Hi Kristian,

thank you for your help. i changed the letter of the drive in the
environment variables, but when i wanted to start the gui i just
received a message that the access to the folder is denied.

i have the following setup of programs and data at the moment:

Perl / apache-Server on c:\

MS-Data i want to process: G:\Data\ESI\

tpp: G:\Inetpub\tpp-bin\

wwwroot: G:\Inetpub\wwwroot\

maybe there is any flaw in this setup already?

if anyone could give me a hint about how to edit httpd.conf and
tpp_gui.pl i would be really glad!

thanks a lot in advance,

johannes

On 12 Aug., 18:06, Kristian <[email protected]> wrote:
> From the start menu, go to Control Panel, System, Advanced,
> Environment Variables.
>
> Near the end of the list is a variable called WEBSERVER_ROOT whose
> value is c:\Inetpub\wwwroot
>
> Change the "c" to the new drive letter.  RESTART YOUR MACHINE. That
> should fix it.
>
> This was an issue on the cygwin-based TPP as well, but the Wiki does
> not have this info.
>
> On Aug 11, 6:20 am, ZeiserMHH <[email protected]> wrote:
>
> > hello,
>
> > i moved my data-folder to another drive and changed the settings for
> > the server root folder in tpp_gui.pl as well as in apache httpd.conf
> > according to
>
> >http://tools.proteomecenter.org/wiki/index.php?title=TPP:Using_Petuni...
>
> > i can access the files in the new location and perform a database
> > search with them. however, when i try running a peptide analysis with
> > them i get several error messages regarding the new location and the
> > command is not finished. instead pepxmlviewer.cgi runs at 100% cpu
> > usage and seems not to come to an end.
> > Is there any other file i need to edit except from tpp_gui.pl and
> > httpd.conf?
>
> > please also see below the output of the task with the error messages.
>
> > Thank you for your help!
>
> > best regards,
>
> > Johannes
>
> > ===============================
>
> > # Commands for session 0F2NBY3WV on Tue Aug 11 14:41:29 2009
> > # BEGIN COMMAND BLOCK
> > ###### BEGIN Command Execution ######
> > [Tue Aug 11 14:41:30 2009] EXECUTING: run_in G:/Daten/ESI/JZ/J090716/
> > Z11.D; c:\Inetpub\tpp-bin\xinteract  -NZ11.pep.xml -p0.05 -l7 -Op -X-
> > m0.2-nK,6-nR,10 -A-lRK-F-r0.2-mR166.11K134.11 G:/Daten/ESI/JZ/J090716/
> > Z11.D/Z11.tandem.pep.xml
> > OUTPUT:
>
> > c:\Inetpub\tpp-bin\xinteract (TPP v4.3 JETSTREAM rev 0, Build
> > 200908071234 (MinGW))
>
> > running: "C:/Inetpub/tpp-bin/InteractParser "Z11.pep.xml" "G:/Daten/
> > ESI/JZ/J090716/Z11.D/Z11.tandem.pep.xml" -L"7""
> > warning: xsl file g:/daten/esi/jz/j090716/Z11.D/Z11.pep.xsl must begin
> > with the WEBSERVER_ROOT path for use with a webserver.
> >  file 1: G:/Daten/ESI/JZ/J090716/Z11.D/Z11.tandem.pep.xml
> >  processed altogether 293 results
> > problem (ph8): g:/Daten/ESI/JZ/J090716/Z11.D/Z11.pep.xml is not
> > mounted under webserver root: c:/Inetpub/wwwroot/
> > command completed in 0 sec
>
> > running: "C:/Inetpub/tpp-bin/PeptideProphetParser "Z11.pep.xml"
> > MINPROB=0.05"
> >  (X! Tandem)
> > init with X! Tandem trypsin
> > MS Instrument info: Manufacturer: Bruker Daltonics, Model: esquire,
> > Ionization: ESI, Analyzer: Quadrupole Ion Trap, Detector:
>
> >  PeptideProphet  (TPP v4.3 JETSTREAM rev 0, Build 200908071234
> > (MinGW)) akel...@isb
> >  read in 0 1+, 190 2+, 102 3+, 0 4+, 0 5+, 0 6+, and 0 7+ spectra.
> > Initialising statistical models ...
> > negmean = 0.0533258
> > negmean = 1
> > negmean = -0.2
> > negmean = -0.2
> > negmean = -0.2
> > negmean = -0.2
> > negmean = -0.2
> > Iterations: .........10.........20.
> > WARNING: Mixture model quality test failed for charge (2+).
> > WARNING: Mixture model quality test failed for charge (3+).
> > model complete after 22 iterations
> > command completed in 0 sec
>
> > running: "C:/Inetpub/tpp-bin/ProphetModels.pl -i Z11.pep.xml"
> > Analyzing Z11.pep.xml ...
> > Parsing search results "G:/Daten/ESI/JZ/J090716/Z11.D/Z11 (X!
> > Tandem)"...
> >   => Total of 0 hits.
> > command completed in 1 sec
>
> > running: "C:/Inetpub/tpp-bin/XPressPeptideParser "Z11.pep.xml" -m0.2 -
> > nK,6 -nR,10"
> > command completed in 1 sec
>
> > running: "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "Z11.pep.xml" -lRK
> > -F -r0.2 -mR166.11K134.11"
> > WARNING: Found more than one variable mod on 'K'. Please make sure to
> > specify a heavy mass for this residue.
> > WARNING: Found more than one variable mod on 'R'. Please make sure to
> > specify a heavy mass for this residue.
> > command completed in 1 sec
>
> > running: "C:/Inetpub/tpp-bin/DatabaseParser "Z11.pep.xml""
> > command completed in 0 sec
>
> > running: "C:/Inetpub/tpp-bin/RefreshParser "Z11.pep.xml" "G:/Daten/ESI/
> > data/dbase/IPI/current/ipi.HUMAN.v3.62.fasta""
> >   - Searching the tree...
> >   - Linking duplicate entries...  - Printing results...
>
> >   - Building Commentz-Walter keyword tree...command completed in 4
> > sec
>
> > running: "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I g:/Daten/
> > ESI/JZ/J090716/Z11.D/Z11.pep.xml"
>
> > ===============================

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