData(Scan *pScan) {
>    #if 1
>        ...
>        long lSpecDataPoints;
>        ...
>        VARIANT dXMass; VariantInit(&dXMass);
>        VARIANT dYInts; VariantInit(&dYInts);
>        ...
>        HRESULT hr = m_ipGetRawDataSpec->GetRawScanXYPoints
> (&lSpecDataPoints, &dXMass, &dYInts);
>        ...
>        long lPeaksCount=0;
>        ...
>        for (long dpi=1; dpi<=lSpecDataPoints; dpi++, pXMass++, pYInts++) {
>                if (*pYInts>0.0) {
>                        pScan->mzArray_[lPeaksCount] = *pXMass;
>                        pScan->totalIonCurrent_ += 
> (pScan->intensityArray_[lPeaksCount]
> =*pYInts);
>                        lPeaksCount++;
>
>                        if (pScan->basePeakIntensity_<*pYInts) {
>                                pScan->basePeakMZ_ = *pXMass;
>                                pScan->basePeakIntensity_ = *pYInts;
>                        }
>                }
>        }
>        ...
>
> It appears that the memory occupied at &dXMass and &xYInts VARIANTs
> does not get freed even after descoping, probably because it is being
> written externally to mzWiff by the AnalystService process. It does
> not seem possible to free this memory internally, but I have not
> explored all options here.
>
> Perhaps an ultimate call to "m_ipGetRawDataSpec->Release" may induce
> the freeing, but I suppose this would render the IGetRawData structure
> prematurely unusable.
>
> As a workaround, I avoid the call to GetRawScanXYPoints() altogether,
> instead calling m_ipFMANSpecData->GetDataPointXValue() and -
>>GetDataPointYValue() to request an individual datapoint, as per code
> below. As this call must be made for each data point, there is a
> performance hit. I estimated it to be about 30% longer execution time
> for a 500 MB input, which is still reasonable for me at this time.
>
>        /*** WORKAROUND CODE ***/
>        ...
>        long lSpecDataPoints = m_ipFMANSpecData->GetNumberOfDataPoints();
>        ...
>        double dblX, dblY;
>        long lPeaksCount=0;
>        for (long dpi=1; dpi<=lSpecDataPoints; dpi++) {
>                dblX = m_ipFMANSpecData->GetDataPointXValue(dpi);
>                dblY = m_ipFMANSpecData->GetDataPointYValue(dpi);
>                if (dblY>0) {
>                        pScan->mzArray_[lPeaksCount] = dblX;
>                        pScan->intensityArray_[lPeaksCount] = dblY;
>                        pScan->totalIonCurrent_ += dblY;
>                        lPeaksCount++;
>
>                        if (pScan->basePeakIntensity_ < dblY) {
>                                pScan->basePeakMZ_ = dblX;
>                                pScan->basePeakIntensity_ = dblY;
>                        }
>                }
>        }
>
>
>
> On Mar 16, 6:09 pm, Moe <[email protected]> wrote:
>> I have uploaded the files bsa-20fmol-2.wiff/.scan. Although the
>> converter doesn't crash on this file, it did reach > 300 MB memory
>> usage in my attempt.
>>
>> Let me know if you need anything else for your debugging.
>>
>> Thanks,
>> Moyez
>>
>> On Mar 16, 5:46 pm, Natalie Tasman <[email protected]> wrote:
>>
>>
>>
>> >http://groups.google.com/group/spctools-discuss/web/how-to-upload-fil...
>>
>> > On Mon, Mar 16, 2009 at 2:43 PM, Moe <[email protected]> wrote:
>>
>> > > Thanks for the VC tips. Where can I upload the BSA file?
>>
>> > > Moyez
>>
>> > > On Mar 16, 5:25 pm, Natalie Tasman <[email protected]> wrote:
>> > >> Hi Moyez,
>>
>> > >> Of course we have our own files to test, but yes, please upload your
>> > >> file, as we've never observed the behavior you're reporting.
>>
>> > >> And also please rerun your conversions with the "-v" (verbose) option
>> > >> and copy and paste the output up to the "sha-1" calculation.
