Hi guys, I'm experiencing a problem with analyze peptide step in TPP; when I launch the peptide/protein prophet I get the errore message below, sometimes it even goes into an infinite loop giving no result at all. I'd really appreciate any hint.
Best Filippo Command 1 [Fri Jun 12 16:31:10 2009] [ Show / Hide ] run_in j:/Inetpub/wwwroot/ISB/data/enolase_DDA02; xinteract - Ninteract.pep.xml -p0.05 -l7 -Op j:/Inetpub/wwwroot/ISB/data/ enolase_DDA02/enolase_DDA02.tandem.pep.xml C:\Inetpub\tpp-bin\xinteract.exe (TPP v4.2 JETSTREAM rev 0, Build 200902190939 (MinGW)) running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "j:/ Inetpub/wwwroot/ISB/data/enolase_DDA02/enolase_DDA02.tandem.pep.xml" - L"7"" warning: xsl file j:/inetpub/wwwroot/ISB/data/enolase_DDA02/ interact.pep.xsl must begin with the WEBSERVER_ROOT path for use with a webserver. file 1: j:/Inetpub/wwwroot/ISB/data/enolase_DDA02/ enolase_DDA02.tandem.pep.xml processed altogether 406 results problem (ph8): j:/Inetpub/wwwroot/ISB/data/enolase_DDA02/ interact.pep.xml is not mounted under webserver root: c:/Inetpub/ wwwroot/ command completed in 2 sec running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" MINPROB=0.05" (X! Tandem (k-score)) init with X! Tandem (k-score) trypsin MS Instrument info: Manufacturer: Waters, Model: q-tof micro, Ionization: ESI, Analyzer: TOFMS, Detector: unknown PeptideProphet (TPP v4.2 JETSTREAM rev 0, Build 200902190939 (MinGW)) akel...@isb read in 0 1+, 203 2+, 203 3+, 0 4+, 0 5+, 0 6+, and 0 7+ spectra. Initialising statistical models ... negmean = 0.0533258 negmean = -0.898231 ...skipping 15 lines of output... command completed in 1 sec running: "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml"" command completed in 0 sec running: "C:/Inetpub/tpp-bin/RefreshParser "interact.pep.xml" "j:/ Inetpub/wwwroot/ISB/data/dbase/ HsIPI3.50_Ec_cRAP_20_TargetDecoy.fasta"" - Searching the tree... - Linking duplicate entries... - Printing results... - Building Commentz-Walter keyword tree...command completed in 10 sec running: "C:/Inetpub/tpp-bin/PepXMLViewer.cgi -I j:/Inetpub/wwwroot/ ISB/data/enolase_DDA02/interact.pep.xml" error: unable to read pepxml file: error parsing pepxml file: can't open index file for output: j:/Inetpub/wwwroot/ISB/data/enolase_DDA02/ interact.pep.xml.index; check permissions command "C:/Inetpub/tpp-bin/PepXMLViewer.cgi -I j:/Inetpub/wwwroot/ISB/ data/enolase_DDA02/interact.pep.xml" failed: Operation not permitted command "C:/Inetpub/tpp-bin/PepXMLViewer.cgi -I j:/Inetpub/wwwroot/ISB/ data/enolase_DDA02/interact.pep.xml" exited with non-zero exit code: 1 QUIT - the job is incomplete command "C:\Inetpub\tpp-bin\xinteract.exe -Ninteract.pep.xml -p0.05 - l7 -Op j:/Inetpub/wwwroot/ISB/data/enolase_DDA02/ enolase_DDA02.tandem.pep.xml" failed: Operation not permitted Command FAILED --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
