Hi there, we recently installed TPP (v4.3 JETSTREAM rev 1, Build 200909091257 (MinGW)) on a Windows XP (SP2 machine). The data we're analysing is generated on an ABI/SCIEX QStar, which we've converted into mzXML using mzwiff.
The mzXML data converts fine in TPP (using the Tandem analysis pipeline) and the database search works fine on the resulting *.tandem file. We're able to convert these files into *.pepXML format and view them in the PepXML Viewer without any problem. We're unable however to analyse the *.pepXML data using XInteract. We'd like to run PeptideProphet then ProteinProphet, but each time we do the following error is generated: ******************************************************************************************************************** run_in c:/Inetpub/wwwroot/ISB/data; c:\Inetpub\tpp-bin\xinteract - Ninteract.pep.xml -p0.05 -l7 -O c:/Inetpub/wwwroot/ISB/data/ 6hrs_Control_Dupl1-s1.tandem.pep.xml -------------------------------------------------------------------------------- *********** c:\Inetpub\tpp-bin\xinteract (TPP v4.3 JETSTREAM rev 1, Build 200909091257 (MinGW)) running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/ Inetpub/wwwroot/ISB/data/6hrs_Control_Dupl1-s1.tandem.pep.xml" -L"7"" file 1: c:/Inetpub/wwwroot/ISB/data/6hrs_Control_Dupl1- s1.tandem.pep.xml processed altogether 3278 results results written to file c:/Inetpub/wwwroot/ISB/data/interact.pep.xml direct your browser to http://localhost/ISB/data/interact.pep.shtml command completed in 1 sec running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" MINPROB=0.05" (X! Tandem (k-score)) init with X! Tandem (k-score) trypsin MS Instrument info: Manufacturer: ABI / SCIEX, Model: unknown, Ionization: ESI, Analyzer: TOFMS, Detector: unknown -------------------------------------------------------------------------------- ...skipping 20 lines of output... -------------------------------------------------------------------------------- => Total of 0 hits. command completed in 0 sec running: "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml"" command completed in 0 sec running: "C:/Inetpub/tpp-bin/RefreshParser "interact.pep.xml" "c:/ Inetpub/wwwroot/ISB/data/PfalciparumAnnotatedProteins61.fasta"" - Searching the tree... - Linking duplicate entries... - Printing results... - Building Commentz-Walter keyword tree...command completed in 1 sec running: "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/ wwwroot/ISB/data/interact.pep.xml" command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/ wwwroot/ISB/data/interact.pep.xml" failed: Unknown error command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/ wwwroot/ISB/data/interact.pep.xml" exited with non-zero exit code: -1073741819 QUIT - the job is incomplete command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -O c:/Inetpub/wwwroot/ISB/data/6hrs_Control_Dupl1-s1.tandem.pep.xml" failed: Unknown error ******************************************************************************************************************************* Is anyone able to assist in establishing what the problem is, and the best solution to follow? If need be we can ftp the error files generated by the command, or an example *.pepXML data file (<4Mb). Thanks in advance Tabo --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