>>
>> > >> You're welcome to rebuild and debug the converter on your own.  Just
>> > >> open the TPP.sln file and build the mzWiff project from there.  Note
>> > >> you'll also need the "win_lib" project to be checked out at the same
>> > >> level as the TPP code for boost, zlib, etc.
>>
>> > >> -Natalie
>>
>> > >> On Mon, Mar 16, 2009 at 2:07 PM, Moe <[email protected]> wrote:
>>
>> > >> > Hi Natalie,
>>
>> > >> > The leak is apparent in QStar WIFF files of any size. For a 30 minute
>> > >> > BSA QC run (25 MB file size), peak memory usage reached 314 MB (to
>> > >> > produce ~195 MB mzXML output), which doesn't seem reasonable. Do you
>> > >> > have your own data files that you can debug with? If not, I can upload
>> > >> > the BSA run to your FTP site (need the details).
>>
>> > >> > The data I'm trying to analyze consists of 2 hour runs at about 500 MB
>> > >> > each. mzWiff crashes about halfway through a data file, leaving a 1.3
>> > >> > GB (incomplete) mzXML file which ends at an elution time of about 60
>> > >> > minutes (when memory reaches slightly > 2 GB).
>>
>> > >> > I have tried older releases of the executable up to 4.0.0 and all
>> > >> > behave the same. I am considering recompiling a 64-bit executable, or
>> > >> > trying to locate the leak. I'll check out the code from SourceForge,
>> > >> > but if you have additional instructions for setting up the VC project,
>> > >> > please let me know.
>>
>> > >> > Thanks,
>> > >> > Moyez
>>
>> > >> > On Mar 16, 1:41 pm, Natalie Tasman <[email protected]> wrote:
>> > >> >> Hello Moyez,
>>
>> > >> >> Thank you for the detailed bug report.  I haven't heard of issues 
>> > >> >> with
>> > >> >> mzWiff.  Thanks for the MSVC hint-- I'm pretty sure this is not 
>> > >> >> turned
>> > >> >> on yet, but actually probably shouldn't be necessary.  I agree with
>> > >> >> you that that much memory usage sounds like a memory leak.  The
>> > >> >> converters are designed to process data scan-by-scan with a 
>> > >> >> relatively
>> > >> >> low memory overhead.
>>
>> > >> >> If you can upload a sample file we can take a look and see if we can
>> > >> >> replicate behavior and then fix it.
>>
>> > >> >> Thanks,
>>
>> > >> >> Natalie
>>
>> > >> >> On Mon, Mar 16, 2009 at 9:11 AM, Moe <[email protected]> wrote:
>>
>> > >> >> > I'm trying to convert Analyst QStar Elite data acquired with 
>> > >> >> > Analyst
>> > >> >> > QS 2.0, with mzWiff version 4.2.0(build Feb 19 2009 09:48:08), on
>> > >> >> > Windows XP Pro SP3 and Windows Server 2003 R2 x64 Standard SP2.
>>
>> > >> >> > There appears to be a memory leak causing process memory to 
>> > >> >> > accumulate
>> > >> >> > to > 2GB, shortly after which the program crashes with error:
>>
>> > >> >> > ERROR: COM error 80004005 while processing sample#2
>> > >> >> > INFO: error message: Unspecified error
>>
>> > >> >> > This happens both on 32-bit XP and 64-bit Server 2003, both systems
>> > >> >> > have > 4GB physical memory. The per-process addressable limit for 
>> > >> >> > a 32-
>> > >> >> > bit process is 2GB unless compiled with the /LARGEADDRESSAWARE 
>> > >> >> > Visual C
>> > >> >> > ++ linker flag.
>>
>> > >> >> > Is anyone aware of this problem? Is this in fact a leak that can be
>> > >> >> > avoided?
>>
>> > >> >> > Thanks,
>> > >> >> > Moyez- Hide quoted text -
>>
>> > >> >> - Show quoted text -- Hide quoted text -
>>
>> > >> - Show quoted text -- Hide quoted text -
>>
>> > - Show quoted text -- Hide quoted text -
>>
>> - Show quoted text -
>
> >
>

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