That does sound annoying - can you read its contents?

-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of ZeiserMHH
Sent: Thursday, August 20, 2009 7:14 AM
To: spctools-discuss
Subject: [spctools-discuss] Re: data folder:different drive: problem


Hello Brian, Hello Kristian,

thank you for your help.

i tried changing drive letters and adding installations on c:, but it
just did not work. i got error messages that files in a tmp-folder
could not be found, because the location is missing, when i wanted to
start a x!tandem database search

i came to the conclusion, that it might be best to reinstall tpp and
transfer the data i want to process from its drive to c:, so after i
extracted all information i want i would move the processed data back
to its original location.
so, after installing tpp on c and moving some mzXML file in the data
folder i tried to run a x!tandem database search. it seems to work,
but there is a repeating popping up of a command-line window which
really kills my nerves :)

do you have any idea what could be the cause for this and how i could
stop it?

thanks again,

johannes


On 20 Aug., 02:02, "Brian Pratt" <[email protected]> wrote:
> Actually for these kind of customizations you should edit the
> tpp_gui_config.pl file instead of hacking the tpp_gui.pl file.  That way
> they won't get stomped when you do a software update.
>
> In your case you'd want to add these lines, somewhere in the middle of the
> tpp_gui_config.pl file after the line "# SET TPP_GUI DEFAULTS HERE":
>
> 'base_dir' => 'd:\\Inetpub\\tpp-bin\\',
> 'www_root' => 'd:/Inetpub/wwwroot/',
>
> Hope this helps,
>
> Brian
>
> -----Original Message-----
> From: [email protected]
>
> [mailto:[email protected]] On Behalf Of Kristian
> Sent: Wednesday, August 19, 2009 4:48 PM
> To: spctools-discuss
> Subject: [spctools-discuss] Re: data folder:different drive: problem
>
> The easiest fix I have found for this problem is not the most elegant,
> but it works:
>
> I have my data on my D: drive.  I created an exact clone of everything
> that is under C:/Inetpub on D:, including all the files in the tpp-bin
> folder.
> So on my D drive, I have an Inetpub folder that contains a wwwroot
> folder as well as a copy of the complete tpp-bin folder (Having a copy
> of tpp-bin is a bit of overkill, and most of the files don't need to
> be in both locations, but it's easier to have 200 MB of duplicate
> files than to parse out which files are necessary to have in the data
> drive).
>
> I keep my data in D:/Inetpub/wwwroot/data/.  Doing it this way (having
> a clone directory) makes it much easier when editing the tpp_gui.pl
> and creating the aliases.  Mine looks like this:
>
> my $www_root    = readconfig('www_root',"d:/Inetpub/wwwroot/");  #
> full path to web server root
> my $data_dir    = readconfig('data_dir',"${www_root}ISB/
> data/");          # full path to data directory top-level
>
> All you have to do is change every incidence of C to D (or in your
> case, G).
>
> On Aug 18, 5:31 am, ZeiserMHH <[email protected]> wrote:
> > just wanted to add, that i can access files in the tpp-bin folder via
> > my browser but cannot access the .pl file
>
> > however when is switch the line
>
> > (Alias /tpp-bin "G:/Inetpub/tpp-bin"
>
> > <Directory "G:/Inetpub/tpp-bin">
> >     Options Indexes MultiViews Includes
> >     AllowOverride None
> >     Order allow,deny
> >     Allow from all)
>
> > "    AddHandler cgi-script .cgi .pl
> >     ScriptInterpreterSource Registry
>
> > to
>
> > "    AddType text/html .shtml
> >     AddHandler server-parsed .shtml
>
> > (    PassEnv WEBSERVER_ROOT
> > </Directory>)
>
> > i have access to the code of the tpp_gui.pl file, so there seems to be
> > a problem with running the perl script on the server for some reason?
>
> > On 18 Aug., 13:41, ZeiserMHH <[email protected]> wrote:
>
> > > Hi Kristian,
>
> > > thank you for your help. i changed the letter of the drive in the
> > > environment variables, but when i wanted to start the gui i just
> > > received a message that the access to the folder is denied.
>
> > > i have the following setup of programs and data at the moment:
>
> > > Perl / apache-Server on c:\
>
> > > MS-Data i want to process: G:\Data\ESI\
>
> > > tpp: G:\Inetpub\tpp-bin\
>
> > > wwwroot: G:\Inetpub\wwwroot\
>
> > > maybe there is any flaw in this setup already?
>
> > > if anyone could give me a hint about how to edit httpd.conf and
> > > tpp_gui.pl i would be really glad!
>
> > > thanks a lot in advance,
>
> > > johannes
>
> > > On 12 Aug., 18:06, Kristian <[email protected]> wrote:
>
> > > > From the start menu, go to Control Panel, System, Advanced,
> > > > Environment Variables.
>
> > > > Near the end of the list is a variable called WEBSERVER_ROOT whose
> > > > value is c:\Inetpub\wwwroot
>
> > > > Change the "c" to the new drive letter.  RESTART YOUR MACHINE. That
> > > > should fix it.
>
> > > > This was an issue on the cygwin-based TPP as well, but the Wiki does
> > > > not have this info.
>
> > > > On Aug 11, 6:20 am, ZeiserMHH <[email protected]>
wrote:
>
> > > > > hello,
>
> > > > > i moved my data-folder to another drive and changed the settings
for
> > > > > the server root folder in tpp_gui.pl as well as in apache
httpd.conf
> > > > > according to
>
> >http://tools.proteomecenter.org/wiki/index.php?title=TPP:Using_Petuni...
>
> > > > > i can access the files in the new location and perform a database
> > > > > search with them. however, when i try running a peptide analysis
> with
> > > > > them i get several error messages regarding the new location and
the
> > > > > command is not finished. instead pepxmlviewer.cgi runs at 100% cpu
> > > > > usage and seems not to come to an end.
> > > > > Is there any other file i need to edit except from tpp_gui.pl and
> > > > > httpd.conf?
>
> > > > > please also see below the output of the task with the error
> messages.
>
> > > > > Thank you for your help!
>
> > > > > best regards,
>
> > > > > Johannes
>
> > > > > ===============================
>
> > > > > # Commands for session 0F2NBY3WV on Tue Aug 11 14:41:29 2009
> > > > > # BEGIN COMMAND BLOCK
> > > > > ###### BEGIN Command Execution ######
> > > > > [Tue Aug 11 14:41:30 2009] EXECUTING: run_in
> G:/Daten/ESI/JZ/J090716/
> > > > > Z11.D; c:\Inetpub\tpp-bin\xinteract  -NZ11.pep.xml -p0.05 -l7 -Op
> -X-
> > > > > m0.2-nK,6-nR,10 -A-lRK-F-r0.2-mR166.11K134.11
> G:/Daten/ESI/JZ/J090716/
> > > > > Z11.D/Z11.tandem.pep.xml
> > > > > OUTPUT:
>
> > > > > c:\Inetpub\tpp-bin\xinteract (TPP v4.3 JETSTREAM rev 0, Build
> > > > > 200908071234 (MinGW))
>
> > > > > running: "C:/Inetpub/tpp-bin/InteractParser "Z11.pep.xml"
"G:/Daten/
> > > > > ESI/JZ/J090716/Z11.D/Z11.tandem.pep.xml" -L"7""
> > > > > warning: xsl file g:/daten/esi/jz/j090716/Z11.D/Z11.pep.xsl must
> begin
> > > > > with the WEBSERVER_ROOT path for use with a webserver.
> > > > >  file 1: G:/Daten/ESI/JZ/J090716/Z11.D/Z11.tandem.pep.xml
> > > > >  processed altogether 293 results
> > > > > problem (ph8): g:/Daten/ESI/JZ/J090716/Z11.D/Z11.pep.xml is not
> > > > > mounted under webserver root: c:/Inetpub/wwwroot/
> > > > > command completed in 0 sec
>
> > > > > running: "C:/Inetpub/tpp-bin/PeptideProphetParser "Z11.pep.xml"
> > > > > MINPROB=0.05"
> > > > >  (X! Tandem)
> > > > > init with X! Tandem trypsin
> > > > > MS Instrument info: Manufacturer: Bruker Daltonics, Model:
esquire,
> > > > > Ionization: ESI, Analyzer: Quadrupole Ion Trap, Detector:
>
> > > > >  PeptideProphet  (TPP v4.3 JETSTREAM rev 0, Build 200908071234
> > > > > (MinGW)) akel...@isb
> > > > >  read in 0 1+, 190 2+, 102 3+, 0 4+, 0 5+, 0 6+, and 0 7+ spectra.
> > > > > Initialising statistical models ...
> > > > > negmean = 0.0533258
> > > > > negmean = 1
> > > > > negmean = -0.2
> > > > > negmean = -0.2
> > > > > negmean = -0.2
> > > > > negmean = -0.2
> > > > > negmean = -0.2
> > > > > Iterations: .........10.........20.
> > > > > WARNING: Mixture model quality test failed for charge (2+).
> > > > > WARNING: Mixture model quality test failed for charge (3+).
> > > > > model complete after 22 iterations
> > > > > command completed in 0 sec
>
> > > > > running: "C:/Inetpub/tpp-bin/ProphetModels.pl -i Z11.pep.xml"
> > > > > Analyzing Z11.pep.xml ...
> > > > > Parsing search results "G:/Daten/ESI/JZ/J090716/Z11.D/Z11 (X!
> > > > > Tandem)"...
> > > > >   => Total of 0 hits.
> > > > > command completed in 1 sec
>
> > > > > running: "C:/Inetpub/tpp-bin/XPressPeptideParser "Z11.pep.xml"
-m0.2
> -
> > > > > nK,6 -nR,10"
> > > > > command completed in 1 sec
>
> > > > > running: "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "Z11.pep.xml"
> -lRK
> > > > > -F -r0.2 -mR166.11K134.11"
> > > > > WARNING: Found more than one variable mod on 'K'. Please make sure
> to
> > > > > specify a heavy mass for this residue.
> > > > > WARNING: Found more than one variable mod on 'R'. Please make sure
> to
> > > > > specify a heavy mass for this residue.
> > > > > command completed in 1 sec
>
> > > > > running: "C:/Inetpub/tpp-bin/DatabaseParser "Z11.pep.xml""
> > > > > command completed in 0 sec
>
> > > > > running: "C:/Inetpub/tpp-bin/RefreshParser "Z11.pep.xml"
> "G:/Daten/ESI/
> > > > > data/dbase/IPI/current/ipi.HUMAN.v3.62.fasta""
> > > > >   - Searching the tree...
> > > > >   - Linking duplicate entries...  - Printing results...
>
> > > > >   - Building Commentz-Walter keyword tree...command completed in 4
> > > > > sec
>
> > > > > running: "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I
> g:/Daten/
> > > > > ESI/JZ/J090716/Z11.D/Z11.pep.xml"
>
> > > > > ===============================- Hide quoted text -
>
> > - Show quoted text -




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